• Title/Summary/Keyword: Acremonium

Search Result 78, Processing Time 0.028 seconds

Relationship Between Fractal Dimension and Morphological Features of Cephalosporium acremonium M25 in a 30-1 Bioreactor Culture

  • Lim Jung-Soo;Kim Jung-Mo;Kim Jong-Chae;Kim Chang-Ho;Yang Dae-Ryook;Chang Hyo-Ihl;Kim Seung-Wook
    • Journal of Microbiology and Biotechnology
    • /
    • v.15 no.5
    • /
    • pp.971-976
    • /
    • 2005
  • In a 30-1 bioreactor culture, whole differentiation occurred from 48 h, and then proceeded rapidly. As swollen hyphal fragments and arthrospores increased, cephalosporin C (CPC) production increased exponentially to $1.85\;g/1^{-1}$ at 72 h. To explain the morphological changes of Cephalosporium acremonium M25 more quantitatively, specific differentiation rates and fractal analysis were employed. Specific differentiation rates of morphological factors varied greatly during the period of culture time from 48 h to 72 h, when CPC production increased significantly. Changes of fractal dimensions showed a pattern similar to that of the specific rate of arthrospores. Furthermore, it was inversely related to the specific rate of tips. Overall, it was suggested that the fractal dimension had potential for a new morphological parameter of fungal morphology, showing complex differentiation patterns.

Identification of FM001 as Plant Growth-Promoting Substance from Acremonium strictum MJN1 Culture

  • JUNG, JAE-HAN;DONG-MIN SHIN;WOO-CHUL BAE;SOON-KWANG HONG;JOO-WON SUH;SANGHO KOO;BYEONG-CHUL JEONG
    • Journal of Microbiology and Biotechnology
    • /
    • v.12 no.2
    • /
    • pp.327-330
    • /
    • 2002
  • A plant growth-promoting substance, FM001, was isolated from the culture broth of Acremonium strictum MJN1. The purification steps included solvent extraction, adsorption chromatography using Diaion HP20, TLC on silica, and HLPC using a C-18 column. The purified FM001 enhanced rice seedling growth by $11.1\%\;and\;34.0\%$ of the dried weight of the shoots and roots, and also radish growth by $26.5\%\;and\;23.7\%$ of the top length and dried weight. FM001 also significantly promoted the growth of red pepper by increasing $32.7\%$ of fruit weight and $11.3\%$ as regards the height. FM001 consisted of C, H, O, N, and S, and its molecular weight was determined to be 537.78 Da. The structure of FM001 resembled brassinosteriods, and it would appear to have great potential as an effective bio-fertilizer.

New Records of Three Sordariomycetes Fungi Isolated from Aquatic Plants in Jeju, Korea (제주지역 수생식물에서 발굴된 Sordariomycetes강 균류 3종의 국내 최초 보고)

  • Oh, Yoosun;Mun, Hye Yeon;Goh, Jaeduk;Chung, Namil
    • The Korean Journal of Mycology
    • /
    • v.46 no.2
    • /
    • pp.91-97
    • /
    • 2018
  • Three endophytic fungal strains were isolated from aquatic plants collected in Jeju. The fungi were identified by sequencing and phylogenetically analyzing their internal transcribed spacer (ITS) regions. Consequently, the three strains NNIBRFG2982, NNIBRFG2984 and NNIBRFG 3040 were confirmed as the fungal species Acremonium tubakii, Colletotrichum cliviae and Cylindrocarpon pauciseptatum, respectively, all of which are new to Korea.

