• 생명과학회지
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• 제15권4호
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• pp.561-565
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• 2005

본 연구는 한국산 무 및 그 종자단백질을 추출 및 분리하여 3T3 세포주에 대해 항유전독성과 세포독성에 미치는 영향과 항균활성을 검토하였다. 3T3 세포주에 직접발암원인 $100{\mu}g/ml$ MNNG를 $10{\mu}l$씩 투여한 후 10mg/ml의 무추출물(RJ)과 1mg/ml의 종자단백질(RSP)을 $10{\mu}l$씩 투여하였을 때, 각각 $43\%$와 $30\%$의 항유전독성을 보였다. 또한 lactose dehydrogenase (LDH) 정량 분석을 이용하여, 3T3 세포주에 대한 다양한 농도의 RJ 및 RSP를 투여한 후 세포독성을 관찰 한 결과 세포독성은 관찰되지 않았다. 한편 이미 오염된 화장품에 대해 10 mg/ml 의 RJ와 1mg/ml의 RSP를 투여하였을 때, 세균성오염화장품 시료(C1)에 대해서 RSP가 $85\%$의 항균활성을 보였으며 진균성오염화장품 시료(C4)에 대해서 RSP는 $79\%$, RJ는 $76\%$의 항균활성을 보였다.

• 한국산학기술학회논문지
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• 제12권12호
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• pp.5622-5632
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• 2011

이 논문은 Automated Guided Vehicle(AGV)를 이용하여 운영되는 통합물류시스템의 시뮬레이션 실험설계와 분석에 관한 방법을 제시한다. 물류창고의 AGVs(Automated Guided Vehicle system) 성능을 최대로 운영하기 위해선 많은 변수들이 고려되어져야 하는데 대표적 중요 요인에는 차량 대수, 속도, 운행규칙, 부품 타입, 스케줄링, 버퍼 사이즈 등이 있다. 우리는 이 논문에서 다양한 중요요인들 중 (1)처리량 최대화, (2)차량 이용률 최대화 (3)차량 혼잡 최소화, (4)Automated Storage and Retrieval System(ASRS) 이용률 최대화를 고려하기 위해 직교배열(Orthogonal Array)로 실험계획을 수립하였고 이를 이용한 시뮬레이션 기반 분석과 진화전략(Evolution Strategy : ES)를 이용한 최적화를 각각 수행했다. 그 결과 ES에 비해 직교배열이 실험 시간과 회수를 절약하였고 두 결과에 대한 유효성 검사 또한 큰 차이를 나타내지 않았다. 따라서 본 논문에서 제시한 방법을 이용한 분석 방법은 시간, 회수 그리고 실험의 정확성에 대한 분석의 효율성을 증대시킬 것으로 예상되며 통합 물류 시스템 이외의 시스템에도 적용이 가능 할 것으로 생각된다.

• 대한원격탐사학회지
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• 제20권2호
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• pp.103-116
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• 2004

지표 온도를 산출하는 중규모 대기 모형은 일반적으로 태양 복사의 직달 일사량에 대하여 경사진 지형의 효과를 고려하지 않는다. 이는 실제 태양 복사량의 국지적 차이를 나타내지 못하며, 그 결과 국지적인 지표 온도에서의 큰 오차를 가져온다. 따라서 지형의 기하학적 특성뿐만 아니라 지표의 식생 특성을 고려함으로써, 정확한 국지적 지표 온도를 나타내고자 하였다. 본 연구의 목적은 GIS의 그리드 모형을 적용한 에너지 균형 모형(EBM)을 사용하여 진단적으로 지역적 지표 온도를 산출하는 것이다. 본 연구에서, 한반도 남부 지역의 지형적 비균질성은 그리드 내의 지형 방위각과 경사각의 함수인 조도각의 항으로써 흡수된 지표 일사량 계산에 고려되었다. 또한 지표 온도 변화에 주요한 변수가 되는 지표의 식생 특성이 NDVI의 항으로 사용하였다. 지표의 경사진 지형의 효과와 식생 특성이 고려된 지표 온도의 상세한 지역적 분포가 연구 결과로써 제시되었다. 이러한 지역적 지표 온도 분포는 저층 대기에서 지형에 의한 지역 순환을 형성할 수 있으며, 실제 자연에서의 지역 순환을 더욱 잘 설명할 수 있을 것이다.

