• Korean Journal of Plant Resources
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• v.29 no.3
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• pp.297-304
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• 2016

The seed of safflower (Carthamus tinctorius L) has been reported to suppress human cancer cell proliferation. However, the mechanisms by which safflower seed inhibits cancer cell proliferation have remained nuclear. In this study, the inhibitory effect of the safflower seed (SS) on the proliferation of human colorectal cancer cells and the potential mechanism of action were examined. SS inhibited markedly the proliferation of human colorectal cancer cells (HCT116, SW480, LoVo and HT-29). In addition, SS suppressed the proliferation of human breast cancer cells (MDA-MB-231 and MCF-7). SS treatment decreased cyclin D1 protein level in human colorectal cancer cells and breast cancer cells. But, SS-mediated downregulated mRNA level of cyclin D1 was not observed. Inhibition of proteasomal degradation by MG132 attenuated cyclin D1 downregulation by SS and the half-life of cyclin D1 was decreased in SS-treated cells. In addition, SS increased cyclin D1 phosphorylation at threonine-286 and a point mutation of threonine-286 to alanine attenuated SS-mediated cyclin D1 degradation. Inhibition of ERK1/2 by PD98059 suppressed cyclin D1 phosphorylation and downregulation of cyclin D1 by SS. In conclusion, SS has anti-proliferative activity by inducing cyclin D1 proteasomal degradation through ERK1/2-dependent threonine-286 phosphorylation of cyclin D1. These findings suggest that possibly its extract could be used for treating colorectal cancer.

• Korean Journal of Plant Resources
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• v.30 no.6
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• pp.640-646
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• 2017

In this study, we elucidated anti-cancer activity and potential molecular mechanism of 70% ethanol extracts from Taxilli Ramulus (Taxillus chinensis (DC.) Danser) (TR-E70) against human colorectal cancer cells. Anti-cell proliferative effect of TR-E70 was evaluated by MTT assay. The effect of TR-E70 on the expression of cyclin D1 in the protein and mRNA level was evaluated by Western blot and RT-PCR, respectively. TR-E70 suppressed the proliferation of human colorectal cancer cell lines, HCT116 and SW480. Although TR-E70 decreased cyclin D1 expression in protein and mRNA level, decreased level of cyclin D1 protein by TR-E70 more dramatically occurred than that of cyclin D1 mRNA. Cyclin D1 downregulation by TR-E70 was attenuated in presence of MG132. In addition, TR-E70 phosphorylated threonine-286 (T286) of cyclin D1. TR-E70-mediated cyclin D1 degradation was blocked in presence of LiCl as an inhibitor $GSK3{\beta}$ but not PD98059 as an ERK1/2 inhibitor and SB203580 as a p38 inhibitor. Our results suggest that TR-E70 may downregulate cyclin D1 as one of the potential anti-cancer targets through $GSK3{\beta}$-dependent cyclin D1 degradation. From these findings, TR-E70 has potential to be a candidate for the development of chemoprevention or therapeutic agents for human colorectal cancer.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2003.07b
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• pp.1196-1199
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• 2003

Recently, the development of diagnosis technique with high confidence is important on power equipment, for this reason is use for measurement and analysis of PD with prior appearance of insulation breakdown. In this paper, we presents the analysis of trouble signal to use ${\phi}-Q$ method which display 2D and 3D Equipment of simulation has made independently DS for 170kV GIS of one phase with same on field. The detected signal through the sensor of Induction and Acoustic Emission is classified to use characteristic neural network algorithm and then it is analysis.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.61 no.12
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• pp.1903-1910
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• 2012

This paper presents a data based low-order controller design algorithm for a linear time-invariant process with a time delay. The algorithm is composed by combining an identification step based on open loop pulse test with a low-order controller design step to obtain the entire set of controllers achieving multiple performance specifications. The initial information necessary for this algorithm are merely the width and amplitude of a rectangular pulse, a controller of four types (PI, PD, PID, first-order), and design objectives. Various parametric approaches that have been developed are merged in the controller design algorithm. The resulting controller set satisfying the design objectives are displayed on the 2D and 3D graphics and thus it is very easy for us to pick a controller inside the admissible set because we can check the corresponding closed-loop performances visually.

• Proceedings of the KIEE Conference
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• 2003.07c
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• pp.1759-1761
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• 2003

Recently, the development of diagnosis technique with high confidence is important on power equipment, for this reason is use for measurement and analysis of PD with prior appearance of insulation breakdown In this paper, we presents the analysis of trouble signal to use ${\Phi}$-Q method which display 2D and 3D Equipment of simulation has made independently DS for 170kV GIS of one phase with same on field. The detected signal through the sensor of Induction and Acoustic Emission is classified to use characteristic neural network algorithm and then it is analysis.

