• Title/Summary/Keyword: 5세대

Search Result 2,477, Processing Time 0.027 seconds

Distribution of Habitats and Ecology of Weedy Melons (Cucumis melo var. agrestis Naud.) in Korea (우리나라 야생잡초 참외의 자생지 분포지역 및 생태)

  • Lee, Woo-Sung
    • Horticultural Science & Technology
    • /
    • v.31 no.5
    • /
    • pp.652-655
    • /
    • 2013
  • Natural habitats of weedy melons were distributed on the islands along and on the west and south coasts of Korean peninsula including Boryeong, Seosan (Taean), Seocheon, Okgu, Buan, Gochang, Yeonggwang, Muan, Shinan, Haenam, Jindo, Wando, Goheung, Yeocheon, Hadong, Namhae, Goseong, Tongyeong, Geoje, and Jeju islands including Jeju city, Bukjeju-gun and Nam Jeju-gun. Weedy melons were found growing wildly in or around the cultivated lands in these regions. Natural habitats of weedy melons were in and around the cultivated lands. Weedy melon plants were found most often in soybean fields, followed by fields of mungbean, sweet potato, pepper, sesame, cotton, and peanuts. The plants were also found growing wild in foxtail millet fields, rice paddy levees along the streams, upland field edges, watermelon fields, corn fields, vegetable gardens near farmhouse, orange fields, compost piles, fallow fields, roadside and home gardens. They inhabited in sunny and a little dry spaces in relatively low-height crop plant fields in general. The time of fruit maturity was from early July to late October with the most frequency in September according to post survey answer. Fruits dropped off from the fruit stalk when matured. This phenomenon was thought beneficial for perpetuation in the wild. The fruits were being used commonly for food and toys for children. It was thought that weedy melons were perpetuating through the cycle of human and animal feeding of the fruits, human and animal droppings, often mixed in compost, and application of the compost to crop fields by human.

Detection of the Factors Related to spermatization in Sclerotinia trifoliorum -I. Course of Fertilization (Sclerotinia trifoliorum의 Spermatization에 관여하는 요인(要因)의 검색(檢索) -I. 균(菌)의 수정과정(受精過程))

  • Uhm, Jae Youl;Kim, Young Tae
    • Current Research on Agriculture and Life Sciences
    • /
    • v.5
    • /
    • pp.127-133
    • /
    • 1987
  • The process of fertilization and changes in anatomical structure of sclerotia during the apothecial formation in Sclerotinia trifoliorum, the causal fungus of sclerotial rot of forage legumes, were investigated. The time of fertilization could be estimated with fair accuracy by the sequencial spermatization of the sclerotia which kept at 15C in saturated moisture. In the case of one strain used in this experiment, fertilization between the sclerotia and spermatia were estimated to take place at around 18days after the sclerotia were placed under the conditions for apothecial induction (15C, saturated moisture). The fertilizable state was maintained for about 45 days and the spermatization thereafter did not induce the apothecial formation. When the sclerotia reached fertilizable state, a number of interwoven hyphal nests were developed within the medulla of sclerotia, regardless of the sexuality of the cultures. Comparing the process of multiplication and growth of the hyphal nests in homothallic and heterothallic culture, they were identified as ascogonium. These ascogonia were persisted for about 45 days. This observation was well coincided with the duration of fertilizable state elucidated by the sequencial spermatization experiment.

  • PDF

A New Soybean Cultivar 'Daewang' with High Quality and Large Seed Size (장류용 고품질, 대립 콩 신품종 '대왕')

  • Choi, Jae-Keun;Ha, Keon-Soo;Byun, Hak-Soo;Heo, Nam-Ki;Jang, Eun-Ha;Kim, Sang-Soo;Lee, Se-Jong;Sa, Jong-Goo
    • Korean Journal of Breeding Science
    • /
    • v.43 no.3
    • /
    • pp.177-179
    • /
    • 2011
  • A new soybean cultivar 'Daewang' was released by Agricultural Research & Extension Services, Gangwon Province in 2008. The Goals of breeding the variety was for large seed size, high yield, lodging tolerance and resistance to diseases such as soybean mosaic virus (SMV) and bacterial pustule. 'Daewang' was derived from the cross of GWS91, which has yellow seed coat, large seed size and late maturity, and 'Seokryang-putkong', which has large seed size and early maturity. The preliminary, advanced and regional yield trials for evaluation and selection were carried out from 2002 to 2007. It has a determinate growth habit with purple flower, yellow seed coat, yellow hilum and large seed weight (33.6 g per 100 seeds). The maturity date of 'Daewang' is 16 days later than the 'Taekwang'. It has a high content of total isoflavone ($1,851{\mu}g/g$). The average yield of 'Daewang' was 2.68 MT/ha in the regional yield trials (RYT) carried out in four locations in Gangwon province from 2004 to 2007 which was 4 percent higher than the check cultivar 'Taekwang'.

