• Korean Journal of Microbiology
• /
• v.52 no.3
• /
• pp.286-297
• /
• 2016

Clostridium perfringens is both a ubiquitous environmental bacterium and a major cause of human gastrointestinal disease, and C. perfringens food poisoning ranks among the most common gastrointestinal diseases in developed countries. 120 isolates of C. perfringens were obtained from food-poisoning outbreaks in 2013~2014, Gyeonggi-do. Using PCR, all 120 isolates were identified as C. perfringens type A. Of the tested isolates, 49 isolates carried the cpe gene, 71 isolates carried the cpb2 gene. The outbreak cases of cpb2 and cpe /cpb2 genes were 7 and 7, whereas the outbreak cases of cpe-gene were 2. The epidemiological relationship between C. perfringens isolates has previously been investigated chiefly by pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST). The genetics relatedness of the isolates raged from 53.5-100% and 75 district PFGE type were observed. The PFGE results revealed a wide genetic diversity among the 64 cpb2 carrying isolates (except 7 isolates), while 46 cpe-carrying isolates (except 3 isolates) showed a high genetic similarity. The MLST analysis revealed that 14 cpe isolates (cpe-chromosomal isolates) belong to a distinct cluster that is significantly distant from all the other cpb2 isolates (cpe-plasmid carrying and cpe-negative isolates). The isolates carrying a cpb2 appear to be rarely related, and are more variable than chromosomal cpe isolates. The results suggest that the cpe-positive outbreak isolates showed close genetic relation, whereas the cpb2-positive isolates revealed a wide genetic diversity.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
• /
• v.5 no.1
• /
• pp.70-82
• /
• 1994

Present study was to evaluate the validity and the clinical utility of the Korean version of Luria-Nebraska Neuropsychological Battery for Children(LNNB-C) in various groups including normal, brain damaged attention deficit hyperactivity disordered(ADHD), and psychiatrically disordered. The Korean version of LNNB-C and BGT were administered to clinical groups consisted of 51 patients(19 brain damaged, 16 ADHD. and 16 psychiatric controls), and to normal group composed of 147 children between the age of 8 and It Also KEDI-WISC was administered D clinical groups as a part of comprehensive psychological assessment There were significant differences between the brain damaged and the normals on all scales of LNNB-C, and between the normals and the ADHD on 11 clinical scales and 3 summary scales, which indicate the clinical validity for the scales of the Korean version of LNNB-C. The significant differences between the ADHD and the brain damaged on 3 summary scales were found, suggesting that the summary scales might play an important role id discriminating between two groups. Multiple discriminant analysis showed that the Korean version of LNNB-C significantly discriminates 3 groups - normals, ADHD, and brain damaged. Percentages of correct classification were ranged from 62.5% in the ADHD to 98.6Ta in the normals. For further evaluating the discriminant validity of the LNNB-C, the discriminant power of each items were calculated, and 131 of the 147 items discriminated significantly between the brain damaged and the normals. The scales of LNNB-C significantly correlated with the error scores of BGT and the most of scales of KEDI-WISC. These results put together : strongly support the concurrent and the discriminant validity of the Korean version of LNNB-C in diagnosing brain damage. The limitations of present study and several issues for the luther study were discussed.

• Clean Technology
• /
• v.20 no.1
• /
• pp.42-50
• /
• 2014

In this study, the $Mg(OH)_2$ slurry was made form ferro-nickel slag and then used for $CO_2$ sequestration. The experiments were in the order as leaching step, precipitation, carbonation experiments. According to the leaching results, the optimal leaching conditions were $H_2SO_4$ concentration of 1 M and the temperature of 333 K. In the $Mg(OH)_2$ manufacturing step, NaOH was added to increase the pH upto 8, the first precipitation was confirmed as $Fe_2O_3$. After removal the first precipitation, the pH was upto 11, the $Mg(OH)_2$ was generated by XRD analysis. The $Mg(OH)_2$ slurry was used for $CO_2$ sequestration. The pseudo-second-order carbonation model was used to apply for $CO_2$ sequestration. The $CO_2$ sequestration rate was increased by the $CO_2$ partial pressure and temperature. However, $CO_2$ sequestration rate was decreased when temperature upto 323 K. After $CO_2$ sequestrated by $Mg(OH)_2$, the $CO_2$ can be sequestrated stable as $MgCO_3$. This study also presented optimal sequestration condition was the pH upto 8.38, the maximum $MgCO_3$ can be generated. This study can be used as the basic material for $CO_2$ sequestration by ferro-nickel slag at pilot scale in the future.

