• Journal of Ginseng Research
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• 제48권3호
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• pp.298-309
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• 2024

Background: 20(S)-ginsenoside Rh2(GRh2), an effective natural histone deacetylase inhibitor, can inhibit acute myeloid leukemia (AML) cell proliferation. Lactate regulated histone lactylation, which has different temporal dynamics from acetylation. However, whether the high level of lactylation modification that we first detected in acute promyelocytic leukemia (APL) is associated with all-trans retinoic acid (ATRA) resistance has not been reported. Furthermore, Whether GRh2 can regulate lactylation modification in ATRA-resistant APL remains unknown. Methods: Lactylation and METTL3 expression levels in ATRA-sensitive and ATRA-resistant APL cells were detected by Western blot analysis, qRT-PCR and CO-IP. Flow cytometry (FCM) and APL xenograft mouse models were used to determine the effect of METTL3 and GRh2 on ATRA-resistance. Results: Histone lactylation and METTL3 expression levels were considerably upregulated in ATRA-resistant APL cells. METTL3 was regulated by histone lactylation and direct lactylation modification. Overexpression of METTL3 promoted ATRA-resistance. GRh2 ameliorated ATRA-resistance by downregulated lactylation level and directly inhibiting METTL3. Conclusions: This study suggests that lactylation-modified METTL3 could provide a promising strategy for ameliorating ATRA-resistance in APL, and GRh2 could act as a potential lactylation-modified METTL3 inhibitor to ameliorate ATRA-resistance in APL.

• The Journal of Advanced Prosthodontics
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• 제7권3호
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• pp.249-256
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• 2015

PURPOSE. The aim of this study was to evaluate the effects of atmospheric plasma (APL) versus conventional surface treatments on the adhesion of self-adhesive resin cement to Ti-6Al-4V alloy. MATERIALS AND METHODS. Sixty plates of machined titanium (Ti) discs were divided into five groups (n=12): 1) Untreated (CNT); 2) Sandblasted (SAB); 3) Tribochemically treated (ROC); 4) Tungsten CarbideBur (TCB); 5) APL treated (APL). SEM analysis and surface roughness (Ra) measurements were performed. Self-adhesive resin cement was bonded to the Ti surfaces and shear bond strength (SBS) tests, Ra and failure mode examinations were carried out. Data were analyzed by one-way analysis of variance and chi-squared test. RESULTS. The lowest SBS value was obtained with CNT and was significantly different from all other groups except for APL. The ROC showed the highest SBS and Ra values of all the groups. CONCLUSION. It was concluded that the effect of APL on SBS and Ra was not sufficient and it may not be a potential for promoting adhesion to titanium.

• 약학회지
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• 제43권4호
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• pp.474-480
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• 1999

The purpose of this study is to define whether Asterina pectinifera Lectin (APL) is effective on the cytokine production. Isolated mRNA from hPBMC (human peripheral blood mononuclear cells) stimulated with APL for various reaction times (1 to 96 hours) was detected by RT-PCR. The intensity of band for IL-1 and $IFN{\gamma}$ mRNA was markedly increased at l hour, and IL-2 mRNA was strongly expressed at 4 hours. The mRNA band of APL-induced IL-2 and $IFN{\gamma}$ was weaker than that of IL-1, IL-6 and $TNF{\alpha}$. The mRNA expression of 4 cytokines (IL-1, IL-2, $IFN{\gamma}$ and $TNF{\alpha}$) was detected up to 48 hours, and that of IL-6 was detected until 72 hours. ELISA was used to look protein secretion of the cytokine gene with IL-1, IL-2 and TNF$\alpha$expressed strongly in RT-PCR. The highest protein secretion was at 4 hours with IL-1, at 8 hours with IL-2 and at 4 hours with $TNF{\alpha}$. These results suggest that APL can induce the production of some cytokines and the immune response from PBMC was done within the first few hours of stimulation with APL.

