• Title/Summary/Keyword: ALS activity

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Inhibition Characteristics of Chlorsulfuron and Imazaquin on Acetolactate Synthase Activity of Corn Plants (Chlorsulfuron 및 Imazaquin에 의한 옥수수 Acetolactate Synthase 활성의 저해특성)

  • Hwang, I.T.;Kim, K.J.;Lee, H.J.;Cho, K.Y.;Chun, J.C.
    • Korean Journal of Weed Science
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    • v.16 no.2
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    • pp.122-131
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    • 1996
  • The inhibition characteristics of chlorsulfuron [CHL, 2-chloro-N-[{ (4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino}carbonyl]benzenesulfonamide] and imazaquin [IMA, 2-{4,5-dihydro-4-methyl-4-(1-methy-lethyl)-5-oxo-1H-imidazol-2-yl}-3-quinolinecarboxylic acid] on acetolactate synthase(ALS) activity of corn plants were investigated. CHL and IMA rapidly inhibited ALS activity of corn plants in vitro. Their $I_{50}$ values for ALS activity were 100nM and $5{\mu}M$, respectively, indicating that CHL had 50 times more inhibitory effect on ALS activity than IMA. The first applied herbicide had a dominant inhibitory effect on ALS activity when the two herbicides were applied sequentially. Branched-chain amino acids, valine(Val), leucine(Leu), and isoleucine(Ile) showed a feedback inhibition on ALS activity ; Val or Leu had a more inhibitory effect on ALS activity than Ile. Branchedchain amino acids and CHL or IMA exhibited an additive effect on inhibiting ALS activity. This suggests that branched-chain amino acids inhibit ALS activity by a different mechanisms) from that of CHL or IMA. Apparent ALS activity, which was measured on the basis of the conversion of pyruvate to acetolactate, was decreased by the addition of 2-ketobutyrate into the ALS reaction mixture in a concentration-dependent manner. In addition, kinetic studies revealed that CHL acts as a noncompetitive inhibitor, while IMA acts as an uncompetitive inhibitor to ALS with respect to pyruvate.

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Response and Acetolactate Synthase Activity in Different Rice Cultivars(Oryza sativa L.) to Cinosulfuron (Cinosulfuron에 대한 벼 품종간의 생육반응과 Acetolactate Synthase 활성에 미친 영향)

  • Park, Sang-Jo;Kim, Kil-Ung;Shin, Dong-Hyun
    • Korean Journal of Weed Science
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    • v.16 no.2
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    • pp.132-139
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    • 1996
  • Acetolactate synthase(ALS) activity was determined in germinating seedlings of two rice cultivars treated with cinosulfuron [3-(4,6-dimethoxy-1,3,5-triazin-2-yl)-1-[2-methoxyethoxy)-phenylsulfonyl]-urea]. IR 74(Indica type) was more tolerant than Hwajinbyeo(Japonica type) under various rates of cinosulfuron applied at the pregermination stage. In vitro response of ALS activity in the two rice cultivars was similar to $I_{50}$ values(cinosulfuron concentration required for 50% inhibition of ALS activity) of about 23ppb. In vivo, ALS activity of IR 74 increased as the seedlings grew, but that of Hwajinbyeo dropped at 5 days after 10ppm cinosulfuron treatment and shoot growth of Hwajinbyeo lagged at 4 to 5 days after herbicide treatment. ALS activity and shoot growth of Hwajinbyeo was resumed from cinosulfuron-induced inhibition at 6 days after cinosulfuron treatment. The differential response of ALS activity in two different rice cultivars against cinosulfuron may not be due to difference of ALS sensitivity, but rather due to different metabolic inactivation rates of cinosulfuron.

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The Effects of Branched Chain Amino Acids and Small Metabolites on the Biosynthesis of Acetolactate Synthase in Serratia rnarcescens ATCC 25419 (Branched Chain 아미노산과 대사산물들이 Serratia marcescens ATCC 25419 Acetolactate Synthase의 생합성에 미치는 영향)

  • 최병범;김승수
    • Microbiology and Biotechnology Letters
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    • v.20 no.2
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    • pp.115-121
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    • 1992
  • The effects of branched chain amino acids and small metabolites in growth media on the biosynthesis of Serratia marcescens ATCC 25419 acetolactate synthase (ALS) were examined. ALS activ~ty was gradually decreased by isoleucme or leucine among the range from 1 mM to 20 rnM, while the activity was increased 40% by isoleucine under low concentration (0.5 mM). ALS activity was also increased about 40% by valine among 2 to 4 mM ranges, but the activity was decreased only 10% at 20 mM. ALS activity was decreased 25% and 70% by the simultaneous addition of all three branched chain amino acids at 2 mM and 10 mM, respectively. Among several small metabolites tested, ALS activity was increased about 2-fold by cAMP at 2 mM. These data suggest that Sorrtrtiti rnorcewns ALS is muitivalently repressed by branched chain amino acids, but not repressed by valine alone.