• Food Science of Animal Resources
• /
• v.28 no.4
• /
• pp.422-430
• /
• 2008

This study was conducted to investigate the nutritional composition and meat quality properties of Hanwoo steer beef by different cut. Ten cuts [Abjin (short plate), Bosup (top sirloin), Cheggt (striploin), Dngsim (loin), Guri (chuck tender), Hongduke (eye of round), Moksim (chuck roll), Sulgit (bottom round), Udoon (top round), Yanaji (brisket)] prepared from 10 Hanwoo steers (28-30 months old) were used for this experiment. The range of pH was 5.46-5.64. In the chemical analysis, Hongduke contained highest protein contents (21.15%) and Dngsim had lowest protein contents (16.94%) (p<0.05). Fat contents were higher in Dngsim whereas those of Guri, Hongduke and Udoon were significantly lower when compared to the other cuts (p<0.05). There were not significantly different in meat color (L, a and b values) properties only except that L values were significantly higher in Dngsim among 10 cuts (p<0.05). Cooking loss (%) was higher in Guri, Hongduke, Udoon and Yangji whereas it was significantly love. in Cheggt (p<0.05). Warner-Bratzler shear force (WBS) was significantly higher in Abjin $(6.24inch^2/kg)$, whereas Dngsim $(3.45inch^2/kg)$ and Cheggt $(3.50inch^2/kg)$ were significantly lower than the other cuts (p<0.05). There was no significant difference in water-holding capacity (WHC) among 10 cuts (p>0.05). Total amino acid contents were significantly higher in Hongduke (p<0.05). Hongduke was highest in most kinds of amino acids, however, Udoon had the highest methionine and histidine contents among 10 cuts (p<0.05). Glutamate contents were high in all cuts and followed by aspartate, leucine and lysin. With regard to mineral contents, Ca was ranged from 47.63-70.69 mg/kg and Fe was ranged from 15.09-26.68 mg/kg in 10 cuts. Zn was highly contained in Guri (50.56 mg/kg) when compared to the other cuts (p<0.05).

• Journal of The Geomorphological Association of Korea
• /
• v.19 no.3
• /
• pp.1-22
• /
• 2012

The classification scheme of estuaries can be divided into two categories: qualitative classification based on geomorphic characteristics and quantitative classification based upon the physical properties of water body. While simple and intuitive scheme of the former is difficult to quantify, the latter is not easy to apply due to the lack of data. A classification scheme based on morphological convergence is very promising because it only requires easily accessible data such as width and depth of channels, as well as it can characterize estuaries in terms of tidal propagation. Thus, this paper examines the classification scheme based on estuarine morphological convergence using depth and width data obtained from 19 major Korean estuaries. Morphological convergence for each estuary was estimated with the estuarine length, width and depth data to get the convergence parameters, which includes the degree of funneling ${\nu}$ and the dimensionless estuarine length $y_0$. The transfer function ${\xi}({\nu},ky)$ is then deduced analytically from 1D depth-integrated hydrodynamic momentum equation and continuity equation for estuarine shapes. Tidal response of each estuary is finally calculated using ${\nu}$, $y_0$ and ${\xi}({\nu},ky)$ for comparison and classification. The 19 Korean estuaries were classified into three groups: tidal amplitude-dominated estuaries with standing wave-like tidal response (group 1), current-dominated estuaries with progressive wave-like tidal response (group 2), and the intermediate group (group 3) between groups 1 and 2. The sensitivity analysis revealed that uncertainties in determining the estuarine length can have a critical effect upon the results of classification, which indicates that the reasonable determination of the estuarine length is of critical importance. Once the estuarine length is feasibly determined, depth-convergence can be neglected without any negative effect on the classification scheme, which has an important ramification on the wide applicability of the classification scheme.

• Korean Journal of Soil Science and Fertilizer
• /
• v.18 no.4
• /
• pp.325-335
• /
• 1985

Biogas production from agricultural wastes were summarized as follows: 1. Biogas Generation Characteristics of Various Manures and Residues a. Gas yield from crop residues like rice straw, rice hull, corn stalk and coconut husk can be improved by addition of animal manures. b. Gas yield from coconut husk can be improved through aerobic fermentation for at least one week before loading in the digester. c. Gas yield from fresh rice straw is better than from pre-fermented one, whether alone or in combination with animal manures. d. Initial study has shown that fresh azolla can be substituted for animal manures in manurerice straw combinations and gas yield derived based on unit volatile solids loaded is actually better than for manure-residue combinations. e. Gas production is highly sensitive to substrate pH and becomes almost nil at a pH of below 6. 2. Effect of ambient conditions and other factors on biogas production in a house hold-size digester. a. Results showed that compaction of rice straw in straw-manure combination can reduce gas yield compared with loosely mixed straw. b. The effective gas production period extended to 70 days using freshly threshed rice straw and fresh cattle manure as feed material. c. Underground and above ground digesters with shade have relatively more stable substrate temperature than aboveground exposed digesters. This relative temperature instability may likely be the reason for lower gas yield for the exposed aboveground digester loaded with loose straw-cattle manure substrate, compared with the underground digester with the same substrate. 3. Economic Analysis a. Based on prevailing costs of fuel, materials, and labor in the Philippines, biogas produced from the household size system is cheaper than either LPG or kerosene. b. If other benefits like organic fertilizer, pollution control and convenience are considered, biogas will surely be the best alternative fuel source.

