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Four Unreported Endophytic Fungi Isolated from Roots of Quercus spp. in Korea

  • Choi, Jae-Wook (Department of Biology Education, Korea National University of Education) ;
  • Gwon, Ju-Hui (Department of Biology Education, Korea National University of Education) ;
  • Park, Hyeok (Department of Biology Education, Korea National University of Education) ;
  • Lee, Jong-Chul (Department of Biology Education, Korea National University of Education) ;
  • Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
  • Received : 2021.08.31
  • Accepted : 2021.09.23
  • Published : 2021.09.30

Abstract

We isolated endophytic fungi from the roots of Quercus spp. in Korea. The isolates were identified based on morphological and molecular characteristics from the nucleotide sequences of the internal transcribed spacer and large subunit of rDNA regions. We confirmed that the four species we isolated - Oidiodendron citrinum, Acremonium crotocinigenum, Mammaria echinobotryoides, and Pleotrichocladium opacum - have not been recorded previously in Korea. Herein, we present their morphological characteristics and the result of phylogenetic analysis.

Keywords

Acknowledgement

This work was supported by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea.

References

  1. Carroll G. Fungal endophytes in stems and leaves: from latent pathogen to mutualistic symbiont. Ecology 1988;69:2-9. https://doi.org/10.2307/1943154
  2. Bouton J, Gates R, Belesky D, Owsley M. Yield and persistence of tall fescue in the southeastern coastal plain after removal of its endophyte. Agron J 1993;85:52-5. https://doi.org/10.2134/agronj1993.00021962008500010011x
  3. Finlay R, Read D. The structure and function of the vegetative mycelium of ectomycorrhizal plants: II. The uptake and distribution of phosphorus by mycelial strands interconnecting host plants. New Phytol 1986;103:157-65. https://doi.org/10.1111/j.1469-8137.1986.tb00604.x
  4. Upson R, Read DJ, Newsham KK. Nitrogen form influences the response of Deschampsia antarctica to dark septate root endophytes. Mycorrhiza 2009;20:1-11. https://doi.org/10.1007/s00572-009-0260-3
  5. Newsham KK. A meta-analysis of plant responses to dark septate root endophytes. New Phytol 2011;190:783-93. https://doi.org/10.1111/j.1469-8137.2010.03611.x
  6. Kim CK, Eo JK, Eom AH. Diversity of foliar endophytic fungi isolated from Lindera obtusiloba in Korea. Kor J Mycol 2012;40:136-40. https://doi.org/10.4489/KJM.2012.40.3.136
  7. Kim JH, Kim DY, Park H, Eom AH. Two endophytic Diaporthe species isolated from the leaves of Astragalus membranaceus in Korea. Mycobiology 2018;35:430-3.
  8. White TJ, Bruns TD, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, editors. PCR Protocols: A Guide to Methods and Applications. London: Academic Press; 1990. p. 315-22.
  9. Vilgalys R, Hester M. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol Res 1990;172:4238-46. https://doi.org/10.1128/jb.172.8.4238-4246.1990
  10. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 2018;35:1547. https://doi.org/10.1093/molbev/msy096
  11. Schol-Schwarz M. Cephalosporium crotocinigenum sp. nov. Trans Br Mycol Soc 1965;48:51-3. https://doi.org/10.1016/s0007-1536(65)80006-7
  12. Barron G. New species and new records of Oidiodendron. Can J Bot 1962;40:589-607. https://doi.org/10.1139/b62-055
  13. Rice AV, Currah RS. Oidiodendron: A survey of the named species and related anamorphs of Myxotrichum. Stud Mycol 2005;53:83-120. https://doi.org/10.3114/sim.53.1.83
  14. Calvo M and Guarro J. Algunos dematiaceos comunes de la microflora de Cataluna. Collectanea Botanica 1979;11:92-103.
  15. Hernandez-Restrepo M. Gene J, Castaneda-Ruiz RF, Mena-Portales J. Crous PW, Guarro J. Phylogeny of saprobic microfungi from Southern Europe. Stud Mycol 2017;86:53-97. https://doi.org/10.1016/j.simyco.2017.05.002