• Parasites, Hosts and Diseases
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• v.58 no.3
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• pp.333-338
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• 2020

A small survey was performed to investigate the recent infection status of Clonorchis sinensis and other zoonotic trematode metacercariae in freshwater fish from a local market of Yen Bai city, Yen Bai province, northern Vietnam. A total of 118 fish in 7 species were examined by the artificial digestion method on March 2016. The metacercariae of 4 species of zoonotic trematodes, i.e., C. sinensis, Haplorchis pumilio, Haplorchis taichui, and Centrocestus formosanus, were detected. The metacercariae of C. sinensis were found in 62 (69.7%) out of 89 fish (5 species), and their intensity of infection was very high, 81.2 per fish infected. Prevalences of 3 intestinal flukes, H. pumilio, H. taichui and C. formosanus, were 75.0%, 47.6%, and 31.7% in positive fish species, respectively, with the metacercarial intensities of 15.5, 10.3, and 2.2 per fish infected. From the above results, it has been confirmed that various species of freshwater fish continue to play the role of the infection source of C. sinensis and other zoonotic trematodes in Yen Bai city, Yen Bai province, northern Vietnam. It is of particular note that the prevalence and intensity of C. sinensis metacercariae are much higher than those reported in previous studies in fish in northern Vietnam.

• Korean journal of applied entomology
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• v.35 no.1
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• pp.66-73
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• 1996

For the effective control of mosquito larvae, Anopheles sinensis, the expression vector pCYASK5-1 containing cryIVD gene of Bacillus thuringiensis subsp. morrisoni PG-14 was constructed and transformed into the cyanobacterium Synechocystis PCC6803. The transformants were selected on BG-11 medium containing kanamycin. The expression of cryIVD gene in transformant was confirmed by SDS-PAGE and Western blot analysis. The mortality of A. sinensis larvae was scored for 3 days. Furthermore, growth and distribution rate of transformant were examined. The results showed that Synechocystis PCC6803 transformed with cryIVD gene of B. thuringiensis subsp. morrisoni PG-14 was highly toxic to A. sinensis larvae, demonstrating that it will be a potential agent for mosquito control.

• Parasites, Hosts and Diseases
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• v.53 no.1
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• pp.65-75
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• 2015

Clonorchis sinensis habitating in the bile duct of mammals causes clonorchiasis endemic in East Asian countries. Parkin is a RING-between-RING protein and has E3-ubiquitin ligase activity catalyzing ubiquitination and degradation of substrate proteins. A cDNA clone of C. sinensis was predicted to encode a polypeptide homologous to parkin (CsParkin) including 5 domains (Ubl, RING0, RING1, IBR, and RING2). The cysteine and histidine residues binding to $Zn^{2+}$ were all conserved and participated in formation of tertiary structural RINGs. Conserved residues were also an E2-binding site in RING1 domain and a catalytic cysteine residue in the RING2 domain. Native CsParkin was determined to have an estimated molecular weight of 45.7 kDa from C. sinensis adults by immunoblotting. CsParkin revealed E3-ubiquitin ligase activity and higher expression in metacercariae than in adults. CsParkin was localized in the locomotive and male reproductive organs of C. sinensis adults, and extensively in metacercariae. Parkin has been found to participate in regulating mitochondrial function and energy metabolism in mammalian cells. From these results, it is suggested that CsParkin play roles in energy metabolism of the locomotive organs, and possibly in protein metabolism of the reproductive organs of C. sinensis.

• Mycobiology
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• v.49 no.3
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• pp.267-274
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• 2021

Two Diaporthe species isolated from fruit of Citrus sinensis in China were characterized based on morphology and multilocus phylogeny of ITS, tef1, and tub2 gene sequences. The phylogeny indicated that the two species match Diaporthe taoicola and D. siamensis. A critical examination of phenotypic characteristics confirmed the phylogenetic results. Diaporthe taoicola was morphologically characterized by producing Alpha conidia with tapering toward both ends. Meanwhile, D. siamensis produced cylindrical or ellipsoidal Alpha conidia with two oil drops. Pathogenicity tests revealed that both species were pathogenic to fruit of C. sinensis. To our knowledge, the two species were firstly reported on Citrus sinensis in China.

