• Journal of Microbiology and Biotechnology
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• 제14권2호
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• pp.297-301
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• 2004

The dTDP-D-glucose 4,6-dehydratase from Salmonella enterica was immobilized using covalent binding to cyanogen bromide activated sepharose. The immobilized enzyme was used to produce dTDP-4-keto-6-deoxy-D-glucose, a key sugar intermediate that can be used economically to produce diverse classes of unusual sugars appended in various antibiotics. The enzyme was immobilized on the sepharose after activation with cyanogen bromide. The maximum immobilization (80.03％) was achieved after 14 h of coupling. The covalently immobilized enzyme was stable, and an average of 78.4 ％ conversion was achieved until 120 h of immobilization when it was repeatedly used. Similar conversion was noticed for the first batch using the enzyme entrapped-hydrogel but activity was gradually decreased in the following batches. The production of dTDP-4-keto-6-deoxy-D-glucose by using an immobilized enzyme has high potential for commercial application.

• 로봇학회논문지
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• 제4권4호
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• pp.298-304
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• 2009

This paper describes an algorithm that improves 3D reconstruction result using a multi-sensor fusion disparity map. We can project LRF (Laser Range Finder) 3D points onto image pixel coordinatesusing extrinsic calibration matrixes of a camera-LRF (${\Phi}$, ${\Delta}$) and a camera calibration matrix (K). The LRF disparity map can be generated by interpolating projected LRF points. In the stereo reconstruction, we can compensate invalid points caused by repeated pattern and textureless region using the LRF disparity map. The result disparity map of compensation process is the multi-sensor fusion disparity map. We can refine the multi-sensor 3D reconstruction based on stereo vision and LRF using the multi-sensor fusion disparity map. The refinement algorithm of multi-sensor based 3D reconstruction is specified in four subsections dealing with virtual LRF stereo image generation, LRF disparity map generation, multi-sensor fusion disparity map generation, and 3D reconstruction process. It has been tested by synchronized stereo image pair and LRF 3D scan data.

• 한국당과학회:학술대회논문집
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• 한국당과학회 2006년도 제1회 연례학술대회
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• pp.52-52
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• 2006

• 한국정보통신학회논문지
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• 제20권12호
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• pp.2363-2369
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• 2016

본 논문에서는 집적 영상과 컴퓨터 재생 기술을 활용한 3차원 QR 코드 생성 기술을 제안한다. 제안하는 기술에서는 2차원 QR 코드를 네 개의 면으로 나누어 각각의 면을 서로 다른 깊이에 배치한 뒤 깊이가 추가된 3차원 QR 코드를 합성조리개 집적 영상과 컴퓨터 재생 기술을 사용하여 생성한다. 이 과정에서 3차원 영상 기술 중 하나인 집적 영상 기술을 사용한다. 마지막으로, 3차원 QR 코드를 집적영상을 이용하여 컴퓨터 재생을 한 뒤 각 면을 배치했던 깊이 부분에서 영상을 재생하고 조합하면 3차원 QR 코드를 읽을 수 있다. 따라서 QR 코드를 읽을 때 보안을 강화할 수 있다. 본 논문에서는 제안된 기술이 QR 코드를 읽을 때 보안을 강화할 수 있음을 보이기 위하여 광학적 실험과 컴퓨터 재생을 수행하였다. 또한, 재생 깊이를 알고 있을 때, 3차원 QR 코드를 읽을 수 있음을 보였다.

• BMB Reports
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• 제37권4호
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• pp.503-506
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• 2004

Glucose-1-phosphate uridylyltransferase from E. coli K12 was used to convert uridine-5'-triphosphate and glucose-1-phosphate to UDP-D-glucose. The conversion was efficient and completed within 5 minutes under the employed conditions. In addition, thymidine-5'-monophosphate kinase and acetate kinase were proven to be non-specific, converting udridine-5'-monophosphate to uridine-5'-triphosphate with 55% conversion after 6 h, which was much slower than the production of TTP under the same conditions (complete conversion within one hour). Since these two reactions could proceed under the same conditions, a one-pot synthesis of UDP-D-glucose with ATP regeneration was designed from easily available starting materials, and conversion up to 40% by HPLC peak integration was achieved given a reaction time of 4 h.

