• 대한한의학방제학회지
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• 제32권1호
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• pp.63-82
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• 2024

Objective : Saengkankunbi-tang (SKT) is used as a traditional Korean herbal formula for treatment of liver diseases. We investigated the hepatoprotective effects of SKT plus Epimedium koreanum Nakai (EKE) against arachidonic acid (AA) + iron-mediated cytotoxicity in HepG2 cells and carbon tetrachloride (CCl₄)-mediated acute liver damage in mice. Methods : The cyto-protective effects of SKT + EKE were determined by MTT assay, western blot and fluorescence activated cell sorting analysis. In mice, blood biochemistry and western blot were assessed in CCl₄-induced acute hepatic damage. The animal groups included vehicle-treated control, CCl₄, SKT (200 mg/kg/day), EKE (100 mg/kg/day), SKT (200 mg/kg/day) + EKE (100 mg/kg/day) and silymarin (200 mg/kg/day). Results : In HepG2 cells, pretreatment with SKT + EKE significantly suppressed cytotoxicity induced by AA + iron and reduced the expression of proteins related to apoptosis. In addition, pretreatment with SKT + EKE significantly prevented the increase in H₂O₂ production, GSH depletion, and lower mitochondrial membrane potential induced by AA + iron. In CCl₄-induced liver damage mice, the administration of SKT + EKE prevented the liver damage by inhibition of hepatocyte damage and expression of apoptosis proteins in liver. More importantly, in vitro and in vivo assay, SKT + EKE showed significant effect compare with SKT alone or EKE alone in all parameters. Conclusions : These results indicated that SKT + EKE could protect against oxidative stress-induced liver damage, and SKT + EKE is more effective than SKT alone or EKE alone.

• Journal of Veterinary Science
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• 제25권2호
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• pp.32.1-32.14
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• 2024

Background: Aortic arch (AA) branching patterns vary among different mammalian species. Most previous studies have focused on dogs, whereas those on raccoon dogs remain unexplored. Objectives: The objective of this study was to describe the AA branching pattern in raccoon dogs and compare their morphological features with those of other carnivores. Methods: We prepared silicone cast specimens from a total of 36 raccoon dog carcasses via retrograde injection through the abdominal aorta. The brachiocephalic trunk (BCT) branching patterns were classified based on the relationship between the left and right common carotid arteries. The subclavian artery (SB) branching pattern was examined based on the order of the four major branches: the vertebral artery (VT), costocervical trunk (CCT), superficial cervical artery (SC), and internal thoracic artery (IT). Results: In most cases (88.6%), the BCT branched off from the left common carotid artery and terminated in the right common carotid and right subclavian arteries. In the remaining cases (11.4%), the BCT formed a bicarotid trunk. The SB exhibited various branching patterns, with 26 observed types. Based on the branching order of the four major branches, we identified the main branching pattern, in which the VT branched first (98.6%), the CCT branched second (81.9%), the SC branched third (62.5%), and the IT branched fourth (52.8%). Conclusions: The AA branching pattern in raccoon dogs exhibited various branching patterns with both similarities and differences compared to other carnivores.

• Corrosion Science and Technology
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• 제22권2호
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• pp.99-107
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• 2023

Layered silicate clay montmorillonite (MMT) has been used in nanocomposite coating to improve corrosion protection by reinforcing the barrier property. The better dispersion of MMT in the coating produces a higher barrier effect. Pretreatment with MMT could favor the delamination of clay platelets, facilitating MMT dispersion in the coating. In the present work, a montmorillonite/silica (MMT/Si) composite was prepared by the in situ sol-gel method. x-ray diffraction measurements and field-emission scanning electron microscopy observations showed silica crystal formation and increased basal spacing between the MMT platelets. Composite MMT/Si particles were introduced in an epoxy resin to reinforce the corrosion protection of the coating applied on the AA2024 surface. Electrochemical impedance spectroscopy (EIS) was performed to characterize the protective property of the coating. The results demonstrated the high barrier effect of the coating containing 5 wt% of MMT/Si. Adhesion evaluation after a salt spray test exhibited a high adherence to the epoxy coating containing MMT/Si.

