• Journal of Microbiology and Biotechnology
• /
• 제13권6호
• /
• pp.976-983
• /
• 2003

Seven Escherichia coli cells defective with aerobic growth were isolated by the insertion of ${\lambda}placMu53$, a hybrid bacteriophage of ${\lambda}$ and Mu, which created a transcriptional fusion to lacZY. These insertion mutant cells were tested on an XG ($5-bromo-4-chloro-3-indolyl-{\beta}-D-galactopyranoside$) medium for anaerobic expression of lacZ by fusion to a promoter. The chromosomal DNA from these strains were digested by EcoRI, and the EcoRI fragments that contained the fused gene and lacZ sequence were identified by Southern hybridization, using lacZ containing plasmid as a probe. The EcoRI fragment from each strain was cloned and sequenced. The sequence data were compared with the GenBank database. The mutated gene of three strains, CYT4, CYT5, and OS11, was found to be identical, and it was nrdAB that encoded ribonucleoside diphosphate reductase. The gene nrdAB was at min 50.5 on the Escherichia coli linkage map and 2,348,084 on the physical map, and is involved in hemAe-related reduction-oxidation reaction. OS6 and OS14 mutant strains had insertion at min 8.3 and the mutated gene was hemB. The hemB encodes 5-aminolevulinate dehydratase or porphobilinogen synthase. The OS3 mutant had insertion in cydB at min 16.6. The cydAB encodes cytochrome d oxidase. In the case of OS1, the fusion was made with sucA, the E1 component of ${\alpha}-ketoglutarate$ dehydrogenase.

• 대기
• /
• 제23권1호
• /
• pp.113-121
• /
• 2013

The objective of this study is to develop and evaluate the system to produce the real-time ensemble drought prediction data. Ensemble drought prediction consists of 3 processes (meteorological outlook using the multi-initial conditions, hydrological analysis and drought index calculation) therefore, more processing time and data is required than that of single member. For ensemble drought prediction, data process time is optimized and hardware of existing system is upgraded. Ensemble drought data is estimated for year 2012 and to evaluate the accuracy of drought prediction data by using ROC (Relative Operating Characteristics) analysis. We obtained 5 ensembles as optimal number and predicted drought condition for every tenth day i.e. 5th, 15th and 25th of each month. The drought indices used are SPI (Standard Precipitation Index), SRI (Standard Runoff Index), SSI (Standard Soil moisture Index). Drought conditions were determined based on results obtained for each ensemble member. Overall the results showed higher accuracy using ensemble members as compared to single. The ROC score of SRI and SSI showed significant improvement in drought period however SPI was higher in the demise period. The proposed ensemble drought prediction system can be contributed to drought forecasting techniques in Korea.

• 한국정보과학회논문지:시스템및이론
• /
• 제34권1호
• /
• pp.10-18
• /
• 2007

휴대용 단말기에서의 동영상 및 3차원 영상을 처리하는 것이 일반화되면서, H.264 및 3차원 그래픽 가속기 데이타를 처리하기 위한 연산량이 크게 증가하고 있다. 본 연구에서는 H.264 인코더의 움직임 추정기 및 디코더의 움직임 보상기와 3차원 그래픽 렌더링 가속기를 재구성 가능하도록 설계하였다. 움직임 추정기는 효율적인 데이타 스캐닝 방법과 DAU, FDVS 알고리즘을 사용하여, JM8.2에 제시된 다중 프레임 움직임 추정보다 연산량을 평균적으로 70% 이상 감소시키면서 화질 열화가 없도록 하였다. 3차원 그래픽 렌더링 가속기는 중심선 트래버셜 알고리즘을 사용하여 병렬 처리하도록 함으로써 처리량을 증가시켰다. 움직임 추정기와 3차원 렌더링 가속기의 메모리를 재구성 가능한 구조로 설계하여, 2.4Mbits (47%)의 메모리를 공유하였으며, 메모리를 8개의 블록으로 분산시켜 사용되지 않는 부분의 전력 소모를 최소화 할 수 있도록 하였다. 또한, 움직임 보상기와 3차원 렌더링 가속기의 픽셀 프로세서를 공유하여 약 7%의 하드웨어면적을 감소 시켰다.

