• Asian Pacific Journal of Cancer Prevention
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• v.14 no.5
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• pp.3139-3142
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• 2013

Associations of P16, MGMT, hMLH1 and hMLH2 with gastric cancer and their relation with MTHFR status in gastric patients who were confirmed with pathological diagnosis were assessed. Aberrant DNA methylation of P16, MGMT, hMLH1 and hMLH2 and polymorphisms of MTHFR C677T were assayed. The proportional DNA hypermethylation in P16, MGMT, hMLH1 and hMLH2 in cancer tissues was significantly higher than in remote normal-appearing tissues. DNA hypermethylation of P16 and MGMT was correlated with the T and N stages. Individuals with homozygotes (TT) of MTHFR C677T had significant risk of hypermethylation of MGMT in cancer tissues [OR (95% CI)= 3.47(1.41-7.93)]. However, we did not find association between polymorphism in MTHFR C677T and risk of hypermethylation in P16, MGMT, hMLH1 and hMLH2 genes either in cancer or remote normal-appearing tissues. Aberrant hypermethylation of P16, MGMT, hMLH1 and hMLH2 could be predictive of gastric cancer.

• Korean Journal of Poultry Science
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• v.46 no.4
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• pp.235-247
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• 2019

Serological monitoring has been conducted worldwide for early diagnosis of disease and monitoring of immune status in poultry. This study was conducted to evaluate the immune status of layers with sera submitted to the Avian Disease Laboratory, Chungbuk National University from 2015 to 2017. The test results were analyzed by the time submitted and by the age of the chicks. Low pathogenic avian influenza (LPAI) showed a low positive rate of antibody compared with those of Newcastle disease, indicating that domestic vaccination against LPAI was not sufficient. The antibody profile of infectious bronchitis (IB) depicted high level of titer and a low tendency of CV as compared to the uninfected control flocks, which indicated that most layer farms have been exposed to the field IB virus. In case of avian metapneumovirus infection (aMPV) and Mycoplasma synoviae (MS), since the introduction of the vaccine in 2011 and 2017, respectively, the positive rate and the titer level were higher than those in pevious times. No significant difference in the changes of seasonal result was observed, indicating proper vaccination and improvement in biosecurity and management.

• Parasites, Hosts and Diseases
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• v.47 no.3
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• pp.227-233
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• 2009

Local malaria transmission in the United Arab Emirates (UAE) came to an end in 1997. Nevertheless, UAE has been subjected to substantial importation of malaria cases from abroad, concerning both UAE nationals and immigrants from malarious countries with a total number of 2,119 cases in 2007. To evaluate a new DNA extraction technique using nested PCR, blood samples were collected from 132 individuals who presented to Infectious Diseases Department in Rashid Hospital, Dubai, and Central Department of Malaria Control with fever and persistent headache. Giemsa-stained blood films and ELISA test for malaria antibodies were carried out for detection of Plasmodium infection. Plasmodium infections were identified with the genus-specific primer set and species differentiation using nested PCR. A rapid procedure for diagnosis of malaria infections directly from dried blood spots using for the first time DNA extract from FTA Elute cards was evaluated in contrast to extraction techniques using FTA classic cards and rapid boiling technique. Our new simple technique for DNA extraction using FTA Elute cards was very sensitive giving a sensitivity of 100% compared to 94% using FTA classic cards and 62% in the rapid boiling technique. No complex preparation of blood samples was required prior to the amplification. The production cost of DNA isolation in our PCR assay was much less incomparable to that of other DNA extraction protocols. The nested PCR detected plasmodial infection and could differentiate P. falciparum from P. vivax, and also detected the mixed infection.

• Korean Journal of Ecology and Environment
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• v.40 no.4
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• pp.527-536
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• 2007

In this study, we applied 10-metric health assessment model, based on the Index of Biological Integrity (IBI) during 2006 in the Changwon Stream, which is located in the Changwon city, Gyeongnam province, S. Korea, and then compared with water quality data. The Index of Biological Integrity (IBI) in the Changwon Stream varied from 18 to 38 in the watershed depending on the sampling location and averaged 30.3 (n=6) during the study. Analysis of tolerance guilds showed that the proportion of sensitive species was 13%, but tolerant and intermediate species were 34% and 53%, respectively. Qualitative Habitat Evaluation Index (QHEI) averaged 43.3 (range: 65-104, n=6) indicating non-supporting condition, based on the criteria of U.S. EPA (1993). Values of QHEI showed a typical longitudinal decreases from the headwater reach to the downstream location, except for Site 1 with a low QHEI value by artificial habitat by concrete construction. Minimum QHEI was found in Site 4 where fish diversity was minimal. Conductivity increased continuously along the gradients and especially showed abrupt increases in the downstream sites along with turbidity. Stream ecosystem health of IBI matched to the values of QHEI except for S6. Low IBI values in the sites 4 and 5 was considered to be a result of combined effects of chemical pollutions and habitat degradations. Our results support the hypotheses of Plafkin et ai. (1989) that physical habitat quality directly influences the trophic structure and species richness, and is closely associated with IBI values.

