• BMB Reports
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• v.43 no.4
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• pp.263-267
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• 2010

To characterize the biochemical properties of the PP2A regulatory B subunit, PPP2R5D, we analyzed its phosphorylation sites, stoichiometry and effect on holoenzyme activity. PPP2R5D was phosphorylated on Ser-53, Ser-68, Ser-81, and Ser-566 by protein kinase A, and mutations at all four of these sites abolished any significant phosphorylation in vitro. In HEK293 cells, however, the Ser-566 was the major phosphorylation site after PKA activation by forskolin, with marginal phosphorylation on Ser-81. Inhibitory tyrosine phosphorylation on Tyr-307 of the PP2A catalytic C subunit was decreased after forskolin treatment. Kinetic analysis showed that overall PP2A activity was increased with phosphorylation by PPP2R5D phosphorylation. The apparent Km was reduced from $11.25\;{\mu}M$ to $1.175\;{\mu}M$ with PPP2R5D phosphorylation, resulting in an increase in catalytic activity. These data suggest that PKA-mediated activation of PP2A is enabled by PPP2R5D phosphorylation, which modulates the affinity of the PP2A holoenzyme to its physiological substrates.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.14 no.1
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• pp.32-36
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• 1981

Fry of a Siamic catfish Clarias batrachus was brought to Korea on August 14,1979 and exper-imentally reared for a whole life cycle in a small water recycling aquarium. After 29 days since start of feeding the fry were measured 5 cm in 1ength and 1.43 g in body weight and after 260 days they were measured 205.7 g average. Chorionic gonadotrophin (2 IU) was injected to a healthy female (224 g) and after 63 hours fertilized eggs were obtained in a small water recycling aquarium. About 1,300 fry were hatched out 20 hours after spawning at $30^{\circ}C$ constant water temperature. The fry began to feed on water fleas 53 hours after hatching. My sincere thanks are due to Mr. Panu Tavarutmaneegu1, Mr. Chanchai Sansrimahachai and Miss Revadee Spriprasert, National Inland Fisheries Institute, Bangkok, Thailand for their cooperative arrangement to bring the fry of Clarias batrachus to Korea for this experiment.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2002.04a
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• pp.49-53
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• 2002

워싱턴주 벤쿠버시 Bonneville 전력소 전주 보관 지역내 PCP와 creosote 오염토양 2g 당 0.020g heme에 0.108g $H_2O$$_2$을 혼합한 산화방법과 0.035g hemoglobin에 0.324g $H_2O$$_2$을 혼합한 산화방법을 비교ㆍ조사하였다. 오염토양에 $^{14}$ C-PCP을 첨가한 다음에 $^{14}$ C의 물질수지를 조사한 결과, 24시간동안 반응 후 $^{14}$ $CO_2$는 heme 과 hemoglobin반응에서는 각각 3.50g와 3.88% 생산되었다. $^{14}$ C 물질수지 분포는 heme 촉매 산화반응에서 용매 상에 43.01% 토양 상에는 46.03%이고, hemoglobin 촉매 산화반응에서는 용매 상에 39.21%와 토양 상에 51.25%로 비슷한 분포를 보였다. 실험실 규모 pan 실험에서 초기 PCP농도 273$\pm$20 mg/kg과 TPH 6379$\pm$45 mg/kg인 오염토양에서 hemoglobin 촉매 산화 반응이 초기반응을 제외하고 7일 이후 반응에서 heme 촉매 산화반응보다 빠르게 분해되었고, 35일 반응 이후 PCP는 10 mg/kg 이하의 값을 나타내었고, TPH도 유사한 결과를 보여 주었다. 그러므로 건조 hemoglobin과 과산화수소에 의한 PCP 오염토양 복원기술은 분해율이 높고 경제성을 가지고 있으므로 기존의 복원공정을 대안으로 제시될 수 있다.