• Biomolecules & Therapeutics
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• 제27권5호
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• pp.492-501
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• 2019

Nitrogen-containing heterocycles such as quinoline, quinazolinones and indole are scaffolds of natural products and have broad biological effects. During the last years those structures have been intensively synthesized and modified to yield new synthetic molecules that can specifically inhibit the activity of dysregulated protein kinases in cancer cells. Herein, a series of newly synthesized isoquinolinamine (FX-1 to 8) and isoindoloquinazolinone (FX-9, FX-42, FX-43) compounds were evaluated in regards to their anti-leukemic potential on human B- and T- acute lymphoblastic leukemia (ALL) cells. Several biological effects were observed. B-ALL cells (SEM, RS4;11) were more sensitive against isoquinolinamine compounds than T-ALL cells (Jurkat, CEM). In SEM cells, metabolic activity decreased with $10{\mu}M$ up to 26.7% (FX-3), 25.2% (FX-7) and 14.5% (FX-8). The 3-(p-Tolyl) isoquinolin-1-amine FX-9 was the most effective agent against B- and T-ALL cells with IC50 values ranging from 0.54 to $1.94{\mu}M$. None of the tested compounds displayed hemolysis on erythrocytes or cytotoxicity against healthy leukocytes. Anti-proliferative effect of FX-9 was associated with changes in cell morphology and apoptosis induction. Further, influence of FX-9 on PI3K/AKT, MAPK and JAK/STAT signaling was detected but was heterogeneous. Functional inhibition testing of 58 kinases revealed no specific inhibitory activity among cancer-related kinases. In conclusion, FX-9 displays significant antileukemic activity in B- and T-ALL cells and should be further evaluated in regards to the mechanisms of action. Further compounds of the current series might serve as templates for the design of new compounds and as basic structures for modification approaches.

• Journal of Nutrition and Health
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• 제54권5호
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• pp.448-458
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• 2021

본 연구는 뽕나무 열매 착즙 분말 (MJ)이 지방세포 분화과정에서 염증에 관여하는 유전자 발현 및 miR-132/143 조절에 미치는 효과에 중점을 두고 조사하였다. MJ는 3T3-L1 지방세포 분화 동안 지질축적이 억제되었고 PPAR-γ, CEBP-α 및 aP2와 같은 지방형성 유전자 발현을 억제하였다. 또한 염증 조절 매개체인 TNF-α, IL-6, MCP-1 및 iNOS 유전자 발현이 MJ 처리에 의해 감소되었다. MJ의 지질 축적 억제 효과는 염증과 지방분화에 관여하는 miR-132/143의 발현 감소와 관련이 있음을 확인하였다. 따라서 MJ는 염증을 동반하는 비만 예방 및 치료에 도움이 될 수 있는 식품 소재로서 가능성이 있음을 시사하고 있다. 그러나 MJ의 주요 성분인 안토시아닌의 생체이용율은 1-2% 미만으로 추정되며 예상되는 표적 조직이나 혈류에서 미량 검출되는 것으로 알려져 있다 [33]. 이를 해결하기 위해서는 생체 내 동물실험을 통한 다각적인 분석이 향후 진행되어야 할 것으로 판단된다.

• Nuclear Engineering and Technology
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• 제54권1호
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• pp.318-325
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• 2022

In this study, composites containing undoped and barium-doped Bi₂WO₆:Ba²⁺were investigated for their shielding against diagnostic X-ray. At first, Bi₂WO₆ and barium-doped Bi₂WO₆ were synthesized with different weight percentages of barium oxide through a hydrothermal process. The as-synthesized nanostructures were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS) and Raman spectroscopy (RS). After that, some shields were generated with undoped and barium-doped Bi₂WO₆:Ba²⁺ nanostructure particles incorporated into polyvinyl chloride (PVC) polymer with different thicknesses and 15% weight of the nanostructure. Finally, the prepared samples were exposed to an X-ray tube at 40, 80, and 120 kV voltages, 10 mAs and, 44.5 cm SID (i.e. the distance from the X-ray beam source to the specimen). Linear and mass attenuation coefficients were also calculated for different samples. The results indicated that, among the samples, the one with 7.5 mmol barium-doped Bi₂WO₆ had the most attenuation at the voltage of 40kV, and the attenuation coefficients would increase with an increase in the amount of barium. The samples with 15 and 17.5 mmol barium-doped Bi₂WO₆ had higher attenuation than the others at 80 and 120 kV. Moreover, the half-value layer (HVL), tenth-value layer (TVL) and 0.25 mm lead equivalent thickness were calculated for all the samples. The lowest HVL value was for the sample with 7.5 mmol barium-doped Bi₂WO₆. As the result clearly show, an increment in the barium-doping content leads to a decrease in both HVL and TVL. In every three voltages, 0.25 mm lead equivalent thickness of the barium-doped composites (7.5 mmol and 15 mmol) had less than the other composites. The lowest value of 0.25 mm lead equivalent thickness was 7.5 barium-doped in 40 kV voltage and 15 mmol barium-doped in 80 kV and 120 kV voltages. These results were obtained only for 15% weight of the nanostructure.

