• 대한약리학회지
• /
• 제5권2호
• /
• pp.75-79
• /
• 1969

Aconitum Koreanum rhizoma has been applied in therapy from old in oriental medicine (Korean Hanbang), The action of the water and alcohol extracts of the rhizoma and root of Aconitum Koreanum R. Raymond, a common herb in Korea, on the isolated Frog heart and $LD_{50}$ were observed. The results of the studies were as follows: 1. The cardiac effect in the concentration of the extracts$(10^{-3})$ was increased and duratrin of action prolonged approximately 10 minutes, but in the extracts $(3{\times}10^{-3})blocked$. 2. The $LD_{50}$ of extracts were 722 mg/kg (water) and 151mg/kg (alcohol) intraperitoneally in mice.

• 한국의류산업학회지
• /
• 제9권5호
• /
• pp.563-568
• /
• 2007

Water and ethanol extracts of A. koreanum were prepared and tested for their improving effects on the rat's dry skin induced by household detergent. Rats treated with ethanol extracts of A. koreanum had higher serum HDL-cholesterol and lower LDL-cholesterol levels, when compared to the ones treated with water extracts. Animals treated with ethanol extracts showed that improved protein recovery patterns over time, when it compared to the ones treated with water extracts.

• 생약학회지
• /
• 제20권1호
• /
• pp.32-36
• /
• 1989

Effects of Lycium chinensis, Epimedium koreanum and tuguaconitine which is isolated from Aconitum sibiricum on primary culture chicken embryonic brain cells were studied by microscopic observation and determined of the activity of pyruvate dehydrogenase complex(PDHC). Brain cells were prepared from the brain of 10-day-old chicken embryo and cultured with a medicine consisted of 90% Dulbecco's Modified Eagle Medium(DMEM) and 10% horse serum. It was observed that all substances studied seemed to show the tendency to stimulate the neurite outgrowth of brain cells which were cultured with a deficient medium under microscopic observation. The activity of PDHC in brain cells cultured with a deficient medium was increased by Lysium chinensis and Epimedium koreanum. However, tuguaconitine had not influence on the activity of PDHC.

• 동의생리병리학회지
• /
• 제29권1호
• /
• pp.27-32
• /
• 2015

Streptococcus mutans triggers dental caries establishment by two major factors. One is synthesis of organic acids which demineralize dental enamel and the other is synthesis of glucans which mediate the attachment of bacteria to the tooth surface. In the present study, we evaluated the effect of the ethanol extracts of Aconitum koreanum (A. koreanum ) on the growth and acid production of S. mutans. Ethanol extracts of the A. koreanum showed concentration dependent inhibitory activity against the growth and acid production of S. mutans, and produced significant inhibition at the concentration of 0.016 mg/ml compared to the control groups (p<0.05). The extracts inhibited S. mutans adherence to hydroxyapatite treated with saliva, and cell adherence was repressed by 50%, 54% at the concentration of 0.063, 0.125 mg/ml. On the study of activation of glucosyltransferase which synthesizes water insoluble glucan form sucrose, the ethanol extract of A. koreanum showed remarkable inhibition over the concentration of 0.016, 0.031, 0.063 and 0.125 mg/ml (p<0.05). Especially on the concentration of 0.063, 0.125 mg/ml, the extracts suppressed the glucan synthesis by 100%. We analyzed the component of the extracts of A. koreanum. The results showed that the extract of A. koreanum had strong phenolic compound, glycosides and organic acids. These results suggest that A. koreanum may inhibit the caries-inducing properties of S. mutans, and which may be related with strong phenolic compound, glycosides and organic acids.