Efficient Transformation Method of Soybean Using Meristematic Tissues of Germinating Seeds (발아종자의 분열조직을 이용한 효율적인 콩 형질전환 방법)

  • Kim, Yul-Ho;Park, Hyang-Mi;Choi, Man-Soo;Sohn, Soo-In;Shin, Dong-Bum;Lee, Jang-Yong
    • Korean Journal of Breeding Science
    • /
    • v.40 no.3
    • /
    • pp.278-285
    • /
    • 2008
  • An efficient transformation method for soybean [Glycine max (L.) Merr.] using meristematic tissues of germinating seeds has been established. The embryonic axes were excised from germinating seeds of Korean soybean cultivar, Iksannamulkong and 0.5-2 cm long segment containing meristematic tissues were prepared by cutting hypocotyl region. The explants were inoculated with Agrobacterium tumefaciens strain LBA4404 harboring a binary vector with the bar gene as a selectable marker gene and a ${\beta}-glucuronidase$ (GUSINT) reporter gene, and then co-cultured for 7 days on co-cultivation medium (CCM). The meristematic tissues were cultured on shoot induction medium (SIMP6) supplemented with 0.4 mg/l $N_6-benzylaminopurine$ (BAP) and 0.1 mg/l indolebutyric acid (IBA) in the presence of 6 mg/l L-phosphinotricin (PPT) for 2 weeks and the surviving explants were transferred to shoot elongation medium (SEMP6). Transformation was confirmed by Southern blot analysis and the transformation efficiencies ranged from 1.48 to 2.07%. The new modified transformation method was successfully implemented for obtaining several transgenic lines with SMV-CP gene. It is expected that this method could efficiently be used for the transformation of recalcitrant soybean cultivars.

A New Orange Mini Gerbera hybrida 'Yetty' with High Yield for Cut Flower (오렌지색 소륜 다수성 절화용 거베라 신품종 '예티' 육성)

  • Chung, Yong Mo;Lee, Byeong Jeong;Hwang, Ju Chean;Chin, Young Don;Kim, Su Kyeong;Yi, Young Byung;Kwon, Oh Chang
    • FLOWER RESEARCH JOURNAL
    • /
    • v.17 no.3
    • /
    • pp.201-204
    • /
    • 2009
  • A new orange mini gerbera (Gerbera hybrida Hort.) 'Yetty' was developed from a cross 'Haiti' and 'Nova Zembra' followed by seedling and line selections at the Flower Research Institute, Gyeongnam Agricultural Research and Extension Services (ARES) from 2001 to 2006. Three times evaluations were conducted from 2004 for the detailed characteristics of the new cultivar, 'Yetty' has single type mini flower in good harmony with orange (RHS 25-A) ray floret and browen center. It has good stable flower shape and strong peduncle, and its vase life was 14.8 days. The average yield of 'Yerty' was 98.5 flowers per plant a year in greenhouse yield trials carried out from 2004 to 2006. This cultivar registered for commercialization in 2007. 'Yerty' would be cultured under greenhouse condition in Korea.

Sustainable Urban Regeneration and Smart Water Management (지속가능한 도시재생과 스마트 물 관리)

