• Title/Summary/Keyword: ASK-1

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A pattern of cell death induced by 40 kHz ultrasound in yeast cell model (40 kHz 초음파에 의해 유도된 효모세포 모델에서 세포사멸 패턴)

  • Kim, Ji Wook;Kong, Hee Jeong;Kim, Young H.;Kang, Kwang Il
    • The Journal of the Acoustical Society of Korea
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    • v.36 no.3
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    • pp.172-178
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    • 2017
  • Ultrasound has been widely used for biological and medical applications including induction of cell death, but a precise mechanism of induced cell death by ultrasound is controversial. In this study, an irradiation system with 40 kHz ultrasound was developed for a suitable cell death test of a representative unicellular organism, yeast, and used to study the biological effect of ultrasound on inducing cell death. Potassium Iodide (KI) dosimetry was used to devise an optimal system that successfully delivers 40 kHz ultrasound and produces reactive oxygen species in a 1.5 ml Eppendorf tube. Cell death was observed in an ultrasound transmission time-dependent fashion in this system. Thermal effect during irradiation was not observable in ultrasound induced cell death. Co-treatment of 40 kHz ultrasound and hydrogen peroxide showed a synergistic effect in inducing cell death. This finding suggests that 40 kHz ultrasound is related to reactive oxygen species formation. However, NAC (N-acetyl-L-cysteine) oxygen scavenger slightly inhibited the cell death by 40 kHz ultrasound. It was also found that 40 kHz ultrasound induced cell death was slightly inhibited by inhibitors of necrosis or apoptosis (glycyrrhizin or zVAD-fmk). This study suggests that cell death induced by 40 kHz ultrasound may not be exclusively related to reactive oxygen species formation and thermal effects in irradiated yeast cells.

Influence of SNR difference on the Korean speech intelligibility in classrooms (교실에서 신호대잡음비 변이가 한국어 음성명료도에 미치는 영향)

  • Park, Chan-Jae;Jo, Sung-Min;Haan, Chan-Hoon
    • The Journal of the Acoustical Society of Korea
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    • v.38 no.6
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    • pp.651-660
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    • 2019
  • The present study aims to find out the necessary speech sound level which can satisfy with the speech intelligibility in a noisy classroom environments. For this, auralized materials were made to undertake listening tests with 27 people. Speech intelligibility tests were carried out using both Consonant-Vowel-Consonant (CVC) and Phonetically Balanced Words (PBW) methods. Signal to noise ratio was changed by 5 dB for each test. As a result, it was found that speech intelligibilities are increasing with larger Signal to Noise Ratio (SNR). It was also found that there is a lot of difference of speech intelligibilities by SNR for syllables (CVC) with the Reverberation Time (RT) of 1.5 s. However, any significant difference was not found for words (PBW) in the case with RTs of below 0.8 s. Also, it was revealed through the 2-way analysis of variance (ANOVA) test that SNR is the only attentive factor which can affect the Korean speech intelligibilities for both PBW and CVC methods. Therefore, RTs below 0.8 s could be the acoustic criteria for classroom which can minimize the effects of noise. In the case with RTs larger than 0.8 s, much larger SNR is needed to give sufficient speech intelligibility.

A Study on the Characteristics of Underwater Sound Transmission by Short-term Variation of Sound Speed Profiles in Shallow-Water Channel with Thermocline (수온약층이 존재하는 천해역에서 단기간 음속구조 변화에 따른 음향 신호 전달 변동에 관한 연구)

  • Jeong, Dong-Yeong;Kim, Sea-Moon;Byun, Sung-Hoon;Lim, Yong-Kon
    • The Journal of the Acoustical Society of Korea
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    • v.34 no.1
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    • pp.20-35
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    • 2015
  • Underwater acoustic channel impulse responses (CIR) are influenced by sound speed profile (SSP), and the variation of CIR has significant effects on the performance of underwater acoustic communication systems. A significant change of SSP can occur within a short period, which must be considered during the design of underwater acoustic modems. This paper statistically analyzes the effect of the variation of SSP on the long-range acoustic signal propagation in shallow-water with thermocline using numerical modeling based on the data acquired from JACE13 experiment near Jeju island. The analysis result shows that CIR changes variously according to the SSP and the depth of the transmitter and receiver. We also found that when the transmitter and receiver are deeper, the variation of sound wave propagation pattern is smaller and signal level becomes higher. All CIR obtained in this study show that a series of bottom reflections due to downward refraction and small bottom loss in the shallow water with thermocline can be very important factor for long-range signal transmission and the performance of underwater acoustic communication system in time varying ocean environment can be very sensitive to the variation of SSP even for a short period of time.

