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Pathogenic and Molecular Characteristics of Agrobacterium vitis strains isolated from Grapevine in Korea

  • Kim, J.G.;Kim, S.H.;Choi, J.E.;Lee, Y.K.;Kang, H.W.
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.120.2-120
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    • 2003
  • Agrobacterium vitis is a causal agent of crown-gall disease on grapevine. In Korea, grapevine variety (GeoBong) have severely been infected by the bacteria since stems of the variety were buried in soil for overwintering. Infection ratio over 70-80% was observed on 7 years old GeoBong grapevine in Ansung and Cheonan. PCR specific primers for A. vitis strains were designed using nucleotide sequences of vir A gene in Ti-Plasmid, pheA gene in chromosomal DNA and a URP-PCR polymorphic band. Three hundred bacterial strains were isolated from the different 80 galls formed on GeoBong grapevine in Cheonan and Ansung of Korea and were screened to identify A. vitis using the three specific PCR primers for Agrobacterium vitis. Twenty-four bacterial strains that are detected by the primers were further confirmed by pathogenicity and biochemical methods. To investigate the genomic diversity of the bacterial strains, twenty primers of 20 mer referred to universal rice primers (URP) were applied for PCR fingerprinting, Of them, URP2R and URP2F primers could effectively be used to detect polymorphism within the bacterial strains.

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Regeneration Process of Subalpine Coniferous Forest in Mt. Jiri (智異山 亞高山帶 針葉樹林의 更新)

  • Kang, Sang Joon
    • The Korean Journal of Ecology
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    • v.7 no.4
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    • pp.185-193
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    • 1984
  • Regneration process of Abies koreana-Pinus koraiensis community consisted of a subalpione coniferous forest in Mt. Jiri, Korea, was studied in relation to age structure, especially to gap formation. The tall-tree layer (ca. 6.5m) is dominated by Abies koreana and Pinus koraiensis, while lower layer by the sapling and juveniles of A. koreana and Picea jezoensis below 2m tall. The ranges of DBH in A. koreana, P. koraiensis and P. jezonesis were 11.8cm~26.4m, 11.7cm~24.5 cm and 18.2cm~21.7 cm, respectively. The trees below 130 cm tall had contagious distribution, while tall and subtall trees had uniform distribution. The average tree ages of A. koreana, P. koraieniensis and P. jezoensis were 60~70 years, 70~80 years and 70~90 years, respectively. The saplings and juveniles below 20 years in tree ages were occupied over 80% of total trees. The coniferous trees in the gaps or around dead trees were composed of sapligs and juveniles which had emerged or invaded about 20 years before and after the gap formation. The Betula type regeneration of the coniferous forest took place in gaps which orginated from the failing down of a single or a few trees by longevity. Accordingly, it is clear that the subalpine coniferous forest composed of A. koreana of A. koreana, P. koraiensis and P. jezoensis in Mt. jiri was supporting by the regeneration pattern of Betula type.

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Fabrication of the Cu-STS-Cu Clad Metal for High Strength Electric Device Lead Frame and Thermal Stability on Their Physical Properties (고강도 전자소자 리드프레임용 Cu/STS/Cu 클래드 메탈제조 및 물리적특성에 대한 열안정성 연구)

  • Kim, Il-Gwon;Son, Moon-Eui;Kim, Young-Sung
    • Journal of Welding and Joining
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    • v.32 no.5
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    • pp.80-86
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    • 2014
  • We have successfully fabricated high strengthening Cu/STS/Cu 3 layered clad metal of $70kgf/mm^2$ grade for electric device lead frame, and investigated thermal effect of the mechanical and physical properties on the Cu/STS/Cu 3 layered clad metal lead frame material at different temperatures ranging from RT to $200^{\circ}C$. The fabricated clad metal shows a good thermal stability under 6% degrading of mechanical tensile strength and hardness change at $200^{\circ}C$ and also physical properties show stable thermal and electrical conductance of over $220W/m{\cdot}K$ and 58.44% IACS upto the $200^{\circ}$. The results confirm that fabricated high strengthening Cu/STS/Cu 3 layered clad metal can be applied for the high performed electrical lead frame devices.

