• Maxillofacial Plastic and Reconstructive Surgery
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• 제16권3호
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• pp.321-347
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• 1994

교근의 근방추에서 유래하여 단일 연접성 고유수용성 악반사에 관여하는 구심성 신경섬유를 단일 축삭내 HRP 주입기법으로 염색해서 삼차신경 운동핵에서의 type I a 및 type II 신경 섬유 종말부의 미세 구조 및 연접양상을 분석한 결과는 다음과 같았다. ${\cdot}$ 대부분의 표식 종말지는 dome, 길쭉한 형태 혹은 둥근 모양을 나타내었으며, scalloped 형태 혹은 glomerulus 형태는 관찰되지 않았다. ${\cdot}$ 표식 종말지는 다수의 균일한 형태 및 크기(49-52nm) 의 투명한 구형의 소포와 때때로 소수의 치밀소포(직경:87-114nm)를 함유하고 있었다. ${\cdot}$ 표식 종말지의 크기는 type I a가 장경($1.91{\pm}0.63{\mu}m$), 단경($0.94{\pm}0.28{\mu}m$) 및 평균 직경($1.42{\pm}0.40{\mu}m$)에서 type II보다 작았다(p<0.05). ${\cdot}$ 표식 종말지는 신경원체 혹은 가지돌기와 비대칭 연접을 이루었으며, 다형소포와 같은 연접소포들을 함유하는 종말부와의 연접은 관찰되지 않았다. ${\cdot}$ 대부분의 type I a (94.9%) 및 type II(85.9%) 종말지는 단지 1개의 neuronal propile과 연접을 이루었으며, 4개 이상의 neuronal propile들과 연접을 이루는 경우는 관찰되지 않았다. ${\cdot}$ type I a 종말지에 있어서 대부분(87.2%)은 신경원체 및 근심부 가지돌기와 연접을 이루는데 반해 type II 종말지는 다수가(64.8%) 원심부 가지돌기와 연접을 이루었으며 신경원체 및 근심부 가지돌기와 연접을 이루는 경우는 35.2%에 그쳤다. 이상을 종합해 보면 단일 연접성 악반사를 일으키는 구심성 신경섬유 종말부는 삼차운동신경핵에서, 동통 및 촉각 또는 감각분별등 복잡한 감각을 처리하는 감각핵에서 보다 연접후신경원에 강력한 흥분성 신호를 전달하며 또한 대단히 단순한 연접양상을 이루는 것으로 나타났다.

• 한국중재학회지:중재연구
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• 제24권4호
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• pp.49-97
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• 2014