• 대한치과보철학회지
• /
• 제51권3호
• /
• pp.208-213
• /
• 2013

장애인치과는 비장애인과 별차이 없는 경증 장애인에서부터 질환의 진단이 쉽지 않고 전신관리의 필요에 따른 의학적인 전문성이 요구되거나 행동조절 및 치료의 어려움으로 특수기술과 장비를 필요로 하는 장애인까지 다양하다. 본 증례는 자폐성 장애를 갖은 25세 남자 환자로 구강위생관리가 잘 되지 않아 다수의 우식으로 전반적인 치료를 위해 내원하였다. 장애인 환자에 대한 정보 부족으로 인한 치료 계획 수립의 어려움 및 전신마취 하에서 시술의 어려움을 경험하며 장애인 치과치료시 충분한 상담과 기왕력 파악 후 적절한 치료가 필요할 것으로 사료되었다.

• 대한가정학회지
• /
• 제44권6호
• /
• pp.23-34
• /
• 2006

Graffiti has been recognized as an art form since the influence of free, black culture and Neo-Expressionism in the 1980s, though Graffiti remains the subject of controversy both inside and outside the school Modem Art. Jean Michel Basquiat is the most famous Graffiti artist of the '80. He was regarded as the genius and star of American art, leaving a lot of experimental and creative works during his short, 9-year, creative period. In his works, Basquiat well expressed the isolated and dark shadows in the U.S., the pursuit of self identification, the purpose of expression and the epochally social phenomenon. The purpose of this study is to investigate the application of Jean Michel Basquiat's Graffiti to fashion painting. To achieve this purpose, we present a lot of fashion painting works which apply Jean Michel Basquiat's Graffiti by focusing on autographic experience, racialism, cartoon themes, monely value, and anatomical death in the characteristics of Basquiat's works. Through this process, we can express and apply Basquiat's Graffiti to fashion painting by analogizing the themes and modeling the methods of his works, such as childish and simple features, intentionally wrong spellings and sentences, and symbols of death including skeletons, intestines, bones and teeth. In addition, Basquiat's techniques are examined in this study, including the representational handling of a brush, primitive and strong colors, and maximized shape. This study found that fashion painting can juxtaposes art and fashion by expressing Jean Michel Basquiats' Graffiti.

• 복식
• /
• 제54권7호
• /
• pp.55-62
• /
• 2004

Research into the ancient dyeing and weaving culture is of critical importance to the fact that they constitute an integral part of the Korean costume. Due to the absence of the originals reflecting ancient costume cultures, it is difficult to figure out what the dyeing and weaving culture was like in ancient times. Because of limited visual materials available from the wall paintings of ancient tombs, studies conducted so far concerning the costume culture for the Goguryeo have focused on visual elements such as shape, structure, and type. As a result, research into ancient Korean dyeing and weaving cultures hasn't been properly made. Thanks to the presence of both some dyeing fabric originals from Goguryeo's neighboring countries and some visual materials like the wall paintings of the Goguryeo, it may be possible to obtain some clues to the dyeing and weaving culture. The dotted Pattern costumes were selected such as Muyongchong, No. 1 Jangcheon Tomb, Gakjeochong, Samsilchong, Ssangyeongchong, Susanri Tomb. According to a book titled ‘Hanwon’, the Goguryeo people manufactured fabrics in such a way that resist-dyed spots produced elaborate patterns over the purple fabric. It can be safely said that such dot patterns were produced by means of dyeing rather than weaving, because identical patterns aren’t well produced by means of painting and embroidery. Considering Goguryeo’s natural features, the dot patterns mentioned in historical literatures are thought to have been produced using animal’s skin. However, there is highest possibility that the dot Patterns were Produced using tie-resist dyeing or wax-resist dyeing techniques. With respect to the dyeing and weaving culture for the Goguryeo, one must refer to neighboring countries’ dyeing and weaving environments, given that the Goguryeo had engaged substantial cultural exchanges with China.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2017년도 9th Asian Crop Science Association conference
• /
• pp.217-217
• /
• 2017

