• 제목/요약/키워드: 3P

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담자균(擔子菌)이 생산(生産)하는 효소(酵素)에 관(關)한 연구(硏究) -제2보(第二報) Cellulase 및 Xylanase의 성질(性質)- (Studies on the Enzymes Produced by Basidiomycetes -Part II. Properties of Cellulase and Xylanase-)

  • 홍재식;권용주
    • Applied Biological Chemistry
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    • 제24권4호
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    • pp.260-266
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    • 1981
  • Pleurotus ostreatus 301과 Lentinus edodes 3-1을 볏짚배지(培地)에 배양(培養)하여 배양중(培養中)에 생성(生成)된 cellulase와 xylanase의 성질(性質)을 검토(檢討)한 결과(結果) P. ostreatus 301의 cellulase 활성(活性)은 기질농도(基質濃度) 0.6%, L. edodes 3-1은 0.8%, xylanase는 양균주(兩菌株)에서 1%까지는 비례적(比例的)으로 증가(增加)하였다. 반응시간(反應時間)에 따른 환원당(還元糖)의 생성(生成)은 양균주(兩菌株)에서 cellulase는 60분(分), xylanase는 30분(分)까지 비례적(比例的)으로 증가(增加)하였다. 최적(最適) pH는cellulase에서 P. ostreatus 301은 pH 4.0, L. edodes 3-1은 pH 4.5이었으며 xylanase는 양균주(兩菌株)가 pH 5.0이었다. pH 안정범위(安定範圍)는 P. ostreatus 301의 cellulase에서 pH $4.0{\sim}6.0$, L. edoder 3-1은 pH $3.0{\sim}5.0$이었고 xylanase는 P. osteatus 301에서 pH $4.5{\sim}6.0$, L. edodes 3-1에서는 pH $3.5{\sim}6.0$이었다. 최적온도(最適溫度)는 P. ostreatus 301의 cellulase에서 $40^{\circ}C$, L. edodes 3-1의 xylanase는 $45^{\circ}C$이었고, L. edodes 3-1의 cellulas와 P. ostreatus 301의 xylanase는 $50^{\circ}C$ 이었으며 열안정성(熱安定性)은 최적온도(最適溫度) 이하이었고, $70^{\circ}C$, 10분(分)으로 대부분 실활(失活)되었다. 염류(鹽類)의 영향(影響)은 L. edodes 3-1의 cellulase는 $Co^{++},\; Mg^{++}$에 의해서 효소력(酵素力)이 증가(增加)되었으며,$Mg^{++},\;Cu^{++}$에 의해서는 양균주(兩菌株)에서 심한 조해(阻害)를 를 보였고 xylanase는 $Ca^{++},\;Co^{++},\;Mg^{++}\;Cd^{++}$ 등은 약간 증가(增加)를 보였고, $Hg^{++}$은 조해(阻害)가 심했다.

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AlGaP2 반도체내와 표면의 Mn에 대한 제일원리 해석 (A First Principles Study of Mn on AlGaP2 Semiconductor Surface)

  • 강병섭
    • 반도체디스플레이기술학회지
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    • 제20권1호
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    • pp.12-17
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    • 2021
  • The electronic and magnetic properties for Mn-adsorbed on the chalcopyrite (CH) AlGaP2 semiconductor are investigated by using first-principles FPLMTO method. The clean CH-AlGaP2 without adsorbed Mn is a p-type semiconductor with a direct band-gap. The Mn-adsorbed CH-AlGaP2 exhibits the ferromagnetic state. It is more energetically stable than the other magnetic ones. The interstitial site on P-terminated surface is more energetically favorable one than the Al/Ga-terminated surface, or the other adsorbing sites. In the case of Mn-adsorbed Al/Ga-terminated surface, it is induced a strong coupling between Mn-3d and neighboring P-3p electrons. The holes of partially unoccupied minority Mn-3d state and majority (or minority) Al-3p or P-3p state are induced. Thus a high magnetic moment of Mn is sustained by holes-mediated double-exchange coupling. It is noticeable that the semiconducting and half-metallic characteristics of CH-AlGaP2:Mn thin film is disappeared.