This study is about the availability of Chinese internal arbitration institutions by Korean invested companies. Generally, Chinese internal arbitration institutions lack independence from government. However, because parties seeking an arbitration award have ways to get neutrality from internal arbitration institutions that guarantee party autonomy, these Korean companies can use Chinese internal arbitration institutions to resolve disputes in China. Special attention should be given to the following. First, because Korean companies invested in China are legally in the same position as Chinese companies, unless foreign-related factors intervene, when disputes occur with Chinese companies or individuals, the disputes correspond to internal dispute, and when it comes to choosing the arbitration institution, these Korean companies must choose either a Chinese internal arbitration institution or foreign-related arbitration institution. Second, most Chinese internal arbitration institutions still lack independence from government, which can influence the fairness of arbitration in the future. Therefore, Korean companies invested in China should think about alternative ways to get a minimum impartiality in arbitration cases. Third, the parties are allowed to choose arbitration rules freely in Beijing, Xian, Chongqing, Guangzhou, and Hangzhou arbitration commissions. Therefore, in arbitration cases, the parties can get impartiality by choosing arbitrators according to the arbitration rules which they agree on, or by choosing partially modified arbitration rules of those arbitration commissions. Fourth, in order to get an impartial arbitration award from Chinese internal arbitration institutions in China, it is important for Korean lawyers or arbitration experts -- fluent in Chinese -- to be registered in the List of Arbitrators of Chinese internal arbitration institution by way of signing a MOU between the Korean Commercial Arbitration Board, or the Korean Association of Arbitration Studies and arbitration commissions such as those of Beijing, Xian, Chongqing, Guangzhou, and Hangzhou which comparatively do guarantee party autonomy. Fifth, because application of the preservation of property before application of arbitration is not approved in China, in practice, in order to preserve property before application of arbitration, it is best to file another suit in China based on other legal issue (e.g., tort) independent from the contract which an arbitration agreement is applied to. Sixth, in arbitration commissions which allow different agreement regarding arbitration procedures or arbitration rules, it is possible to choose a neutral arbitrator from a third country as a presiding arbitrator via UNCITRAL arbitration rules or ICC arbitration rules. Seventh, in the case of Chinese internal arbitral award, because the court reviews the substantive matters to decide the refusal of compulsory execution, the execution rate could be relatively lower than that of foreign-related cases. Therefore, when Korean companies invested in China use Chinese internal arbitration institution, they should endure low rate of execution. Eighth, considering the operational experiences of public policy on foreign-related arbitration awards so far, in cases of Chinese internal arbitration award, the possibility of cancellation of arbitral award or the possibility to refuse to execute the award due to public policy is thought to be higher than that of foreign arbitral awards. Ninth, even though a treaty on judicial assistance in civil and commercial matters has been signed between Korea and China, and it includes a provision on acknowledgement and enforcement of arbitral award, when trying to resolve disputes through Chinese internal arbitration institution, the treaty would not be a big help to resolve the disputes, because the disputes between Korean companies invested in China and the party in China are not subject to the treaty. Tenth, considering recent tendency of conciliation by the arbitral tribunal in China and the voluntary execution rate of the parties, the system of conciliation by the arbitral tribunal is expected to affect as a positive factor the Korean companies that use Chinese internal arbitration institution. Finally, when using online arbitration, arbitration fees can be reduced, and if the arbitration commissions guaranteeing party autonomy have online arbitration system, the possibility of getting impartial arbitration award through them is higher. Therefore, the use of online arbitration system is recommended.

• 한국학교보건학회지
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• 제8권2호
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• pp.233-243
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• 1995

A survey has conducted on two hundred high school students of the first and second grade by way of questionnaine in Seoul. The purpose of this study on the information from the survey is to cstimate the level of awareness and probe how they feel and what they wish on sex. X2 inspection is designed to assess general aspects of responded contents by way of percentage and examine degree of satisfaction on previously experienced sex education and demand for sex education. The result is as follows. 1. As a result of the examination of degree of awareness on sex of respondents, 62% delines sex as human relationship including moral values for harmony between men and women. 64% of the men and 70% of the women say that sex is a natural thing. It comes to the conclusion that most of the respondent sgenerally consider sex positive. On chastity, 68.5% of the respondent answer that it means physical chastity, 12.5% that chastity before marriage should be kept, and 43% that it should be kept as far as possible. Most of them pespond that it should be kept. 2. As a result of the examination on the contents of sex, they answer they know well in the order of masturbation(72%), pregnancy(76%), and sex(63%). Contraception, abortion and ejection are contents they poorly informed of. 3. As a result of the examination on experienede of sex education, 83% of men and 100% of women have experienced sex, education, but their degreeof satisfaction to it is very low. And 49% of the whole is dissatisfactory to it. There is some difference between male and female students.(p=0.000) That result translates that female studeuts are educated on sex tjhrough more systematic subjects than male ones. In addition, it turns out that teenagers get most information on sex through friends, seniors, and mass media such as videos, TV and radios. Correct and systematic sex education is need because wrong information on sex culd be taught and bring them to misbehave. 4. 87.5% of the respondents answer that sex education is necessary, so that degree of necessity for sex education, turns out to be very high. Also the main subject that should perform sex education is in the order of school(50%), Society(24.5), home(18.5%). They respond that most appropriated period for the beginning of sex education is about elementary school age(43%), and 34% believes it to be put ahcad of elementary school age. Anurse teacher accounts for 54% for the main subject that addresses sex education, to male students visit teachers are most favored for 50%. As a result of those, it could be concluded that most high school students want sex education from responsible persons who have systematic and professional knowledge on sex. 5. In order to perform proper sex education by the above results, better educational effects are obtained when characteristics and natures of teenagers are known and most wanted knowledge by them is taught in priority in times of planning sex education. Besides, the contents of sex education suitable for each school should be planned before elementary school age and sex education should be performed in accordance with students' demand. In addition, sex education should be attentively performed by home, all organizations of society as well as school. Therefore, sex education will play a great role in making teenagers reestablish their conception on sex when the traditional and moral value systems of our country and the new value systems which are being formed under the influence of the western culture are in harmony.

