• Bulletin of the Korean Chemical Society
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• v.28 no.2
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• pp.175-176
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• 2007

• Bulletin of the Korean Chemical Society
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• v.25 no.7
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• pp.1099-1102
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• 2004

• Proceedings of the Botanical Society of Korea Conference
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• 2001.04a
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• pp.27-27
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• 2001

• Bulletin of the Korean Chemical Society
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• v.23 no.10
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• pp.1473-1476
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• 2002

• Applied Biological Chemistry
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• v.38 no.2
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• pp.179-183
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• 1995

The brassinosteroid-like active principles from immature seeds of Zea mays were purified and identified as castasterone, teasterone, 28-norcastasterone and 6-deoxocastasterone by GC-MS analysis. Our work is probably the first report that showed the presence of brassinosteroids in Zea mays seeds.

• Proceedings of the Zoological Society Korea Conference
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• 2000.10a
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• pp.215.2-216
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• 2000

No Abstract, See Full Text

• Bulletin of the Korean Chemical Society
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• v.24 no.9
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• pp.1385-1388
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• 2003

• Applied Biological Chemistry
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• v.36 no.5
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• pp.376-380
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• 1993

To investigate the presence of the brassinosteroid substances in immature Oryza sativa L. cv Tongjinbyeo seeds, the methanol extract was purified by the sequential use of solvent fractionation, silica gel adsorption chromatography, Sephadex LH-20 chromatography and charcoal adsorption chromatography. The activity of brassinosteroid was monitored by the rice inclination test and its presence could be confirmed in each purification step. The purified active components were separated by silica gel adsorption chromatography and Sephadex LH-20 chromatography. Brassinosteroid substances in separated active fractions were identified as castasterone, teasterone and 6-deoxocastasterone by HPLC. Our work is probably the first report of endogenous brassinosteroids in Oryza sativa seeds. The content of brassinosteroid in Oryza sativa seeds as converted into brassinolide was $0.5{\sim}1.5\;ng/g$ fresh weight.

• Journal of Plant Biotechnology
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• v.30 no.4
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• pp.411-419
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• 2003

The potent biosynthetic precursors, 24$\alpha$-methylcholesterol and 24$\alpha$-methylcholestanol, and the endogenous brassinosteroids (BRs), castasterone (CS) and 6-deoxocastasterone (6-deoxoCS), were identified from shoots of maize seedlings. In addition, the presence for activities of several enzymes involved in the late C6-oxida-lion pathway from 24$\alpha$-methylcholestanol to CS was demonstrated in the plants. However, activity for brassinolide (BL) synthase which catalyze the conversion of CS to BL, the last step of the late C6-oxidation pathway, was not detected in the enzyme solution obtained from the maize shoots. Together with the fact that BL was not identified from the maize shoots, these results strongly suggested that BRs in the maize shoots are biosynthesized during seedling growth and the active BR in the shoots is not BL but CS.