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남조류의 이형세포 조절 유전자와 질소량에 따른 유전자 발현의 분석 (Analysis of a Heterocyst-controlling Gene and Its Expression upon Nitrogen Starvation in a Cyanobacterium)

  • 배정진;윤호성
    • 생태와환경
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    • 제38권4호통권114호
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    • pp.510-517
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    • 2005
  • 선형의 남조류 아나베나는 광합성과 질소고정 능이 있으며, 이는 아마도 이 남조류가 각종 육수 환경에 잘 적응하는데 큰 역할을 했다고 볼 수 있다. 작은 patS라는 유전자는 질소 고정세포의 형성을 막으며 결과적으로 질소원이 부족한 환경에서 아나베나의 죽음을 가져온다. 본 연구는 patS 유전자 주변의 DNA 염기 서열을 분석하여 codon 활용도를 알아 본 결과 patS를 제외한 다른 유전자가 존재하지 않음을 밝혔다. patS를 포함하는 세 개의 겹치는 cosmid를 찾아서 기존의 알려진 이형세포 발달 유전자를 탐색하였으나 나타나지 않았다. 질소원의 결핍에 반응하는 patS 유전자의 발현을 Northern blot 분석과 lacZ reporter 유전자 합성 실험을 통하여 각각 전사와 번역의 단계에서 알아보았다. patS 유전자의 발현은 배지로부터 질소원이 제거된 후 12시간 내에 급격히 증가하는 것으로 나타났다.

극지.고산식물 월귤의 격리 분포와 기온요인 (Disjunctive Distribution of Vaccinium vitis-idaea and Thermal Condition)

  • 공우석;임종환
    • 대한지리학회지
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    • 제43권4호
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    • pp.495-510
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    • 2008
  • 월귤은 극지 고산식물로 북극권과 고산대에 주로 자라며, 한반도에서는 북한의 고산대와 아고산대, 남한의 설악산 대청봉과 중청봉 사이 암석지, 귀때기청봉 등 아고산대 그리고 홍천의 산록에 자란다. 월귤이 격리 분포하는 강원도 홍천군 해발고도 350m 부근 애추 말단은 풍혈이다. 플라이스토세 빙하기에 널리 분포했던 월귤은 홀로세에 기후가 온난해지면서 한반도 북부와 중부의 고산대 및 아고산대와 함께 여름이 서늘한 홍천 풍혈 주위를 후빙기 피난처로 삼아 빙하기 유존종으로 격리 분포하게 되었다. 월귤의 남한계선이며 하한계선인 홍천에 자라는 군락은 가장 가까운 거리에 있는 설악산 아고산대와 1,200m의 해발고도 차이가 있으므로 빙하기의 기온은 현재보다 $6.6^{\circ}C$ 내외로 낮았던 것으로 추정된다 극지와 고산에 격리 분포하는 월귤은 여름 고온에 민감한 식물이다. 홍천의 산록에 자라는 월귤은 지구온난화에 따라 생리적으로 영향을 받거나 높은 온도를 좋아하는 식물과의 경쟁에 밀려 사라질 수도 있다. 한반도의 생물다양성 보전 차원에서 홍천의 월귤 군락지에 대한 보호 관리가 필요하다.

백제전통문양을 활용한 남성 풀오버 개발 (A design development of men's pullover based upon Baekje traditional patterns)

  • 서서영;박길순
    • 복식문화연구
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    • 제21권4호
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    • pp.494-510
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    • 2013
  • The purpose of this study was to develop unique men's pullovers using the patterns of Baekje tile, brick, and gilt-bronze shoes. As the method of the study, it was reviewed of the literature about Baekje traditional patterns and the knitting techniques. The development of pullover design was used TexPro design CAD program. The manufacture of pullover were knitted by Shima Seiki computerized knitting machines and Brother manual knitting machines. The results of study were as follows. First, the patterns of Baekje tile, brick and gilt-bronze shoes were suited to develop as motif for men's pullover. Second, men's pullover using Baekje traditional patterns were able to maintain the identity of Korean traditional culture and express the originality of design. Third, pullover was chosen three styles and was developed four design. Each pullover design was applied four colorway. Forth, pullover products were made by wool 100% yarn or cotton 47%/acrylic 53% mixed yarn. The patterns were expressed by knitting techniques of floating jacquard and birds-eye jacquard. Luxurious knit fashions were produced. Consequently, through the men's pullover development utilizing Baekje traditional patterns was showed the superiority of the local traditional culture, which is further emphasized by the current globalization. It also confirmed the possibility of the development of high value-added knitted fashion products to meet the needs of modern people to pursue individuality.