• 한국조명전기설비학회지:조명전기설비
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• 제25권6호
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• pp.50-53
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• 2011

• 한국멀티미디어학회논문지
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• 제8권10호
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• pp.1360-1368
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• 2005

플라즈마 디스플레이 패널에서는 표현 가능한 회색 준위의 수를 향상시키기 위해 오차 확산 방법이 주로 사용되고 있다. 이 방법은 표현 가능한 회색 준위 수의 증가를 가져오나 오차 확산 과정에서 발생하는 웜라이크 패턴은 오차 확산 처리 이후의 여러 가지 영상 처리 과정을 거치면서 증가되어 화질저하의 요인이 된다. 본 논문에서는 플라즈마 디스플레이 패널의 화질저하를 일으키는 웜라이크 패턴의 증가를 막기 위하여 average picture level 특성을 고려한 화질개선 방법을 제안하였다. 제안한 방법으로 모의실험을 한 결과, 플라즈마 디스플레이 패널의 표현 가능한 회색 준위의 수가 증가되었고, 웜라이크 패턴은 현저히 감소하였으며, 각 회색 준위의 상관 색온도 특성이 균일하게 됨을 확인하였다.

• 한국콘텐츠학회논문지
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• 제12권2호
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• pp.339-348
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• 2012

급성 전골수구성 백혈병(acute promyelocytic leukemia, APL)은 치료제가 한정적이고 그 또한 다양한 부작용을 초래한다. 최근 암세포 형성 억제에 세균 추출물을 사용하는 경우가 증가하는데 이를 이용하여 기존의 약제보다 효과적이면서 부작용이 적은 치료제 개발이 필요하다. 본 연구에서는 L. monocytogenes에서 분비되는 물질(LmSup)과 세균 자체가 함유하고 있는 물질(LmE)을 추출하여 HL-60 세포에 처리한 다음 세포증식 억제 효과를 보고자 하였다. 세포 생존율 및 세포고사를 확인하여 세포를 죽음으로 유도하는 지 파악한 다음 작용기전을 규명하고자 세포주기의 변화 및 ROS 생성을 관찰하였다. 그 결과, LmSup와 LmE가 급성 전골수구성 백혈병(APL) 세포인 HL-60의 세포고사를 유도하고, sub G0/G1기 증가로 세포주기를 비정상적으로 차단함으로써 세포고사를 유도함을 확인하였다. 이때, ROS가 관여함을 관찰하였다. 이를 통해, LmSup 또는 LmE의 구체적인 항암효과 및 기전 분석을 통해 난치병인 APL의 치료 방법 및 치료제 개발에 기여하고자 한다.

• Journal of Microbiology
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• 제38권4호
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• pp.239-244
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• 2000

The cats gene encodes the major catalase in Sreptomyces coelicolor, whose production increases upon H$_2$O$_2$treatment. Besides the previously identified primary promoter (catApl), a minor promoter (catAp2) was newly assigned by S1 nuclease mapping. The catAp2 transcript was observed transiently upon entry into the stationary phase in liquid culture and upon differentiation on solid plates, whereas the level of catApl transcription did not chance significantly during this growth transition. ThecatApl promoter was transcribed by the major vegetative RNA polymerase holoenzyme containing $\sigma$$\^$HrdB/, whereas the catAp2 was transcribed in vitro by the holoenzyme containing $\sigma$$\^$R/ that is activated under oxidative conditions. The cia-element regulating the H$_2$O$_2$-inducibility of catApl was identified within the 23 bp inverted repeat sequence located between -65 and -43 of the catApl promoter. We roamed this sequence HRE (H$_2$O$_2$-responsive Element). The distal half of the inverted repeat was more crucial for H$_2$O$_2$-dependent induction of the catApl transcript than the proximal half. HRE most likely serves as a binding site for the H$_2$O$_2$-responsive repressor CatR.

