• 한국응급구조학회지
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• 제14권3호
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• pp.71-81
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• 2010

Purpose: To minimize an interruption in chest compression, reduce the hands-off time, the American Heart Association has recommended the ratio of chest compression to ventilation ratio to 30:2 from 2005 CPR guideline to 2010 CPR guideline. However, current studies have shown that the hands-off time was > 10 seconds with that method. For this reason, we devised new CPR method that a ventilation to chest compression ratio of 2:30 to reduce pt assessment time and skipped the assessment step of carotid artery pulse would be a more effective way to reduce the hands-off time & the time to set the CPR. According to the more detailed purpose are listed below. 1) We would like to confirm efficiency of a ventilation to chest compression ratio of 2:30 than a chest compression to ventilation ratio of 30:2 to reduce the hands-off time & the time to set the CPR. 2) We would like to evaluate possibility of increasing for chest compression accuracy of a ventilation to chest compression ratio of 2:30 than a chest compression to ventilation ratio of 30:2 3) We would like to evaluate possibility of increasing for ventilation accuracy of a ventilation to chest compression ratio of 2:30 than a chest compression to ventilation ratio of 30:2 Methods: According to 2005 American Heart Association Guidelines, 60 paramedic students(20 students X freshmen, sophomore, junior) performed 5 cycles of 3~ chest compressions : 2 ventilations after A, B, C evaluation with Laerdal Resusci R Anne SkillReporters. After 5 minutes rest, the 60 students performed 5 cycles of 2 ventilations : 30 chest compressions after A, B evaluation with the manikins between 13 and 17 September 2010. The short reports including speed & accuracy of chest compression, respiratory, CPR cycle were gained from the manikins. Hands-off times were measured by assistants. Results: Recently, the importance of high quality CPR was emphasized in order to perform the CPR faster and more accurate. To find out improving the conventional CPR method, we switch the procedure of the compression and the ventilation. By switching the procedure back and forth, we are able to compare the effectiveness of CPR between two type of CPR method which are 2:30 and 30:2 methods. 2:30 is that the breaths is delivered twice, first and perform 30 compressions while 30:2 perform 30 compressions first and give 2 breaths followed by the ABC method. Also, we verify the effectiveness of the hands off time, compression accuracy of the compression through the comparison of the two procedure as mentioned earlier. Consequently research verified that 2:30 is the efficient by providing faster set up delivering more accurate chest compression. Conclusion: 2:30 can minimize a time delay from cardiac standstill until starting the chest compression. In addition, hands-off time which is an interruption in chest compression can be shortened by 2:30 method, which result to effective oxygenation of coronary artery & maintenance of the bloodstream. Once again, performing the 2:30 method provide lessen hands off time and increase the accuracy of the chest compression.

• 한국융합학회논문지
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• 제10권2호
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• pp.331-337
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• 2019

연구목적 : 4x, 30x, 30c, and 200c 농도의 동종약물 Arnica montana (A. montana)를 1차 배양된 생쥐 연골세포에 적용하여 염증관련 인자의 변화를 관찰하고자 하였다. 연구방법 : 본 연구는 collagen type II (Coll-2), cyclooxygenase-2 (COX-2) 발현 그리고 prostaglandin 2 (PGE2) 분비에 대해 조사하였다. 결과: 4x, 30x 그리고 30c의 A. montana를 처리하였을 때, Coll-2의 mRNA 발현이 감소하였으며, 30x A. montana의 경우 COX-2 mRNA의 발현이 증가하였다. 또한 COX-2 단백질 발현은 30x와 30c의 A. montana 처리 시 증가함을 보였다. PGE2 분비 또한 30c에서 증가함을 관찰하였다. 결론 : A. montana 처리에 따라 생쥐의 연골세포의 분화 억제를 확인하였으며, 염증관련 인자인 COX-2 및 PGE2의 발현이 증가함을 확인하였다.

• 한국측량학회지
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• 제36권1호
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• pp.17-24
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• 2018

DEM (Digital Elevation Model) is a useful dataset which represents the earth surface. Beside many applications, production and frequent update of DEM is a costly task. Recently global satellite based DEMs are available which has huge potential for application. To check the accuracy, this study compares two global DEMs: AW3D30 (Advanced Land Observing Satellite World 3D 30m) and SRTM30 (Shuttle Radar Topography Mission Global 30m) with reference resampled LiDAR DEM 30m in a test area around Chuncheon, Korea. The comparison analysis was based on statistics of each DEM, their difference, profiles, slope, basin and stream orders. As a result, it is found that SRTM30 and AW3D30 were much similar but inconsistent in the test area compared to the LiDAR30 DEM. In addition, SRTM30 shows less difference with LiDAR30 compared to the AW3D30 DEM. But, DEMs should be very carefully examined for area which has temporal or season changes. For basin and stream analysis, global DEMs can be used only for regional scale analysis not local large scales.