NEW CONGRUENCES WITH THE GENERALIZED CATALAN NUMBERS AND HARMONIC NUMBERS

  • Elkhiri, Laid;Koparal, Sibel;Omur, Nese
    • 대한수학회보
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    • 제58권5호
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    • pp.1079-1095
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    • 2021
  • In this paper, we give new congruences with the generalized Catalan numbers and harmonic numbers modulo p2. One of our results is as follows: for prime number p > 3, $${\sum\limits_{k=(p+1)/2}^{p-1}}\;k^2B_{p,k}B_{p,k-(p-1)/2}H_k{\equiv}(-1)^{(p-1)/2}\(-{\frac{521}{36}}p-{\frac{1}{p}}-{\frac{41}{12}}+pH^2_{3(p-1)/2}-10pq^2_p(2)+4\({\frac{10}{3}}p+1\)q_p(2)\)\;(mod\;p^2),$$ where qp(2) is Fermat quotient.

Purification and Characterization of 2,3-Dihydroxybiphenyl 1,2- Dioxygenase from Comamonas sp.

  • Lee Na Ri;Kwon Dae Young;Min Kyung Hee
    • 한국미생물학회:학술대회논문집
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    • 한국미생물학회 2001년도 추계학술대회
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    • pp.16-25
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    • 2001
  • A genomic library of biphenyl-degrading strain Comamonas sp. SMN4 was constructed by using the cosmid vector pWE15 and introduced into Escherichia coli. Of 1,000 recombinant clones tested, two clones that expressed 2,3-dihydroxybiphenyl 1,2-dioxygenase activity were found (named pNB 1 and pNB2). From pNB1 clone, subclone pNA210, demonstrated 2,3-dihydroxybiphenyl 1,2-dioxygenase activity, is isolated. 2,3-Dihydroxybiphenyl 1,2-dioxygenase (23DBDO, BphC) is an extradiol-type dioxygenase that involved in third step of biphenyl degradation pathway. The nucleotide sequence of the Comamonas sp. SMN4 gene bphC, which encodes 23DBDO, was cloned into a plasmid pQE30. The His-tagged 23DBDO produced by a recombinant Escherichia coli, SG 13009 (pREP4)(pNPC), and purified with a Ni-nitrilotriacetic acid resin affinity column using the His-bind Qiagen system. The His-tagged 23DBDO construction was active. SDS-PAGE analysis of the purified active 23DBDO gave a single band of 32 kDa; this is in agreement with the size of the bphC coding region. The 23DBDO exhibited maximum activity at pH 9.0. The CD data for the pHs, showed that this enzyme had a typical a-helical folding structures at neutral pHs ranged from pH 4.5 to pH 9.0. This structure maintained up to pH 10.5. However, this high stable folding strucure was converted to unfolded structure in acidic region (pH 2.5) or in high pH (pH 12.0). The result of CD spectra observed with pH effects on 23DBDO activity, suggested that charge transition by pH change have affected change of conformational structure for 23DBDO catalytic reaction. The $K_m$ for 2,3-dihydroxybiphenyl, 3-metylcatechol, 4-methylcatechol and catechol was 11.7 $\mu$M, 24 $\mu$M, 50 mM and 625 $\mu$M.