• 한국자원식물학회지
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• 제36권3호
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• pp.225-236
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• 2023

Stomatal traits such as stomata density (SD), aperture length (APL) and width (APW), guard cell length (GCL) and width (GCW), and distance between stomata (DIS) were investigated to identify correlation with agronomic traits for 35 Korean wheat cultivars. Flag leaf width (FLW) of Korean wheat cultivars was the widest in Ol-mil, and the narrowest in Keumkang. SD tended to be higher on the adaxial side than on the abaxial side in Korean wheat cultivars. SD of adaxial and abaxial sides was classified into a cultivar with a significantly different or not. In APL, 18 wheat cultivars showed significant differences according to leaf side, and APL of adaxial was longer than APL of abaxial in 13 wheat cultivars. In APW, 15 wheat cultivars showed a significant difference, and APW of abaxial was wider than APW of adaxial among them. In GCL, 14 wheat cultivars showed a significant difference, and the GCL of abaxial was longer than the GCL of adaxial in 10 wheat cultivars. In GCW, 10 wheat cultivars showed a significant difference, GCW of adaxial was wider than GCW of abaxial and in 6 wheat cultivars. FLW of adaxial and abaxial showed a negative correlation with GCL and a positive correlation with grain number per panicle. FLW of only abaxial showed a positive correlation with DIS. The SD of the adaxial showed a negative correlation with GCL, while the SD of the abaxial showed a negative correlation with APL. APL of both sides of the leaf showed a positive correlation with GCL, and APW of only abaxial showed a negative correlation with GLC. DIS of adaxial showed a negative correlation with tiller number (TN), while DIS of abaxial showed a positive correlation with GNP.

• 한국우주과학회:학술대회논문집(한국우주과학회보)
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• 한국우주과학회 2008년도 한국우주과학회보 제17권1호
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• pp.22.4-23
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• 2008

• 한국멀티미디어학회논문지
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• 제13권9호
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• pp.1299-1313
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• 2010

Top-k 질의는 데이터베이스에서 사용자가 가장 원하는 k개의 객체를 구하는 질의이다. Top-k 질의를 효율적으로 처리하는 대표적인 연구로 Partitioned-Layer Index (간단히, PL-index) 방법이 있다. PL-index는 데이터베이스를 여러 개의 더 작은 데이터베이스로 분할하고 각 분할된 데이터베이스에 대해 sublayer들의 list (간단히, sublayer list)를 구성한다. 이때, 분할된 데이터베이스에 대해서 top-i 결과가 될 수 있는 객체들을 그 분할된 데이터베이스에 대한 i번째 sublayer로 구성한다. 그리고 주어진 질의에 맞춰 그 sublayer list들을 병합함으로써 질의 결과를 구한다. PL-index는 질의 처리 시 데이터베이스로부터 읽어 들이는 객체의 개수가 매우 작다는 장점을 가지지만, sublayer list들을 병합할 때에 임의 접근(random access)이 많이 발생하기 때문에 디스크 기반의 데이터베이스 환경에서 질의 처리 성능이 저하된다. 이에 본 논문에서는 임의 접근 횟수를 줄임으로써 디스크 기반의 데이터베이스 환경에서 PL-index의 질의 처리 성능을 크게 향상시키는 요약된(Abstracted) Partitioned-Layer Index (간단히, APL一index)를 제안한다. 먼저, PL-index의 각 sublayer를 가상의 (점) 객체로 요약함으로써 sublayer list들을 이러한 점 객체들의 list들(즉, APL-index)로 변형한다. 그리고 APL-index에 대해 질의 처리를 가상으로 수행하여 실제 질의 처리 시 접근할 sublayer를 예측한다, 그리고 예측된 sublayer들을 sublayer list별로 한꺼번에 읽어 들임으로 PL-index에서 발생하는 임의 접근 횟수를 줄인다. 합성 데이터와 실제 데이터에 대한 실험을 통하여 제안한 APL-index가 PL-index의 임의 접근 횟수를 크게 줄일 수 있음을 보인다.