• 제목/요약/키워드: AT feeder system

검색결과 153건 처리시간 0.031초

The Investigation of Cell Culture Conditions to Maintain Chicken Embryonic Stem Cells as Totipotent Cells

  • Du, Lixin;An, Jing
    • Asian-Australasian Journal of Animal Sciences
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    • 제16권8호
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    • pp.1102-1107
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    • 2003
  • The ES cell can provide a useful system for studying differentiation and development in vitro and a powerful tool for producing transgenic animalds. To investigate the culture condition of chicken embryonic stem (CES) cells which can retain their multipotentiality or totipotency, three kinds of feeder layer cells, SNL cells, primary mice embryonic fibroblasts (PMEF) cells and primary chicken embryonic fibroblasts (PCEF) cells, were used as the feeder cells in media of DMEM supplemented with leukemia inhibitory factor (LIF), basic fibroblast growth factor (bFGF) and stem cell factor (SCF) for co-culture with blastoderm cells from stage X embryos of chicken. The alkaline phosphatase (AKP) test, differentiation experiment in vitro and chimeric chicken production were carried out. The results showed that culture on feeder layer of PMEF yielded high quality CES cell colonies. The typical CES cells clone shape revealed as follows: nested aggregation (clone) with clear edge and round surface as well as close arrangement within the clone. Strong alkaline phosphatase (AKP) reactive cells were observed in the fourth passage cells. On the other hand, the fourth passage CES cells could differentiate into various cells in the absence of feeder layer cells and LIF in vitro. The third and fourth passage cells were injected into the subgerminal cavity of recipient embryos at stage X. Of 269 Hailan embryos injected with CES cells of Shouguang Chickens, 8.2% (22/269) survived to hatching, 5 feather chimeras had been produced. This suggests that an effective culture system established in this study can promote the growth of CES cells and maintain them in the state of undifferentiated and development, which lays a solid foundation for the application of CES cells and may provide an alternative tool for genetic modification of chickens.

비접지 배전계통에서 영상전류 위상 비교에 의한 고장구간 검출 방법 (A Fault Section Detection Method for Ungrounded System Based on Phase Angle Comparison of Zero-Sequence Current)

  • 양하;최면송;이승재
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 2007년도 제38회 하계학술대회
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    • pp.31-32
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    • 2007
  • In this paper, a fault section detection method is proposed for ungrounded system in the case of a single line-to-ground fault. A conventional method is used for faulted feeder selection according to the angular relationship between zero-sequence currents of the feeders and zero-sequence voltage of the system. Fault section detection is based on the comparison of phase angle of zero-sequence current. Proposed method has been testified in a demo system by Matlab/Simulink simulations. Based on Distribution Automation System(DAS), Feeder Remote Terminal Unit(FRTU) is used to collect those necessary data, at present a demo system is under developing using Manufacturing Message Specification (MMS) in IEC61850 standard.

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Relationships Between Feed Intake Traits, Monitored Using a Computerized Feed Intake Recording System, and Growth Performance and Body Composition of Group-Housed Pigs

  • Hyun, Young;Ellis, Mike
    • Asian-Australasian Journal of Animal Sciences
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    • 제13권12호
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    • pp.1717-1725
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    • 2000
  • The objective was to determine the relationship between feed intake levels and patterns, and growth performance and body composition of barrows and gilts using automatic feed intake recording equipment (F.I.R.E.). This system records the time of visits to the feeder and the duration and size of meals for individual animals housed in groups. Ninety-six crossbred pigs were grown from $33.4{\pm}0.51$ to $109.7{\pm}1.39kg$ live weight over a 13-week period. Eight mixed-sex groups of 12 pigs were used and 4 dietary treatments were compared giving 2 pens per treatment. The dietary treatments consisted of corn-soybean meal diets with differing protein levels which ranged from 14.7% to 19% between 30 to 55 kg, from 13.3% to 16.9% between 56 and 85 kg, and from 12.3% to 16.8% for the remainder of the study. Animals were ultrasonically scanned to measure loin-eye area and backfat thickness to estimate carcass fat-free lean content at the beginning and end of the study. Barrows had higher daily feed intake than gilts (2.67 vs. 2.46 kg resp. p<0.05) which was the result of a longer feeder occupation time per visit (4.77 vs. 4.54 min, resp. p<0.05), higher feed consumption rates (30.4 vs. 29.0 g/min, resp. p<0.05), and higher feed intakes per visit (136.9 vs. 126.8 g, resp. p<0.01). Gilts had less backfat and greater loin-eye area than barrows (p<0.05). Diet had no significant effect on growth performance and had limited impact on feeding patterns. Body weight showed high correlations with ADG (r=0.74), feed intake per visit (r=0.51) and feed consumption rate (r=0.69). Positive correlation were also found between daily feed intake and feed intake per visit (r=0.45), feeder occupation time per day (r=0.56), and feed consumption rate (r=0.55), and between daily feed intake and backfat thickness (r=0.32) and feed consumption rate and loin-eye area (r=0.32). There were negative correlations between number of feeder visit per day and daily feed intake (r=-0.54), and between feed intake per visit and number of feeder visits per day (r=-0.43). However, correlations between feed intake traits and carcass traits were generally low. Visits to the feeder were greatest during the morning (0700 to 1100 h) and lowest during the evening and nighttime. These results highlight limited variation among the sexes in feeding patterns and suggest important relationships between feeding behavior and feed intake.