• 한국분말재료학회지
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• 제31권1호
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• pp.37-42
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• 2024

Friction stir spot welding (FSSW) is a solid-state joining process and a rapidly growing dissimilar material welding technology for joining metallic alloys in the automotive industry. Welding tool shape and process conditions must be appropriately controlled to obtain high bonding characteristics. In this study, FSSW is performed on dissimilar materials AA5052-H32 aluminum alloy sheet and SPRC440 steel sheet, and the influence of the shape of joining tool and tool insertion depth during joining is investigated. A new intermetallic compound is produced at the aluminum and steel sheets joint. When the insertion depth of the tool is insufficient, the intermetallic compound between the two sheets did not form uniformly. As the insertion depth increased, the intermetallic compound layer become uniform and continuous. The joint specimen shows higher values of tensile shear load as the diameter and insertion depth of the tool increase. This shows that the uniform formation of the intermetallic compound strengthens the bonding force between the joining specimens and increases the tensile shear load.

• Coupled systems mechanics
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• 제7권5호
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• pp.627-634
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• 2018

Laser beam welding is more advantageous compared to conventional methods. Titanium/Aluminium dissimilar alloy thin sheet metals are difficult to weld due to large difference in melting point. The performance of the weldment depends upon interlayer formation and distribution of intermetallics. During welding, aluminium gets lost at the temperature below the melting point of titanium. Therefore, it is needed to improve a new metal joining techniques between these two alloys. The present work is carried for welding TI6AL4V and AA2024 alloy by using Nd:YAG Pulsed laser welding unit. The performance of the butt welded interlayer structures are discussed in detail using hardness test and SEM. Test results reveal that interlayer fracture is caused near aluminium side due to low strength at the weld joint.

• Animal Bioscience
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• 제37권8호
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• pp.1474-1482
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• 2024

Objective: The experiment aimed to determine the standardized ileal digestibility (SID) of crude protein (CP) and amino acids (AA) in 10 brown rice samples fed to pigs, and to construct predictive models for SID of CP and AA based on the physical characteristics and chemical composition of brown rice. Methods: Twenty-two cannulated pigs (initial body weight: 42.0±1.2 kg) were assigned to a replicated 11×3 incomplete Latin square design, including an N-free diet and 10 brown rice diets. Each period included 5 d adaptation and 2 d ileal digesta collection. Chromic oxide was added at 0.3% to all the diets as an indigestible marker for calculating the ileal CP and AA digestibility. Results: The coefficients of variation of all detected indices for physical characteristics and chemical composition, except for bulk weight, dry matter (DM) and gross energy, in 10 brown rice samples were greater than 10%. The SID of CP, lysine (Lys), methionine, threonine (Thr), and tryptophan (Trp) in brown rice was 77.2% (62.6% to 85.5%), 87.5% (80.3% to 94.3%), 89.2% (78.9% to 98.9%), 55.4% (46.1% to 67.6%) and 92.5% (86.3% to 96.3%), respectively. The best prediction equations for the SID of CP, Lys, Thr, and Trp were as following, SID_CP = -664.181+8.484×DM (R² = 0.40), SID_Lys = 53.126+6.031×ether extract (EE)+0.893×thousand-kernel volume (R² = 0.66), SID_Thr = 39.916+7.843×EE (R² = 0.41), and SID_Trp = -361.588+4.891×DM+0.387×total starch (R² = 0.85). Conclusion: Overall, a great variation exists among 10 sources of brown rice, and the thousand-grain volume, DM, EE, and total starch can be used as the key predictors for SID of CP and AA.

• Animal Bioscience
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• 제37권6호
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• pp.1085-1095
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• 2024

Objective: The main objective of this study was to determine available energy and nutritional digestibility of extruded cereals and the effect of extrusion on the nutritional value of feed ingredients, aiming to provide scientific basis for efficient application of extrusion in the diets of growing pigs. Methods: In Exp. 1, 48 crossbred growing pigs (Duroc×Landrace×Yorkshire) with an initial body weight (BW) of 34.6±2.2 kg were selected and fed with eight diets (non-extrusion or extrusion) to determine the digestible energy (DE), metabolizable energy (ME), and nutrients digestibility. Eight diets included extruded grains (barley, wheat, sorghum, or broken rice), while four had unprocessed grains. In Exp. 2, 9 diets were formulated including 4 cereals with extrusion or non-extrusion and a N-free diet. In addition, 9 growing pigs (BW = 22.3±2.8 kg) were fitted with T-cannula in the distal ileum and arranged in a 9×6 Youden square design. Results: Results show that apparent total tract digestibility of gross energy, dry matter, organic meal, ether extract, neutral and acid detergent fiber was not affected by the extrusion process and there was no interaction between cereal type and extrusion treatment on DE, ME. However, the apparent total tract digestibility for crude protein (CP) increased markedly (p<0.05). The standardized ileal digestibility (SID) of all amino acids (AA) except for leucine remarkably increased by extrusion (p<0.05). There was an interaction on the SID of arginine, leucine, isoleucine, methionine, phenylalanine, cystine, and tyrosine in growing pigs between type of grain and extrusion treatment (p<0.05). Conclusion: Extrusion increased the ileal digestibility of CP and most AA in cereals, however, the DE and ME of cereals were not affected in growing pigs.