• Journal of Practical Agriculture & Fisheries Research
• /
• v.23 no.2
• /
• pp.43-50
• /
• 2021

Growth tests on the Yuldo, Supum1 and Supum2 cultivars of Pyropia dentata were performed at the Eoran and Imha aquafarm, Haenam in Jeollanamdo, from October to December in 2017. The mean water temperature ranged from 5.4 to 26.4 ℃. In Eoran aquafarm (flating raft method), dissolved total nitrogen (DTN), dissolved total phosphorus (DTP), total nitrogen (TN), total phosphorus (TP) and chemical oxygen demand (COD) ranged from 0.091 to 0.181 mg/L, 0.007 to 0.019 mg/L, 0.114 to 0.187 mg/L, 0.008 to 0.033 mg/L and 0.200 to1.000 mg/L, respectively. In Imha aquafarm (fixed pold method), DTN, DTP, TN, TP and COD ranged from 0.118 to 0.276 mg/L, 0.005 to 0.024 mg/L, 0.155 to 0.305 mg/L, 0.009 to 0.042 mg/L and 0.300 to1.400 mg/L, respectively. In order to investigate the number of conchospores attached on the Pyropia net, which was cut into about 4cm long. The mean number of conchospores of Yuldo, Supum1 and Supum2 cultivars were 39.5, 26.5, 72.5, respectively. Young thalli were harvested two times at Eoran aquafarm, and three times at Imha aquafarm. In eoran aquafarm, the mean thallus length of Yuldo, Supum1 and Supum2 cultivars were 49.9, 46.0, 42.0 cm on October and 163, 126.0, 263.0 cm on November, respectively. The mean thallus width of Yuldo, Supum1 and Supum2 cultivars were 5.8, 4.6, 11.5 cm on October and 20.9, 11.5, 14.0 cm on November, respectively. In Imha aquafarm, the mean thallus length of Yuldo, Supum1 and Supum2 cultivars were 119.0, 60.9, 71.0 cm on October, 196.0, 132.0, 262.0 cm on November and 187.0, 281.0, 296.0 cm on December, respectively. The mean thallus width of Yuldo, Supum1 and Supum2 cultivars were 4.2, 3.4, 3.1 cm on October, 8.9, 6.2, 6.6 cm on November and 11.7, 11.6, 9.4 cm on December, respectively. In eoran aquafarm, contents of moisture, crude ash, crude lipid, crude protein and carbohydrate of three cultivars ranged from 11.64 to 20.15, 19.54 to 21.19, 0.00 to 0.18, 29.78 to 37.81, 29.16 to 29.71, respectively. In Imha aquafarm, contents of moisture, crude ash, crude lipid, crude protein and carbohydrate of three cultivars ranged from 8.43 to 9.15, 11.42 to 17.49, 0.00 to 0.00, 31.90 to 37.54, 36.30 to 42.24, respectively.

• Journal of Food Hygiene and Safety
• /
• v.38 no.5
• /
• pp.361-372
• /
• 2023

Organotin pesticide is used as an acaricide in agriculture and may contaminate livestock products. This study aims to develop a rapid and straightforward analytical method for detecting organotin pesticides, specifically azocyclotin, cyhexatin, and fenbutatin oxide, in various livestock products, including beef, pork, chicken, egg, and milk, using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The extraction process involved the use of 1% acetic acid in a mixture of acetonitrile and ethyl acetate (1:1). This was followed by the addition of anhydrous magnesium sulfate (MgSO₄) and anhydrous sodium chloride. The extracts were subsequently purified using octadecyl (C₁₈) and primary secondary amine (PSA), after which the supernatant was evaporated. Organotin pesticide recovery ranged from 75.7 to 115.3%, with a coefficient of variation (CV) below 25.3%. The results meet the criteria range of the Codex guidelines (CODEX CAC/GL 40). The analytical method in this study will be invaluable for the analysis of organotin pesticides in livestock products.