Interior surface treatment guidelines for classrooms according to the acoustical performance criteria (학교 교실의 음환경 기준에 따른 실내마감 방안)

  • Ryu, Da-Jung;Park, Chan-Jae;Haan, Chan-Hoon
    • The Journal of the Acoustical Society of Korea
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    • v.35 no.2
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    • pp.92-101
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    • 2016
  • There are many results in which acoustical conditions of a classroom play an important role for studying effects and academic achievement of students. However, there are very few guidelines or design proposals which could make appropriate acoustic environment when classrooms are built or renovated. The present study suggests various design proposals satisfying acoustic standards of classrooms based on theoretical calculation and acoustic field experiments. At first, minimum area of sound absorption was calculated which is required to satisfy the acoustic standard for domestic middle and high schools. Also, room acoustic measurements were carried out in order to investigate the acoustic performance of an existing classroom by changing interior finishing materials on ceiling and rear walls. As a result, it was revealed that reverberation time standard below 0.8 s can be acquired even if there is no sound absorption on ceiling which is a general practice executed in Korea. Specially, it was found that if partial area of ceiling would be treated as reflective with the ratio of sound absorption and reflection as 2:1, almost similar acoustic parameters of $C_{50}$, $D_{50}$, RASTI (Rapid Speech Transmission Index) and higher sound levels could be acquired in comparison with the case of entire sound absorption on ceiling.

Efficient Prediction of Broadband Noise of a Centrifugal Fan Using U-FRPM Technique (U-FRPM 기법을 이용한 원심팬 광대역소음의 효율적 예측)

  • Heo, Seung;Cheong, Chulung
    • The Journal of the Acoustical Society of Korea
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    • v.34 no.1
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    • pp.36-45
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    • 2015
  • Recently, a lot of studies have been made about the methods used to generate turbulent velocity fields stochastically in order to effectively predict broadband flow noise. Among them, the FRPM (Fast Random Particle Mesh) method which generates turbulence with specific statistical properties using turbulence kinetic energy and dissipation obtained from the steady solution of the RANS (Reynolds Averaged Navier-Stokes) equations has been successfully applied. However, the FRPM method cannot be applied to the flow noise problems involving intrinsic unsteady characteristics such as centrifugal fan. In this paper, to effectively predict the broadband noise generated by centrifugal fan, U-FRPM (unsteady FRPM) method is developed by extending the FRPM method to be combined with the unsteady numerical solutions of the unsteady RANS equations to generate the turbulence considered as broadband noise sources. Firstly, an unsteady flow field is obtained from the unsteady RANS equations through CFD (Computational Fluid Dynamics). Then, noise sources are generated using the U-FRPM method combined with acoustic analogy. Finally, the linear propagation model which is realized through BEM (Boundary Element Method) is combined with the generated sources to predict broadband noise at the listeners' position. The proposed technique is validated to compare its prediction result with the measured data.

Floor Impact Noise Characteristics Depending on the Experimental Conditions Using Impact Ball (실험조건에 따른 임팩트 볼의 바닥충격음 변화 고찰)

  • Lee, Won-Hak;Haan, Chan-Hoon
    • The Journal of the Acoustical Society of Korea
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    • v.30 no.2
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    • pp.92-99
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    • 2011
  • In Japan, bang machine has been considered to have problems about not only the impact force and frequency response which are different from the real impact sources such as children's jumping and running, but also damage in the wooden structure housing. Therefore, a new impactor for lower impact force to prevent demage in wooden structure housing was developed. The impact ball was adopted as the second standard impact source in JIS A 1418-2 and ISO 140-11. In the present study, floor impact sounds generated by impact ball with drop heights in four floors of mock-up building of Building Research Institute (BRI) similar to typical Japanese wooden structure housing were investigated and also compared to jumping sound. The results show that Impact ball sound dropped at 10 cm to 30 cm was most similar to jumping sound. And The impact sound levels at 250 and 500 Hz were more sensitive to drop height than other lower frequencies. The error that may occur from the difference of height of 10 cm up and down based on the standard drop height caused by the impact ball operated by human hands was approx. 1 dB or less only in its value of characteristic, but it must be carefully taken into Impact ball in the Korea Standard.

An Analysis of Interaction Types in Home Economics Pre-service Teacher's Instruction Using Advanced Flanders Verbal Interaction Analysis Method (Flanders의 언어 상호작용 분석법을 활용한 가정과 예비교사의 수업 분석)

  • Yang, Ji Sun
    • Journal of Korean Home Economics Education Association
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    • v.31 no.1
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    • pp.39-58
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    • 2019
  • The purpose of this study was to investigate the home economics pre-service teachers' verbal interactions during their teaching practicum. For this purpose, the class interactions of four pre-service teachers were recorded, and the data were analyzed using advanced Flanders' verbal interaction analysis. The major findings are as follows: First, the item with the highest proportion of occurrences consisted of the pre-service teachers' 'lectures', 'nonverbal conditions' and 'asks questions'. 'forms a positive learning atmosphere' and 'praises or encourages' exhibited fewer occurrences, and there was little 'giving direction', 'criticizing students or justifying authority'. Second, the instances of class interactions appeared in the form of 'asks questions-student talk response' or 'lecture-asks questions', and in the last class were 'ask questions-student talk initiation' and 'accepts or uses ideas of students'. Third, trends of verbal interactions tend to be generally indirect, and students' remarks have appeared acceptable and compassionate. Fourth, according to expert analysis, 'class management questions' and 'diffusion questions' have increased. Pre-service teachers can guide students through their learning activities, and students can expand their thinking through the teachers' questions. As these results demonstrate, self-study analyses of pre-service teachers and active support in field are needed.