• 비파괴검사학회지
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• 제27권1호
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• pp.48-57
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• 2007

사용 중인 항공기 구조를 모사하여 리벳구멍 주위에 피로균열이 존재하는 AA2024-T3 박판을 CFRP 복합재 패치로 접합하여 보수한 후 피로균열 성장거동을 음향방출(AE)을 측정하면서 관찰하였다. 패칭의 효과로 균열성장속도의 뚜렷한 감소와 균열이 인접한 리벳 구멍으로 전파되는 시간을 지연시키는 효과가 있음을 확인하였다. 그리고 패칭 후 균열성장에 의해 탐지된 신호와 패치와 알루미늄 사이의 접합계면 분리에 의한 신호의 구별 가능성을 다변량 자료분석 기법인 주성분분석을 통해 알아보았다. 그 결과 균열성장에 따른 AE신호는 계면분리에 따른 AE신호에 비해 중심주파수가 높고 작은 에너지를 가지는 반면, 계면분리 신호는 균열성장 신호에 비해 상승시간이 길고 중심주파수가 상대적으로 낮으며 비교적 큰 에너지를 가진 것으로 나타났다. 따라서 AE신호의 유형인식 방법은 AE 발생원의 위치표정 결과와 결합할 경우 패치 접합으로 보수된 구조에서의 피로균열 성장거동을 예측하는 방법으로 충분히 활용될 수 있다.

• Corrosion Science and Technology
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• 제6권4호
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• pp.206-210
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• 2007

Aerospace aluminum alloys such as Al alloy 2024-T3 and 7075-T6 are subject to localized corrosion due the existence of intermetallics containing Cu, Mg or Zn. Chromate is currently widely used in the aerospace industry as the corrosion inhibitor for these alloys. However, chromate needs to be replaced due to its strong carcinogenicity. In this study, an extensive pigment screening has been performed to find replacements for chromates. Different categories of inhibitors were evaluated by immersion tests, DC polarization tests and other methods. Phosphates, zinc salts, cerium salts, vanadates and benzotriazole were found to be effective inhibitors for AA7075. Among those inhibitors, zinc phosphate was found to be the most effective in our novel, silane-based, one-step aqueous primer system. The performance of this primer is comparable to that of currently used chromate primers in accelerated corrosion tests, while it is completely chromate-free and its VOC is about 80% less than that of current primers. Studies by SEM/EDS showed that the unique structure of the superprimer accounts for the strong anti-corrosion performance of the zinc phosphate pigment. The self-assembled stratified double-layer structure of the superprimer is characterized by a less-penetrable hydrophobic layer at the top and a hydrophilic layer accommodating the inhibitors underneath. The top layer functions as the physical barrier against water ingress, while the lower layer functions as a reservoirfor the inhibitor, which is leached out only if the coating is damaged by a scratch or scribe. The presence of a silane in the primer further improves the adhesion and anti-corrosion performance of the primer.

• 비파괴검사학회지
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• 제27권1호
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• pp.38-47
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• 2007

항공기 구조를 모사하여 일련의 리벳구멍을 갖는 AA2024-T3 박판 구조를 대상으로 음향방출(AE)을 이용하여 피로균열을 탐지하고, 표면초음파(SAW)를 이용하여 균열길이를 측정하였다. 누적 AE 발생수 곡선은 단균열(short crack)의 발생과 성장에 따라 일정한 간격을 두고 급격히 증가하는 계단식 형태로 나타났으나 피로균열의 성장은 지수함수적인 증가를 보였다. SAW를 이용한 균열길이 측정은 균열길이가 다른 13개의 균열에 대하여 실시하였고, 측정된 데이터를 이동식 현미경으로 측정한 결과와 비교하였다. 그 결과 1 mm 이하의 단균열의 크기를 표면초음파 방법으로 측정하는 것은 거의 불가능하였으나, 1 mm 이상의 균열에서는 비교적 높은 신뢰도로 균열길이 측정이 가능함을 확인함으로써 실용적인 측면에서 이 방법이 유효하게 사용될 수 있는 영역이 존재함을 알 수 있었다.