• The Korean Journal of Mycology
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• v.49 no.3
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• pp.417-423
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• 2021

Apple decline symptoms were frequently observed on cv. Fuji apple orchards located in Gyeonggi, Gyeongbuk, and Gangwon provinces during surveys conducted from May until the end of September 2020. Three fungal strains were isolated from the margins of internal lesions of diseased apple trees, and their morphological characteristics were considered similar to Botryosphaeria sinensis. Phylogenetic analysis using internal transcribed spacer (ITS) regions, translation elongation factor 1-alpha (tef1), beta-tubulin (tub2), and the second largest subunit of RNA polymerase II (rpb2) gene sequences confirmed the closest relationship of isolates with B. sinensis at the species level. According to a pathogenicity test, the appearance of dark-brown discolorations and vascular necrosis on apple branches inoculated with the isolated strain KNUF-20-014 was observed. To the best of our knowledge, this is the first report of B. sinensis as the causal agent of apple disease in Korea.

• Molecular & Cellular Toxicology
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• v.3 no.3
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• pp.185-189
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• 2007

Gene expression levels of choriogenin, vitellogenin and estrogen receptor were determined using Reverse transcription (RT)-PCR technique after exposure to estrogenic chemical bisphenol A in the Korean wild medaka (Oryzias sinensis). These genes have been known to be induced in male test fish when the fish are exposed to estrogenic chemicals. Therefore they can be suggested as a possible biomarker of endocrine disruption in fish, however, relatively little has been known about these genes expression by estrogenic chemicals in Korean wild fish. Mature male Oryzias sinensis were treated with bisphenol A at nominal concentrations of 0.02, 0.2 and 2 mg/L for 6 days and total RNA was extracted from the livers of treated fish for RT-PCR. When the five biomarker genes were amplified by RT-PCR in the same condition, mRNA induction level of each gene was elevated with different sensitivities. Conclusively, the results of this work indicated that measurement of vitellogenin and choriogenin using RT-PCR is effective as a simple tool for the screening of estrogenic chemicals and suggested that O. sinensis would be a suitable model fish for the environmental risk assessment of potential endocrine disruptors.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.11
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• pp.1792-1798
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• 2013

Cedrela sinensis is a Korean traditional wild herb that has a special taste and aroma. This study is conducted to investigate the effect of Cedrela sinensis powder on the antioxidant activity and the quality characteristics of Maejakgwa (Korean traditional cookie). Maejakgwa was prepared with different amounts of Cedrela sinensis powder (in ratios of 0, 5, 10, 15 and 20% to the flour quantity). The antioxidant activity was estimated by DPPH free radical scavenging activity and the total phenol content in Cedrela sinensis powder and Maejakgwa. For analyzing the quality characteristics, the bulk density and pH of the dough, moisture contents, volume, color, texture profile analysis, and sensory evaluations were measured. The bulk density, moisture contents, volume, total phenol contents and DPPH free radical scavenging activity of Maejakgwa significantly increased with increasing Cedrela sinensis powder (P<0.001), while the pH of the dough, L values and b values of Maejakgwa significantly decreased with increasing Cedrela sinensis powder content (P<0.001). The consumer acceptability score for 10% Cedrela sinensis Maejakgwa ranked significantly higher (P<0.001) than those of the other groups in overall preference, flavor, taste, crispiness and color. From these results, we suggest that Cedrela sinensis is a good ingredient for increasing the consumer acceptability and functionality of Maejakgwa.

• Journal of Fisheries and Marine Sciences Education
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• v.29 no.3
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• pp.702-710
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• 2017

