Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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Quantitative Analysis of Rotenone and Deguelin in Biopesticides Containing Derris Extract by Ultra performance Liquid Chromatography

UPLC를 활용한 데리스 추출물 함유 유기농자재 중 Rotenone과 Deguelin 정량분석

  • Lim, Sung-Jin (Chemical Safety Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Jin-Hyo (Chemical Safety Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Choi, Geun-Hyoung (Chemical Safety Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Park, Byung-Jun (Chemical Safety Division, National Academy of Agricultural Science, Rural Development Administration)
  • 임성진 (농촌진흥청 국립농업과학원 화학물질안전과) ;
  • 김진효 (농촌진흥청 국립농업과학원 화학물질안전과) ;
  • 최근형 (농촌진흥청 국립농업과학원 화학물질안전과) ;
  • 박병준 (농촌진흥청 국립농업과학원 화학물질안전과)
  • Received : 2015.01.12
  • Accepted : 2015.02.16
  • Published : 2015.03.31
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Abstract

BACKGROUND: Three commercial biopesticides containing derris extract, which is permitted as a commercial biopesticide substances by the Environmentally-friendly Agriculture Promotion Act, have been marketed in Korea. But, the quantitative analytical method of active substances for crop protection in biopesticides containing derris extract has not known. METHODS AND RESULTS: Solid phase extraction (SPE) cartridge clean-up method for the quantitative analysis of rotenone and deguelin in biopesticides containing derris extract was developed and validated by ultra-performance liquid chromatography (UPLC). The clean-up method was established using hydrophilic lipophilic balance (HLB) SPE cartridges for the bioactive substances in biopesticides containing derris extract, and the eluate was analyzed to quantify the rotenone and deguelin by the UPLC. LOQ and recovery rates of rotenone and deguelin were 0.085 and 0.044 mg/L, 95.7 and 93.3%, respectively. The content of rotenone and deguelin in three biopesticides containing derris extract were analyzed by the developed method, the results showed 0.001-0.236 and