• Korean journal of applied entomology
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• v.42 no.1
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• pp.1-7
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• 2003

Morphological characteristics, developmental period, and seasonal occurrence of Adeia leucomelas (L.) were investigated from 1999 to 2000. In addition, consumption of sweetpotato as food was also examined. Adults of A. leucomelas were dark-brown and body lengths of females and males were 20.2 mm and 18.9 mm, respectively, Wing expanse of female and male was 33.7 mm and 29.4mm, respectively. Egg was flat round-shape. Larva was light yellow-green to dark-brown with 3.3-53.5 mm. Pupa was deep-brown and 15.1 mm in length. Developmental periods of A. leucomelas from egg to adult emergence at different temperatures of 15, 20, 25, and 30$^{\circ}$C were 108.5, 70.7, 40.2, and 29.1 days, respectively, Developmental threshold (DT) and effective accumulative temperatures were estimated as 10.7$^{\circ}$C and 67.5 DD in egg stage, 11.0$^{\circ}$C and 275.1 DD in larval stage and 9.3$^{\circ}$C and 244.6 DD in pupal stage, respectively. The longevity of adult female was shortened with increment of temperature, whereas the total numbers of eggs laid by a female were increased. The larvae of A.leucomelas occurred from mid-June to early October, and population reached its peak during early to mid-September in Jeonbuk province. Food consumption of A. leucomelas was highest at 20-25$^{\circ}$C. Food consumption of 1 st, 2nd, 3rd, 4th, and 5th larvae of A. leucomelas per day at 25$^{\circ}$C was 0.4, 3.6, 19.6, 40.7, and 78.9 $\textrm{cm}^2$, respectively.

• Restorative Dentistry and Endodontics
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• v.10 no.1
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• pp.43-53
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• 1984

The purpose of this study was to suggest the use of laser energy in the the field of operative dentistry without considerable pulpal damage and significant effects on the dental hard tissue, additionally to find out the methods which could control the temperature rise. The laser beam (CW $CO_2$ laser, output: 6W, beam diameter: 1.5mm) was focused on the center of the occlusal surface of extracted lower molars. A Ge lens (focal length 200mm) was used to focus the primary laser beam. In order to vary the total amount of the same irradiated energy, experimental subjects were devided into three groups: continuously irradiated group, intermittently irradiated group, and water-cooled group after continuous laser irradiation. Temperature changes in the pulp chamber after laser irradiation were measured and recorded by the digital thermometer and recorder. The following results were obtained: 1. Temperatures in the pulp chamber were raised up in the order of the continuously irradiated group, intermittently irradiated group, water-cooled group after continuous laser irradiation. 2. In the continuously irradiated group, the temperature was raised up $1.7^{\circ}C$, $3.8^{\circ}C$, $7.3^{\circ}C$, $17.2^{\circ}C$ after 2, 4, 8, 16 seconds of the irradiation of laser. In the intermittently irradiated group, the changes were $1.2^{\circ}C$, $3.4^{\circ}C$, $6.3^{\circ}C$, $11.1^{\circ}C$, respectively. In the water-cooled group after continuous laser irradiation, the changes were $0.0^{\circ}C$, $0.8^{\circ}C$, $1.6^{\circ}C$, $6.9^{\circ}C$, respectively. 3. The starting time of temperature rise in the pulp chamber had no connection with laser irradiation time.

