• The Plant Pathology Journal
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• 제35권6호
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• pp.575-584
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• 2019

Colletotrichum acutatum is a species complex responsible for anthracnose disease in a wide range of host plants. Strain C. acutatum KC05, which was previously isolated from an infected pepper in Gangwon Province of South Korea, was reidentified as C. scovillei using combined sequence analyses of multiple genes. As a prerequisite for understanding the pathogenic development of the pepper anthracnose pathogen, we optimized the transformation system of C. scovillei KC05. Protoplast generation from young hyphae of KC05 was optimal in an enzymatic digestion using a combined treatment of 2% lysing enzyme and 0.8% driselase in 1 M NH₄Cl for 3 h incubation. Prolonged incubation for more than 3 h decreased protoplast yields. Protoplast growth of KC05 was completely inhibited for 4 days on regeneration media containing 200 ㎍/ml hygromycin B, indicating the viability of this antibiotic as a selection marker. To evaluate transformation efficiency, we tested polyethylene glycol-mediated protoplast transformation of KC05 using 19 different loci found throughout 10 (of 27) scaffolds, covering approximately 84.1% of the entire genome. PCR screening showed that the average transformation efficiency was about 17.1% per 100 colonies. Southern blot analyses revealed that at least one transformant per locus had single copy integration of PCR-screened positive transformants. Our results provide valuable information for a functional genomics approach to the pepper anthracnose pathogen C. scovillei.

• 한국세라믹학회지
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• 제43권6호
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• pp.362-368
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• 2006

Epitaxial $Ba_{0.5}Sr_{0.5}TiO_3$ (BSTO) thin films have been grown on TiN buffered Si (001) substrates by Pulsed Laser Deposition (PLD) method and the effects of substrate temperature and oxygen partial pressure during the deposition on their dielectric properties and crystallinity were investigated. The crystal orientation, epitaxy nature, and microstructure of oxide thin films were investigated using X-Ray Diffraction (XRD) and Transmission Electron Microscopy (TEM). Thin films were prepared with laser fluence of $4.2\;J/cm^2\;and\;3\;J/cm^2$, repetition rate of 8 Hz and 10 Hz, substrate temperatures of $700^{\circ}C$ and ranging from $350^{\circ}C\;to\;700^{\circ}C$ for TiN and oxide respectively. BSTO thin-films were grown on TiN-buffered Si substrates at various oxygen partial pressure ranging from $1{\times}10^{-4}$ torr to $1{\times}10^{-5}$ torr. The TiN buffer layer and BSTO thin films were grown with cube-on-cube epitaxial orientation relationship of $[110](001)_{BSTO}{\parallel}[110](001)_{TiN}{\parallel}[110](001)_{Si}$. The crystallinity of BSTO thin films was improved with increasing substrate temperature. C-axis lattice parameters of BSTO thin films, calculated from XRD ${\theta}-2{\theta}$ scans, decreased from 0.408 m to 0.404 nm and the dielectric constants of BSTO epitaxial thin films increased from 440 to 938 with increasing processing oxygen partial pressure.

• 한국주거학회논문집
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• 제20권4호
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• pp.39-47
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• 2009

The purpose of this study is to find out the changes on the metropolitan condominium apartment unit plans based on the interior spaces related to women such An-bang (master bedroom), bathroom and attached spaces to the master bedroom, living room, diningroom, kitchen, utility room, and balcony. This study used the content analysis method and space syntax. Contents were limited to the floor plans with 3 rooms type and about 105 m2 in the Korean apartment encyclopedia. The unit plans of 2,278, built from October 1975 to January 2008, were analyzed and basically SPSS package 16.0 was employed. The results were as followed. 1) Living room was a core space and an increasing tendency. After 2001, the design trend of living room connected with kitchen and dining room, and dining room changed into one space combining with women's working space such kitchen. It meaned women's working activities became into family public activities as to depending open access. 2) Kitchen had a characteristics that was connected to utility room or back balcony, and the number of extra kitchen increased. Also, the size of DK decreased, but utility size little increased. It was showed the characteristic of this space was still for women. 3) An-bang had a little strong characteristics of married coulpe or women's personal space with bathroom or dress room/powder room. 4) In addition, the values of integration and control of living room and DK were highest. It meant these space were centered in unit plans. Next, the An-bang and dress room/powder room were high. Utility room or balcony's were next, and these were women's working space. Therefore, family's public space was most important, and married couple or women's personal and working space were later in planning.