• 대한약리학회지
• /
• 제8권1호
• /
• pp.15-25
• /
• 1972

Korean aconitum (Aconitum koreanum R. Raymond) as one of the botanical crude drugs which pertain to helleboraceae has been extensively applied in Chinese medicine during the past decades. It has been particularly used in immortal tonic among the folk remedies in China, however, its general uses comprehend diuresis, cardiotonic, analgesia, neuralgia, gout and, furthermore, even neoplastic effect. The components of aconitum have been acknowledged as aconitine, mesaconitine, hypaconitine, aconine and so on. The main ingredient, aconitine has the advantage of causing the atrial fibrillation, but, its pharmacological research has not been fully elucidated. Although there are many reports with regard to the pharmacological effects on the motility of several animal hearts, their conclusions have not been regretfully coincided yet. The authors hereby paid attention to this point of view and made experiment to examine the relationship between the alcohol extract of Korean aconitum and the motility of the isolated clam heart, making the use of several drugs related to the heart such as serotonin, acetylcholine, pilocarpine, physostigmine, barium chloride, procaine and quinidine. The cardiac movement of the isolated clam (Meretrix lusoria) heart in the standard sea water solution was recorded with the electric kymograph according to the Magnus method. The results of the experiment are as follows. 1. The motility of the isolated clam heart represents the tendency of gradual inhibition in proportion to the concentration of AK-A $10^{-4}$, $5{\times}10^{-4}$, and $10^{-3}$. 2. The cardiac inhibitory effect of AK-A $10^{-3}$ antagonizes the motility of the isolated clam heart pretreated with serotonin $10^{-6}$. 3. The cardiac inhibitory effect of AK-A $10^{-3}$ antagonizes the systolic state appealed by barium chloride $10^{-3}$. 4. The systolic state caused by quinidine $10^{-4}$ is not inhibited by AK-A $10^{-3}$.

• 대한본초학회지
• /
• 제32권4호
• /
• pp.33-38
• /
• 2017

Objectives : Termitomyces albuminosus (Berk.) Heim is one of the famous wild edible mushrooms in the southern part of China. It is known that Termitomyces albuminosus, like Aconitum koreanum used in Korean traditional medicine, contains a kind of cerebroside, termitomycesphin, causing a pharmacologic effect on the neuron system. The pharmacologic effect of Termitomyces albuminosus can be used to possibly replace Aconitum koreanum. However, It needs to be certified as safe before it can be used. Here, a single-oral toxicity test and safety classification was conducted to obtain acute information of the toxicity of dried-Termitomyces albuminosus powder and to secure its safety in clinical applications. Methods : In order to calculate approximate lethal dose(ALD), test substance was orally administered to male and female SD-rat at dose levels of 5,000 and 0 (vehicle control) mg/kg (body weight). Based on the result of this toxicity, also the estimation of safety classification was calculated using the HED-based (human equivalent dose) MOS (margin of safety). Results : There were no mortalities, test substances treatment-related clinical signs, no changes in the body or organ weights, and no gross or histopathological findings at 14 days after treatment with test substance. Thus, the approximate lethal dose of dried-Termitomyces albuminosus powder was considered over 5,000 mg/kg in both female and male mice. Conclusions : Based on the limit dose, 5000 mg/kg, it was estimated that dried-Termitomyces albuminosus powder is classified as "Specified class B" indicating that clinical dose is not limited to patients as safe as food.

• 생약학회지
• /
• 제7권3호
• /
• pp.211-214
• /
• 1976

Basic substances were obtained from five Korean Aconitum, A. koreanum, A. chiisanense, A. ciliae, A. pseudoproliferum and unknown Aconitum species and compared TLC patterns of the substances. Soluble substances in ammonia alkali showed 8 spots on TLC, while insoluble substances in ammonia alkali 7 spots. These patterns are all similar in all species used in the investigation.

• 동의생리병리학회지
• /
• 제22권2호
• /
• pp.346-349
• /
• 2008

To investigate whether aqueous extract from Aconitum koreanum (AEAK) effects in the process of melanin synthesis, the expression of tyrosinase-related proteins (TRPs) by immunohistochemical methods were performed in ultraviolet B (UVB) irradiated skin of guinea pig. The irradiation of UVB (60 mJ/day) was performed for 3 days and treated with AEAK for 15 days. About the color evaluation, the visual scores of UV B irradiated guinea pig with AEAK treatment were slightly lower than those in the UV B alone irradiated ones. At day 15 after UVB exposure, immunohistochemical analysis for TRPs expression were performed. The intensive expression of tyrosinase was mainly observed over epidermis with skin appendage and in the cells of dermis. Slight increase of these reaction was induced in response to UVB in the spinous and granular layer of epidermis, but similar expression in the AEAK treated guinea pig as normal one. The TRP-1 and TRP-2 expression were not detected in the skin of normal guinea pig. But intensive expression for TRP-1 and TRP-2, especially TRP-2, induced by UV B irradiation in the cells of dermis. These expressions were decreased in the AEAK treated guniea pig. Collectively, these results suggest that AEAK has a potential to inhibit synthesis through regulation of TRPs expression in the skin of guinea pig, but better understanding the function of AEAK, more research should be done in the effects of AEAK on the function of TRPs in melanogesis.

