• 한국콘텐츠학회논문지
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• 제20권7호
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• pp.274-281
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• 2020

본 연구는 4-gram 연어분석으로 남북한 고등학교 영어교과서를 비교분석하고자 하는 것이 목적이다. N-gram 분석은 그동안 우리가 알고 있는 관습적인 관용어와는 달리 코퍼스를 구성하여 기계적인 방법으로 물리적으로 함께 공기하는 빈도가 높은 낱말군을 객관적인 방법으로 추출하여 분석하는 것이다. 본 연구의 목적은 AntConc의 N-gram 분석 도구로 4-gram 연어를 남북한 영어교과서 코퍼스에서 찾아서 비교 분석해 보는 것이다. 분석의 대상은 북한의 2013 교육개혁에 따른 북한 고등중학교 영어교과서와 남한의 2015교육과정에 따른 고등학교 영어교과서로 구성된 코퍼스에서 구어와 문어의 token과 type을 구분하여 분석 비교한다. 이를 분석대상으로 하여 코퍼스의 4-gram 연어를 문법범주와 기능범주로 나눈 준거를 통해서 분석하였다. 문법범주는 크게 명사구, 동사구, 전치사구, 부분절 그리고 기타로 나누어 범주화하고 기능범주는 지칭, 텍스트의 조직, 입장과 기타로 나누었다. 분석한 결과 4-gram 연어에 나타난 구어와 문어 모두 남한의 영어교과서가 북한의 영어교과서 보다 token과 type의 수가 상대적으로 많았다. 그리고 문법범주에는 남북한 모두 영어교과서에 동사구와 부분절 형태의 4-gram 연어가 가장 많았으며 기능범주에는 남북한 모두 영어교과서에 입장 기능과 관련된 4-gram 연어가 가장 많았다.

• 미생물학회지
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• 제9권2호
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• pp.47-54
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• 1971

This study is concerned to the microbial population and its effects in the fish paste products. Experimental results were summarized as following orders ; 1) The number of bacteria in accordance with raw materials and coocking site waas known to as ; Wheat flor - $7.0{\times}10^8$/gram , Brayed fish meat - $2.0{\times}10^7$/gram, Cooking table - $6.1{\times}10^6$/$1.54cm^2$Chopped fish meat -$4.1{\times}10^6$/gram, Wooden plate - $5.5{\times}10^5$/218.32cm, Sodium chloride - $8.8{\times}10^4$/gram, Wheat starch - $4.5{\times}10^4$/gram, Fish meat - $2.3{\times}10^4$/gram. However, there were a few bacterial numbers less than 300 per gram of raw materials, such as monosodium glutamate, tap water, sugar, saccharine and eggs. 2) Bacterial population in the raw materials might be changed according to the conditions of preservation. 3) When the heat tratment is used for the preservation, the thermal effects were rarely found at the interior part of fish paste products. Conduction of heat into the interior part dose not rise about $84^{\circ}C$ and the survival of bacteria could be possible under the condition. Therefore, cooking being used requires further studies. 4) Number of bacteria in the fish paste products sold in the market was higher on April and May than those on June. Especially on June, the number of bacteriz in the interior part were found higher than those in the exterior part of the products.

• 대한수학회보
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• 제41권4호
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• pp.739-777
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• 2004

An analogue of the Gram's inequality for n-inner product spaces is given. Further, a number of inequalities involving Gram's determinant are stated and proved in terms of n-inner products.

• 미생물학회지
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• 제47권1호
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• pp.50-55
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• 2011

폐수 처리 시스템의 그람 양성 세균을 간편하고 신속하게 모니터링할 수 있는 그람 염색 방법을 개발하였다. 각각 4종류의 그람 양성 세균과 음성 세균의 배양액을 polyethersulfone membrane filter와 Toluidine Blue-O로 여과 및 염색하였다. 염색된 여과막을 건조시키고 스캔하였으며, 영상 분석 프로그램을 이용하여 빨강색, 녹색, 파랑색의 강도를 측정하는 정량적 색상 이미지 분석법을 개발하였다. 그람 양성 세균의 경우 그람 음성 세균보다 빨간색과 초록색 수치가 높게 나타났다. 본 방법을 그람 양성 세균이 혼합된 활성슬러지에 적용한 결과, 활성슬러지의 경우 플록의 불규칙한 크기와 형태로 측정이 어렵다 할지라도 플록 분산 기술을 이용하여 활성슬러지의 그람 양성 세균의 변화를 모니터링 할 수 있었다. 활성슬러지에서도 그람 양성 세균의 비율이 높아질수록 빨간색과 초록색 수치가 증가하였다. 본 연구에서 개발된 방법은 폐수처리 시스템에 존재하는 그람 양성 세균을 신속하고 정량적으로 모니터링 할 수 있다.