• Clinical and Experimental Pediatrics
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• v.62 no.5
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• pp.187-192
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• 2019

Purpose: The importance of the neurodevelopmental outcomes of very-low-birth-weight (VLBW) infants has been emphasized as their mortality rate has markedly improved. This study aimed to assess the validity of the Korean Developmental Screening Test (K-DST), a developmental screening tool approved by the Korean Society of Pediatrics, for the timely diagnosis of neurodevelopmental delay in VLBW infants. Methods: Subjects included VLBW infants enrolled in the Korean Neonatal Network database between January 2012 and December 2014. The collected data were analyzed for sensitivity, specificity, positive predictive value, and negative predictive value (NPV) in the K-DST compared to those in the Bayley Scales of Infant Development-II for VLBW infants. Results: A total of 173 patients were enrolled. Their mean gestational age and mean birth weight were $27.5{\pm}2.8weeks$ and $980.5{\pm}272.1g$, respectively. The frequency of failed psychomotor developmental index (PDI) <85 was similar to that in at least one domain of K-DST <1 standard deviation. Failure in more than one K-DST domain compared with a mental developmental index (MDI) <85 showed a sensitivity and NPV of 73.2% and 75.0%, respectively. Failure in more than one K-DST domain compared with PDI <85 showed a sensitivity and NPV of 60.3% and 71.6%, respectively. Each K-DST domain had a stronger correlation with predicting a failing MDI <85 than a failing PDI <85 (P<0.05). Conclusion: K-DST could be a useful screening tool for predicting mental developmental delay in VLBW infants and referring them for neurodevelopmental assessments.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.7
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• pp.3287-3292
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• 2012

Purpose: To study the overall treatment time (OTT) and acute toxicity of intensity-modulated radiotherapy (IMRT) treatment for nasopharyngeal carcinoma (NPC). Methods: This retrospective study covered all NPC patients who underwent radical IMRT treatment at the Penang General Hospital from June 2011 to February 2012. Patients of any age and stage of disease with histologically proven diagnosis were included. Information was collected on patient demographics, clinical stage, treatment received, including any neoadjuvant and/or concurrent chemotherapy, acute toxity and completion of IMRT within the OTT. Results: A total of 26 NPC patients were treated with IMRT during the study period; 88.5% had stage III/IV disease. 45.2% received neo-adjuvant chemotherapy while 50.0% were given concurrent chemo-irradiation. All patients completed the treatment and 92.3% within the 7 weeks OTT. Xerostomia was present in all patients with 92.3% having grade 2. Severe grade III/IV acute toxicity occurred in 73.1% of patients, the commonest of which was oral mucositis (57.6%). This was followed by dysphagia which occurred in 53.8%, skin reactions in 42.3% and weight loss in 19.2%. However, haematological toxicity was mild with only one patient having leucopaenia. Conclusion: IMRT treatment for NPC is feasible in our center. More importantly, it can be delivered within the 7 weeks OTT in the majority of patients. Severe grade 3/4 toxicity is very common (73.1%) and thus maximal nutritional and analgesic support is required throughout the treatment.

• Journal of Society of Preventive Korean Medicine
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• v.28 no.1
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• pp.31-42
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• 2024