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Effect of Pyrimidylsalicylate on the Valine Sensitive Acetolactate Synthase Purified from Serroatia marcescens

  • Yang, Jeong-Hee;Kim, Soung-Soo
    • BMB Reports
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    • v.30 no.1
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    • pp.13-17
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    • 1997
  • The inhibitory effect of herbicides such as sulfonylurea derivatives, imidazolinones and pyrimidylsalicylate has been examined on the purified valine sensitive acetolactate synthase (ALS) from Serratia marcescens. The concentration of sulfometuron methyl which inhibits 50% of the ALS activity was 2.5 mM. The required concentrations of triasulfuron, primisulfuron methyl and imazaquin for the 50% inhibition of the ALS activity were 1 mM. The resistance of Serratia ALS to sulfometuron methyl, imazapyr and imazaquin is similar to that of E. coli ALS 1. However, pyrimidylsalicylate showed a potent inhibitory effect on the Serratia ALS almost 13 times more potent than on E. coli ALS II, which is known as herbicide-sensitive isozyme. The inhibitory mode was competitive against pyruvate. 150 value was determined to be $17{\mu}M$ in an assay mixture containing 20 mM pyruvate, and the $K_1$, value was calculated to be $0.4{\mu}m$ from the modified double reciprocal plot of 1/V versus $1/S^2$.

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Pulseless electrical activity during general anesthesia induction in patients with amyotrophic lateral sclerosis

  • You, Tae Min;Kim, Seungoh
    • Journal of Dental Anesthesia and Pain Medicine
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    • v.17 no.3
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    • pp.235-240
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    • 2017
  • Pulseless electrical activity (PEA) is a clinical condition characterized by unresponsiveness and lack of palpable pulse in the presence of organized cardiac electrical activity and is caused by a profound cardiovascular insult (e.g., severe prolonged hypoxia or acidosis, extreme hypovolemia, or flow-restricting pulmonary embolus). Amyotrophic lateral sclerosis (ALS) is a disease that is characterized by progressive degeneration of all levels of the motor nervous system. Damage to the respiratory system and weakness of the muscles may increase the likelihood of an emergency situation occurring in patients with ALS while under general anesthesia. We report a case of PEA during the induction of general anesthesia in a patient with ALS who presented for dental treatment and discuss the causes of PEA and necessary considerations for general anesthesia in patients with ALS.

Physical Function of Patients with Amyotrophic Lateral Sclerosis (근위축성측삭경화증 환자의 신체적 기능 상태)

  • Lee, Yoon-Kyoung;Lim, Nan-Young;Kim, Seung-Hyun
    • Journal of muscle and joint health
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    • v.13 no.2
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    • pp.130-139
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    • 2006
  • Purpose: This study was designed to present preliminary data for the development of appropriate nursing care system for the patients with ALS by analyzing their physical function. Method: The clinical data of 36 ALS patients, who visited ALS Clinic of H University Hospital in Seoul, were collected from January, 2006 to August, 2006. To determine the physical function, Norris ALS scale and Appel ALS Rating Scale were used. The data were analyzed by frequency, percentage, mean, standard deviation, range, t-test, ANOVA, using SPSS PC program. Results: The mean score of physical activity, muscle strength, upper extremity function, lower extremity function was 18.08, 27.72, 25.94, 25.19 respectively. There were significant differences in physical activity, muscle strength, and upper extremity function according to sender and comorbid disease(diabetes). Although sites of symptom onset were not statistically significant with all physical function, patients with bulbar onset showed relatively severe physical disabilities. Conclusion: The preliminary data on physical function of patients with ALS would be helpful for the development of ALS nursing guideline system.

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Separation and Characterization of Two Forms of Acetolactate Synthase from Etiolated Pea Seedlings

  • Shin, Yong-Soo;Chong, Chom-Kyu;Choi, Jung-Do
    • BMB Reports
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    • v.32 no.4
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    • pp.393-398
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    • 1999
  • Acetolactate synthase (ALS) catalyzes the first reaction common to the biosynthesis of L-valine, L-leucine, and L-isoleucine. ALS is the target site of several classes of herbicides, including the sulfonylureas, the imidazolinones, and the triazolopyrimidines. Two forms of ALS (ALS I and ALS II) which have different affinity for Heparin have been separated from etiolated pea seedlings. The substrate saturation curves of both ALS I and ALS II were hyperbolic in contrast to previous reports. The two forms of ALS showed significant differences in their physical and kinetic properties. The values of $K_m$ for ALS I and ALS II were 9.0 mM and 4.8 mM, respectively. The pI values for ALS I and ALS II were determined to be 5.3 and 5.75 by isoelectric focusing, respectively. The native molecular weights for ALS I and ALS II obtained by nondenaturing gel electrophoresis and activity staining were 124 and 244 kDa, respectively. They also exhibited different sensitivity to feedback inhibition by end-product amino acids and inhibition by Cadre, an imidazolinone herbicide.

