• Korean Journal of Food Science and Technology
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• v.37 no.2
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• pp.255-260
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• 2005

To make Chungkuk-jang with enhanced fibrinolytic and immunostimulating activities, 220 strains isolated from Chungkuk-jangs were tested, and 13 Bacillus strains with excellent proteolytic and polysaccharide-producing activities were selected and tested for their fibrinolytic and immunostimulating activities using fibrin plate method and RAW 264.7 cell line, respectively. To assess macrophage activation, contents of cytokines such as tumor necrosis factor ($TNF-{\alpha}$) and $interleukin-1{\alpha}$ and nitric oxide were measured. Three strains showing highest fibrinolytic and immunostimulating activities were identified as Bacillus licheniformis (CHKJ 1249, 1326) and Bacillus subtilis (CHKJ 1339).

• Molecules and Cells
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• v.22 no.3
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• pp.328-335
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• 2006

Imperatoxin A ($IpTx_a$), a 3.7 kDa peptide from the African scorpion Pandinus imperator, is an agonist of the skeletal muscle ryanodine receptor (RyR1). In order to study the structure of the toxin and its effect on RyR1, $IpTx_a$ cDNA was PCR-amplified using 3 pairs of primers, and the toxin was expressed in E. coli. The toxin was further purified by chromatography, and various point mutants in which basic amino acids were substituted by alanine were prepared by site-directed mutagenesis. Studies of single channel properties by the planar lipid bilayer method showed that the recombinant $IpTx_a$ was identical to the synthetic $IpTx_a$ with respect to high-performance liquid chromatography mobility, amino acid composition and specific effects on RyR1. Mutations of certain basic amino acids ($Lys^{19}$, $Arg^{23}$, and $Arg^{33}$) dramatically reduced the capacity of the peptide to activate RyRs. A subconductance state predominated when $Lys^8$ was substituted with alanine. These results suggest that some basic amino acid residues in $IpTx_a$ are important for activation of RyR1, and that $Lys^8$ plays an important role in regulating the gating mode of RyR1.

• Molecules and Cells
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• v.43 no.3
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• pp.222-227
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• 2020

Inositol polyphosphate multikinase (IPMK) is required for the biosynthesis of inositol phosphates (IPs) through the phosphorylation of multiple IP metabolites such as IP3 and IP4. The biological significance of IPMK's catalytic actions to regulate cellular signaling events such as growth and metabolism has been studied extensively. However, pharmacological reagents that inhibit IPMK have not yet been identified. We employed a structure-based virtual screening of publicly available U.S. Food and Drug Administration-approved drugs and chemicals that identified the antidepressant, vilazodone, as an IPMK inhibitor. Docking simulations and pharmacophore analyses showed that vilazodone has a higher affinity for the ATP-binding catalytic region of IPMK than ATP and we validated that vilazodone inhibits IPMK's IP kinase activities in vitro. The incubation of vilazodone with NIH3T3-L1 fibroblasts reduced cellular levels of IP5 and other highly phosphorylated IPs without influencing IP4 levels. We further found decreased Akt phosphorylation in vilazodone-treated HCT116 cancer cells. These data clearly indicate selective cellular actions of vilazodone against IPMK-dependent catalytic steps in IP metabolism and Akt activation. Collectively, our data demonstrate vilazodone as a method to inhibit cellular IPMK, providing a valuable pharmacological agent to study and target the biological and pathological processes governed by IPMK.

• Proceedings of the Korean Vacuum Society Conference
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• 1999.07a
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• pp.120-120
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• 1999