Assessment of Anthropometry, Nutritional Compositions and Contribution of School Meals to the Daily Nutrient Requirements of Primary School Children from Rural Communities

  • Ijarotimi O. Steve;Omotayo S. Amos
    • Journal of Community Nutrition
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    • 제8권4호
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    • pp.171-176
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    • 2006
  • The study aims to evaluate the nutritional status and influence of school meal intakes on RDA of primary school children in Akure community, Ondo State, Nigeria. A cross-sectional study was conducted among 728 primary school children aged between 6 and 15 years. Data were collected using interviewer-administered semi-structured questionnaires. The questionnaires collect information on demographic characteristics and home dietary intake of the subjects. The heights and weights of the children were measured using a standard procedure and height-far-age and weight-far-height z-score were determined. The children's school meal intakes were weighed for 4 days and samples were collected for chemical analysis. The results showed that 37.8% of the children were not wasted, 35.7% mildly wasted, 18.7% moderately wasted and 7.8% severely wasted. Also, 57.8% were not stunted, 29.3% mildly stunted, 11.0% moderately stunted and 1.9% were severely stunted. The subjects' home dietary intakes showed that 73.6% ate starchy food only, 19.9% ate protein based food, while 11.6% and 11.5% consumed fruits/vegetables and snacks to complement home meals respectively. The chemical composition of school meal was energy 379 - 413kcal, moisture content 5.9 -7.3g, carbohydrate 56.5 - 69.4g, fat 4.6 - 12.7g, crude fiber 0.1 - 2.4g, ash content 3.6 - 8.5g and protein 14.9 - 22.3g. The mineral contents were calcium 45.9 - 59.2mg, sodium 5004 - 59.6mg, zinc 2.3 - 3.1mg, magnesium 55.0 - 61.6mg, potassium 55.3 - 69.3mg, copper 0.2 - 0.3 mg, while others 1.3 - 1.9mg, 243 - 659mg and 831 - 9,510mg were iron, phosphorous and vitamin-A respectively. The contribution of school meals to subjects' RDA was within 2.9% and 1540%. In summary, school meal intake contributed positively to the RDA and nutritional status of the school children.

효소 첨가가 U.F. 치즈베이스 slurries의 숙성촉진에 미치는 영향 (Effects of Commercial Food Grade Enzyme on Acceleration of Ripening in U.F. Cheese Base Slurries)

  • 윤경;곽해수
    • 한국식품과학회지
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    • 제25권5호
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    • pp.510-516
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    • 1993
  • 이 실험에서 U.F.를 이용하여 제조한 치즈베이스의 숙성을 촉진시키기 위하여 시판되는 단백질 및 지방가수분해효소를 치즈베이스 slurries에 첨가하여 이화학적 특성을 조사하였다. 치즈베이스 slurries에서 TCA 가용성 질소화합물의 생성은 숙성 첫날에 급격한 증가를 보였고 그 후에는 완만했으며 체다치즈 slurries에서 보다 낮았다. PTA 가용성 아미노태 질소화합물의 생성도 TCA 가용성 질소화합물의 경향과 유사했지만 소량 생성되었다. 전기영동에서 치즈베이스 slurries와 체다치즈 slurries의 카제인이 모두 분해되었으나, 치즈베이스 slurries에서는 유청단백질이 거의 분해되지 않았다. 아미노산 생성에서 치즈베이스 slurries는 체다치즈 slurries에서 보다 매우 적은 함량을 보였다. 단쇄 유리지방산의 생성에서 치즈베이스 slurries는 체다치즈 slurries에서와 유사한 증가를 보였으며, 체다치즈의 자연숙성에서와 유사한 특이성을 나타내었다. 이 실험의 결과, 치즈베이스의 숙성 촉진을 위하여 효소첨가는 효과가 있는 것으로 나타났다.