• Archives of Pharmacal Research
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• 제21권6호
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• pp.645-650
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• 1998

The dual drug-loaded alginate beads simultaneously containing drug in inner and outer layers were prepared by dropping plain (single-layered) alginate beads into $CaCl_2$ solution. The release characteristics were evaluated in simulated gastric fluid for 2 h followed by intestinal fluids thereafter for 12 h. The surface morphology and cross section of dual drug-loaded alginate beads was also investigated using scanning electron microscope (SEM). The poorlv water-soluble ibuprofen was chosen as a model drug. The surface of single-layered and dual drug-loaded alginate beads showed very crude and roughness, showing aggregated particles, surface cracks and rough crystals. The thickness of dual drug-loaded alginate beads surrounded by outer layer was ranged from about 57 to 329mcm. The distinct chasm between inner and outer layers was also observed. In case of single-layered alginate bead, the drug was not released in gastric fluid but was largely released in intestinal fluid. However, the release rate decreased as the reinforcing $Eudragit^{\circledR}$ polymer contents increased. When the plasticizers were added into polymer, the release rate largely decreased. The release rate of dual drug-loaded alginate beads was stable in gastric fluid for 2 h but largely increased when switched in intestinal fluid. The drug linearly released for 4 h followed by another linear release thereafter, showing a distinct biphasic release characteristics. There was a difference in the release profiles between single-layered and dual drug-loaded alginate beads due to their structural shape. However, this biphasic release profiles were modified by varying formulation compositions of inner and outer layer of alginate beads. The release rate of dual drug-loaded alginate beads slightly decreased when the outer layer was reinforced with $Eudragit^{\circledR}$ RS1OO polymers. In case of dual drug-loaded alginate beads with polymer-reinforced outer layer only, the initial amount of druc released was low but the initial release rate (slope) was higher due to more swellable inner cores when compared to polymer-reinforced inner cores. The current dual drug-loaded alginate beads may be used to deliver the drugs in a time dependent manner.

• 한국가금학회지
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• 제29권4호
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• pp.279-286
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• 2002

본 연구는 오골계, 오골계교잡종(3원교잡)육의 한약제 첨가에 따른 저장시험에서는 한약증탕액구와 오골계증탕액구, 오골계교잡종증탕액구 0.1% 효소 처리한 오골계교잡종증탕 액구를 37$^{\circ}C$에서 56일간 저장하면서 TBARS, VBN, pH, 미생물수, 관능평가 등을 조사하여 적정 저장기간을 구명하고자 하였다. 증탕액의 저장기간 설정에서는 저장기간 중 성분변화를 측정하기 위하여 레토르트 포장지에 밀봉한 한약증탕액, 오골계증탕액, 오골계교잡종증탕액, 오골재래계를 flavourzyme으로 가수분해하여 제조한 증탕액을 37$^{\circ}C$에서 저장하면서TBhRS, VBN, pH, 미생물수, 관능평가를 실험한 결과는 다음과 같았다. 지방산패도는 대부분의 처리구에서 42일까지는 유의적인 차이가 나타나지 않았으나 56일째에 효소처리한 오골계 처리구에서 TBARS 값이 유의적으로 증가하였다. 단백질 변성도는 저장기간이 경과함에 따라 증가하는 경향을 나타내었으며, 특히 저장 56일째에 한약증탕액에 비해오골계, 오골계교잡종 및 효소처리 오골계교잡종증탕액에서 유의적으로 높은 값을 나타냈다. 관능평가에 있어서는 저장 42일부터 전체기호도에 있어서 변화를 보였다. 따라서, 증탕액의 저장은 37$^{\circ}C$에서 6주가 적정기간으로 사료된다.