Seed priming leading to improved emergences, germination speed and uniformity under unfavorable conditions without loss of longevity are the best level of techniques. We studied the possibility to obtain primed seed with reduced mean germination time (MGT) and uniformity but with simple, fast and effective methods. The experiments were carried out at $15^{\circ}C$ for 16 hours with priming treatment. To compare the effects of each treatment, we used 200mM $CaCl_2$ priming or hydro priming or no imbibition after ultrasonication or red light treatment. The light treatment was performed by irradiating 2000 lux of red light for 15, 30, 60, and 120 minutes. Addition, in order to investigate the effect of the priming solution, treated at 200mM $CaCl_2$ for 8 hours. Ultrasonication treatment was performed for 5, 10, and 20 minutes at exposures of 13.0, 17.3, and 21.6 kHz during priming. For liquid smoke (LS) treatment, seed soaked in 0%, 0.5%, 1.0%, 5.0% and 10.0% of diluted water and 200mM $CaCl_2$ solution. After each treatment, the seeds were dried to moisture content ranged 5-8% at $25^{\circ}C$ for 24 hours. The effect of seed treatment was evaluated with germination percentage (GP), MGT, germination index(GI), germination rate(GR), Germination Uniformity(GU) and heath seed percentage(HS). For several factors tested, we found that the desired germination improvement could be obtained by treating the seed with ultrasonication at 17.3 kHz for 5 minutes in water or red light exposure at 2000 lux for 120 min in water that resulted very similar to those used to 200mM $CaCl_2$ priming for 16 hrs. However, LS treatment showed no improvement in all diluted solution. Therefore, the methods applied ultrasonication and red light treatment showed high potential for fast and easy treatment avoiding pollution of salt solution waste.

• Applied Biological Chemistry
• /
• 제39권4호
• /
• pp.260-264
• /
• 1996

Cymbidium속 난의 형질전환계를 확립하기 위해서, microprojectile bombardment 방법을 이용하여 춘란(Cymbidium virescence)의 rhizome 조직세포에 외래 유전자를 도입하는 조건을 검토하였다. 각 parameter별 적정조건으로서 텅스텐 입자의 크기는 $1.11\;{\mu}m$, He 가스 압력은 $77.33kg/cm^2$, gap distance는 6.35mm, target distance는 7.0cm 이었다. DNA피복입자를 투사한 $400{\mu}m$ 두께의 rhizome 절편을 2개월간 배양한 뒤 GUS 활성을 조사한 결과 이 유전자가 발현되는 세포들이 관찰되었다. Kanamycin (100 mg/L)을 첨가한 배지에서 6개월 동안 선택배양을 통해 얻은 rhizome의 DNA를 PCR로 분석한 결과 nptII 유전자의 삽입을 확인할 수 있었다.

• Fisheries and Aquatic Sciences
• /
• 제16권3호
• /
• pp.159-164
• /
• 2013

The mouse bioassay and high performance liquid chromatography (HPLC) post-column oxidation method are different methods of quantifying paralytic shellfish poisoning toxins. In this study, we compared their ability to accurately quantify the toxicity levels in two types of field sample (oysters and mussels) with different toxin profiles for routine regulatory monitoring. A total of 72 samples were analyzed by both methods, 44 of which gave negative results, with readings under the limit of detection of the mouse bioassay ($40{\mu}g/100g$ saxitoxin [STX] eq). In 14 oysters, the major toxin components were gonyautoxin (GTX) 1, -2, -3, -4, -5, decarbamoylgonyautoxin-2 (dcGTX2), and decarbamoylsaxitoxin (dcSTX), while 14 mussels tested positive for dcSTX, GTX2, -3, -4, -5, dcGTX2, neosaxitoxin (NEO), STX, and dcSTX. When the results obtained by both methods were compared in two matrices, a better correlation ($r^2=0.9478$) was obtained for mussels than for oysters ($r^2=0.8244$). Additional studies are therefore needed in oysters to investigate the differences in the results obtained by both methods. Importantly, some samples with toxin levels around the legal limit gave inconsistent results using HPLC-based techniques, which could have a strong economic impact due to enforced harvest area closure. It should therefore be determined if all paralytic shellfish poisoning toxins can be quantified accurately by HPLC, and if the uncertainties of the method lead to doubts regarding regulatory limits.

• 한국환경농학회지
• /
• 제26권2호
• /
• pp.186-191
• /
• 2007

1998년부터 2006년까지 국내 방사능 교차분석에 참여하여 제주지역의 환경방사능 감시를 위한 방사능 분석 기술의 능력 검증과 신뢰도를 확보하기 위하여 수행되었다. 전베타 방사능 분석 시료는 공기부유진 필터와 물이었고, 감마 분석은 토양과 물 시료 중 자연 및 인공 방사성 핵종들이었다. 전베타 방사능 분석 값은 1998년과 1999년 물 시료를 제외하고는 모두 신뢰도 범위내의 값을 가졌고, 감마 핵종은 토양 시료 중의 $^{40}K$와 $137^{CS}$ 그리고 물 시료 중 몇 개의 핵종을 제외하고는 대부분 매우 우수한 평가를 받았다. 따라서 방사선 이상 사고시 원자력 안전을 위한 제주지역의 환경방사능 감시를 위한 신뢰도를 확보하여 자체적으로 환경방사능을 분석할 수 있는 능력을 함양하였다.