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왕겨 연소기(燃燒機)를 이용(利用)한 온수(溫水)보일러 시스템 개발(開發) (I) -실험적(實驗的) 연구(硏究)- (Development of a Hot Water Boiler System with a Rice Hull Furnace)

  • 이용국;박승제;백풍기;노상하
    • Journal of Biosystems Engineering
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    • 제12권4호
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    • pp.31-43
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    • 1987
  • This study was performed to develop a hot water boiler system with small scale automatic rice hull furnace for the multi-purpose use in the farm. For the experiment a prototype hot water boiler system with rice hull furnace was fabricated, which was equipped with automatic hull feeder, igniter and ash removal device. Optimum operational conditions of the prototype: system were analyzed. The results arc summarized as follows. 1. The temperature measured right above the burning surface should be higher than $500^{\circ}C$ combustion. 2. The top zone of the combustion chamber was the most suitable location of the thermocouple to pick up the control temperature for the automatic operation of the rice hull furnace. 3. The content of carbon monoxide in the flue gas was increased with the filling height of burning material but it was less than 0.3 percent in volume in this experiment. When the filling height was expressed as the ratio of rice hull feed rate to the volume of the combustion chamber above the burning surface, the optimum ratio was about $150kg/m^3-h$. 4. The combustion efficiency of the prototype was higher than 95 percent when the feed rate was 1.1 to 2.3 kg/h and moisture content of rice hull was 22.4 percent (w.b.) or less. 5. It was estimated that the optimum operational conditions of the system were 1.3 to 2.0 kg/h in feed rate, 70 to 100 percent in excess air and 500 to $510^{\circ}C$ in control temperature. 6. The efficiency of coil heal exchanger increased with a decrease in feed rate of rice hull. When the rice hull feed rates were 1.1, 1.7 and 2.3 kg/h, the efficiencies of coil heat exchanger were about 34, 30 and 25 percent and heat transfer rates were 5.7, 7.6 and 8.8 MJ/h, respectively. When the flat plate heat exchanger was used in addition to the coil heat exchanger, the efficiency of the heat exchanger system increased to 48 percent.

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분자배열된 4,4',4''-tris(N-(1-naphthyl)-N-phenylamino)-triphenylamine 박막 제조와 전기적 특성 (Formation and Current-voltage Characteristics of Molecularly-ordered 4,4',4''-tris(N-(1-naphthyl)-N-phenylamino)-triphenylamine film)

  • 강도순;최영선
    • 공업화학
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    • 제18권5호
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    • pp.506-510
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    • 2007
  • 전기적 특성을 가지는 4,4',4''-tris(N-(1-naphthyl)-N-phenylamino)-triphenylamine (1-TNATA)가 유기발광소자(OLED)에서 전극으로 사용되는 ITO (Indium Tin Oxide)와 홀 수송층(Hole Transport Layer, HTL) 사이에 박막으로 진공증착되었다. 분자배열이 잘 되어진 1-TNATA의 경우 ITO와 홀 수송층 사이의 계면에서 생기는 전하주입장벽을 줄임으로 소자의 안정성과 효율을 높여준다. 본 연구에서의 라만 스펙트라(Raman spectra) 분석 결과, 증착된 1-TNATA 박막의 열처리와 증착하는 동안 전자기장 처리에 의해서 박막이 집적되고 분자배열이 이루어짐을 확인하였다. 열처리를 한 경우 1-TNATA 박막으로의 전류 흐름이 25% 증가하였다. 또한, $110^{\circ}C$에서 열처리한 1-TNATA 박막으로 제조된 다층유기발광소자의 전원 효율과 발광효율이 향상되었다. 열처리한 박막이 전자기장으로 처리한 박막에 비해 높은 효율을 나타내었다.