Comparative analysis of silage fermentation and in vitro digestibility of tropical grass prepared with Acremonium and Tricoderma species producing cellulases

  • Khota, Waroon;Pholsen, Suradej;Higgs, David;Cai, Yimin
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.31 no.12
    • /
    • pp.1913-1922
    • /
    • 2018
  • Objective: To find out ways of improving fermentation quality of silage, the comparative analysis of fermentation characteristics and in vitro digestibility of tropical grasses silage applied with cellulases produced from Acremonium or Tricoderma species were studied in Thailand. Methods: Fresh and wilted Guinea grass and Napier grass silages were prepared with cellulases from Acremonium (AC) or Trichoderma (TC) at 0.0025%, 0.005%, and 0.01% on a fresh matter (FM), and their fermentation quality, chemical composition and in vitro digestibility were analyzed. Results: All silages of fresh Napier grass were good quality with lower pH, butyric acid, and ammonia nitrogen, but higher lactic acid content than wilted Napier grass and Guinea grass silage. Silages treated with AC 0.01% had the best result in terms of fermentation quality. They also had higher in vitro dry matter digestibility and in vitro organic matter digestibility at 6 and 48 h after incubation than other silages. Silages treated with lower levels at 0.005% or 0.0025% of AC and all levels of TC did not improve silage fermentation. Conclusion: The AC could improve silage fermentation and in vitro degradation of Guinea grass and Napier grass silages, and the suitable addition ration is 0.01% (73.5 U) of FM for tropical silage preparation.

Empirical Evaluation of Cellulase on Enzymatic Hydrolysis of Waste Office Paper

  • Park, Enoch Y.;Ikeda, Yuko;Okuda, Naoyuki
    • Biotechnology and Bioprocess Engineering:BBE
    • /
    • v.7 no.5
    • /
    • pp.268-274
    • /
    • 2002
  • Enzymatic hydrolysis of waste office paper was evaluated using three commercial cellulases, Acremonium cellulase, Meicelase, and Cellulosin T2. Varying the enzyme loading from 1 to 10% (w/w) conversion of waste office paper to reducing sugar was investigated. The conversion increased with the increase in the enzyme loading: in the case of enzyme loading of 10% (w/w), Acremonium cellulase yielded 79%conversion of waste office paper, which was 17% higher compared to Meicelase, 13% higher than that of Cellulosin T2. Empirical model for the conversion (%) of waste office paper to re-ducing sugar (x) was derived from experimental results as follow, x = $kE^{m}t^{(aE+b)}$ where k, m, a, and b de-note empirical constants. E indicates initial enzyme concentration.

Taxonomic Studies of Cellulose Decomposing Fungi Imperfecti (섬유소 분해능을 가진 불완전 균류의 분류)

  • An, Won-Gun;Lee, Jae-Dong
    • The Korean Journal of Mycology
    • /
    • v.18 no.2
    • /
    • pp.70-76
    • /
    • 1990
  • Twenty-one strains isolated, cellulose decomposing fungi, were identified on the basis of morphological, physiological and biochemical properties as Acremonium sp., Aspergillus sp., Chaetomium sp., Chrysonilla sp., Doratomyces sp., Fusarium sp., Gliomastix sp., Penicillium sp., Trichoderma sp., Varicosporium sp. and Verticillium sp.. The optimum tempeture for growth was in the range of $20-30^{\circ}C$. Most of the isolated stains utilized all tested carbon sources, and scarcely utilized urea as a nitrogen source. Only the strain No.2 had high activity of cellulase.

  • PDF

New Recorded Species in Three Genera of the Sordariomycetes in Korea

  • Park, Sangkyu;Ten, Leonid;Lee, Seung-Yeol;Back, Chang-Gi;Lee, Jae-Jin;Lee, Hyang Burm;Jung, Hee-Young
    • Mycobiology
    • /
    • v.45 no.2
    • /
    • pp.64-72
    • /
    • 2017
  • In an ongoing survey of Korean indigenous fungi, three fungal strains belonging to the Sordariomycetes were isolated from soil samples. These strains were designated KNU16-001, KNU16-002, and KNU16-009, and identified as Ambrosiella grosmanniae, Acremonium sclerotigenum, and Trichocladium asperum, respectively, based on morphological characterization and phylogenetic analysis using internal transcribed spacer region sequences of ribosomal DNA. This is the first report of these species in Korea.