Recently, in many reptiles (14 genera of turtles in five families), common characteristics of incubation temperatures are known to determine the sexes of hatchlings in many species of turtles, including the map turtles, painted turtles and snapping turtles, emys turtle, etc. According to many researcher's reports, in general, incubation at $25^{\circ}C$ (cooler temperatures) produces all or mostly males, however, incubation at $31^{\circ}C$ (higher temperatures) or higher produces all or mostly females. Exceptionally, even cooler temperature ($20^{\circ}C$) produce females, they produced all or mostly females. Accordingly, it is well-known that incubation temperature is the sex determining agent in these turtles. However, this paper presents study of the sex ratio and nest ecology in natural spawning nest: Observations on hatching sex ratios of eggs collected from natural nests of T. sinensis are similar to a previous report of the same genus Trionyx in the soft-shelled turtles. However, this genus (or species) showed some different phenomena to other kinds of turtles such as various kinds turtles mentioned above. After collection of naturally spawned eggs (17 eggs of T. sisnensis) on the natural nests, a laboratory experiment by the constant incubation temperatures was conducted with natural fluctuating soil temperatures in the natural nest with the soft-shelled turtle, T. sinensis. And also laboratory experiments were conducted using constant incubation temperatures of $25^{\circ}C$ (cooler temp.) and $30^{\circ}C$ (higher temp.) with the turtle, T. sinensis. Exceptionally, it was confirmed that the first and second incubation temperatures can't control sex-determination in the freshwater soft-shelled turtle, T. sinensis. The sex ratio approximated 1:1 (${\chi}^2=0.06$, P>0.05 (the Ist experiment). And the sex ratio approximated 1:1 independently of incubation temperature (${\chi}^2=0.33$, P>0.05 (the 2nd experiment). Consequently, temperature has no effect on sex determination in the genus Trionyx in a soft-shelled turtle.

• The Korean Journal of Food And Nutrition
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• v.31 no.4
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• pp.464-470
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• 2018

All the parts of the Cedrela sinensis A. Juss., including the seeds, roots, and leaves, have been known to exert medicinal effects. The C. sinensis and its major compound, quercetin, were previously reported to exhibit the anti-inflammatory and anti-oxidative activities. However, the hepatoprotective effects of the C. sinensis leaves against the alcohol-induced oxidative stress in the HepG2 cells have not been studied. In this study, we investigated the antioxidant activities and analyzed the flavonoid contents of the C. sinensis-leaf extract (CE). The total flavonoid contents of the CE is 1,874.5 mg/100 g dry weight (DW), while the total quercetin 3-O-rhamnoside (quercitrin) contents, which was identified as the major flavonol in the CE, is 1,456.0 mg/100 g DW. In the ethanol-stimulated HepG2 cells, the CE effectively prevented the cytotoxic effect and increased the gene expression of the antioxidant enzymes, such as the heme oxygenase-1 (HO-1) and the glutathion peroxide (GPx). The level of the reactive oxygen species (ROS) production was significantly decreased in the CE-treated HepG2 cells. In conclusion, the C. sinensis extract suppressed the alcohol-induced oxidative stress in the HepG2 cells via the induced GPx and HO-1 gene expressions. It is expected the CE positive effects will likely be attributed to the flavonoids, like the quercetin, within the CE.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.20 no.2
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• pp.79-91
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• 2017

This study was conducted to find out the physical properties and soil shearing strength reinforcement effect of herbaceous plants for the slope revegetation works. Two native plants(Pennisetum alopecuroides and Miscanthus sinensis) were used for this experiment, because they have excellent seed germination rates without preconditioning, and grow naturally around rivers. To identify the physical properties, the partial dry weight of plants were investigated. To identify the soil shearing strength reinforcement effect, the respective soil shearing strengths of the control soils, Pennisetum alopecuroides, Miscanthus sinensis samples were measured. Also, we did a correlation analysis to examine the relation of shearing strength to plant features. The results are summarized as follows: 1. The average dry weight of Pennisetum alopecuroides samples consists of 52.36% above ground and 47.64% at root. And in dry weight, 78.24% of it's root distributes within 10 cm in soil depth. Meanwhile the average dry weight of Miscanthus sinensis samples consists of 52.91% above ground and 47.09% at root. And in dry weight, 82.95% of it's root distributes within 10 cm in soil depth. 2. The results of correlation analysis showed that for both Pennisetum alopecuroides and Miscanthus sinensis, it could not be said that there was any correlation between shearing strength and plant characteristics, and statistically they were not meaningful. 3. In the shearing strength test with control soils, Pennisetum alopecuroides, Miscanthus sinensis as subjects, the differences in shearing strength measurement results were modest, and the order was shown as control soils < Pennisetum alopecuroides < Miscanthus sinensis, so the soil shearing strength reinforcement effect by the Pennisetum alopecuroides and the Miscanthus sinensis on loamy sand at river banks surface was confirmed.