고속열차 투입에 따른 AT급전계통의 부하용량증가에 관한 사례연구 (A case study on the increasing load capacity of AT Feeder system with speed-up train)

  • 나연일;한성호
    • 한국철도학회:학술대회논문집
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    • 한국철도학회 2005년도 추계학술대회 논문집
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    • pp.694-698
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    • 2005
  • 전기철도에 있어서 열차의 운행속도향상은 지속적으로 추구해야할 당면한 과제이며, 이를 위해서는 철도시스템 전반에 걸친 검토가 이루어져야 한다. 열차의 고속화는 차량기술 뿐만 아니라, 선로 구축물, 신호시스템, 운영기술, 전차선로, 급전시스템 등이 상호 연계되어 종합적으로 경제성을 고려하여 추진되어야 한다. 특히, 전기철도 급전계통의 경우만 보더라도 고속열차가 기존운영선로에 투입될 경우 차량의 집전성능향상, 전압강하대책, 설비용량대책, 궤도노반, 분기기, 신호의 고속화, 전차선로 구조물 검토 등 여러 가지 기술검토가 반드시 수반되어야 한다. 본 논문에서는 이 가운데 AT급전계통에서의 신규의 고속화 열차 투입에 따른 급전계통의 기술검토에 대한 접근방안을 제시하고 실 계통을 대상으로 부하용량 변화의 정도를 예측함으로써, 향후 국내 노선의 전반에 걸쳐 적용 가능함을 입증할 수 있었다.

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대중교통 지선노선 선정기법에 관한 연구 (A Study on the Feeder Transit Route Choice Technique)

  • 배기목
    • 한국항해항만학회지
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    • 제27권4호
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    • pp.479-484
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    • 2003
  • 지선버스 노선선정의 경우, 전체 네트워크로서의 노선선정 보다는 지하철 역 등의 특정 기ㆍ종점과의 연계차원에서 단일 노선으로 취급하여 노선선정을 행함이 바람직하다. 본 연구에서는 이와 같은 관점 하에 정류장 대기시간, 환승시간 등은 고려하지 않고, 일정 기ㆍ종점을 연결하는 지선노선 선정을 위한 간편 기법을 구축하였다. 검토대상 도로망 한정, 허용노선장에 의한 후보노선의 열거, 노선평가치에 의한 최종 노선의 선정 등의 3가지 단계를 통해 지선기능으로서의 마을버스 노선을 선정하는 기법을 구축하였다.

공배양 및 산소농도가 한우 난포란의 체외발생에 미치는 영향 (The Effect of Co-culture and Oxygen Concentration on In Virto Fertilization of Follicular Oocytes in Korean Native Cattle)