III-nitride계 물질들은 blue와 UV 영역의 LED, LD와 같은 광소자뿐만 아니라 HBT, FET와 같은 전자소자로도 널리 응용되고 있다. 이와 같은 물질을 이용한 소자를 제작할 수 있는 낮은 저항의 ohmic contact은 필수적이다. Al이나 Ti와 같은 물질을 기초로 한 n-GaN의 경우는 이미 많은 연구결과가 발표되어 전기적 광학적 소자를 동작하는데 충분히 낮은 ohmic contact저항( )을 었다. 그러나 p-GaN의 ohmic contact은 아직까지 많은 문제점을 내포하고 있다. 그 중의 하나는 높은 doping 농도( )의 p-GaN 박막을 성장하기가 어렵다는 것이며, 또 하나는 낮은 접촉 비저항을 얻기 위해선 7.5eV이상의 큰 재가 function을 지닌 금속을 선택해야 한다. 그러나 5.5eV 이상의 재가 function을 갖는 금속은 존재하지 않는다. 위와 같은 문제점들은 p-GaN의 접촉 비저항이 이상의 높은 값을 갖게 만들고 있으며, 이에 대한 해경방안으로는 고온의 열처리를 통하여 p-GaN와 금속 사이에서 화학적 반응을 일으킴으로써 표면 근처에서 캐리어농도를 증가시키고, 캐리어 수송의 형태가 tunneling 형태로 일어날 수 있도록 하는 tunneling current mechanism을 이용하는 것이다. 이로 인해 결국 낮은 접촉 비저항을 얻을 수 있게되며, 일반적으로 p-GaN에서는 Nidl 좋은 물질로 알려져 있다. 그러나 Ni은 50$0^{\circ}C$이상의 열처리에서 쉽게 산화되는 특성 때문에 높은 캐리어를 얻는데 어려운 문제점이 있다. 이에 본 연구에서는 MBE로 성장된 p-GaN박막을 Mg의 activation을 더욱 증가시키기 위해 N2 분위기에서 15분간 90$0^{\circ}C$에서 annealing을 하였으며, ohmic 접촉을 위한 금속으로 높은 재가 function과 좋은 adhesion 그리고 낮은 자체저항을 가지고 있는 Ni/Au를 ohmic metal로 하여 contact한 후에 90$0^{\circ}C$에서 10초간 rapid thermal annealing (RTA)처리를 했다. 성장된 박막의 광학적 성질은 PL로써 측정하였으며, photoconductivity 실험을 통해 impurity의 life time을 분석하였고, persistent photoconductivity를 통해 dark current를 측정하였다. 또한 contact resistance를 계산하기 위해 circular-TLM method을 이용하여 I-V 특성을 조사하였다.

• International Journal of Fluid Machinery and Systems
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• v.9 no.1
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• pp.66-74
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• 2016

Concerning the numerical simulation of high-speed water jet with intensive cavitation this paper presents a practical compressible mixture flow method by coupling a simplified estimation of bubble cavitation and a compressible mixture flow computation. The mean flow of two-phase mixture is calculated by URANS for compressible fluid. The intensity of cavitation in a local field is evaluated by the volume fraction of gas phase varying with the mean flow, and the effect of cavitation on the flow turbulence is considered by applying a density correction to the evaluation of eddy viscosity. High-speed submerged water jets issuing from a sheathed sharp-edge orifice nozzle are treated when the cavitation number, ${\sigma}=0.1$, and the computation result is compared with experimental data The result reveals that cavitation occurs initially at the entrance of orifice and bubble cloud develops gradually while flowing downstream along the shear layer. Developed bubble cloud breaks up and then sheds downstream periodically near the sheath exit. The pattern of cavitation cloud shedding evaluated by simulation agrees experimental one, and the possibility to capture the unsteadily shedding of cavitation clouds is demonstrated. The decay of core velocity in cavitating jet is delayed greatly compared to that in no-activation jet, and the effect of the nozzle sheath is demonstrated.