Four Unreported Endophytic Fungi Isolated from Roots of Quercus spp. in Korea

  • Choi, Jae-Wook;Gwon, Ju-Hui;Park, Hyeok;Lee, Jong-Chul;Eom, Ahn-Heum
    • The Korean Journal of Mycology
    • /
    • v.49 no.3
    • /
    • pp.325-335
    • /
    • 2021
  • We isolated endophytic fungi from the roots of Quercus spp. in Korea. The isolates were identified based on morphological and molecular characteristics from the nucleotide sequences of the internal transcribed spacer and large subunit of rDNA regions. We confirmed that the four species we isolated - Oidiodendron citrinum, Acremonium crotocinigenum, Mammaria echinobotryoides, and Pleotrichocladium opacum - have not been recorded previously in Korea. Herein, we present their morphological characteristics and the result of phylogenetic analysis.

Fungal Diversity in Composting Process of Pig Manure and Mushroom Cultural Waste Based on Partial Sequence of Large Subunit rRNA

  • Cho, Kye-Man;Kwon, Eun-Ju;Kim, Sung-Kyum;Kambiranda, Devaiah M;Math, Reukaradhya K;Lee, Young-Han;Kim, Jung-Ho;Yun, Han-Dae;Kim, Hoon
    • Journal of Microbiology and Biotechnology
    • /
    • v.19 no.8
    • /
    • pp.743-748
    • /
    • 2009
  • Fungal diversity during composting was investigated by culture-independent rDNA sequence analysis. Composting was carried out with pig manure and mushroom cultural waste using a field-scale composter (Hazaka system), and samples were collected at various stages. Based on partial sequence analysis of large subunit (LSU) ribosomal RNA (rRNA) and sequence identity values, a total of 12 different fungal species were found at six sampling sites; Geotrichum sp., Debaryomyces hansenii, Monographella nivalis, Acremonium strictum, Acremonium alternatum, Cladosporium sphaerospermum, Myriangium durosai, Pleurotus eryngii, Malassezia globosa, Malassezia restricta, Rhodotorula glutinis, and Fusarium sporotrichioides. Geotrichum sp. of the class Saccharomycetes was the most predominant fungal species throughout the composting process (185 out of a total of 236 identified clones, or 78.4%), followed by Acremonium strictum (7.6%), Monographella nivalis (5.1%), and Pleurotus eryngii (3.8%). The prevalence of Geotrichum sp. was the lowest (61.1%) at the beginning of composting, and then gradually increased to 92.5% after 10 days of composting.

Biocontrol Activity of Acremonium strictum BCP Against Botrytis Diseases

  • Choi, Gyung-Ja;Kim, Jin-Cheol;Jang, Kyoung-Soo;Nam, Myeong-Hyeon;Lee, Seon-Woo;Kim, Heung-Tae
    • The Plant Pathology Journal
    • /
    • v.25 no.2
    • /
    • pp.165-171
    • /
    • 2009
  • Biological control activity of Acremonium strictum BCP, a mycoparasite on Botrytis cinerea, was examined against six plant diseases such as rice blast, rice sheath blight, cucumber gray mold, tomato late blight, wheat leaf rust, and barley powdery mildew in growth chambers. The spore suspension of strain BCP showed strong control activities against five plant diseases except against wheat leaf rust. On the other hand, the culture filtrate of A. strictum BCP was effective in controlling only cucumber gray mold and barley powdery mildew. Further in vivo biocontrol activities of A. strictum BCP against tomato gray mold were investigated under greenhouse conditions. Control efficacy of the fungus on tomato gray mold increased in a concentration-dependent manner. Treatment of more than $1{\times}10^6$ spores/ml significantly controlled the disease both in tomato seedlings and in adult plants. The high disease control activity was obtained from protective application of the strain BCP, whereas the curative application did not control the disease. Foliar infections of B. cinerea were controlled with $1{\times}10^8$ spores/ml of A. strictum BCP applied up to 7 days before inoculation. In a commercial greenhouse, application of A. strictum BCP exhibited the similar control efficacy with fungicide procymidone (recommended rate, $500{\mu}g/ml$) against strawberry gray mold. These results indicate that A. strictum BCP could be developed as a biofungicide for Botrytis diseases under greenhouse conditions.