Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Imported Malaria in United Arab Emirates: Evaluation of a New DNA Extraction Technique Using Nested PCR

  • Sultan, Doaa M. (Parasitology Department, Mansoura Medical College, Mansoura University) ;
  • Khalil, Marwa M. (Community Medicine Department, Zagazig Medical College, Zagazig University) ;
  • Abdouh, Ahmed S. (Internal Medicine and Infectious Disease Department, Rashid Hospital) ;
  • Doleh, Wafaa F. (Internal Medicine and Infectious Disease Department, Rashid Hospital) ;
  • AI Muthanna, Abdul Aziz M. (Central Department of Malaria Control)
  • Published : 2009.09.30
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Abstract

Local malaria transmission in the United Arab Emirates (UAE) came to an end in 1997. Nevertheless, UAE has been subjected to substantial importation of malaria cases from abroad, concerning both UAE nationals and immigrants from malarious countries with a total number of 2,119 cases in 2007. To evaluate a new DNA extraction technique using nested PCR, blood samples were collected from 132 individuals who presented to Infectious Diseases Department in Rashid Hospital, Dubai, and Central Department of Malaria Control with fever and persistent headache. Giemsa-stained blood films and ELISA test for malaria antibodies were carried out for detection of Plasmodium infection. Plasmodium infections were identified with the genus-specific primer set and species differentiation using nested PCR. A rapid procedure for diagnosis of malaria infections directly from dried blood spots using for the first time DNA extract from FTA Elute cards was evaluated in contrast to extraction techniques using FTA classic cards and rapid boiling technique. Our new simple technique for DNA extraction using FTA Elute cards was very sensitive giving a sensitivity of 100% compared to 94% using FTA classic cards and 62% in the rapid boiling technique. No complex preparation of blood samples was required prior to the amplification. The production cost of DNA isolation in our PCR assay was much less incomparable to that of other DNA extraction protocols. The nested PCR detected plasmodial infection and could differentiate P. falciparum from P. vivax, and also detected the mixed infection.

Keywords

References

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