• Annals of Rehabilitation Medicine
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• v.42 no.6
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• pp.814-821
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• 2018

Objective To suggest rotation angles of fluoroscopy that can bypass the carotid sheath according to vertebral levels for cervical transforaminal epidural steroid injection (TFESI). Methods Patients who underwent cervical spine magnetic resonance imaging (MRI) from January 2009 to October 2017 were analyzed. In axial sections of cervical spine MRI, three angles to the vertical line (${\alpha}$, angle not to insult carotid sheath; ${\beta}$, angle for the conventional TFESI; ${\gamma}$, angle not to penetrate carotid artery) were measured. Results Alpha (${\alpha}$) angles tended to increase for upper cervical levels ($53.3^{\circ}$ in C6-7, $65.2^{\circ}$ in C5-6, $75.3^{\circ}$ in C4-5, $82.3^{\circ}$ in C3-4). Beta (${\beta}$) angles for conventional TFESI showed a constant value of $45^{\circ}$ to $47^{\circ}$ ($47.5^{\circ}$ in C6-7, $47.4^{\circ}$ in C5-6, $45.7^{\circ}$ in C4-5, $45.0^{\circ}$ in C3-4). Gamma (${\gamma}$) angles increased at higher cervical levels as did ${\alpha}$ angles ($25.2^{\circ}$ in C6-7, $33.6^{\circ}$ in C5-6, $43.0^{\circ}$ in C4-5, $56.2^{\circ}$ in C3-4). Conclusion The risk of causing injury by penetrating major vessels in the carotid sheath tends to increase at upper cervical levels. Therefore, prior to cervical TFESI, measuring the angle is necessary to avoid carotid vessels in the axial section of CT or MRI, thus contributing to a safer procedure.

• Journal of Life Science
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• v.18 no.2
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• pp.264-272
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• 2008

The lactate dehydrogenase (EC 1.1.1.27, LDH) isozymes in tissues from Acanthogobius hasta were characterized by biochemical, immunochemical and kinetic methods. The activities of LDH in skeletal muscle and eye tissues were 65.30 and 53.25 units, but LDH activities in heart and liver tissues were very low. LDH/CS (EC 4.1.3.7, citrate synthase) in skeletal muscle was the highest as 22.29. Specific activities of LDH in brain, eye and skeletal muscle were 56.45, 38.04 and 11.0 units/mg, respectively. The LDH isozymes in tissues were separated by polyacrylamide gel electrophoresis after immunoprecipitation with antiserum against $A_4,\;B_4$ eye-specific $C_4$ and liver-specific $C_4$. LDH $AC_4$ isozymes were detected predominantly in skeletal muscle, brain and eye tissues, and $B_4$ isozyme was detected in heart. Anodal eye-specific $C_4$ and cathodal liver-specific $C_4$ were coexpressed in A. hasta. The eye-specific $C_4$ isozyme showed higher activity in eye tissue, but liver-specific $C_4$ isozyme showed lower activity in liver. As a result, one part of molecular structures in $A_4\;and\;C_4,\;A_4\;and\;B_4$, and eye-specific $C_4$ and liver-specific $C_4$ were similar, but in $B_4\;and\;C_4$ were different with each other. Therefore the subunit A may be conservative in evolution, and the evolution of subunit B seems to be faster than that of subunit A. The LDH $A_4$ isozyme of skeletal muscle was purified in the fraction from elution with NAD+ containing buffer of affinity chromatography and eye-specific $C_4$ isozyme was eluted right after $A_4$, so the structure of eye-specific $C_4$ isozyme is similar to $A_4$. And LDH activity remained 35.22-43.47% as a result of the inhibition by pyruvate, the Michaelis-Menten constant values for pyruvate was 0.080-0.098 mM, and Vmax were 153.85 units, 35.09 units in skeletal muscle and eye, respectively. Also the $B_4$ isozyme was the thermo-stablest and $C_4$ was stabler than $A_4$ isozyme. The optimum pH of LDH was 6.5. The results mentioned above indicate that isozymes in tissues showed the properties between LDH $A_4\;and\;B_4$ isozyme as A. hasta was adapted to hypoxic conditions. Also LDH seems to function more effectively under anaerobic condition because LDH in skeletal muscle and eye tissues have high affinity for pyruvate.

• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.1
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• pp.104-110
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• 2015

In order to develop new Kalopanax pictus shoot Jangajji (KJ) with low salt concentration, physicochemical changes of KJ soaked at different concentration of soybean sauce (10~50%) were investigated for 50 days. The pH of KJ increased significantly with increasing concentration of soybean sauce in soaking solution (P<0.05). Titratable acidity and NaCl concentration of all KJ increased during storage for 50 days at $10^{\circ}C$. The total bacterial count slowly increased during early storage time and reached its highest level (4.74 log CFU/g) after storage for 30 days. KJ-20 (KJ added with 20% soy sauce) showed the highest scores for taste, flavor, texture, and overall acceptability. The total polyphenol and flavonoid contents of KJ extract increased with increasing concentration of soybean sauce. DPPH and ABTS radical scavenging activities of KJ extract were 27.22% and 38.37% (control), 40.74% and 43.65% (KJ-10), 41.79% and 50.37% (KJ-20), 43.09% and 52.60% (KJ-30), 48.62% and 53.45% (KJ-40), and 50.85% and 60.79% (KJ-50), respectively.

• Korean Journal of Plant Resources
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• v.22 no.2
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• pp.145-151
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• 2009

This study was performed to investigate the antioxidant activities and the whitening effect of mulberry (Morus alba L.) root bark extracts. The antioxidant activities of water and 70% ethanol extracts of mulberry root bark were 65.8% and 87.0% in the DPPH assay at $1,000\;{\mu}g/ml$ ; 89.3% and 77.1% in the ABTS assay at $1,000\;{\mu}g/ml$. Xanthine oxidase inhibition of water and 70% ethanol extracts were 100% and 96.2% at $1,000\;{\mu}g/ml$. Nitric oxide radical inhibition of water and 70% ethanol extracts showed 43.5% and 53.0% at $500\;{\mu}g/ml$, and it was similar to the BHA effect(43.8%). Tyrosinase inhibitory activities of water and 70% ethanol extracts were 79.6% and 93.5% at $1,000\;{\mu}g/ml$. These results confirm that mulberry root bark has the great potential to be a cosmeceutical ingredient with a natural antioxidant and a skin-whitening effect.

• Journal of Korean Society of Forest Science
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• v.113 no.1
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• pp.97-106
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• 2024

Evaluating carbon (C) and nitrogen (N) inputs from litterfall is important for soil nutrient management to enhance forest productivity and to understand the mechanisms of nutrient cycling in forest ecosystems. This study was conducted to compare C and N inputs from litterfall components of Cryptomeria japonica D. Don an d Chamaecyparis obtusa Endlicher planted in adjacent sites in the Jinju Research and Experimental Forests in Gyeongsangnam-do, South Korea. Litterfall into litter traps was collected at three-month intervals between December 2020 and December 2021, and the C and N concentrations of the litterfall components were measured. Litterfall amounts were not significantly different between the plantations, except for reproductive litterfall components. Litterfall accumulation peaked between December and March. The litterfall C concentration in the needle and seed litterfall was significantly higher for C. obtusa than for C. japonica. By contrast, the C concentrations in needle and flower litterfall differed seasonally. The mean N concentration of needle litterfall was significantly higher in C. japonica (0.96%) and C. obtusa collected between June and September (1.01%) than in the other seasons (C. japonica: 0.43%; C. obtusa: 0.53%). Carbon and N inputs in both plantations were highest in needle litterfall collected from December to March and lowest in needle litterfall collected from June to September. Annual C input by litterfall was similar between the plantations (C. japonica: 3,054 kg C ha^-1 yr^-1; C. obtusa: 3,129 kg C ha^-1 yr^-1), whereas total N input was higher for C. japonica (46.93 kg N ha^-1 yr^-1) than for C. obtusa (25.17 kg N ha^-1 yr^-1). The higher N input in the C. japonica plantation than in the C. obtusa plantation was associated with the input of reproductive components. These results could be applied to improve stand-scale models of C and N cycling by litterfall components in C. japonica an d C. obtusa plantations.