• 한국초지조사료학회지
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• 제38권2호
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• pp.106-111
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• 2018

본 연구는 고창증 발병으로 폐사된 저지종 젖소의 반추위액과 캐놀라가 장착한 저지종 젖소의 반추위액을 샘플링하여 반추위 내 미생물 균총의 급작스런 변화에 따른 반추위 발효와 고창증 발병간에 연관성을 알아보고자 진행되었다. 채취한 반추위액 샘플은 PowerMax Soil DNA Isolation Kit (MO BIO Inc., CA, USA)를 이용하여 DNA를 추출한 후 PicoGreen (Turner BioSystems, Inc., CA, USA)과 Nanodrop spectrophotometer (ND-1000, NanoDrop Technologies Inc., DE, USA)를 이용하여 260/280 nm와 260/230 nm 흡광도 값을 측정하였다. DNA를 정량분석하고 $MiSeq^{TM}$ platform (Illumina, CA, USA)을 이용한 장내 미생물 균총의 다양성 분석을 마크로젠(Macrogen Inc., Seoul)에 의뢰하여 실시하였다. 반추위액 내 미생물 균총 분석 결과, 전분 분해균의 경우 고창증 발병으로 인한 폐사우의 반추위 내에서 R. bromii가 우점 하였으며, R. bromii, B. pseudolongum, B. merycicum 및 B. fibrisolvens 등과 같은 전분 분해균들이 정상우 대비 36배 높았다. 반면 반추위 발효와 밀접한 관련이 있는 F. succinogenes, R. albus 및 R. flavefaciens 등과 같은 대표적인 섬유소 분해균 비율은 정상우에서 고창증 발병으로 인한 폐사우 대비 12배 높았다. 이와 같은 결과를 볼 때, 반추위 내 균총 가운데 R. bromii, B. pseudolongum, B. merycicum 및 B. fibrisolvens 등과 같은 전분 분해균들의 급격한 증가가 반추위 내 pH 저하 및 가스 생성 증가를 초래했고 이는 저지종 젖소의 고창증 발병으로 인한 폐사와 밀접한 연관이 있었을 것으로 판단된다.

• Restorative Dentistry and Endodontics
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• 제27권6호
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• pp.577-586
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• 2002

본 연구는 엔진구동형 Ni-Ti 합금 파일의 삭제날 각의 차이가 근관 성형 후 근관 내 절삭 면에 형성되는 도말층의 분포, 형태 및 상아세관 내로의 침투 정도 등에 어떤 차이를 보이는가를 알아보기 위함이다. 건전한 36개의 단근관을 갖는 발치된 사람의 단근치를 선택하고 positive cutting angle을 갖는 HEROShaper 파일과 negative cutting angle로 된 ProFile 파일을 이용하여 근관성형 후 절단면에서의 도말층의 양, 분포 형태 및 상아세관내로의 침투 빈도를 주사전자 현미경을 이용하여 사진촬영 후 다음과 같은 결과를 얻었다. 1. 사용된 엔진구동형 합금 파일의 삭제날 각의 차이에 관계없이 모든 군에서 도말층이 형성되었다. 2. 근관 성형 후 절삭면상에 나타난 도말층 형태는 삭제날 각의 차이에 따라 형태학적인 차이를 보였으며, positive cutting angle과 radial land가 없는 니켈-티타늄 전동통파일에서 상대적으로 적은 양의 도말층 형성을 보였다(p < 0.05). 결론적으로, 본 연구는 삭제날의 각이나 radial land의 유무에 관계없이 사용된 모든 엔진구동형 파일에서 도말층이 형성된 것으로 미루어 근관 성형 시 liquid EDTA와 같은 chealting agent의 사용의 필요성을 시사한다.