• Molecules and Cells
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• 제39권4호
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• pp.299-309
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• 2016

We have previously reported the role of miR-217 in anti-cancer drug-resistance. miRNA array and miRNA hybridization analysis predicted miR-30a-3p as a target of miR-217. miR-30a-3p and miR-217 formed a negative feedback loop and regulated the expression of each other. Ago1 immunoprecipitation and co-localization analysis revealed a possible interaction between miR-30a-3p and miR-217. miR-30a-3p conferred resistance to anti-cancer drugs and enhanced the invasion, migration, angiogenic, tumorigenic, and metastatic potential of cancer cells in CAGE-dependent manner. CAGE increased the expression of miR-30a-3p by binding to the promoter sequences of miR-30a-3p, suggesting a positive feedback loop between CAGE and miR-30a-3p. miR-30a-3p decreased the expression of p53, which showed the binding to the promoter sequences of miR-30a-3p and CAGE in anti-cancer drug-sensitive cancer cells. Luciferase activity assays showed that p53 serves as a target of miR-30a. Thus, the miR-30a-3p-CAGE-p53 feedback loop serves as a target for overcoming resistance to anti-cancer drugs.

• 한국지진공학회논문집
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• 제11권1호
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• pp.45-57
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• 2007

미국 서부 지역을 근간으로 도출된 30m 심도까지의 평균 전단파속도(Vs30)는 부지 증폭 정도에 따른 설계 지진 지반 운동 결정을 위한 현행 지반 분류 기준이다. 부지의 Vs30을 산정하기 위해서는 현장탄성파 시험으로부터 적어도 30m 심도까지의 전단파 속도(Vs) 분포를 획득해야 한다. 그러나 많은 경우에서 현장의 불리한 여건 및 적용 시험 기법의 제한으로 인해 Vs분포 결정 심도가 30m에 이르지 못할 수 있다. 본 연구에서는 국내 총 72개소 부지들에서 다양한 탄성파 시험 수행을 통해 30m 이상 심도까지 Vs 분포를 획득하여 Vs30과 30m보다 얕은 심도까지의 평균 전단파속도(VsDs)들을 산정하고, 이로부터 Vs30과 VsDs간의 상관관계를 도출하였다. 또한, 모든 Vs 분포 자료의 평균에 근거한 형상 곡선을 작성하여 Vs 분포를 얕은 심도부터 30m까지 외삽할 수 있는 기법을 개발하였다. 얕은 심도 Vs 분포로부터의 Vs30 산정을 위하여 VsDs와 형상 곡선을 이용하는 두 기법은 최하단 Vs를 30m 심도까지 동일하게 연장하는 단순 기법에 비해 편향 정도가 적었으며, 특히 최소 10m 이상 심도까지 확보된 Vs분포의 경우 유용하게 적용될 수 있을 것으로 보인다.

• Animal cells and systems
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• 제8권3호
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• pp.197-203
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• 2004

CD30 is a member of tumor necrosis factor receptor (TNFR) superfamily and has pleiotropic functions including cell activation, proliferation, differentiation, and death, depending on cell types and stage of differentiation. Although CD30 expression has been described mainly in hematopoietic tissues, several types of nonhematopoietic tumors including embryonic carcinoma and germ-cell tumors express CD30. We examined CD30 distribution in the testis and epididymis from wild type and CD30-deficient mice. In the testis, spermatogonia, spermatocytes and Sertoli cells expressed CD30, but not in spermatids. Spermatogonia and spermatocytes near the basement membrane strongly reacted to anti-CD30. In the epididymis, CD30 expression was exclusively observed in luminal epithelia and some interstitial cells. Taken together, these results show a spatio-temporal regulation of CD30 expression in mouse testis and epididymis and suggest a possible role of CD30 in spermatogonia and spermatocytes.