• Structural Engineering and Mechanics
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• 제35권5호
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• pp.571-592
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• 2010

An energy-based fatigue life prediction framework was previously developed by the authors for prediction of axial, bending and shear fatigue life at various stress ratios. The framework for the prediction of fatigue life via energy analysis was based on a new constitutive law, which states the following: the amount of energy required to fracture a material is constant. In the first part of this study, energy expressions that construct the constitutive law are equated in the form of total strain energy and the distortion energy dissipated in a fatigue cycle. The resulting equation is further evaluated to acquire the equivalent stress per cycle using energy based methodologies. The equivalent stress expressions are developed both for biaxial and multiaxial fatigue loads and are used to predict the number of cycles to failure based on previously developed prediction criterion. The equivalent stress expressions developed in this study are further used in a new finite element procedure to predict the fatigue life for two and three dimensional structures. In the second part of this study, a new Quadrilateral fatigue finite element is developed through integration of constitutive law into minimum potential energy formulation. This new QUAD-4 element is capable of simulating biaxial fatigue problems. The final output of this finite element analysis both using equivalent stress approach and using the new QUAD-4 fatigue element, is in the form of number of cycles to failure for each element on a scale in ascending or descending order. Therefore, the new finite element framework can provide the number of cycles to failure at each location in gas turbine engine structural components. In order to obtain experimental data for comparison, an Al6061-T6 plate is tested using a previously developed vibration based testing framework. The finite element analysis is performed for Al6061-T6 aluminum and the results are compared with experimental results.

• International journal of advanced smart convergence
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• 제7권4호
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• pp.50-56
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• 2018

The Internet of Things (IoT) and Visible Light Communication (VLC) is opening up new services in lighting industry by integrating sensory network features in addition to standard illumination functionality. In this progressive developments, the next generation lighting devices for smart homes are capable to sense the environmental conditions and transfer the captured data through lights to gateway controller to access remotely. The smart home environmental sensor information's are few kbps only so VLC systems need to built-in with low rate light connectivity to transfer data to the gateway. To provide error free communication, the quality of a received light signal is important to be considered when designing an VLC receiver. Therefore, this paper proposes the design of robust low rate IoL receiver design using NCP302 voltage detector for micro controller to adapt the IoT/IoL front end module for system integration. To evaluate the proposed system performance, the Arduino UNO based IoT/IoL controller designed with lighting, sensors and lights connectivity interfaces. The experimental result shows that the robust interference rejection is feasible on proposed VOL receiver and possible to have an error-free communication up to 10 kbps at a low SNR using OOK modulation.

• Advances in aircraft and spacecraft science
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• 제8권4호
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• pp.345-372
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• 2021

The analysis of nonlinear vibrations, buckling, post-buckling, flutter boundary determination and post-flutter behavior of a homogeneous curved plate assuming cylindrical bending is conducted in this article. Other assumptions include simply-supported boundary conditions, supersonic aerodynamic flow at the top of the plate, constant pressure conditions below the plate, non-viscous flow model (using first- and third-order piston theory), nonlinear structural model with large deformations, and application of mechanical and thermal loads on the curved plate. The analysis is performed with constant environmental indicators (flow density, heat, Reynolds number and Mach number). The material properties (i.e., coefficient of thermal expansion and modulus of elasticity) are temperature-dependent. The equations are derived using the principle of virtual displacement. Furthermore, based on the definitions of virtual work, the potential and kinetic energy of the final relations in the integral form, and the governing nonlinear differential equations are obtained after fractional integration. This problem is solved using two approaches. The frequency analysis and flutter are studied in the first approach by transferring the handle of ordinary differential equations to the state space, calculating the system Jacobin matrix and analyzing the eigenvalue to determine the instability conditions. The second approach discusses the nonlinear frequency analysis and nonlinear flutter using the semi-analytical solution of governing differential equations based on the weighted residual method. The partial differential equations are converted to ordinary differential equations, after which they are solved based on the Runge-Kutta fourth- and fifth-order methods. The comparison between the results of frequency and flutter analysis of curved plate is linearly and nonlinearly performed for the first time. The results show that the plate curvature has a profound impact on the instability boundary of the plate under supersonic aerodynamic loading. The flutter boundary decreases with growing thermal load and increases with growing curvature.