• Restorative Dentistry and Endodontics
• /
• v.36 no.6
• /
• pp.490-497
• /
• 2011

Objectives: This study examined the effect of the uncured dentin adhesives on the bond interface between the resin inlay and dentin. Materials and Methods: Dentin surface was exposed in 24 extracted human molars and the teeth were assigned to indirect and direct resin restoration group. For indirect resin groups, exposed dentin surfaces were temporized with provisional resin. The provisional restoration was removed after 1 wk and the teeth were divided further into 4 groups which used dentin adhesives (OptiBond FL, Kerr; One-Step, Bisco) with or without light-curing, respectively (Group OB-C, OB-NC, OS-C and OS-NC). Pre-fabricated resin blocks were cemented on the entire surfaces with resin cement. For the direct resin restoration groups, the dentin surfaces were treated with dentin adhesives (Group OB-D and OS-D), followed by restoring composite resin. After 24 hr, the teeth were assigned to microtensile bond strength (${\mu}TBS$) and confocal laser scanning microscopy (CLSM), respectively. Results: The indirect resin restoration groups showed a lower ${\mu}TBS$ than the direct resin restoration groups. The ${\mu}TBS$ values of the light cured dentin adhesive groups were higher than those of the uncured dentin adhesive groups (p < 0.05). CLSM analysis of the light cured dentin adhesive groups revealed definite and homogenous hybrid layers. However, the uncured dentin adhesive groups showed uncertain or even no hybrid layer. Conclusions: Light-curing of the dentin adhesive prior to the application of the cementing material in luting a resin inlay to dentin resulted in definite, homogenous hybrid layer formation, which may improve the bond strength.

• Korean Journal of Adult Nursing
• /
• v.12 no.1
• /
• pp.99-111
• /
• 2000

The purpose of this study was to identify risk factors related to sexual function of women with coronary artery disease, and to determine the predictors of sexual function. The study design, a descriptive correlational study, was done through structural questionnaire and interview. A total of 50 subjects from C University Hospital at Kwang-ju city who have undergone coronary angiography at department of cardiology were observed and interviewed from Feb. 22, 1999 to March. 23, 1999. The number of affected vessels, the level of total serum cholesterol, and the ejection fraction of 2-D echo cardiography were analyzed to evaluate the severity of coronary artery disease. And also type A behavior pattern, health behavior, Brief Index of Sexual Functioning for Women (BISF-W) were measured. The data obtained were analyzed using percentage, mean and standard deviation, t-test, ANOVA, Pearson's correlation coefficient, and stepwise multiple regression analysis via SPSS PC+. The results of this study were as follows: 1. The mean age of the subjects were 58.1 and 72.0% of those have been married over 30 years. Seventy two percentage were unemployed and monthly family income of 56.6% was less than 1,000,000 won (approximately $ 840). Eighty percent were in their postmenopausal state, and the frequency of sexual intercourse of 84.0% were two to three times per month. 2. The scores of type A behavior pattern were from 16 to 38(mean 24.94) and health behavior ranged from 21 to 43(mean 31.2). Abstinence from smoking, alcohol, and caffeine were best compliant factors and weight control and exercise were least abided ones. The result of 2D-ECHO EF showed that the half of the subjects were abnormal, and 24% had more than 240mg/dl of total serum cholesterol. The coronary angiography showed that 64% of the subjects had more than one affected vessels. 3. The predictors to explain the factor score of 'orgasm' were number of health examination, the pre- or post-menopausal state, protestant, number of coronary vessel affected, level of serum total cholesterol, and comorbid group of hypertension and diabetes, and it's total variance accounted for 52.4%. The predictors to explain the factor score of 'sexual activity' were comorbid group of hypertension and diabetes and type A behavior pattern, which accounted for 22.4% of total variance. The predictors to explain the factor score of 'sexual satisfaction' were type A behavior pattern, no religion, exercise, level of serum total cholesterol, and pre or post menopausal state, which accounted for 52.1%. The predictors to explain the factor score of 'sexual desire' were the period of marriage, type A behavior, employment or unemployment, and weight control, which accounted for 43.2%. The predictors to explain the factor score of 'external force of sexual functioning' were physical overload and exercise, which accounted for 41.1%. The predictors to explain the factor score of 'sexual activity' were family monthly income, catholics, and exercise, and which accounted for 35.4%. Above results lead us to some consensus that sexual function of women with coronary artery disease is related to various factors including vasogenic factors such as total serum cholesterol level, number of coronary vessel affected, an endocrinal factor such as menopausal state, and type A behavior pattern as a sociopshychological factor. And also health behaviors such as fitness care, overwork, weight control, and emotional tension are contributed to sexual function.