• 한국식품과학회지
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• 제30권2호
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• pp.397-406
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• 1998

난백 lysozyme은 박테리아 세포벽을 선택적으로 분해하므로 식품 가공 공정에 있어서 천연 식품보존제로서의 이용 가치가 높다. 기존의 결정화와 냉동건조 방법 대신 PM30 막을 사용하여 한외여과함으로써 13종의 난백 단백질로부터 single-step에 의해 lysozyme을 분리하고자 하였다. 냉동 건조한 lysozyme을 pH 4.6 citrate-phosphate buffer에 녹여 PM30 막으로 한외여과시 시료 농도, 온도, 막 횡단 압력, 교반속도 등의 운용 조건을 변화시켜주면서 flux를 최대화하는 최적 막분리 조건을 구하였다. 최적 막 분리 조건하에서 시간이 경과함에 따라 막 재질과 막 침착이 flux에 미치는 효과를 측정하였고, 막 분리 여액내 단백질 농도와 lysozyme 농도, 비활성도를 측정하였다. PM30 막을 이용한 난백 lysozyme의 막 분리 최적 조건은 시료농도 0.25%, 온도 $35^{\circ}C$, 막 횡단 압력 30 psi, 교반속도 300 rpm 이었다. 막 분리 초기 12분까지는 YM30 막의 flux가 더 높았으나, 정상상태에서의 flux는 PM30 막이 더 높았다. PM30 막의 분리 여액내 단백질 농도와 lysozyme 농도는 YM30 막에 비해 낮았으나, 비활성도는 2배 이상 높았다. 5회 막 분리한 PM30 막은 새 PM30 막에 비하여 flux가 약 30% 감소하였으나 시간 경과에 따른 flux 감소 경향은 거의 동일하였으며, 막 분리 35분 경과 후 정상상태에 이르러 초기 flux 약 70%를 유지하였다. 막 분리 여액내 lysozyme 농도와 비활성도는 각각 110 units/mL, 2,821 units/mg protein이었으므로, PM30 막을 이용한 한외여과 공정은 천연 항균 효소제인 lysozyme을 분리하는데 매우 효과적인 방법이었다.

• Parasites, Hosts and Diseases
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• 제42권4호
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• pp.195-200
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• 2004

We investigated the induction of resistance to Haemaphysalis longicornis infestation in rabbits that had been immunized with recombinant H. longicornis P27/30 protein. The success of immunological control methods is dependent upon the use of potential key antigens as tick vaccine candidates. Previously, we cloned a gene encoding 27 kDa and 30 kDa proteins (P27/30) of H. longicornis, and identified P27/30 as a troponin I-like protein. In this study, rabbits that were immunized with recombinant P27/30 expressed in Escherichia coli showed the statistically significant longer feeding duration for larval and adult ticks (P<0.05), low engorgement rates in larval ticks (64.4%), and an apparent reduction in egg weights, which suggest that H. longicornis P27/30 protein is a potential candidate antigen for a tick vaccine. These results demonstrated that the recombinant P27/30 protein might be a useful vaccine candidate antigen for biological control of H. longicornis.

• 한국실내디자인학회:학술대회논문집
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• 한국실내디자인학회 2005년도 추계학술발표대회 논문집
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• pp.48-51
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• 2005

The purpose of this study is to provide a basic study for a desirable bathroom planning of the 30s pyung apartments. This study explores the change of the bathroom planning and analyzes the bathroom plans of the existing apartments. The data are 927 case of the bathroom plans of the 30s pyung apartments constructed in Seoul and the new town between 1971-2003. Where 145,807 households live. In this study, a trend of the bathroom form, numble in the 30s pyung apartments are examined. For the better planning of the bathroom. factors like family size. life cycle, and manner of bathroom use should be analyzed in the future studies. The analysis of the dweller's consciousness, satisfaction, and the demands on the bathroom in 30s pyung apartments, and to give useful information on bathroom planning of 30s pyung apartments.

• Asian Pacific Journal of Cancer Prevention
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• 제14권4호
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• pp.2383-2386
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• 2013

Senescence marker protein 30 (SMP30), a hepatocellular carcinoma (HCC) associated antigen, was earlier shown by our research group to be highly expressed in HCC paracancerous tissues, but have low levels in HCC tissues. In order to detect anti-SMP30 antibody in serum of HCC patients, we established pET30a-SMP30 and pColdIII-SMP30 expression systems in Escherichia coli. However, the expression product was mainly in the form of inclusion bodies. In this research, we used several combinations of chaperones, four molecular chaperone plasmids with pET30a-SMP30 and five molecular chaperone plasmids with pColdIII-SMP30 to increase the amount of soluble protein. Results showed that co-expression of HIS-SMP30 with pTf16, combined with the addition of osmosis-regulator, and a two-step expression resulted in the highest enhancement of solubility. A total of 175 cases of HCC serum were studied by ELISA to detect anti-SMP30 antibody with recombinant SMP30 protein. Some 22 were positive and x2 two-sided tests all showed P>0.05, although it remained unclear whether there was a relationship between positive cases and clinical diagnostic data.