Objective : In order to understand the changes in domestic approval regulations applicable to traditional Korean medical device companies, this article will explain the major amendments 「Regulation on Medical Device Approval Report Review, etc.」 from 2005 to the present on a year-by-year basis, and provide a counter plan to the recent changes in approval regulations. Methods : We analysed the changes in approval regulatory amendments related to the traditional Korean medical devices from 2005 to the present. Results : The Ministry of Food and Drug Safety is continuously improving medical device approval regulations to ensure the global competitiveness of domestic medical devices and contribute to the improvement of public health. Recent major approval regulatory amendments include the establishment of a review system for software medical devices and digital therapeutics, the recognition of real world evidence materials, the introduction of a biological evaluation of medical devices within a risk management process and a medical device approval licence renewal system. Conclusions : It is expected that the range of medical devices available to Korean medicine doctors will continue to expand in the future through the provision of non-face-to-face medical services and the development of advanced and new medical devices, as well as wearable medical devices and digital therapeutics. In order to increase the market entry potential of traditional Korean medical devices that incorporate advanced technologies such as digital technology and AI-based diagnosis and prediction technology, it is urgent that the government provide significant support to traditional Korean medical device companies to improve approval regulatory compliance.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.18
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• pp.8145-8147
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• 2016

Background: Atypical squamous cells of undetermined significance (ASCUS) feature a wide variety of cervical cells, including benign and malignant examples. The management of ASCUS is complicated. Guidelines for office gynecology in Japan recommend performing a high-risk human papillomavirus (HPV) test as a rule. The guidelines also recommend repeat cervical cytology after 6 and 12 months, or immediate colposcopy. The purpose of this study was to determine the clinical significance of ASCUS. Materials and Methods: Between January 2012 and December 2014, a total of 162 patients underwent cervical conization for cervical intraepithelial neoplasia grade 3 (CIN3), carcinoma in situ, squamous cell carcinoma, microinvasive squamous cell carcinoma, and adenocarcinoma in situ at our hospital. The results of cervical cytology prior to conization, the pathology after conization, and high-risk HPV testing were obtained from clinical records and analyzed retrospectively. Results: Based on cervical cytology, 31 (19.1%) of 162 patients were primarily diagnosed with ASCUS. Among these, 25 (80.6%) were positive for high-risk HPV, and the test results of the remaining 6 patients (19.4%) were uncertain. In the final pathological diagnosis after conization, 27 (87.1%) and 4 patients (12.9%) were diagnosed with CIN3 and carcinoma in situ, respectively. Conclusions: Although ASCUS is known as a low-risk abnormal cervical cytology, approximately 20% of patients who underwent cervical conization had ASCUS. The relationship between the cervical cytology of ASCUS and the final pathological results for CIN3 or invasive carcinoma should be investigated statistically. In cases of ASCUS, we recommend HPV tests or colposcopic examination rather than cytological follow-up, because of the risk of missing CIN3 or more advanced disease.

• The Journal of the Society of Korean Medicine Diagnostics
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• v.12 no.2
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• pp.1-7
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• 2008

Objectives: The basis research of aged people's healthcare technology is very important, because our nation goes into an aged society rapidly. Especially in traditional medicine, pulse shapes of aged people were supposed to weak and empty pulse until now, but any objective measurement and compare research do not taken. Methods and Results: Therefore in this study we design observation study to find characteristic of pulse between younger group (age $20{\sim}30$) and elder group (age $43{\sim}80$) in Busan area. We used 3-dimensional pulse analyzer '3-D MAC' (Daeyomedi Co., Ltd.). And major parameters for comparison are 1) pulse height (h1), 2) applied pressure, 3) pulse power (energy), 4) high pressure duration (w) and 5) R-AI. Conclusions: According to analysis result, elder people's pulse has more large value in pulse height, applied pressure and pulse power than younger's that. It reveals that elder people in modernized lifestyle has Yang symptoms as well Yin symptoms. Consequently we need new method to care elder people's health, and this means more important direction than old text book. From now on verification research with objective diagnosis device like 3-D MAC, must be activated to build of evidence in traditional medicine. Cause traditional medicine's spirit of care and prevention is suitable to care elder people's cardiac and cerebral vascular disease those are have close relation with elder people.

• Advanced Industrial SCIence
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• v.3 no.1
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• pp.17-22
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• 2024

History, which began with the emergence of mankind, has a means of recording. Today, we can check the past through data. Generated data may only be generated and stored at a certain moment, but it is not only continuously generated over a certain time interval from the past to the present, but also occurs in the future, so making predictions using it is an important task. In order to find out trends in the use of time series data among numerous data, this paper analyzes the concept of time series data, analyzes Recurrent Neural Network and Long-Short Term Memory, which are mainly used for time series data analysis in the machine learning field, and analyzes the use of these models. Through case studies, it was confirmed that it is being used in various fields such as medical diagnosis, stock price analysis, and climate prediction, and is showing high predictive results. Based on this, we will explore ways to utilize it in the future.