  • Lee, Yoo Kyung;Lee, Seung Ho
    • Proceedings of the Korea Water Resources Association Conference
    • /
    • 2018.05a
    • /
    • pp.86-86
    • /
    • 2018
  • 본 연구는 한국의 도시재생과 스마트 물 관리의 정책 분석을 위하여 도시재생과 스마트 물 관리의 등장 배경, 주요 현안 및 연계성을 모색하고 도시재생방안으로서 스마트 물 관리체계의 가능성을 검토하는 것을 목적으로 한다. 1950년대의 도시재건(Urban Reconstruction)과 1970~80년대의 도시재개발(Urban Renewal, Urban Redevelopment) 등의 정비 사업은 물리적 환경정비에 초점을 맞추었다. 그러나 1990년대 환경문제가 세계적 이슈로 등장하면서 교외지역 난개발 문제에 대한 대응책이 필요하게 되었고 도시의 물리 환경적, 산업 경제적, 사회 문화적 측면을 부흥시키는 도시재생 접근법이 출현하였다. 한국 정부는 2017년부터 시작한 '도시재생 뉴딜사업'의 일환으로 스마트 기술을 적용한 도시재생사업을 통해 스마트도시 선도국가 도약과 세계적 흐름에 부합하는 도시성장을 기대하고 있다. 1980년대 초 등장한 스마트 기술은 2000년대 들어와 스마트 도시, 스마트 인프라, 스마트 그리드 등의 분야로 확대, 진보하였다. 물 분야의 스마트 기술은 2009년 스마트워터그리드 이니셔티브(Smart Water Grid Initiative)의 발족과 함께 IBM, CISCO, Intel 등의 IT 기반 물 관리 워킹그룹 형성, Suez, Veolia, Siemens 등 수처리 기업의 스마트워터그리드 분야 진출 모색과 함께 발전하기 시작하였다. 이후 2012년 유엔 스마트 물 관리 포커스 그룹(ITU-T SG 5)의 스마트 물 관리 표준화 연구가 착수되었고 한국은 국토교통부 건설교통기술 연구 개발사업 중 하나로 스마트 물 관리 장기 연구 사업을 시작하였다. 스마트 물 관리는 수자원 및 상하수도 관리의 효율성 제고를 위하여 스마트 미터, 센서, 디지털지도제작 등 ICT를 이용한 차세대 물 관리시스템이라고 정의할 수 있다. 구체적인 대상 분야를 고려한다면 하천수, 우수, 지하수, 하폐수처리수, 해수담수 등 다양한 수자원의 관리, 물의 생산과 수송, 사용한 물의 처리 및 재이용 등 물 관리 전 분야를 포함한다. 그러나 스마트 물 관리의 용어와 개념을 처음으로 도입한 미국 등 선진국과 관련기업들은 스마트 물 관리를 '스마트 워터 미터, 센서, 첨단 모델링, 수문 지도제작, 스마트 관개농업, 자동화 로봇 등 다양한 기술을 통합적으로 운영하는 지능적인 수자원 관리를 위한 정보네트워크'로 정의한다. 일찍이 도시재생으로의 패러다임 전환을 실시한 영국 및 일본과 달리 한국의 도시재생은 개념, 구성요소, 범위, 사업방식 등의 여러 가지 측면에서 아직 형성단계에 있다. 또한 한국의 스마트 물 관리 논의는 개념정립 측면에서 심층적 논의가 거의 부재하였다. 기존의 논의들은 수자원 혹은 상하수도서비스 분야에서의 연구결과와 기술개발성과를 기계적으로 적용하고 확대하는 측면만을 부각시켰다. 그러나 이와 같은 스마트 물 관리에 대한 논의는 정보통신기술과 물 관리 서비스를 단편적으로 연결하고 적용범위를 제한할 수도 있다는 점에서 한계성이 있다. 본 연구는 국내외 문헌검토를 바탕으로 한국의 도시재생과 스마트 물 관리의 정책을 분석하고 지금까지 별개로 간주된 두 개념의 장점을 융합하여 향후 지속가능한 도시개발 사업으로서의 가능성을 검토하고자 한다.

  • PDF

Application of mass-spectrometry compatible photocleavable surfactant for next-generation proteomics using rice leaves (벼의 차세대 단백질체 분석을 위한 질량분석기 호환의 광분해성 계면활성제의 적용)

  • Shin, Hye Won;Nguyen, Truong Van;Jung, Ju Young;Lee, Gi Hyun;Jang, Jeong Woo;Yoon, Jinmi;Gupta, Ravi;Kim, Sun Tae;Min, Cheol Woo
    • Journal of Plant Biotechnology
    • /
    • v.48 no.3
    • /
    • pp.165-172
    • /
    • 2021
  • The solubilization of isolated proteins into the adequate buffer containing of surfactants is primary step for proteomic analysis. Particularly, sodium dodecyl sulfate (SDS) is the most widely used surfactant, however, it is not compatible with mass spectrometry (MS). Therefore, it must be removed prior to MS analysis through rigorous washing, which eventually results in inevitable protein loss. Recently, photocleavable surfactant, 4-hexylphenylazosulfonate (Azo), was reported which can be easily degraded by UV irradiation and is compatible with MS during proteomic approach using animal tissues. In this study, we employed comparative label-free proteomic analysis for evaluating the solubilization efficacies of the Azo and SDS surfactants using rice leave proteins. This approach led to identification of 3,365 proteins of which 682 proteins were determined as significantly modulated. Further, according to the subcellular localization prediction in SDS and Azo, proteins localized in the chloroplast were the major organelle accounting for 64% of the total organelle in the SDS sample, while only 37.5% of organelle proteins solubilized in the Azo were predicted to be localized in chloroplast. Taken together, this study validates the efficient solubilization of total protein isolated from plant material for bottom-up proteomics. Azo surfactant is suitable as substitute of SDS and promising for bottom-up proteomics as it facilitates robust protein extraction, rapid washing step during enzymatic digestion, and MS analysis.