• Korean Journal of Construction Engineering and Management
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• v.19 no.3
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• pp.79-88
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• 2018

Construction business VE is classified into design stage VE and construction stage VE. The faster the application, the better the effect. Therefore, the design stage VE was legislated in 2000 and actively applied. However, it is urgent to activate the construction VE, which is complementary to the design VE system. In the meantime, we have made a lot of efforts to activate construction VE by suggesting the necessity of construction VE system, improvement of technology development compensation system, concrete implementation method of construction VE, but it was not effective due to lack of related laws. This study compares and analyzes construction VE system in Korea and abroad for the activation of construction VE after recognizing such domestic reality. A survey of experts in each field of the construction industry was conducted and suggestions for improvement of construction VE system suitable for domestic construction industry were suggested.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.9
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• pp.1363-1369
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• 2013

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) can selectively induce apoptosis by targeting cancer cells. However, some cancer cells are resistant to TRAIL-induced cytotoxicity. One method of overcoming TRAIL resistance is combination treatment with reagents to sensitize cells to TRAIL. Luteolin, a flavonoid, has been shown to have anti-cancer effects by inducing apoptosis and cell cycle arrest in various cancer cell lines in vitro. In this study, we investigated the effects of combination treatment with non-toxic concentration of TRAIL and luteolin in T24 human bladder cancer cells. Combined treatment with luteolin and TRAIL significantly inhibits cell proliferation via activation of caspases by inducing Bid truncation, up-regulation of Bax and down-regulation of X-linked inhibitor of apoptosis protein (XIAP). However, the apoptotic effects of combination treatment with luteolin and TRAIL were significantly inhibited by specific caspases inhibitors. Taken together, these results indicate that combination treatment with TRAIL and luteolin can induce apoptosis in TRAIL-resistant cancer cells through down-regulation of XIAP and modulation of tBid and Bax expression.

• Proceedings of the Korean Institute of Interior Design Conference
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• 2008.05a
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• pp.116-122
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• 2008

As a model house has attempted a role change from a conceptual perspective as a housing cultural center starting from the 90ties, it began to direct the change into a high end building. In particular, large construction companies have recently invested on an unimagined scale, unlike in the past, in building a model house and pursued the construction of new attractions of cities as well as the upgrade of a brand image by hiring and implementing the designs of celebrity architects or designers. In addition, they have been in pursuit of a change by enhancing the satisfaction and pride of residents by offering spaces and programs for various cultural arts for apartment residents or potential customers and having them participate in those programs. This study aims to expound on the possibility of the utilization of a housing cultural center as a local complex community center by analyzing the spatial organization and the operational program of a housing cultural center appearing since the 90ties. With this aim, the study analyzed the cases of a complex community center and the program and the operational method of a housing cultural center within the case study in order to propose the possibilities for the utilization of a local complex community center. The elevation of the cultural and economic level of citizens and a rapid social change are shown as various demands for welfare, and the subsequent social physical change has led to the expansion of a space as a complex community. A housing cultural center is also directed to grow as a complex community center from the perspective of diverse demands and participations of consumers, and prepares a field for a broad spectrum of communications from the perspective of housing and cities.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.28 no.4
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• pp.889-903
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• 2018

Cloud computing has been activated globally due to the demands of the 4th industrial revolution and the efficient use of IT resources, and domestic usage is also increasing due to legislation and related laws. However, domestic financial companies are subject to various regulations due to the importance of their information and the ripple effects of accidents such as outflows. Only non-critical information processing systems that handle non-critical information are allowed to use cloud computing. Financial companies are required to set specific criteria and judgment to distinguish them. In this paper, we propose a method to enable the financial company cloud computing to be more active by specifying the ambiguous non - essential information processing system designation standard and making it easier to designate.

• Journal of Practical Engineering Education
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• v.8 no.1
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• pp.57-62
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• 2016

The purpose of this study is to provide the basic data and to construct education system by analizing the case of Japan on the lifelong vocational competency development utilizing the university. Japan has grown by the world leading technology and talented people. In a globalized world, In order to achieve economic growth, It is necessary to connect the Human resource development to the overall growth of the economy. After the law on policy implementation system for the Lifelong Learning Promotion is enacted. University of japan, the newly defined the philosophy of lifelong learning in the Fundamental Law of Education. Then, University of japan has induced people to actively participate in the lifelong vocational competency development. In this paper, we refer to the Shizuoka University in Japan and learning support program and we studied the method for the activation of improvement and field placement of the training capacity of field training using the case of Japan on the lifelong vocational competency development utilizing the university.