• 한국수정란이식학회지
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• 제19권2호
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• pp.155-163
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• 2004

본 연구는 도축돈의 난소로부터 난자를 채취하여 체외배양시킨 후 세포 안정제와 원심분리 그리고 OPS를 이용한 유리화동결 하였다. 동결 융해한 수정란을 경산돈에 외과적 또는 비외과적으로 이식하여 자돈을 생산하는 것을 목적으로 수행하였다. ${\cdot}$ 도축돈 난소로부터 채란되어진 돼지 미성숙난은 Funahashi 등(1994) 방법에 따라 체외 성숙-수정-배양하였다. 체외배양액은 glucose-free NCSU 23을 이용하였으며, 5일째에 10% Fatal bovine serum (FBS)을 첨가 배반포로 발달을 유도하였다. ${\cdot}$ 체외배양된 수정란은 7.5${\mu}$g.mL cytochalasin B에 30분 처리 후 13,000 rpm에 13분간 원심 분리하였고, ethylene glycol(EG) 동결액으로 처리한 뒤 OPS를 이용하여 동결${\cdot}$융해하였다. ${\cdot}$ 동결수정란과 비동결수정란을 plastic straw에 loading 한 후 3두에는 경산돈에 외과적 방법으로 각각 100개, 100개의 동결수정란과 대조군으로 34개의 비동결수정란을 이식하였고 다른 3두에는 비외과적 방법으로 각각 150개, 150개의 동결수정란과 대조군으로 100개의 비동결수정란을 각각 이식하였다. 외과적이식돈의 대조군에 사용한 신선수정란은 비경산돈 3두에서 채란하였다. ${\cdot}$이식 결과 6두 모두 지연성 발정을 보였으며 이중 동결수정란 이식돈은 임상적으로 정상이였으나 비동결수정란은 이식한 경우는 자궁내막염과 복강탈장이 관찰되었다. 주요 원인은 시술시 기술의 미숙과 야외수술에 의한 감염, 난자의 이동과 수송에 의한 영향 그리고 주입 미숙 등이 주요 원인인 것으로 생각되며 융해 후 생존효율이 높은 수정란의 준비, 이식 기술의 개선과 자궁상태가 청결한 비경산돈의 이식으로 임신율을 높일 수 있을 것으로 기대된다.

• Journal of Periodontal and Implant Science
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• 제29권3호
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• pp.483-509
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• 1999

Biodegradable barrier membrane has been demonstrated to have guided bone regeneration capacity on the animal study. The purpose of this study is to evaluate the effects of cultured calvarial cell inoculated on the biodegradable barrier membrane for the regeneration of the artificial bone defect. In this experiment 35 Sprague-Dawley male rats(mean BW 150gm) were used. 30 rats were divided into 3 groups. In group I, defects were covered periosteum without membrane. In group II, defects were repaired using biodegradable barrier membrane. In group III, the defects were repaired using biodegradable barrier membrane seeded with cultured calvarial cell. Every surgical procedure were performed under the general anesthesia by using with intravenous injection of Pentobarbital sodium(30mg/Kg). After anesthesia, 5 rats were sacrificed by decapitation to obtain the calvaria for bone cell culture. Calvarial cells were cultured with Dulbecco's Modified Essential Medium contained with 10% Fetal Bovine Serum under the conventional conditions. The number of cell inoculated on the membrane were $1{\times}10^6$ Cells/ml. The membrane were inserted on the artificial bone defect after 3 days of culture. A single 3-mm diameter full-thickness artificial calvarial defect was made in each animal by using with bone trephine drill. After the every surgical intervention of animal, all of the animals were sacrificed at 1, 2, 3 weeks after surgery by using of perfusion technique. For obtaining histological section, tissues were fixed in 2.5% Glutaraldehyde (0.1M cacodylate buffer, pH 7.2) and Karnovsky's fixative solution, and decalcified with 0.1M disodium ethylene diaminetetraacetate for 3 weeks. Tissue embeding was performed in paraffin and cut parallel to the surface of calvaria. Section in 7${\mu}m$ thickness of tissue was done and stained with Hematoxylin-Eosin. All the specimens were observed under the light microscopy. The following results were obtained. 1 . During the whole period of experiment, fibrous connective tissue was revealed at 1week after surgery which meant rapid soft tissue recovery. The healing rate of defected area into new bone formation of the test group was observed more rapid tendency than other two groups. 2 . The sequence of healing rate of bone defected area was as follows ； test group, positive control, negative control group. 3 . During the experiment, an osteoclastic cell around preexisted bone was not found. New bone formation was originated from the periphery of the remaing bone wall, and gradually extended into central portion of the bone defect. 4 . The biodegradable barrier membrane was observed favorable biocompatibility during this experimental period without any other noticeable foreign body reaction. And mineralization in the newly formed osteoid tissue revealed relatively more rapid than other group since early stage of the healing process. Conclusively, the cultured bone cell inoculated onto the biodegradable barrier membrane may have an important role of regeneration of artificial bone defects of alveolar bone. This study thus demonstrates a tissue-engineering the approach to the repair of bone defects, which may have clinical applications in clinical fields of the dentistry including periodontics.