• 한국환경과학회지
• /
• 제12권2호
• /
• pp.125-132
• /
• 2003

This study was executed by China along with the Tumen river, which formed border of North Korea and China. As a result 91 family, 282 genera, 363 species, 57 variety 4 species, total 424 taxa of vascular plants were identified. The examination area was include the region that had various vegetation such as upper swampy land, seashore dune, a river, a high mountain grassy plain, deciduous tree zone, evergreen coniferous forest zone. The followings are representative plants, which found in an examination area but not in South Korea. Picea koraiensis, Larix gmelini var. principis-ruprechtii, Betula playphylla, Ulmus pumila, Populus koreana, Ledum palustre var. diversipilosum, Rhododendron parvifolium, Salix brachypoda, Phacelurus latifolius, Carex gotoi, Carex capricornis, Calla palustris, Aquilegia flabellata var. pumila, Lilium davuricum, Astragalus membranaceus, Trifolium lupinaster, Ledebourieila seselodeds, Polemonium racemosum, Scutellaria baicalensis, Linnaea borealis, Thladiantha dubia, Inula helenium, Trollius hondoensis, Hypocaeris ciliata, Aconitum kirinense, Aconitum arcuatum, Potentilla viscosa var. macrophylla. And there are 21 rare and endangered species, which Korean Forest Sevice designated, such as Aconitum koreanum, Acorus calamus var. angustatus, Polygonatum stenophyllum, Drosera rotundifolia, Iris setosa, Jeffersonia dubia, Lilium davuricum, Lilium cernum, Paeonia obovata, Penthorum chinense, Phacelurus latifolius, Rosa marretii, Sparganium stoloniferum, Thymus quinquecostatus, Trillium tschonoskii, Utricularia japonica, Tripterospermum japonicum, Vaccinium uliginosum, Vaccinium vitis-idaea, etc. Especially, plants like, Acanthopanax senticosus, Jeffersonia dubia and Lillum cernum which have high values of ornamental, and medical use are in an extinction crisis. High swampy land was formed to upper stream of Tumen river. It showed representative vegitation, like Carex sp., Scirpus radirans, Eriophorum latifolium. Wetlands plant and a dune plant were mixed in down stream area of Tumen river. On a dune, seashore plants, such as Carex kobomugi, Salsola collina, Raphanus sativus var. raphanistroides, Rosa rugosa, Scutellaria strigillosa, Linaria japonica, Phacelurus latifolius were appeared frequently.

• 한국자원식물학회지
• /
• 제12권2호
• /
• pp.125-132
• /
• 1999

본 연구는 1997년 9월부터 1998년 8월까지 지리산과 한라산 일대에 분포하는 지리산오갈피군락과 섬오갈피 -곰솔군락 자생지의 생육특성과 식생을 조사하였다. 지리산오갈피군락은 지리산 노고단(1507m)과 반야봉 방향의 임걸령재(1300m)주변과 한라산 1100고지 주변에서 군락을 이루고 있었다. 구성종중 신갈나무, 오미자, 산수구, 당단풍, 관중, 노루삼, 산꿩의 다리,구상나무, 새 등이 상재도가 비교적 높게 나타났다. 지리산과 한라산지역에서 공동으로 출현한 종은 당단풍, 관중, 작살나무, 담쟁이덩굴, 고로쇠나무, 곰취, 애기나리, 졸방제비꽃, 이삭바꽃 등이 나타났다. 섬오갈피-곰솔군락은 제주도 외도 월태부락주변에서 소규모군락을 이루고 있었다. 이 지역에는 각시마, 사위질방, 으름, 실고사리 , 마삭줄, 계요등, 여우팥, 까마귀머루, 송악 등의 만경식물이 교목과 아교목층에 곰솔, 산팽나무, 예덕나무 등을 중심으로 혼생하고 있었다. 현재 본 조사지역에 더 이상 인위적인 변화가 일어나지 않는다면 이 지역에 분포하는 지리산오갈피는 구상나무-지리산오갈피군락으로 변화가 예상된다. 조사지역의 토양환경은 섬오갈피군락에서는 pH는 6.56, 전기전도도는 0.258mm ho/cm로 높고, 지리산오갈피군락에서는 유기물함량 25.16-25.35%와 총질소 7.58-9.30mg/g으로 높았다. 한라산과 지리산의 지리산오갈피군락에서는 한라산에서 pH 5.76, 전기전도도 0.238mmho/cm, 총질소 9.30mg/g, 총인 0.126mg/g으로 높게 나타났다.