A Study on Predictive Traffic Information Using Cloud Route Search (클라우드 경로탐색을 이용한 미래 교통정보 예측 방법)

  • Jun Hyun, Kim;Kee Wook, Kwon
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.33 no.4
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    • pp.287-296
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    • 2015
  • Recent navigation systems provide quick guide services, based on processing real-time traffic information and past traffic information by applying predictable pattern for traffic information. However, the current pattern for traffic information predicts traffic information by processing past information that it presents an inaccuracy problem in particular circumstances(accidents and weather). So, this study presented a more precise predictive traffic information system than historical traffic data first by analyzing route search data which the drivers ask in real time for the quickest way then by grasping traffic congestion levels of the route in which future drivers are supposed to locate. First results of this study, the congested route from Yang Jae to Mapo, the analysis result shows that the accuracy of the weighted value of speed of existing commonly congested road registered an error rate of 3km/h to 18km/h, however, after applying the real predictive traffic information of this study the error rate registered only 1km/h to 5km/h. Second, in terms of quality of route as compared to the existing route which allowed for an earlier arrival to the destination up to a maximum of 9 minutes and an average of up to 3 minutes that the reliability of predictable results has been secured. Third, new method allows for the prediction of congested levels and deduces results of route searches that avoid possibly congested routes and to reflect accurate real-time data in comparison with existing route searches. Therefore, this study enabled not only the predictable gathering of information regarding traffic density through route searches, but it also made real-time quick route searches based on this mechanism that convinced that this new method will contribute to diffusing future traffic flow.

A Phenomenological Study of Elementary School Teachers' System Thinking-based Science Teaching Experiences (초등학교 교사의 시스템 사고를 적용한 과학 교수 경험에 대한 현상학적 연구)

  • Kim, Hyunguk;Lee, Hyonyong
    • Journal of the Korean earth science society
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    • v.40 no.1
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    • pp.68-85
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    • 2019
  • The purpose of this study was to understand science teaching experiences of elementary school teachers who taught the system thinking-based science inquiry class. The phenomenological methods were applied to analyze four elementary teachers' meaningful experiences. The four step methods of phenomenological experience research proposed by Giorgi (1985) and interview questions developed by Seidman (1998) and Schuman (1982) were used in order to collect qualitative data. The major findings of this study were as follows: First, teachers intentionally tried to ask divergent thinking questions which promoted the system thinking in classes. The teachers used divergent thinking questions to promote their students' thinking activities and to induce students' system thinking. In addition, the receptive mood created by teachers and interactive environments had a positive effect on promoting system thinking skills. Second, teachers remarked lack of teaching and learning materials and difficulties in selecting themes of their classes in order to teach the system thinking-based science inquiry class effectively. In addition, it was very difficult for teachers to evaluate the contents and processes of students' learning correctly because there were little evaluative tools and methods readily available. The findings indicated that there were some limitations in maximizing the effects of system thinking-based science inquiry instruction due to elementary students' inappropriate process skills of inquiry activities. Findings of this study revealed significant insights about elementary school teachers' experiences regarding the system thinking-based science class.

Multi channel far field speaker verification using teacher student deep neural networks (교사 학생 심층신경망을 활용한 다채널 원거리 화자 인증)

  • Jung, Jee-weon;Heo, Hee-Soo;Shim, Hye-jin;Yu, Ha-Jin
    • The Journal of the Acoustical Society of Korea
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    • v.37 no.6
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    • pp.483-488
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    • 2018
  • Far field input utterance is one of the major causes of performance degradation of speaker verification systems. In this study, we used teacher student learning framework to compensate for the performance degradation caused by far field utterances. Teacher student learning refers to training the student deep neural network in possible performance degradation condition using the teacher deep neural network trained without such condition. In this study, we use the teacher network trained with near distance utterances to train the student network with far distance utterances. However, through experiments, it was found that performance of near distance utterances were deteriorated. To avoid such phenomenon, we proposed techniques that use trained teacher network as initialization of student network and training the student network using both near and far field utterances. Experiments were conducted using deep neural networks that input raw waveforms of 4-channel utterances recorded in both near and far distance. Results show the equal error rate of near and far-field utterances respectively, 2.55 % / 2.8 % without teacher student learning, 9.75 % / 1.8 % for conventional teacher student learning, and 2.5 % / 2.7 % with proposed techniques.