• 마케팅과학연구
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• 제19권1호
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• pp.9-21
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• 2009

Recently, the researches to explain Switching Behavior through Theory of Planned Behavior have been discovered. Many researches say that System factors of mobile telecommunication service(MTS) is positively associated with Switching Intention. But There is no difference of system factors between mobile telecommunication services because of IT technique Development. Thus, this study investigated whether switching cost and alternative's attractiveness influence switching intention concerning mobile telecommunication service by adopting Theory of Planned Behavior (TPB) as an underlying theoretical premise. The study also attempted to determine the moderating effects of personal innovativeness on switching intention. For these purposes the author has developed several hypotheses as follows: H-1. The switching cost of different MTS is associated with the attitude of MTS which is using. H-2. The switching cost of different MTS is associated with the subjective norm of MTS which is using. H-3. The switching cost of different MTS is associated with the perceived behavior control of MTS which is using. H-4. The alternative's attractiveness of different MTS is associated with the attitude of MTS which is using. H-5. The alternative's attractiveness of different MTS is associated with the subjective norm of MTS which is using. H-6. The alternative's attractiveness of different MTS is associated with the perceived behavior control of MTS which is using. H-7. The switching cost of different MTS is associated with the switching intention. H-8. The alternative's attractiveness of different MTS is associated with the switching intention. H-9. The attitude of MTS is associated with the switching intention. H-10. The subjective norm of MTS is associated with the switching intention. H-11. The perceived behavior control of MTS is associated with the switching intention. H-12. The personal innovativeness has been a moderating effects to switching intention. Data has been collected from 403 respondents for this study using a questionnaire method. The survey for the actual analysis of the research was done and analyzed with the customers who have an experience of using Mobile telecommunication service and the samples were selected among the middle and high school students who live in Seoul area, the university students who live in Seoul, Gyeonggi and Chungcheng Provinces, and the ordinary workers who are working in Seoul and Gyeonggi Province. The survey was done for 23 days from March 28, 2008 through April 12, 2008. The positive analysis was done with SPSS 12.0K statistics package and visual PLS program using the analysis techniques of frequency analysis, reliability analysis, correlation analysis and factor analysis. In addition, structural equation modeling was conducted using AMOS 5.0. The data was analyzed by frequency analysis using SPSS 12.0 and structural equation modeling using AMOS 5.0. The result of the overall model analysis is as follows: Chi-Square=378.306, d.f.=107, p-value=0.0, GFI=.904, AGFI= 0.863, IFI= 0.939, NFI= 0.917, RMSEA= 0.079, TLI= 0.922. The results of the overall model analysis were coherent. The following study results were revealed: First, switching cost was related positively to attitude, subjective norm and perceived behavior control, three components of TPB. Second, alternative's attractiveness was related negatively to subjective norm but positively to perceived behavior control. Third, switching cost and attitude was related negatively to switching intention, while perceived behavior control was related positively to switching intention. Finally, the study found the moderating effects of personal innovativeness on switching intention. Based on the results, the study offers marketing strategic implications for mobile telecommunication service industry.