• The Korean Journal of Physiology and Pharmacology
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• 제13권1호
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• pp.39-47
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• 2009

Gaegurin 4(GGN 4), an antimicrobial peptide isolated from a Korean frog, is five times more potent against Gram-positive than Gram-negative bacteria, but has little hemolytic activity. To understand the mechanism of such cell selectivity, we examined GGN4-induced $K^+$ efflux from target cells, and membrane conductances in planar lipid bilayers. The $K^+$ efflux from Gram-positive M. luteus(2.5 ${\mu}g/ml$) was faster and larger than that from Gram-negative E. coli(75 ${\mu}g/ml$), while that from RBC was negligible even at higher concentration(100 ${\mu}g/ml$). GGN4 induced larger conductances in the planar bilayers which were formed with lipids extracted from Gram-positive B. subtilis than in those from E. coli(p<0.01), however, the effects of GGN4 were not selective in the bilayers formed with lipids from E. coli and red blood cells. Addition of an acidic phospholipid, phosphatidylserine to planar bilayers increased the GGN4-induced membrane conductance(p<0.05), but addition of phosphatidylcholine or cholesterol reduced it(p<0.05). Transmission electron microscopy revealed that GGN4 induced pore-like damages in M. luteus and dis-layering damages on the outer wall of E. coli. Taken together, the present results indicate that the selectivity of GGN4 toward Gram-positive over Gram-negative bacteria is due to negative surface charges, and interaction of GGN4 with outer walls. The selectivity toward bacteria over RBC is due to the presence of phosphatidylcholine and cholesterol, and the trans-bilayer lipid asymmetry in RBC. The results suggest that design of selective antimicrobial peptides should be based on the composition and topology of membrane lipids in the target cells.

• 대한간호학회지
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• 제9권2호
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• pp.17-26
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• 1979

This research is to prevent the infection of maternity in the hospital by examining the microbes contaminations in maternity through airbone microbes and those who are engaged in the ward of O.B. ＆ G.Y. and to furnish the basic data available to hospital management. The bacterial growth of airbone microbes contaminations in nosocomial air and who thor the nasal cavity of passers by (doctors, nurses, parturient women) who went to the ward of O.B. ＆ G.Y. contaminated or not were examined in“E”Univ. Hospital from July to August, 1979 by using thioglycollate broths and agar plates. The following results were obtained: 1. The average colony number of airborne microbes revealed as follows the pediatric ward (36 colonies), the internal ward (33 colonies), the ward of O.B. ＆ G.Y. (30 colonies), the ward of surgery (24 colonies), delivery-waiting room (11 colonies), and the delivery room (3 colonies). 2. The bacterial growth beforenoon differed from that of afternoon. Namely, the latter (24 colonies) was higher than the former (21 colonies). 3. The type of strains isolated from the air of the ward revealed staphylococci (82%), Gram negative bacilli (18%), fungi (17%), Gram positive diplococci (13%), and Bacillus subtilis (2.8%). 4. The strains isolated in the delivery-waiting room revealed staphylococci (66.7%), Gram negative bacilli (33.6%), and revealed staphylococci (75%), Gram positive diplococci (8.3%), and fungi (8.3%), in delivery room. 5. Most of strains isolated in the ward of O.B. & G.Y. revealed staphylococci (100.0%), Gram positive diplococci (8.3%), and Gram negative bacilli (6.7%). 6. The strain isolated in the surgical ward revealed staphylococci (91.7%), fungi (33.3%), Gram positive diplococci (25%), Gram negative bacilli (25%) and Bacillus subtilis (8.3%). 7. The strain isolated in the pediatric ward revealed staphylococci (75%), fungi (25%), Gram positive diplococci (8.3%), Bacillus subtilis (8.3%), and Gram negative bacilli (8.3%). 8. The strain isolated in the internal ward revealed staphylococci (91.7%), fungi (33.3%), Gram positive diplococci (25%), and negative bacilli (16.7%). The strains isolated from the nasal cavity of those doctors and nurses who and enaged in the ward of O.B. & G.Y. revealed staphylococci (80%), Bacillus subtilis (10%), and Gram negative bacilli (10%), from doctors and Gram positive diplococci (10%), instead of Gram negative bacilli (10％), from nurses. 10. The strain isolated from nasal cavity of parturient women on admission revealed staphylococci (90%), and Gram negative bacilli (10%), but after admission revealed staphylococci (70％), Gram positive diplococci (10%), and Gram negative bacilli (10%). 11. Of the total 91 staphylococci isolated from the air of the ward, the Coagulase pastive was 36 (39.6%), and the negative 55 (60.4%), As a result of the coagulase experiment of the staphylococci isolated from the nasal cavity of those who are engaged in the ward of O.B. ＆ G.Y. all were revealed as negative that belonged to non-pathogenic. 12. Consequence of the biochemic examination of the gram negative bacilli isolated from the air of the ward the aerobacter aerogens revealed was (16.7%) E-coli 5％ in the nasal cavity of those came and went to the of O.B. ＆ G.Y. and Aerobacter aerogens 7.5%.