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High performance of inverted polymer solar cells

  • Lee, Hsin-Ying;Lee, Ching-Ting;Huang, Hung-Lin
    • 한국진공학회:학술대회논문집
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    • 한국진공학회 2015년도 제49회 하계 정기학술대회 초록집
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    • pp.61.2-61.2
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    • 2015
  • In the past decades, green energy, such as solar energy, wind power, hydropower, biomass energy, geothermal energy, and so on, has been widely investigated and developed to solve energy shortage. Recently, organic solar cells have attracted much attention, because they have many advantages, including low-cost, flexibility, light weight, and easy fabrication [1-3]. Organic solar cells are as a potential candidate of the next generation solar cells. In this abstract, to improve the power conversion efficiency and the stability, the inverted polymer solar cells with various structures were developed [4-6]. The novel cell structures included the P3HT:PCBM inverted polymer solar cells with AZO nanorods array, with pentacene-doped active layer, and with extra P3HT interfacial layer and PCBM interfacial layer. These three difference structures could respectively improve the performance of the P3HT:PCBM inverted polymer solar cells. For the inverted polymer solar cells with AZO nanorods array as the electronic transportation layer, by using the nanorod structure, the improvement of carrier collection and carrier extraction capabilities could be expected due to an increase in contact area between the nanorod array and the active layer. For the inverted polymer solar cells with pentacene-doped active layer, the hole-electron mobility in the active layer could be balanced by doping pentacene contents. The active layer with the balanced hole-electron mobility could reduce the carrier recombination in the active layers to enhance the photocurrent of the resulting inverted polymer solar cells. For the inverted polymer solar cells with extra P3HT and PCBM interfacial layers, the extra PCBM and P3HT interfacial layers could respectively improve the electron transport and hole transport. The extra PCBM interfacial layer served another function was that led more P3HT moving to the top side of the absorption layer, which reduced the non-continuous pathways of P3HT. It indicated that the recombination centers could be further reduced in the absorption layer. The extra P3HT interfacial layer could let the hole be more easily transported to the MoO3 hole transport layer. The high performance of the novel P3HT:PCBM inverted polymer solar cells with various structures were obtained.

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Dephosphorylation of p53 Ser 392 Enhances Trimethylation of Histone H3 Lys 9 via SUV39h1 Stabilization in CK2 Downregulation-Mediated Senescence

  • Park, Jeong-Woo;Bae, Young-Seuk
    • Molecules and Cells
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    • 제42권11호
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    • pp.773-782
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    • 2019
  • Cellular senescence is an irreversible form of cell cycle arrest. Senescent cells have a unique gene expression profile that is frequently accompanied by senescence-associated heterochromatic foci (SAHFs). Protein kinase CK2 (CK2) downregulation can induce trimethylation of histone H3 Lys 9 (H3K9me3) and SAHFs formation by activating SUV39h1. Here, we present evidence that the PI3K-AKT-mTOR-reactive oxygen species-p53 pathway is necessary for CK2 downregulation-mediated H3K9me3 and SAHFs formation. CK2 downregulation promotes SUV39h1 stability by inhibiting its proteasomal degradation in a p53-dependent manner. Moreover, the dephosphorylation status of Ser 392 on p53, a possible CK2 target site, enhances the nuclear import and subsequent stabilization of SUV39h1 by inhibiting the interactions between p53, MDM2, and SUV39h1. Furthermore, $p21^{Cip1/WAF1}$ is required for CK2 downregulation-mediated H3K9me3, and dephosphorylation of Ser 392 on p53 is important for efficient transcription of $p21^{Cip1/WAF}$. Taken together, these results suggest that CK2 downregulation induces dephosphorylation of Ser 392 on p53, which subsequently increases the stability of SUV39h1 and the expression of $p21^{Cip1/WAF1}$, leading to H3K9me3 and SAHFs formation.

Pestalotiopsis sp. KCTC 8637P에 의한 세포외 생물고분자의 생산조건과 응집활성 (Culture Conditions and Flocculating Activity of Exo-biopolymer Produced by Pestalotiopsis sp. KCTC 8637p)

  • 문성훈;권기석
    • KSBB Journal
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    • 제11권4호
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    • pp.470-475
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    • 1996
  • 썩은 나무 잎에서 세포외 생물고분자 응집제를 생 산하는 흰 곰팡이를 분리하여 Pestalotiopsis sp. M01로 동정하였다. 이 균주가 생산하는 생물고분자 응집제의 응집활성에 대한 배양 조건을 무기염 배지인 Czapek-Dox 배지를 기초로 하여 탄소원, 질소 원, 배지의 pH멸, 그리고 옹도별로 조사하였다. 그 결과로서 응집활성 조건은 3 % sucrose, 0.3 % $KN0_3$, pH 7, 그리고 $25^{\circ}C$에서 최대 응집활성을 나타내었다. 반면에 이 균주의 생육 조건은 3% su-crose, 0.3 % $KN0_3$, pH 5, 그리고 $25^{\circ}C$에서 최대 생육을 나타내었다.