• 한국산업융합학회 논문집
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• 제26권4_2호
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• pp.697-703
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• 2023

Intertidal zones, akin to tidal flats, are among the potential areas where underwater cultural heritage might be submerged. However, the shallow depths in these regions present challenges for conventional vessel-based survey methods. Moreover, during low tides, intertidal zones transform into tidal flats, limiting the efficiency of survey efforts due to restricted access and potential risks. As a result, proper underwater cultural heritage surveys encounter difficulties in these environments. In recent times, extensive research is underway to address these issues by investigating underwater cultural heritage surveys in intertidal zones, encompassing diverse fields, including equipment-based investigations. This study aimed to explore the feasibility of utilizing unmanned aerial vehicles (UAVs) to conduct intertidal cultural heritage surveys, employing aerial photography and 3D mapping to create detailed orthoimages and 3D models. The study focused on assessing the potential application of these techniques for cultural heritage surveying within intertidal zones. Notably, the survey conducted in Jindo's Naesan-ri demonstrated high-resolution capabilities, enabling the distinction of actual pottery fragments mixed within gravel fields. Similarly, in the survey of Jindo's Byeokpa-hang, it was found that a wooden pillar structure existed in a section about 200m long. The integration of various sensors, including LiDAR, with UAVs allows for diverse investigation possibilities, including bathymetric measurements, and is expected to facilitate the acquisition of varied datasets for further research and assessment.

• 한국환경농학회지
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• 제19권4호
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• pp.339-344
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• 2000

축산폐수를 처리하는 연못시스템의 1차연못 처리수(평균 $BOD_5\;49.1mg{\cdot}L^{-1}$, $T-N\;48.6\;mg{\cdot}L^{-1}$)를 1주에 2 - 3회 실험양어지에 공급하여 잉어의 성장을 조사하였다. 처리수 공급유량과 실험양어지의 유량이 1:4가 유지되도록 공급하였으며, 실험양어지의 평균 $BOD_5$는 $15\;-\;20\;mg{\cdot}L^{-1}$였다. 양어지에 수용한 잉어의 평균 체중은 8월에 204.2 g에서 9월과 10월에 210.4 g와 219.3 g로 각각 늘었으며 60일간 사육에서 생잔율이 82%였다. 운반 및 조사시 잉어에 주는 스트레스를 감안하면 양어지의 수질과 환경은 잉어 성장에 나쁘지 않았다고 여겨진다. 8월부터 10월 사이의 다소 낮은 성장률은 양어지의 물을 빼내고 잉어를 잡아 체중과 체장을 측정하는 과정이 잉어에 스트레스를 준데 원인이 있다고 사료된다. 월동 후인 이듬해 4월에 잉어의 평균 체중은 205.3g로 감소하였으며 전년도 10월과 비교하여 생잔율은 83%였다. 일반적으로 잉어는 월동 후에 체중이 약 10 - 20% 감소하는 경향이 있다. 양어지 실험결과 양어지의 평균 $BOD_5$가 $15\;-\;20\;mg{\cdot}L^{-1}$로 유지되면 용존산소결핍으로 잉어가 일시에 죽는 현상은 일어나지 않음을 알 수 있었다. 평균 $BOD_5$와 T-N이 각각 27.9, $30.8\;mg{\cdot}L^{-1}$인 2차연못에 체중 약 100g 정도의 잉어 10마리를 8월에 넣어 보았으나 1주일 이내에 모두 죽었다. 새벽 무렵의 급격한 용존산소결핍 현상에 원인이 있다고 사료된다. 연못시스템의 3차연못에 잉어를 직접 털어 성장을 분석하였다. 3차연못의 평균 $BOD_5$와 T-N은 각각 $19.8\;mg{\cdot}L^{-1}$, $21.0\;mg{\cdot}L^{-1}$였다. 3차연못은 2차연못에서 발생한 조류(algae)가 죽어 연못바닥으로 침전되는 마무리연못(maturation pond)으로 연못전체가 호기성을 유지하고 있었다. 3차연못에 8월에 넣은 잉어의 평균체중은 107.5g였으며 2개월 후에 165.2g으로 증가하였다. 사육 1년 후의 평균체중은 478.2g으로 약 3배 증가했으며, 1년 후의 잉어 생잔율도 86%로 높게 나타났다. 월동을 했다는 것을 고려한다면 매우 좋은 성장이다. 생잔율이 높게 나타난 원인은 측정 횟수가 적어 측정에 따른 스트레스가 적었다는 점과 3차연못의 환경조건 (수질, 먹이 등)이 잉어사육에 적합했다고 사료된다. 사료공급 없이 하${\cdot}$폐수를 재활용하는 세계 여러나라의 잉어 생산량은 약 $18\;-\;l37g{\cdot}m^{-2}year^{-1}$이다. 3차연못 잉어생산량은 $125\;g{\cdot}m^{-2}year^{-1}$로 아주 양호한 생산량이다. 산업폐수가 유입되지 않은 생활하수나 축산폐수의 처리수를 이용한 양어의 고기는 생체실험결과 인체에 해가 없다고 규명되어 왔다.$^{15,16),17)$ 축산폐수를 자연생태적으로 처리하는 연못시스템의 연못에서 잉어를 사육할 경우 연못의 수질이 2차처리수준인 $BOD_5\;20\;mg{\cdot}L^{-1}$ 정도를 유지하는 것이 적절하다고 사료된다. 연못시스템은 축산지수를 효율적으로 처리할 수 있을 뿐만 아니라, 양어의 수확으로 축산농가의 소득증대에도 기여할 수 있다.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1991년도 한국자동제어학술회의논문집(국제학술편); KOEX, Seoul; 22-24 Oct. 1991
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• pp.1649-1654
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• 1991