Effects of Volatile Solid Concentration and Mixing Ratio on Hydrogen Production by Co-Digesting Molasses Wastewater and Sewage Sludge

  • Lee, Jung-Yeol;Wee, Daehyun;Cho, Kyung-Suk
    • Journal of Microbiology and Biotechnology
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    • v.24 no.11
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    • pp.1542-1550
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    • 2014
  • Co-digesting molasses wastewater and sewage sludge was evaluated for hydrogen production by response surface methodology (RSM). Batch experiments in accordance with various dilution ratios (40- to 5-fold) and waste mixing composition ratios (100:0, 80:20, 60:40, 40:60, 20:80, and 0:100, on a volume basis) were conducted. Volatile solid (VS) concentration strongly affected the hydrogen production rate and yield compared with the waste mixing ratio. The specific hydrogen production rate was predicted to be optimal when the VS concentration ranged from 10 to 12 g/l at all the mixing ratios of molasses wastewater and sewage sludge. A hydrogen yield of over 50 ml $H_2/gVS_{removed}$ was obtained from mixed waste of 10% sewage sludge and 10 g/l VS (about 10-fold dilution ratio). The optimal chemical oxygen demand/total nitrogen ratio for co-digesting molasses wastewater and sewage sludge was between 250 and 300 with a hydrogen yield above 20 ml $H_2/gVS_{removed}$.

Effect of Solution Temperature on the Cavitation Corrosion Properties of Carbon Steel and its Electrochemical Effect

  • Jeon, J.M.;Yoo, Y.R.;Kim, Y.S.
    • Corrosion Science and Technology
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    • v.20 no.6
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    • pp.325-334
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    • 2021
  • In the open system (vessel and pipe), the maximum corrosion rate of carbon steel at ca. 80 ℃ was obtained due to the decrease of dissolved oxygen by increasing the solution temperature. Effect of temperature on the cavitation damage can be explained through several mechanisms. Moreover, when cavitation occurs on the surface of metal and alloys, whether cavitation is erosion or corrosion is still controversial. This work focused on the effect of solution temperature on the corrosion of carbon steel under cavitation in an open system, Tests were performed using an electrochemical cavitation corrosion tester in 3.5% NaCl solution and the effect of solution temperature of carbon steel was discussed. Cavitation corrosion rate can be increased by cavitation, but when the temperature increases, a dissolved oxygen content reduces at a very high speed and thus the maximum cavitation corrosion temperature changed from 80 ℃ to 45 ℃. Below the maximum cavitation temperature, the electrochemical effect was more dominant than the mechanical effect by increasing temperature, but over the maximum cavitation temperature, the mechanical effect was more dominant than the electrochemical effect by increasing temperature.

The Physical Properties and Dyeability of the Degummed and Sericin Fixed Silk Fabrics (정련 및 세리신 정착처리 견직물의 물리적 성질과 염색성)

  • 이은미;이혜자;유혜자
    • Journal of the Korean Society of Clothing and Textiles
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    • v.27 no.5
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    • pp.517-523
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    • 2003
  • We studied the physical properties of silk fabrics after degumming, the dyeability and the color fastness of silk fabrics after degumming and sericin fixing. As the sericin was removed from silk fabrics, the rate of weight loss increased and both the abrasion resistance and the drape coefficient decreased. This means that the amount of the sericin remained in silk fabrics significantly affects the physical properties of silk fabrics. On the surface and the cross-section of silk fabrics, the silk fibers enclosed by the sericin seemed to be in a lump shape. Each fibroin strand, however, got scattered, as the process of degumming went through. The dyeability of silk fabrics degummed decreased at between 20$^{\circ}C$∼80$^{\circ}C$ the dyeing temperature, on the other hand, it significantly increased over 80$^{\circ}C$. The dyeability of the sericin-fixed silk fabrics was lower than that of the non-serin-fixed silk fabrics, to a little extent. The colorfastness of crocking in the dyed-silk fabrics was a little low and that of the sweat was much lower in a basic sweat. Especially, the colorfastness of the partially degummed silk fabrics was low, because the sericin was not stable in the condition of sweat. Therefore, the process of sericin fixing is essentially required, for the partially degummed silk fabrics and the process of degumming itself.

Effects of microstructure and welding heat input on the toughness of weldable high strength steel weldments (용접구조용 고장력강의 용접부 인성에 미치는 미세 조직과 용접 입열량의 영향)

  • 장웅성;방국수;엄기원
    • Journal of Welding and Joining
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    • v.7 no.3
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    • pp.44-54
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    • 1989
  • This study was undertaken to evaluate the allowable welding heat input range for high strength steels manufactured by various processes and to compare the weldability of TMCP steel for high heat input welding with that of conventional Ti-added normalized steel. The allowable welding heat input ranges for conventional 50kg/$mm^2$ steel to guarantee D or E grade of ship structural steel were below 150 and 80kJ/cm respectively. Such a limit in welding heat input was closely related with the formation of undesirable microstructures, such as grain boundary ferrite and ferrite side plate in the coarse grain HAZ. In case of 60 and 80kg/$mm^2$ quenched and tempered steels, for securing toughness in weldments over toughness requirements for base metal, each welding heat input had to be restricted below 60 and 40kJ/cm, that was mainly due to coarsened polygonal ferrite in weld metal and lower temperature transformation products in coarse grain HAZ. The TMCP steel could be appropriate as a grade E ship hull steel up to 200kJ/cm, but the Ti-added normalized steel could be applied only below 130kJ/cm under the same rule. This difference was partly owing to whether uniform and fine intragranular ferrite microstructure was well developed in HAZ or not.