Keywords

References

  1. Bosire, C. M., Deyou, T., Kabaru, J. M., Kimata, D. M., & Yenesew, A. (2014). Larvicidal activities of the stem bark extract and rotenoids of Millettia usaramensis subspecies usaramensis on Adeds aegypti L. (Diptera: Culicidae). Journal of Asia-Pacific Entomology, 17(3), 531-535. https://doi.org/10.1016/j.aspen.2014.05.003
  2. Carboni, P., Sarais, G., Vargiu, S., De Luca, M. A., Garau, V. L., Ibba, A., & Cabras, P. (2008). LC-MS-MS determination of rotenone, deguelin, and rotenolone in human serum. Chromatographia, 68, 739-745. https://doi.org/10.1365/s10337-008-0830-0
  3. Dawson, V. K., & Allen, J. L. (1988). Liquid chromatographic determination of rotenone in fish, crayfish, mussels, and sediments. Journal-Association of Official Analytical Chemists, 71(6), 1094-1096.
  4. Draper, W. M., Dhoot, J. S., & Perera, S. K. (1999). Determination of rotenoids and piperonyl butoxide in water, sediments and piscide formulations. Journal of Environmental Monitoring, 1(6), 519-524. https://doi.org/10.1039/a904398e
  5. Jimenez, J. J., Bernal, J. L., del Nozal, M. J., Novo, M., Higes, M., & Llorente, J. (2000). Determination of rotenone residues in raw honey by solid-phase extraction and high-performance liquid chromatography. Journal of chromatography A, 871(1), 67-73. https://doi.org/10.1016/S0021-9673(99)01063-8
  6. Kim, H., Heo, S. W., & Lee, J. E. (2010). An analysis and implications on the consumption and consciousness situation of green consumers. The Korean Journal of Food Preservation, 27(3), 43-62.
  7. Kotze, A. C., Dobson, R. J., & Chandler, D. (2006). Synergism of rotenone by piperonyl butoxide in Haemonchus contortus and Trichostrongylus colubriformis in vitro: potential for drug-synergism through inhibition of nematode oxidative detoxification pathways. Veterinary Parasitology, 136(3), 275-282. https://doi.org/10.1016/j.vetpar.2005.11.001
  8. Lautie, E., Rozet, E., Hubert, P., & Leclercq, J. Q. (2012). Quantification of rotenone in Seeds of different species of yam bean (Pachyrhizus sp.) by a SPE HPLC-UV method. Food Chemistry, 131(4), 1531-1538. https://doi.org/10.1016/j.foodchem.2011.09.125
  9. Lee, J. W., Jin, C. L., Jang, K. C., Choi, G. H., Lee, H. D., & Kim, J. H. (2013). Investigation of commercial biopesticides and neem extract using solid phase extraction. Journal of Agricultural Chemistry and Environment, 2(4), 81-85. https://doi.org/10.4236/jacen.2013.24012
  10. Lim, S. J., Jeong, D. Y., Choi, G. H., Park, B. J., & Kim, J. H. (2014a). Quantitative analysis of matrine and oxymatrine in Sophora flavescens extract and its biopesticides by UPLC. Journal of Agricultural Chemistry and Environment, 3(2), 64-73. https://doi.org/10.4236/jacen.2014.32008
  11. Lim, S. J., Lee, J. H., Kim, J. H., Choi, G. H., Cho, N. J., & Park, B. J. (2014b). Quantitative analysis of cinnamaldehyde, cinnamylalcohol and salicylaldehyde in commercial biopesticides containing cinnamon extract using gas chromatography-Flame ionization detector. The Korean Journal of Environmental Agriculture, 33(3), 213-219. https://doi.org/10.5338/KJEA.2014.33.3.213
  12. Lim, S. J., Lee, J. H., Kim, J. H., Choi, G. H., Cho, N. J., & Park, B. J. (2014c). Determination of dimethyl disulfide, diallyl disulfide, and diallyl trisulfide in biopesticides containing Allium sativum extract by gas chromatography. The Korean Journal of Environmental Agriculture, 33(4), 237-243.
  13. Pedersen, T., & Shibamoto, T. (1999). Analysis of the naturally occurring pesticide rotenone by capillary gas chromatography. Journal of High Resolution Chromatography, 22(5), 294-296. https://doi.org/10.1002/(SICI)1521-4168(19990501)22:5<294::AID-JHRC294>3.0.CO;2-3
  14. Rao, S. A., Srinivas, P. V., Tiwari, A. K., Vanka, U. M., Rao, R. V. S., & Dasari, K. R. (2007). Isolation, characterization and chemobiological quantification of alpha-glucosidase enzyme inhibitory and free radical scavenging constituents form Derris scandens Benth. Journal of chromatography B, 855(2), 166-172. https://doi.org/10.1016/j.jchromb.2007.04.048
  15. Rich, R. P. (1996). Quinone binding sites of membrane proteins as targets for inhibitors. Pesticide science, 47(3), 287-296. https://doi.org/10.1002/(SICI)1096-9063(199607)47:3<287::AID-PS405>3.0.CO;2-B
  16. Scortichini, G., Diletti, G., Annunziata, L., Langella, V., & Calvarese, S. (2004). Determination of rotenone in honey by high performance liquid chromatography and ultraviolet detection. Veterinaria italiana, 40(2), 56-61.
  17. Shin, Y. G., Udeanin, G. O., Kosmeder II, J. W., Zhao, G. M., Dagar, S., Onyuksel, H., Moriarty, R. M., Cordell, G. A., Moon, R. C., Kinghorn, A. D., & Pezzuto, J. M. (2004). Identification of degradation product of deguelin and its stability using liquid chromatography and electrospray/mass spectrometry. Journal of liquid chromatography & related technologies, 27(6), 1057 -1069. https://doi.org/10.1081/JLC-120030178
  18. Svasti, J., Srisomsap, C., Subhasitanont, P., Keeratichamroen, S., Chokchaichamnankit, D., Ngiwsara, L., Chimnoi, N., Pisutjaroenpong, S., Techasakul, S., & Chen, S. T. (2005). Proteomic profiling of cholangiocarcinoma cell line treated with promiferin from Derris malaccensis. Proteomics, 5(17), 4504-4509. https://doi.org/10.1002/pmic.200401315
  19. Wenjie, J., Yuchun, F., Chunji, G., Yunhui, W., & Jie, P. (2009). Extraction and purification of deguelin from Derris trifoliate Lour root. International Journal of Agricultural and Biological Engineering, 2(4), 98-102.
  20. Yenesew, A., Twinomuhwezi, H., Kabaru, J. M., Akala, H. M., Kiremire, B. T., Heydenreich, M., Peter, M. G., Eyase, F. L., Waters, N. C., & Walsh, D. S. (2009). Antiplasmodial and larvicidal flavonoids from Derris trifoliate. Bulletin of the Chemical Society of Ethiopia, 23(3), 409-414.

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