In 1995 the VSOP satellite, which is called MUSES-B in Japan, will be launched under the VLBI Space Observatory Programme(VSOP) promoted by ISAS(Institute of Space and Astronautical Science) of Japan. We are now developing the GPS Receiver(GPSR) and On-board Orbit Determination System. This paper describes the GPS(Global Positioning System), VSOP, GPSR(GPS Receiver system) configuration and the results of the GPS system analysis. The GPSR consists of three GPS antennas and 5 channel receiver package. In the receiver package, there are two 16 bits microprocessing units. The power consumption is 25 Watts in average and the weight is 8.5 kg. Three GPS antennas on board enable GPSR to receive GPS signals from any NAVSTARs(GPS satellites) which are visible. NAVSATR's visibility is described as follows. The VSOP satellite flies from 1, 000 km to 20, 000 km in height on the elliptical orbit around the earth. On the other hand, the orbit of NAVSTARs are nearly circular and about 20, 000 km in height. GPSR can't receive the GPS signals near the apogee, because NAVSTARs transmit the GPS signals through the NAVSTAR's narrow beam antennas directed toward the earth. However near the perigee, GPSR can receive from 12 to 15 GPS signals. More than 4 GPS signals can be received for 40 minutes, which are related to GDOP(Geometric Dillusion Of Precision of selected NAVSTARs). Because there are a lot of visible NAVSTARs, GDOP is small near the perigee. This is a favorqble condition for GPSR. Orbit determination system onboard VSOP satellite consists of a Kalman filter and a precise orbit propagator. Near the perigee, the Kalman filter can eliminate the orbit propagation error using the observed data by GPSR. Except a perigee, precise onboard orbit propagator propagates the orbit, taking into account accelerations such as gravities of the earth, the sun, the moon, and other acceleration caused by the solar pressure. But there remain some amount of calculation and integration errors. When VSOP satellite returns to the perigee, the Kalman filter eliminates the error of the orbit determined by the propagator. After the error is eliminated, VSOP satellite flies out towards an apogee again. The analysis of the orbit determination is performed by the covariance analysis method. Number of the states of the onboard filter is 8. As for a true model, we assume that it is based on the actual error dynamics that include the Selective Availability of GPS called 'SA', having 17 states. Analytical results for position and velocity are tabulated and illustrated, in the sequel. These show that the position and the velocity error are about 40 m and 0.008 m/sec at the perigee, and are about 110 m and 0.012 m/sec at the apogee, respectively.

• 한국수산과학회지
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• 제31권2호
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• pp.202-209
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• 1998

본 연구에서는 부유식 해상관측시설의 파랑중 운동응답 및 표류력 해석을 위해 Green 적분방정식에 기초한 3차원 수치해법을 개발하였다. 본 방법에서는 소오스분포와 더브렛분포를 함께 사용하였으며, 판요소로는 3각형 요소와 4각형 요소를 병행 사용하였다. 불규칙파수 현상을 제거하기 위해 개량된 적분방정식 해법을 적용하였으며, 시간평균 표류력의 계산은 원인별 성분파악이 가능한 물체표면 직접적분법을 사용하였다. 개발된 전산 프로그램의 검증을 위해 비교자료가 있는 구형 부유체에 대한 계산이 수행되었고, 이에 대한 계산을 통해 개발된 프로그램으로부터 신뢰성있는 결과를 얻을 수 있음을 확인하였다. 실제시설에 대한 적용예로서 직경 2.6m 흘수 3.77m인 원통형 해상관측용 부이에 대한 계산을 수행하여 그 운동특성 및 표류력 특성을 고찰하였다. 운동응답 해석결과는 공진주파수를 설치해역의 파랑 탁월주파수 범위밖에 놓이도록 부이의 형상과 치수를 조정하는데 활용할 수 있고, 또 이들 계산을 통해 댐퍼 등의 설치효과도 미리 예측할 수 있다. 또한, 계산된 표류력은 황천중에서 계류계에 걸리는 최대하중을 예측하는데 이용할 수 있으므로 계류계의 설계에 있어 중요한 기초자료로 활용된다. 본 수치해법은 원칙적으로 대상 부유체의 형상에 제약을 받지 않으므로 향후 다양한 형상의 부유식 해상관측 시설들의 설계 및 설치$\cdot$운용에 폭 넓게 적용할 수 있다.