• 대한본초학회지
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• 제37권1호
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• pp.11-18
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• 2022

Objectives : Green gram (mung bean) has a cold nature and has been known to detoxify against various side effects that belong to hot in nature. In particular, since it has the effect of detoxifying fever and detoxification to treat swelling, it was also used externally to treat febrile dermatological diseases such as erysipelas and rubella. This study was designed to determine whether green gram exhibits anti-inflammatory effects on contact dermatitis in mice. Methods : We investigated the effects of green gram extract (70% ethanol extract) on skin lesion, skin thickness and weights, melanin and erythema index and spleen body weight ratio in mice with contact dermatitis induced by repeated application of 1-Fluoro-2,4-dinitrobenzene. Results : Topical application of green gram extract ameliorates skin lesions of contact dermatitis such as scale and roughness induces by 1-Fluoro-2,4-dinitrobenzene. green gram extract also suppressed enlargement of skin thicknesses and weights significantly. In addition, green gram extract treatment also lowered erythema index significantly compared to those in the control group. In the histopathological observation, green gram extract prevented epidermal hyperplasia and hyperkeratosis in inflamed tissues. Finally, green gram extract did not affect changes in body weights and the spleen body weight ratio, unlike dexamethasone, which significantly prevented body weight gain and lowered the spleen body weight ratio. Conclusions : These results imply that green gram, which is known to have a detoxifying effect in Korean medicine, can be used in the treatment of contact dermatitis.

• 한국환경보건학회지
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• 제8권1호
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• pp.25-30
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• 1982

The incidence of hospital infection has been seriously increased in the general hospital in recent years. This study was performed on hospital air and materials in a General Hospital in Seoul from June to December in 1980. The results were as follows: 1. Air sampling was done in multiple strategic areas by exposing standard petridishes for 5 minutes. There was a significant difference of airborne microbe between places. ($F._{99}$ = 3.2, p < 0.01). 2. The mean colony count was 8.6$\pm$6.2 colonies / plate / 5 minutes. 3. Gram stains of colony in air sampling were Gram (+) cocci 66.5%. Gram (+) rod 18.4%, Gram (-) cocci 1.3%, Gram (-) rod 8.7% Fungus 4.5%. 4. For the evaluation of sterilization of steam sterilizer and ethylene oxide gas sterilizer, biological monitoring were done by commercial spore strip. Positive culture was obtained in 2 out of 41 tests on 3 steam sterilizers, and in 3 out of 13 tests on ethylene oxide gas sterilizer. 5. Product sampling and culture were done for 2 kinds of disinfectants and 30 sets of various operation package or dressing materials. Positive culture was obtained in one disinfectants.

• 한국군사과학기술학회지
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• 제14권4호
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• pp.719-725
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• 2011

To apply TMAH-based Py-MS to a field biological detection system for real-time classification of cell-type, reproducible patterns of the TMAH-based Py-MS spectra was known as a critical factor for classification but was seriously disturbed by quantity of cells injected into pyro-tube. This factor is an exterior variable that could not be complemented by improving the performance of the TMAH-based Py-MS instrument. One of idea to solve the knotty problem has been flashed from "Top-down proteomics for identification of intact microoganisms". That is, biomarker peaks are selected from complicate Py-MS spectra for intact microoganisms by tracing out their origins, based on Py-MS spectra for the featured components of different cell-types, in Top-down approach. This idea has been tested in classification of different Gram-type microoganisms. Through the analyses of spectra for the featured components - peptidoglycan and lipoteichoic acid for Gram-positive cells and lipopolysaccharide and lipid A for Gram-negative cells - with comparing to the spectra the corresponding Gram-type cells in the Top-down approach, biomarker peaks were selected to carry out PCA(Principal Component Analysis) in order to see classification of different Gram-types, resulting in significant improvement of their classification. Furthermore, weighting biomarker peaks on intact cell's spectra, based on the data for the featured components of the Gram-types, contributed to elevate classification performance.

• 한국임상수의학회지
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• 제14권2호
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• pp.215-222
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• 1997

Enrofloxacin-colistin combination, widely used in Gram negative infections in veterinary sector, was investigated in terms of MIC and initial killing rate using E coli k 88ab, Salmonella typhimurium, Pasteurella multocida type A, Bordetella bronchiseptica and Staphylococcus aureus as test organisms. On the basis of MICs of enrofloxacin-colistin combination against the above bacteria, killing rates of the combination of enrofloxacin and colistin at the ratio of 5:0, 4:1, 3:2, 1:1, 2:3, 1:4 and 0:5, indicated high and rapid antibacterial acitivities against all but Staphylococcus aureus R-209, with the number of bacteria reducing to less than one percent within two hours. At the MIC of enrofloxacin or colistin, both antibacterials showed the highest killing rates during 2-4 hours against Gram negatives such as E coli K88ab,Pasteurella multocida type A and Bodetella bronchiseptica but allowed the regrowth of the same pathogens thereafter. On the while, the combination of two antibacterials at a fourth MIC resulted in high killing rate without bacterial regrowth during 24 hours, suggesting the synergistic antivacterial effects. The combination, however, did not show favourable activity against Gram negatime S typhimurium and Gram positive S aureus ergistic antibacterial activity against Gram negatime pathogens but also colistin showed LPS-neutraization, we could suggest the combination should provide clinically positive therapeutic armarium in Gram negative infections.