• Title/Summary/Keyword: A35

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Long-term Usability Evaluation of Low Fish Meal Extruded Pellet Diet for Juvenile Olive Flounder Paralichthys olivaceus at Jeju Fish Farm (제주도 양식장 내 치어기 넙치(Paralichthys olivaceus)의 저어분 EP (Extruded Pellet) 사료 장기간 이용성 평가)

  • Hyunwoon Lim;Jaesik Kim;Daehyun Ko;Jin-Woo Song;Seunghan Lee;Sang-Woo Hur;Kang-Woong Kim;Kyeong-Jun Lee
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.57 no.1
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    • pp.23-31
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    • 2024
  • This study evaluated the utilization of a low fish meal (LFM) diet and black soldier fly (BSF) Hermetia illucens meal and oil as a fish meal (FM) substitute or functional additive for juvenile olive flounder Paralichthys olivaceus at the Jeju fish farm. Two experimental diets replaced FM using animal (tankage, poultry byproduct and tuna byproduct meal) and plant (wheat gluten and soy protein concentrate) protein sources, containing 45% (FM45) and 35% (FM35) of FM, respectively. One experimental diet replaced FM with animal, plant, and BSF meal, fish oil using insect meal and oil (FM35+). After the feeding trial ended, no differences in growth performance, feed utilization, survival and biological indices were observed among all experimental groups. Aspartate aminotransferase and cholesterol levels in the FM35 and FM35+ groups were significantly higher than that in the FM70 group. The linoleic acid level in the muscle was significantly higher in the fish fed with the FM70 diet than in those fed with the FM45, FM35, and FM35+ diets. Thus, the LFM diet is suitable for juvenile olive flounder farming during six months.

ON STABILITY OF EXPANSIVE INDUCED HOMEOMORPHISMS ON HYPERSPACES

  • Koo, Namjip;Lee, Hyunhee
    • Journal of the Chungcheong Mathematical Society
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    • v.35 no.1
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    • pp.77-83
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    • 2022
  • In this paper we investigate the topological stability of induced homeomorphisms on a hyperspace. More precisely, we show that an expansive induced homeomorphism on a hyperspace is topologically stable. We also give examples and a diagram about implications to illustrate our results.

The Brown-Rot Basidiomycete Fomitopsis palustris Has the Endo-Glucanases Capable of Degrading Microcrystalline Cellulose

  • Yoon, Jeong-Jun;Cha, Chang-Jun;Kim, Yeong-Suk;Son, Dong-Won;Kim, Young-Kyoon
    • Journal of Microbiology and Biotechnology
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    • v.17 no.5
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    • pp.800-805
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    • 2007
  • Two endoglucanases with processive cellulase activities, produced from Fomitopsis palustris grown on 2% microcrystalline cellulose(Avicel), were purified to homogeneity by anion-exchange and gel filtration column chromatography systems. SDS-PAGE analysis indicated that the molecular masses of the purified enzymes were 47 kDa and 35 kDa, respectively. The amino acid sequence analysis of the 47-kDa protein(EG47) showed a sequence similarity with fungal glycoside hydrolase family 5 endoglucanase from the white-rot fungus Phanerochaete chrysosporium. N-terminal and internal amino acid sequences of the 35-kDa protein(EG35), however, had no homology with any other glycosylhydrolases, although the enzyme had high specific activity against carboxymethyl cellulose, which is a typical substrate for endoglucanases. The initial rate of Avicel hydrolysis by EG35 was relatively fast for 48 h, and the amount of soluble reducing sugar released after 96 h was $100{\mu}g/ml$. Although EG47 also hydrolyzed Avicel, the hydrolysis rate was lower than that of EG35. Thin layer chromatography analysis of the hydrolysis products released from Avicel indicated that the main product was cellobiose, suggesting that the brown-rot fungus possesses processive EGs capable of degrading crystalline cellulose.

A report of 35 unreported bacterial species in Korea, belonging to the phylum Firmicutes

  • Baek, Min-gyung;Kim, Wonyong;Cha, Chang-Jun;Joh, Kiseong;Kim, Seung-Bum;Kim, Myung Kyum;Seong, Chi-Nam;Yi, Hana
    • Journal of Species Research
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    • v.8 no.4
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    • pp.337-350
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    • 2019
  • In an investigation of indigenous prokaryotic species in Korea, a total of 35 bacterial strains assigned to the phylum Firmicutes were isolated from diverse habitats including natural and artificial environments. Based on their high 16S rRNA gene sequence similarity (>98.7%) and formation of robust phylogenetic clades with species of validly published names, the isolates were identified as 35 species belonging to the orders Bacillales (the family Bacillaceae, Paenibacillaceae, Planococcaceae, and Staphylococcaceae) and Lactobacillales (Aerococcaceae, Enterococcaceae, Lactobacillaceae, Leuconostocaceae, and Streptococcaceae). Since these 35 species in Korean environments has not been reported in any official report, we identified them as unrecorded bacterial species and investigated them taxonomically. The newly found unrecorded species belong to 20 species in the order Bacillales and 15 species in the order Lactobacillales. The morphological, cultural, physiological, and biochemical properties of the isolates were examined and the descriptive information of the 35 previously unrecorded species is provided here.