• Molecules and Cells
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• 제37권2호
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• pp.172-177
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• 2014

A glycosyltransferase, YjiC, from Bacillus licheniformis has been used for the modification of the commercially available isoflavonoids genistein, daidzein, biochanin A and formononetin. The in vitro glycosylation reaction, using UDP-${\alpha}$-D-glucose as a donor for the glucose moiety and aforementioned four acceptor molecules, showed the prominent glycosylation at 4' and 7 hydroxyl groups, but not at the $5^{th}$ hydroxyl group of the A-ring, resulting in the production of genistein 4'-O-${\beta}$-D-glucoside, genistein 7-O-${\beta}$-D-glucoside (genistin), genistein 4',7-O-${\beta}$-D-diglucoside, biochanin A-7-O-${\beta}$-D-glucoside (sissotrin), daidzein 4'-O-${\beta}$-D-glucoside, daidzein 7-O-${\beta}$-D-glucoside (daidzin), daidzein 4', 7-O-${\beta}$-D-diglucoside, and formononetin 7-O-${\beta}$-D-glucoside (ononin). The structures of all the products were elucidated using high performance liquid chromatography-photo diode array and high resolution quadrupole time-of-flight electrospray ionization mass spectrometry (HR QTOF-ESI/MS) analysis, and were compared with commercially available standard compounds. Significantly higher bioconversion rates of all four isoflavonoids was observed in both in vitro as well as in vivo bioconversion reactions. The in vivo fermentation of the isoflavonoids by applying engineered E. coli $BL21(DE3)/{\Delta}pgi{\Delta}zwf{\Delta}ushA$ overexpressing phosphoglucomutase (pgm) and glucose 1-phosphate uridyltransferase (galU), along with YjiC, found more than 60% average conversion of $200{\mu}M$ of supplemented isoflavonoids, without any additional UDP-${\alpha}$-D-glucose added in fermentation medium, which could be very beneficial to large scale industrial production of isoflavonoid glucosides.

• Journal of International Society for Simulation Surgery
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• 제4권1호
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• pp.13-16
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• 2017

Fibular free flap reconstruction is the flap of the choice in long-span mandibular bone reconstruction. The most common disadvantage of the fibular flap is short bone height to install dental implant. Double barrel fibular flap has been tried, however, bulky flap in the oral cavity hinder its use. Titanium reconstruction plate has been used simultaneously with the free fibular flap to stabilize occlusion and to fix the fibular flap. In this study, titanium reconstruction plate was fixed in the lower border of the mandible and the fibular free flap was fixed in the superior border of the titanium plate to improve implant-crown ratio. This new technique improved the longevity of the dental prosthodontics with dental implants.

• ETRI Journal
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• 제28권4호
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• pp.521-524
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• 2006

In this letter, we propose a novel computational integral imaging reconstruction technique based on a lenslet array model. The proposed technique provides improvement of viewing images by extracting multiple pixels from elemental images according to ray tracing based on the lenslet array model. To show the feasibility of the proposed technique, we analyze it according to ray optics and present the experimental results.

• Journal of Biosystems Engineering
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• 제42권4호
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• pp.339-349
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• 2017

Purpose: This review focuses on the major 3-D image reconstruction techniques and their applications in plant and animal morphological analysis. Methods & Results: This paper begins with an overview of major 3-D image reconstruction techniques and their basic principles. Subsequently, their applications in plant and animal morphological analysis are reviewed. A discussion on the limitations and future research direction of 3-D imaging techniques for accurate, fast measurements and modeling of plant and animal morphological analysis follows. Conclusions: Owing to the increasing demand for plant and animal morphological analysis, the application of 3-D imaging techniques will increase in popularity among researchers and the agricultural industry.

• Investigative Magnetic Resonance Imaging
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• 제11권1호
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• pp.33-38
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• 2007

목적: 반얼굴 연축 환자에서 새로운 삼차원 중첩 자기공명 혈관 조영술 기법(3-D overlapped reconstruction MR angiographic technique, 3-D ORMRA) 을 기존의 MRA 원천영상과 비교하여 그 유용성을 알아보고자 하였다. 대상 및 방법: 수술로 반얼굴 연축이 증명된 총 27명을 대상으로 하였다. 모든 환자에서 전향적으로 기존의 MRA 원천영상과 3-D fast imaging employing steady state acquisition (FIESTA) 영상을 얻었다. 이 후 작업대 (workstation)에서 3-D MRA 영상을 만들고 GE A/W 4.2 add/sub software를 이용하여 이를 FIESTA영상과 겹쳐 3-D ORMRA영상을 얻었다. 그리고 나서 기존의 MRA 원천영상과 3-D ORMRA영상에서 각 각 얼굴신경의 신경근출구부와 병적 압박혈관 사이의 관계를 분석하였다. 결과: 기존의 MRA원천영상에서는 27명중 25명의 환자에서 얼굴 신경근출구부에서의 병적 압박혈관을 구별 할 수 있었고, 3-D ORMRA영상에서는 모든 환자에서 병적 압박혈관을 구별 할 수 있었으며, 이는 수술소견과 일치하였다. 무엇보다 3-D ORMRA영상에서 얼굴 신경근출구부와 병적 압박혈관 사이의 공간적인 관계를 좀 더 분명하게 볼 수 있었다. 결론: 3-D ORMRA 기법은 기존의 MRA 영상기법과 비교하여, 반얼굴 연축 환자에서 매우 유용하고 더 정확한 정보를 주는 방법이다.