  • 이재관;윤준진;황성수;윤종택;김창근;정영채
    • 한국가축번식학회지
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    • 제22권1호
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    • pp.43-50
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    • 1998
  • The effect of oxygen tension on embryonic development in co-culture was evaluated from the standpoint of the reduction of dissolved oxygen concentration by the oxygen consumption of feeder cells. Three co-culture systems using bovine oviductal epitherial cells (BOEC), African green monkey kidney cells (Vero cells) or buffalo rat liver cells (BRLC) have been compared in terms of development of bovine embryos derived from oocytes matured and fertilized in vitro. Among the co-cultured embryo, Vero cells su, pp.rted the highest developmental rate (29%) and the other two showed the similar rates. When the co-cultures were incubated in three different oxygen tension such as 5, 10, 20% oxygen atmosphere, embryos co-cultured with Vero cells at 10%-O2 resulted in the highest percentage of development. From the measurement of oxygen consumption of feeder cells, BRLC consumed 1.38 10-10 mg-O2/min/cell which was higher than 0.94 10-10 and 0.26 10-10mg-O2/min/cell for Vero cells and BOEC, respectively. Based on the oxygen consumption data, the phenomena of optimum oxygen tension required in embryo development in vitro has been analyzed, and we suggested that gas phase oxygen concentration, oxygen consumption rate of feeder cells and the number of feeder cells should be considered for the design of optimal co-culture system for effective fertilization of embryos in vitro.

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연계버스 서비스권역 결정에 관한 연구 (A Development of an Optimal Feeder-Bus Service Area)

  • 이성모
    • 대한공간정보학회지
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    • 제3권2호
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    • pp.199-211
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    • 1995
  • 본 연구는 대중교통 활성화에 초점을 맞추어 대중교통의 주축을 이루고 있는 지하철과 버스를 중심으로 연계버스 서비스 권을 설정하는 방법을 제시하였다. 연계권역설정은 지하철과 버스의 상호보완적 체계를 이루어 도시체계의 비용손실을 최소화하는데 그 목적이 있으며, 이는 지하철을 중심으로 첨두시 향도심성 대중교통이용자들이 버스로 직접 도심통행시와 인접지하철역까지 버스를 이용하여 환승으로 도심통행시의 편익을 감안한 모형과 그 해법을 제시하였으며, 권역구획은 범용 GIS Package의 IDRISI를 이용하였다.

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전기철도 AT급전방식 보호시스템 최적구성 방안 연구 (A Study on the Protection System for AT Feeding on the Electric Railway)

  • 창상훈;이형수;홍재승;김정훈
    • 한국철도학회:학술대회논문집
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    • 한국철도학회 1999년도 추계학술대회 논문집
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    • pp.344-351
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    • 1999
  • The safe operation of electro railways is greatly dependant on its protective systems. The system so-called Fault Protection Wire(FW) is now widely adapted to protect in AT feeding systems. It is connected between the feeder and trolley circuit to return the fault current to autotransfonmers at substation. This paper computed the distribution of fault currents at FW in the system and also evaluated the safety from electric shock when ground fault or flashover occur in the feeding system. The results show FW is useful to protect power supply network from fault in electric railways

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초전도 한류기를 주변압기 접지선에 설치시 배전계통의 순간전압품질 분석 (Voltage Quality Analysis in Power Distribution System with Superconducting Fault Current Limiter at Grounding Line)

  • 문종필
    • 전기학회논문지P
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    • 제62권4호
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    • pp.159-163
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    • 2013
  • In this paper, voltage quality improvement is analyzed in case of Superconducting Fault Current Limiter (SFCL) installed in grounding line of main transformer in power distribution system. First, a resistive-type SFCL model is used. Next, Korean power distribution system is modeled. Finally, when SFCL is installed in the starting point of feeder and grounding line of main transformer, voltage qualities are evaluated according to various fault locations and resistance values of SFCL using PSCAD/EMTDC. The voltage quality results in case of grounding line are compared with the voltage in case of feeder.

배전기기 전압계측 정밀도 평가시스템 개발 및 성능시험 (A Development and Performance Test of Voltage Measurement Accuracy Assessment System for Distribution Equipment)

  • 조진태;김주용;이학주;김재한
    • 조명전기설비학회논문지
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    • 제27권9호
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    • pp.83-89
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    • 2013
  • Power distribution system has been changed from radial system to closed loop or mesh system due to connection of distributed generation growth. Data from distribution equipments which are installed at distribution line is required to be accurate for the performance of DMS(Distribution Management System). This paper analyzes the voltage measurement data from distribution equipment. However, the results of the analysis are confirmed to have some errors in voltage measurement data from distribution equipment. These errors come from aging of voltage sensor in distribution equipment and inaccurate data transfer to FRTU(feeder remote terminal unit) through the controller. The main problem is that the voltage measurement data of distribution equipment can not be assessed after it's first installation at the distribution line. The voltage measurement accuracy assessment system is to assess the voltage measurement data from distribution equipment on hot-line. This study had a field test to verify the performance of system.