KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지

Nutritional Factors Affecting Efficiency of a Bioremediation Process for Diesel-Contaminated Soil

경유오염 토양의 생물정화공정에 대한 영양인자의 영향 분석

  • Published : 1999.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

In order to analyze nutritional factors affecting in situ bioremediation of diesel degradation and cell viability were studied by varying nutritional conditions. In column experiments packed with diesel-contaminated soil, nitrogen was found to be the major limiting nutrient. When nitrogen was added to soil at four different levels of C : N (100 : 5, 100 : 10, 100 : 15, and 100 : 20 mg N/kg dry soil), the greatest simulation of microbial activity occurred at the lowest, rather than the highest nitrogen addition. However, no significant effects was observed when phosphorus and air were added. No matter how the incubation mode varied, less than 50% of the diesel was remained after 7 days of treatment, presumably because the residual hydrocarbons were adsorbed on soil particles, adsorption

한국해양연구소에서 분양받은 유류분해효모 Yarrowia lipolytica CL180를 이용하여 경유로 오염된 토양에 대한 질소원, 인원 aeration rate, 그리고 균체량에 따른 영향을 조사하였다. 실험결과 질소원이 미생물 성장의 limiting factor로 작용하였으며, 다양한 비(C : N =100 : 5, 100 : 10, 100 : 15, 100 : 20 mg/kg soil)의 질소원을 첨가한 결과 C : N의 비(w/w)가 100 : 5일 때 가장 우수한 분해율과 균체수를 나타내었다. 질소원이 이 비율 이상으로 첨가되었을 때 분해율과 균체수가 낮게 나타났으며 이는 암모니아의 독성으로 인한 영향으로 사료된다. 그러나 인원과 통기에 따른 경유분해율의 변화는 없었으며 질소원이 첨가된 soil column에서는 7일이 경과된 후 약 50%의 경유잔류량을 나타내었다. 잔류경유를 제거하기 위하여 최초 접종량과 동일한 양의 균주를 접종하였으나 일정기간의 경과후에도 경유잔류량은 거의 변화가 없었다. 이는 경유의 토양 흡착 때문으로 사료된다.

Keywords

References

  1. 미생물계면활성제를 이용한 오염토양 복원기법과 현장적용성에 관한 연구 송태을;상병인;김만호;황종식
  2. 석유와 윤활 v.11 경유의 품질특성에 관한 조사연구 석유와 윤활社 조사연구과
  3. J. Air Waste Manage. Assoc. v.41 Innovative Technologies for Contaminated Site Remediation: Focus on Bioremediation Gabriel, P. F.
  4. Intrinsic Bioremediation Enhanced Aerobic Bioremediation of Residual Hydrocarbon Sources MaAllister, P. M.;C. Y. Chiang;J. P. Salanitro;I. J. Dortch;P. Williams;R. E. Hinchee(ed.);J. T. Wilson(ed.);D. C. Downey(ed.)
  5. Bioaugmentation for Site Remediation Bioremediation: When is augmentation needed? Forsyth, J. V.;Y. M. Tsao;R. D. Bleam;R. E. Hinchee(ed.);J. T. Wilson(ed.);D. C. Downey(ed.)
  6. 한국미생물학회지 v.32 토양미생물군집의 개체수와 활성도에 미치는 경유의 영향 서은영;송홍규
  7. Appl. Microbiol. Biotechnol. v.49 Microbial Degradation of Hydrocarbons in Soil during Aerobic/anaerobic changed and under purely Aerobic Conditions Loser, C.;H. Seidel;A. Zehnsdorf;U. Stottmeister
  8. Appl. Environ. Microbiol. v.63 Efficiency of Indigenous and Inoculated Cold-Adapted Soil Microorganisms for Biodegradation of Diesel Oil in Alpine Soils Margesin, R.;F. Schinner
  9. Appl. Microbiol. Biotechnol. v.47 Bioremediation of Diesel-oil-contaminated Alpine Soils at Low Temperatures Margesin, R.;F. Schinner
  10. Appl. Environ. Microbiol. v.62 Development of Fungal Inocular for Bioaugmentation of Contaminated Soils Lestan, D.;R. T. Lamar
  11. 한국생물공학회지 v.13 Hydrocarbon Uptake Modes에 따른 유류분해 미생물 혼합제의 원유분해능 고성환;이홍금;김상진
  12. Guidelines Establishing Test Procedures for the Analysis of Pollutants under the Clean Water Act : Final Rule and Interim Final Rule and Proposed Rule v.40 U. S. EPA
  13. EPA 600/4-84-027 Interlaboratory Comparison Study: Methods for Volatile and Semi-Volatile Compound U. S. EPA
  14. Appl. Environ. Microbiol. v.61 Effects of Environmental Parameters on the Formation and Turnover of Acetate by Forest Soils Kirsten K.;H. L. Drake
  15. Environ. Toxicol. Chem. v.14 Biodegradation of No. 2 Diesel Fuel in the Vadose Zone: A Soil Column Study Widrig, David. L.;John F. Manning, Jr.
  16. Biores. Technol. v.59 In Situ Bioremediation of Explosives Contaminated Soil: A Soil Column Study Boopathy, R.;D. L. Widrig;J. F. Manning
  17. Standard Methods for the Examination of Water and Wastewater(19th ed.) Waton, A. D.;L. S. Clesceri;A. E. Greenberg
  18. Standard Methods for the Examination of Water and Wastewater(19th ed.) Waton, A. D.;L. S. Clesceri;A. E. Greenberg
  19. Microbiol. Mol. Biol. v.61 Microbial Production of Surfactants and Their Commerial Potential Desai, J. D.;I. M. Banat
  20. Environ. Sci. v.31 Enhancement and Inhibitor of Microbial Activity in Hydrocarbon-Contaminated Arctic Soils: Implications for Nutrient-Amended Bioremediation Braddock, Joan F.;Marian L. Ruth;Peter H. Caterall
  21. EPA 510-B-95-007 How to Evaluate Alternative Cleanup Technologies for Underground Storage Tank Sites: A Guide for Corrective Action Plan Reviewers U. S. EPA
  22. Chem. Eng. Prog. no.February Consider Bioremediation for Waste Site Cleanup Bradford, M. L.;R. Krishnamoorthy
  23. Appl. Environ. Microbiol. v.65 Bacterial Adhesion to Soil Contaminants in the Presence of Surfactants Stelmack, P. L.;M. R. Gray;M. A. Pickard
  24. Environ. Sci. Technol. v.31 Formation and Removal of Hydrocarbon Residual in Porous Media: Effects of Attached Bacteria and Biosurfactants Heman, D. C.;R. J. Lenhard;R. M. Miller
  25. J. Industrial Microbiol. v.16 Environmental Application of Immobilized Microbial Cells: a Review Cassidy, MB;H. Lee;J. T. Trevor