Development of Salt-Tolerant Transgenic Rice Using Soybean PR10 Gene (콩의 Pathogenesis-Related 10 유전자를 이용한 내염성 벼 형질전환 계통 개발)

  • Kim, Hyo Jin;Baek, So Hyeon;Shin, Woon Chul;Seo, Chun Sun;Park, Myoung Ryoul;Ko, Jae Kwon;Yun, Song Joong
    • Korean Journal of Breeding Science
    • /
    • v.42 no.5
    • /
    • pp.540-546
    • /
    • 2010
  • This study was conducted to understand the role of soybean pathogenesis-related 10 (GmPR10) gene in salt tolerance and to develop salt-tolerant rice using GmPR10 cDNA. GmPR10 transgene was expressed constitutively in the shoot and root of the $T_1$ transgenic rice plants. Interestingly, however, the levels of the transgene expression were increased temporally up to over four- to five-fold in the shoot and root by 125 mM NaCl treatment, peaking at six hours after the treatment and decreasing thereafter. Electrolyte leakage of leaf cells under 125 mM NaCl treatment was lower in all the transgenic lines than in the control variety, Dongjin-byeo. Ability of seedlings to recover from 125 mM NaCl treatment for two weeks was higher in the transgenic plants than in the control plants. These results demonstrated that GmPR10 had function to increase cell integrity and promote growth under the saline stress imposed by NaCl. The transgenic line GmPR10-3 which showed highest ability to recover from the saline stress could be used as a potential source for salt tolerance in rice breeding programs.

Development of Selectable Marker of High Oleate Trait in Peanut (Arachis hypogaea L.) (땅콩에서 고 올레인산 형질관련 분자마커의 선발)

  • Yang, Kiwoung;Pae, Suk-Bok;Park, Chang-Hwan;Lee, Myoung Hee;Jung, Chan-Sik;Son, Jeong-Hee;Park, Keum-Yong
    • Korean Journal of Breeding Science
    • /
    • v.42 no.5
    • /
    • pp.507-514
    • /
    • 2010
  • Peanut(Arachis hypogaea L.) is one of the major oilseed crops. The peanut oil consists of palmitic, oleic and linoleic acids, which are present at levels of 10%, 36-67% and 15-43%, respectively. High oleate mutant of peanut F435 contains 80% oleate and as little as 2% linoleate in seed oil. Previous study indicated that delta 12 fatty acid desaturase is a major enzyme controlling the oleate content in seeds of oilseed crops. F435 sequence alignment of their coding regions disclosed that an extra A(adenine) was inserted at the position +2,823 bp of delta 12 fatty acid desaturase gene. This study was to develop molecular marker (SNP marker) co-segregating with the high oleate trait. Chopyeong ${\times}$ F435 $F_2$ 41 population were investigated using molecular marker and fatty acid assay (NIR and gas chromatography). Finally, this marker segregates Chopyeong type 26 lines, heterotype 9 lines and F435 type 6 lines. These results in our study suggested that SNP marker conform fatty acid assay.

Relationship between Polyphenol Oxidase Activity and Color of White Salted Noodles Prepared from Korean Wheat Cultivar (국산밀의 폴리페놀 산화 효소 활성과 국수 색과의 관계)

  • Kang, Chon-Sik;Kim, Kyeong-Hoon;Park, Jong-Chul;Kim, Kyung-Ho;Park, Kwang-Geun;Cheong, Young-Keun;Yoon, Sung-Joong;Park, Chul Soo
    • Korean Journal of Breeding Science
    • /
    • v.43 no.5
    • /
    • pp.470-478
    • /
    • 2011
  • This study was carried out to find major factors affecting the color of wheat flour and noodles and develop simple analysis method for the breeding of wheat cultivars suitable for producing flour and noodles with bright color which are preferred by consumers. Customers who buy white noodle or flour prefer bright-colored food to dark-colored products. We evaluated grains of 25 Korean wheat cultivars for chemical composition, grain characteristics, and color change of noodle dough sheets during storage. Polyphenol oxidase (PPO) has been connected to discoloration of white salted noodles and other wheat end products. The grain PPO activity was reduced as the 1,000 grain weight, grain ash content, and protein content decreased. The grain PPO activity was positively correlated with the total polyphenol content ($r=0.609^{**}$) and iron content ($r=0.655^{**}$). Lightness, protein, polyphenol and Fe content of flour were positively correlated with PPO activity of grain. Cultivars with higher grain PPO activity showed darker noodles and were more easily discolored during the storage (from 2 hr to 48 hr). Thus, PPO activity can be used as a selection index in a breeding program for wheat cultivars of bright-colored flour.