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Preparation and Quality of Uncooked-Colored Wine Using Black Rice (흑미를 이용한 무증자 유색주의 제조와 품질)

  • 김순동;김미향;함승시
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.29 no.2
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    • pp.224-230
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    • 2000
  • In order to study the quality of uncooked-colored wine by using polished black rice(PBR) and glucoamylase, the mixture ration of PBR ws investigated. The growths of yeast and lactic acid bacteria, pH and alcohol concentration of the colored wine prepared by adding PBR in the range of 20 to 100% were higher than those of polished rice only, whereas the contents of residual sugars, total free amino acids and fusel oil of the colored wine were lower. The colored wine prepared by 20% PBR and that of over 80% PBR showed a light red color and a dark red, respectively. The optimal addition ratios of PBR evaluated by palatability of color and flavor, and sensory overall quality was 40 to 60%.

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A Research of User Experience on Multi-Modal Interactive Digital Art

  • Qianqian Jiang;Jeanhun Chung
    • International Journal of Internet, Broadcasting and Communication
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    • v.16 no.1
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    • pp.80-85
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    • 2024
  • The concept of single-modal digital art originated in the 20th century and has evolved through three key stages. Over time, digital art has transformed into multi-modal interaction, representing a new era in art forms. Based on multi-modal theory, this paper aims to explore the characteristics of interactive digital art in innovative art forms and its impact on user experience. Through an analysis of practical application of multi-modal interactive digital art, this study summarises the impact of creative models of digital art on the physical and mental aspects of user experience. In creating audio-visual-based art, multi-modal digital art should seamlessly incorporate sensory elements and leverage computer image processing technology. Focusing on user perception, emotional expression, and cultural communication, it strives to establish an immersive environment with user experience at its core. Future research, particularly with emerging technologies like Artificial Intelligence(AR) and Virtual Reality(VR), should not merely prioritize technology but aim for meaningful interaction. Through multi-modal interaction, digital art is poised to continually innovate, offering new possibilities and expanding the realm of interactive digital art.

A Patient's Satisfaction with Denture in the Old People (노인들의 의치 장착 후의 만족도에 관한 연구)

  • Lee, In-Kyu;Song, Yun-Hee
    • Journal of Technologic Dentistry
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    • v.23 no.2
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    • pp.61-74
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    • 2002
  • This experiment was to find out the complacency of wearing dentures and the linkage to the quality of life style of oldsters 60 years old and over. 122 numbers of oldsters who came to aid to the health center were put to survey. This was taken place within the areas of Deajun, Koonsan, Mooju and Jinahn, thus procured the following result. 1. The length of edentulous period of 1-6years of oldsters aged around 60s showed 26.0%. The oldsters with edentulous period of over 7years aged in the 70s showed 26.1 % and 56.0% on oldsters aged in the 80s. This shows that as the age increases the edentulous period lengthens. (P<0.05) The length of time of using the denture shows. llyears or over on women 41.9%, less than 6years on men 71.4% as the highest rate. 11 years or over on towns/subcounty show 57.5%, small and medium cities more than 1 year 63.6%, less than six years also 63.6% and Kwangyuk city 47.6%. 2. The complacency on medical treatment of dentures was highest in Kwangyuk city of 61.3%, compared to towns/subcounty of 50.8% and small and medium cities of 33.3%. (P<0.05) 3. The complacency on mastication and pronunciation appears, 2.74% in Kwangyuk city, 3.10% in towns/ subcounty which is higher than the small and medium cities showing 1.09% on average. Satisfaction rate tends to be higher as the length of time of using the denture is longer. 4. Inconvenience on eating habits caused by dentures were felt by women. Wanting to get a new denture was 25.6% by women showing much higher rate than that of men which is 2.8% by men. (P<0.05) 5. The complacency of change in their life style after wearing the dentures were higher in Kwangyuk city of 64.5% whereas it showed 27.0% in towns! subcounty and 16.7%in small and medium cities. (P<0.05)

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