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Purification and Characterization of Acetolactate Synthase from Barley

  • Chong, Chom-Kyu;Chang, Soo-Ik;Choi, Jung-Do
    • BMB Reports
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    • v.30 no.4
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    • pp.274-279
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    • 1997
  • Acetolactate synthase (ALS) catalyzes the first common step in the biosynthesis of branchedchain amino acids, valine, leucine, and isoleucine. ALS is the target site for several structually diverse classes of herbicides including sulfonylureas, imidazolinones. and triazolopyrimidines. We have purified ALS from etiolated barley shoots to homogeneity. The five major purification steps are ammonium sulfate fractionation, DEAE anion exchange, hydroxylapatite, Bio-Gel A gel filtration, and low pressure Mono-Q chrornatoqraphy. Approximately 170-fold purification was achieved and the yield was 0.45% of initial activity in the crude extract. Both SDS-PAGE and Western blot analysis showed a single polypeptide of ALS with an apparent molecular mass of 64 kDa. The result of nondenaturing gel electrophoresis with activity staining indicated that the molecular mass of its native form is approximately 225 to 250 kDa. The values of $K_m$ for pyruvate. pl. and optimum pH of ALS were determined to be 2.0 mM, 5.2. and 7.0. respectively Feedback inhibition studies showed that ALS is more susceptible to leucine than valine. And $IC_{50}$ value of Cadre, a class of irnidazolinones, is about $1.5\mu{M}$ for ALS.

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An Active Site Arginine Residue in Tobacco Acetolactate Synthase

  • Kim, Sung-Ho;Park, En-Joung;Yoon, Sung-Sook;Choi, Jung-Do
    • Bulletin of the Korean Chemical Society
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    • v.24 no.12
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    • pp.1799-1804
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    • 2003
  • Acetolatate synthase(ALS) catalyzes the first common step in the biosynthesis of valine, leucine, isoleucine in plants and microorganisms. ALS is the target of several classes of herbicides, including the sulfonylureas, the imidazolinones, and the triazolopyrimidines. To elucidate the roles of arginine residues in tobacco ALS, chemical modification and site-directed mutagenesis were performed. Recombinant tobacco ALS was expressed in E. coli and purified to homogeneity. The ALS was inactivated by arginine specific reagents, phenylglyoxal and 2,3-butanedione. The rate of inactivation was a function of the concentration of modifier. The inactivation by butanedione was enhanced by borate, and the inactivation was reversible on removal of excess butanedione and borate. The substrate pyruvate and competitive inhibitors fluoropyruvate and phenylpyruvate protected the enzyme against inactivation by both modifiers. The mutation of well-conserved Arg198 of the ALS by Gln abolished the enzymatic activity as well as the binding affinity for cofactor FAD. However, the mutation of R198K did not affect significantly the binding of FAD to the enzyme. Taken together, the results imply that Arg198 is essential for the catalytic activity of the ALS and involved in the binding of FAD, and that the positive charge of the Arg is crucial for the interaction with negatively charged FAD.

Characterization of Two Forms of Acetolactate Synthase from Barley

  • Yoon, Jong-Mo;Yoon, Moon-Young;Kim, Young-Tae;Choi, Jung-Do
    • BMB Reports
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    • v.36 no.5
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    • pp.456-461
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    • 2003
  • Acetolactate synthase (ALS) catalyzes the first common step in the biosynthesis of valine, leucine, and isoleucine. ALS is the target site for several classes of herbicides, including sulfonylureas, imidazolinones, and triazolopyrimidines. Two forms of ALS (designated ALS I and ALS II) were separated from barley shoots by heparin affinity column chromatography. The molecular masses of native ALS I and ALS II were determined to be 248 kDa and 238 kDa by nondenaturing gel electrophoresis and activity staining. Similar molecular masses of two forms of ALS were confirmed by a Western blot analysis. SDS-PAGE and Western blot analysis showed that the molecular masses of the ALS I and ALS II subunits were identical - 65 kDa. The two ALS forms exhibited different properties with respect to the values of $K_m$, pI and optimum pH, and sensitivity to inhibition by herbicides sulfonylurea and imidazolinone as well as to the feedback regulation by the end-product amino acids Val, Leu, and Ile. These results, therefore, suggest that the two ALS forms are not different polymeric forms of the same enzyme, but isozymes.