• 한국식품영양과학회지
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• 제39권5호
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• pp.750-756
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• 2010

상백피 2.5% 및 강황 0.01%, 상백피 2.5% 및 강황 0.02%의 농도로 각각 혼합한 추출물을 첨가하여 제조한 생면의 품질 특성을 조사하였다. pH 변화는 대조구와 첨가구간의 유의적인 차이는 나타나지 않았으며, 색도의 경우 추출물의 첨가율이 높을수록 명도와 적색도는 감소하였으나 황색도는 증가되었다. 물성은 첨가량이 증가할수록 경도와 검성은 증가하였으며, 탄력성과 응집성은 유의적인 차이를 보이지 않았다. 저장성을 알아본 결과 저장 21일차에서 상백피 2.5%와 강황 0.02% 추출 혼합물 첨가구가 대조구에 비해 생균수가 약 2 log cycle 정도 감소되어 저장 기간을 연장할 수 있을 것으로 사료된다. 산화도 역시 첨가구가 대조구에 비해 산화도가 감소하여 생면의 산화방지에 도움을 줄 것으로 보인다. 기호도에 있어 전체적인 호감도에서 상백피 2.5%와 강황 0.02% 추출 혼합물 첨가구가 대조구에 비해 높은 점수를 받아 이상의 결과를 종합해 볼 때 상백피 2.5% 및 강황 0.02%의 농도로 첨가할 경우 생면의 저장성, 품질증진 및 관능개선에 효과가 있을 것으로 사료된다.

• Applied Microscopy
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• 제30권4호
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• pp.347-355
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• 2000

Zinc는 인체 내에 철$(Fe^{2+})$다음으로 많은 trace element로서 200여개 효소의 기능에 필수적일 뿐만 아니라 신경계통내에서는 신경조절물질로 작용한다. 뿐만 아니라 허혈, 간질 및 퇴행성 뇌질환의 주요 병리기전에도 관여되어 있다. 그러나 대부분의 Zinc는 단백질에 결합되어 (bound form)신경세포의 세포질 및 핵질내에 존재하고, 10% 이하의 Zinc는 이온상태(free form, $Zn^{2+}$)로 신경종말 (Zinc enriched terminal)에 있는데 , 후자만이 조직화학법으로 가시화된다. 최근까지 새로 개발된 조직화학법으로 Zinc enriched(ZEN)neurons의 분포에 관한 연구가 각광받고 있으나, 국내에서는 이에 대한 연구가 전무한 실정이다. 이에 본 연구자는 고전적인 조직화학법의 기본 원리를 소개하고, 렛드 중추신경계통내 Zinc의 분포를 광학 및 전자현미경으로 관찰하고자 하였다. 본 연구에서 사용된 실험동물은 Wistar 계통의 랫드(10주령)와 BALB/c 마우스이며, 마취제로는 Pentobarbital(50mg/kg)을 이용하였다. 생체 뇌조직내 이온상태의 $Zinc(Zn^{2+})$를 침전시키기 위하여 selenium(10mg/kg, i.p.)을 처리하였고, 1시간 후 3% Glutaraldehyde액으로 관류고정하여 동물을 희생시켰다. 뇌와 척수를 꺼내어 sucrose에 가라앉을때 까지 담가두었다가 Dry Ice를 이용하여 얼리고, Freezing microtome위에서 $30{\mu}m$두께의 절편을 작성하였다. 조직절편내 $Zn^{2+}$을 동정하기 위한 조직화학법으로는 autometallography (AMG) (Danscher, 1985)를 이용하였다. 광학현미경하에서 밝혀진 Zinc의 분포는 해마복합체를 비롯한 종뇌의 여러부위에 고농도로 분포하였고, 척수에는 중간정도, 그리고 소뇌 및 뇌간에는 매우 낮은 농도로 분포하였다. 전자현미경에서 관찰된 AMG염색과립(silver grains)은 신경종말에 있는 연접소포에 국한되었으며, 이러한 ZEN terminals은 주위 여러 신경세포의 돌기(dendrites)및 세포체 (soma)에 특이한 연접을 이루고 있었다. 즉 후각망울을 포함한 종뇌에서는 주로 비대칭연접 (asymmetrical synapses)이 관찰되었던 반면에, 척수에서는 대칭연접(symmetrical synapses)을 이루고 있었다. 이상의 결과를 종합하면, 신경종말내 연접소포에 Zinc를 함유하고 있는 소위 ZEN terminals은 중추신경계통에 광범위하게 분포하고 있으며 또한 신경부위에 따라 다양한 분포와 미세구조의 차이를 보였다. 이러한 사실은 중추신경계통내에서 Zinc가 영위하는 신경생물학적 기능이 신경부위에 따라 다양할 것임을 시사한다.