농촌주민의 건강상태와 영양지식.태도.실천과의 관계 (Relationship Nutritional Knowledge.Attitude.Practice and Health Status of Rural Residents)

  • 조유향;정해옥
    • 보건교육건강증진학회지
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    • 제23권1호
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    • pp.77-92
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    • 2006
  • Objectives: This study is to investigate and to test the overall level of nutritional knowledge. attitude. practice and health status of the residents in rural area. Methods: The interview survey was performed in March 2004 with structured questionnaires to 510 respondents of the residents who lived in Muan-Gun of Jeonnam province. The questionnaire was the abridged Scale of the Nutritional Knowledge Attitude Practice(SNKA) and health status as the morbidity, subjective health status, health management, and health examination. The covariate, F-test or t-test, and Chi-squire method were used for some of the cross-sectional data. Results: Average weight of nutritional knowledge was $10.64{\pm}5.98$ by SNKA of the 20 items(0-20). Average weight of nutritional attitude practice was $64.59{\pm}7.72$ by SNKA of the 20 items(20-80). The level of the nutritional knowledge was lower than the level of the nutritional attitude practice. 77.3% of the respondents have been health management, 49.6% of the respondents have been health examination. A majority of the respondents(31.3%) had disease, while 13.5% of the respondents had chronic disease, and 17.8% of the respondents had acute disease. The level of nutritional knowledge and general characteristics was positively correlated while the relationships were positive between nutritional knowledge and health status, and between nutritional attitude practice and health status. Conclusion: These results suggested that education programs of the nutritional knowledge were necessary for the residents of rural area. And behavior change of the nutritional knowledge were necessary for the residents of rural area. Further research would be required to specify the necessities and operation researches.

경유오염 토양의 생물정화공정에 대한 영양인자의 영향 분석 (Nutritional Factors Affecting Efficiency of a Bioremediation Process for Diesel-Contaminated Soil)

  • 노상철;이철효;장덕진
    • KSBB Journal
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    • 제14권4호
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    • pp.503-510
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    • 1999
  • 한국해양연구소에서 분양받은 유류분해효모 Yarrowia lipolytica CL180를 이용하여 경유로 오염된 토양에 대한 질소원, 인원 aeration rate, 그리고 균체량에 따른 영향을 조사하였다. 실험결과 질소원이 미생물 성장의 limiting factor로 작용하였으며, 다양한 비(C : N =100 : 5, 100 : 10, 100 : 15, 100 : 20 mg/kg soil)의 질소원을 첨가한 결과 C : N의 비(w/w)가 100 : 5일 때 가장 우수한 분해율과 균체수를 나타내었다. 질소원이 이 비율 이상으로 첨가되었을 때 분해율과 균체수가 낮게 나타났으며 이는 암모니아의 독성으로 인한 영향으로 사료된다. 그러나 인원과 통기에 따른 경유분해율의 변화는 없었으며 질소원이 첨가된 soil column에서는 7일이 경과된 후 약 50%의 경유잔류량을 나타내었다. 잔류경유를 제거하기 위하여 최초 접종량과 동일한 양의 균주를 접종하였으나 일정기간의 경과후에도 경유잔류량은 거의 변화가 없었다. 이는 경유의 토양 흡착 때문으로 사료된다.

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Effect of surface treatment methods on the shear bond strength of auto-polymerized resin to thermoplastic denture base polymer

  • Koodaryan, Roodabeh;Hafezeqoran, Ali
    • The Journal of Advanced Prosthodontics
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    • 제8권6호
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    • pp.504-510
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    • 2016
  • PURPOSE. Polyamide polymers do not provide sufficient bond strength to auto-polymerized resins for repairing fractured denture or replacing dislodged denture teeth. Limited treatment methods have been developed to improve the bond strength between auto-polymerized reline resins and polyamide denture base materials. The objective of the present study was to evaluate the effect of surface modification by acetic acid on surface characteristics and bond strength of reline resin to polyamide denture base. MATERIALS AND METHODS. 84 polyamide specimens were divided into three surface treatment groups (n=28): control (N), silica-coated (S), and acid-treated (A). Two different auto-polymerized reline resins GC and Triplex resins were bonded to the samples (subgroups T and G, respectively, n=14). The specimens were subjected to shear bond strength test after they were stored in distilled water for 1 week and thermo-cycled for 5000 cycles. Data were analyzed with independent t-test, two-way analysis of variance (ANOVA), and Tukey's post hoc multiple comparison test (${\alpha}=.05$). RESULTS. The bond strength values of A and S were significantly higher than those of N (P<.001 for both). However, statistically significant difference was not observed between group A and group S. According to the independent Student's t-test, the shear bond strength values of AT were significantly higher than those of AG (P<.001). CONCLUSION. The surface treatment of polyamide denture base materials with acetic acid may be an efficient and cost-effective method for increasing the shear bond strength to auto-polymerized reline resin.