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포토리소그래피를 이용한 P3HT 활성층의 패터닝에 대한 연구 (Study on Photolithographic Patterning for P3HT Active Layer)

  • 박경동;남동현;박정환;한교용
    • 한국전기전자재료학회논문지
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    • 제20권4호
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    • pp.294-302
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    • 2007
  • We studied on possibility of the application of photolithography technique to patterning the organic active layer poly(3-hexylthiophene) (P3HT). In the case of selective etching method, we made thin oxide film on P3HT thin film using $O_2$ treatment. We achieved the field-effect mobilities in the saturation regime ${\sim}1.2{\times}10^{-3}\;cm^2/V{\cdot}s$, $I_{on/off}$ ratios ${\sim}10^5$ in the selective etching method, ${\sim}7.4{\times}10^{-4}cm^2/V{\cdot}s$, $I_{on/off}$ ratios ${\sim}5{\times}10^3$ in the lift-off one. These values are higher than ones of the unpatterned P3HT-based OTFTs. On the basis of the above results, we demonstrate the photolithographic patterning for P3HT active layer is successfully carried out without degradation of P3HT.

산채류를 이용한 음료 개발에 관한 연구 (Development of Beverages Drinks Using Mountain Edible Herbs)

  • 함승시;이상영;오덕환;김상헌;홍정기
    • 한국식품영양과학회지
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    • 제26권1호
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    • pp.92-97
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    • 1997
  • 산채 일반음료는 산채를 착즙하여 쥬스를 제조한 후 각종 부원료를 사용하여 최적의 배합비로 산채음료를 제조하였으며, 산채 발효음료는 Lactobacillus helveticus를 이용한 산채음료 원액과 시럽 믹스를 1 : 3으로 혼합한 다음, 균질기를 이용하여 균질화한 후 요구르트 향료를 첨가하여 발효음료를 제조하였다. 이 시제품들에 대한 일반분석 실험과 저장안정성 실험을 행하였다. 산채 일반음료의 일반분석 결과 37$^{\circ}C$에서 6개월간 저장시 수리취와 고려엉겅퀴음료의 pH는 각각 3.80과 3.7, 산도는 각각 0.50 및 0.49, OD값은 각각 1.021 및 1.119 로 나타났다. 그리고 저장안정성 시험은 상온 및 37$^{\circ}C$로 구분하여 6개월간 저장하면서 pH와 색도의 경시적 변화를 측정한 결과 상온에서의 pH의 변화는 수리취가 3.96~3.95, 고려 엉 겅퀴는 3.99~3.89로 각각 0.01과 0.1정도의 미미한 변화를 보였으며, 37$^{\circ}C$에서는 각각 3.95 ~3.83과 3.98~3.87로 0.12정도의 변화폭을 보였다. 산채 발효음료의 일반분석에서는 수리 취와 엉겅퀴의 pH는 각각 3.65와 3.70, 산도는 0.57 및 0.60, S.N.F.는 3.2 및 3.1 그리고 유산균수는 각각 2.5$\times$$10^{8}$ 과 4.0$\times$$10^{8}$ 으로 나타났다. 저장 안정성 실험은 4$^{\circ}C$에서 15일간 저장 하면서 pH와 산도의 경시적인 변화를 측정하였다. 수리취와 엉겅퀴의 pH는 3.56~3.39 및 3.67~3.48로 각 각 0.17과 0.19의 차이를 보였다.

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