• Journal of Chest Surgery
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• 제10권2호
• /
• pp.241-249
• /
• 1977

Atrial septal defect is one of the most frequently encountered congenital heart disease. Up to December 31, 1976, 1682 cardiac patients received cardiac catheterization in the cardiac department of Yonsei university medical college. Out of the 1682 cardiac patients 723 cases had congenital heart disease and only 116 cases had congetial atrial septal defect. This amounted to 16.04% of all those with congenital heart disease. 58 cases of congenital atrial septal defect operated in the chest surgery department were presented. Of these 58 cases of atrial septal defect, 27 cases were male and 31 cases were female. Their ages ranged from 5 years to 54 years. The systolic pressure of the main pulmonary artery of 40 out of the 58 cases of atrial septal defect was below 40% of that of the systemic blood pressure: in 6 cases, the range of the systolic pressure of the main pulmonary artery was 50-90mmHg; in 12 cases, the range of the systolic pressure of the main pulmonary artery was 40-50mmHg. Average age of these was 30. 1 years. This study tends to show that Korean patients with atrial septal defect even though younger have a slight higher systolic pressure of the main pulmonary artery than Western patients have. The pulmonary blood is 1.5-2.5 times of systemic blood flow in 52 cases out of 58 cases of atrial septal defect.In only one of the 58 cases of atrial septal defect, the Rp was found to be as high as 45% of Rs. All other cases were below this level.51 cases had ostium secundum defect, 4 out of these cases had ostium secundum defect combined with mitral incompetence and 6 out of them had double ostium secundum defect. The remaining 7 cases had ostium primum defect. Their atrial defects were repaired under direct vision utilizing extracorporeal circulation, by hemodilution technic combined with moderate hypothermia. 44 cases [2nd atrial septal defect] were repaired by direct sutures while 14 cases, including the 7 cases ostium primum defects needed patches [1 pericardium and 13 teflon patch]. In 4 cases there were single defects while showed two defects. However the associated septal defect was so small that it could be closed by direct sutures. The size of the defect ranged between 6.0cm2and 10.0cm2 in 19 cases[33.7%]: the smallest being 0. 5cm2 and the largest 24cm2. The surgical mortality was 2 cases [3.4%]. These one case with ostium primum defect, could not be resuscitated on operation table. The cause of death in this case was myocardial failure and MI. The other, a case of ostium primum defect had a second operation on the first operative day due to massive bleeding from LV vent-line insertion site.The patient died on 26th post-operative day due to sepsis.

• Journal of Chest Surgery
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• 제10권2호
• /
• pp.230-240
• /
• 1977

Atrial septal defect is one of the most frequently encountered congenital heart disease. Up to December 31, 1976, 1682 cardiac patients received cardiac catheterization in the cardiac department of Yonsei university medical college. Out of the 1682 cardiac patients 723 cases had congenital heart disease and only 116 cases had congetial atrial septal defect. This amounted to 16.04% of all those with congenital heart disease. 58 cases of congenital atrial septal defect operated in the chest surgery department were presented. Of these 58 cases of atrial septal defect, 27 cases were male and 31 cases were female. Their ages ranged from 5 years to 54 years. The systolic pressure of the main pulmonary artery of 40 out of the 58 cases of atrial septal defect was below 40% of that of the systemic blood pressure: in 6 cases, the range of the systolic pressure of the main pulmonary artery was 50-90mmHg; in 12 cases, the range of the systolic pressure of the main pulmonary artery was 40-50mmHg. Average age of these was 30. 1 years. This study tends to show that Korean patients with atrial septal defect even though younger have a slight higher systolic pressure of the main pulmonary artery than Western patients have. The pulmonary blood is 1.5-2.5 times of systemic blood flow in 52 cases out of 58 cases of atrial septal defect.In only one of the 58 cases of atrial septal defect, the Rp was found to be as high as 45% of Rs. All other cases were below this level.51 cases had ostium secundum defect, 4 out of these cases had ostium secundum defect combined with mitral incompetence and 6 out of them had double ostium secundum defect. The remaining 7 cases had ostium primum defect. Their atrial defects were repaired under direct vision utilizing extracorporeal circulation, by hemodilution technic combined with moderate hypothermia. 44 cases [2nd atrial septal defect] were repaired by direct sutures while 14 cases, including the 7 cases ostium primum defects needed patches [1 pericardium and 13 teflon patch]. In 4 cases there were single defects while showed two defects. However the associated septal defect was so small that it could be closed by direct sutures. The size of the defect ranged between 6.0cm2and 10.0cm2 in 19 cases[33.7%]: the smallest being 0. 5cm2 and the largest 24cm2. The surgical mortality was 2 cases [3.4%]. These one case with ostium primum defect, could not be resuscitated on operation table. The cause of death in this case was myocardial failure and MI. The other, a case of ostium primum defect had a second operation on the first operative day due to massive bleeding from LV vent-line insertion site.The patient died on 26th post-operative day due to sepsis.