• Korean Journal of Optics and Photonics
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• v.5 no.1
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• pp.166-172
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• 1994

The fiber optic receiver, ETRI APD-FET 1.0, is developed for the application of optical communication. This fiber optic receiver includes PD sub-module and pre-amplifier case. A single lens system is introduced for the PD sub-module. The sub-module consists of the avalenche photodiode(APD), GRIN rod lens, and a single mode fiber. The above components are enclosed into the stainless steel 304L housings. By bevelling the fiber end, the single mode fiber provides less than ~ 28 dB of optical return loss. The area of image focus is controlled by adjusting the length of spacer located in-between the fiber and the GRIN rod lens. The laser welding technique is applied to achieve the maximum coupling efficiency for the joining of each housing. In the pre-amplifier case, GaAs FET pre-amplifier workes for photocurrent amplification and the thermister is mounted to control the APD bias. The performance of ETRI APD-FET1.0 shows the sensitivity of - 30.3 dBm at $10^{-10}$ BER(bit error rate) and 2.5 Gbps optical random signal of $2^{23}-1$ word length. The fiber optic receiver is one of the essensial parts of the transmission module for B-ISDN. Also, the above optical packaging technology will be adapted for the developement of 10 Gbps transmission application 2.5 Gbps 5 Gbps

• Journal of Korean Neurosurgical Society
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• v.56 no.5
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• pp.383-389
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• 2014

Objective : Neural tissue transplantation has been a promising strategy for the treatment of Parkinson's disease (PD). However, transplantation has the disadvantages of low-cell survival and/or development of dyskinesia. Transplantation of cell aggregates has the potential to overcome these problems, because the cells can extend their axons into the host brain and establish synaptic connections with host neurons. In this present study, aggregates of human brain-derived neural stem cells (HB-NSC) were transplanted into a PD animal model and compared to previous report on transplantation of single-cell suspensions. Methods : Rats received an injection of 6-OHDA into the right medial forebrain bundle to generate the PD model and followed by injections of PBS only, or HB-NSC aggregates in PBS into the ipsilateral striatum. Behavioral tests, multitracer (2-deoxy-2-[$^{18}F$]-fluoro-D-glucose ([$^{18}F$]-FDG) and [$^{18}F$]-N-(3-fluoropropyl)-2-carbomethoxy-3-(4-iodophenyl)nortropane ([$^{18}F$]-FP-CIT) microPET scans, as well as immunohistochemical (IHC) and immunofluorescent (IF) staining were conducted to evaluate the results. Results : The stepping test showed significant improvement of contralateral forelimb control in the HB-NSC group from 6-10 weeks compared to the control group (p<0.05). [$^{18}F$]-FP-CIT microPET at 10 weeks posttransplantation demonstrated a significant increase in uptake in the HB-NSC group compared to pretransplantation (p<0.05). In IHC and IF staining, tyrosine hydroxylase and human ${\beta}2$ microglobulin (a human cell marker) positive cells were visualized at the transplant site. Conclusion : These results suggest that the HB-NSC aggregates can survive in the striatum and exert therapeutic effects in a PD model by secreting dopamine.

• Transactions of the Korean Society of Mechanical Engineers
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• v.11 no.3
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• pp.362-367
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• 1987

Cylinder pressure level is defined the value of sound pressure level derived from relations of pressure and frequency which are obtained Fourier series expansion of cylinder pressure. CPL is alone in general use in combustion analysis without regarding attenuation coefficient determined by engine structure because it is almost constant in various kinds of engines. Combustion pressure fluctuation and influence of CPL was investigated in this paper using Wiebe combustion function superposition th obtain the effect of premixed and diffusive combustion. The results are as follow. (1) Influence of combustion maximum pressure(P$\sub$max/) on CPL is correspond with total energy per cycle well. (2) P$\sub$max/, .DELTA.P$\sub$max/, (dp/d.theta.)$\sub$max/ and (d$\^$2/p/d.theta.$\^$2/)$\sub$max/ are very effective on CPL in the range of low frequency, mid-low frequency, mid-high frequency and high frequency respectively.

• 제어로봇시스템학회:학술대회논문집
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• 2005.06a
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• pp.2164-2169
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• 2005

SFFS(Solid Freeform Fabrication System) can quickly makes models and prototype parts from 3D computer-aided design (CAD) data. Three dimensional printing(3DP) is a kind of the solid freeform fabrication. The 3DP process slices the modeling data into the 50-200um along to z axis. And we pile the powder and make the manufactures. A manufacture is made by the SFFS has the precision of the 50um. Therefore the x-y table of SFFS to move a printhead must be the system that has a high speed and accuracy. So we proposed the SMCSPO algorithm for SFFS. The major contribution is the design of a robust observer for estimating the state and the perturbation of the timing belt system, which is combined with a robust controller. The control performance of the proposed algorithm is compared with PD control by the simulation and the experiment. The control algorithm of the SFFS is presented in the office environment. The system between control system and printhead for the SFFS is also integrated

• Journal of Oriental Neuropsychiatry
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• v.23 no.4
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• pp.123-132
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• 2012

Objectives : This study aims to investigate the correlation between the characteristics of personality on MMPI scale, and the siblings relation, number of current family living with, gender and age of the Oriental Medical University students. Methods : For this study, we carried out the MMPI profiling and gathered information regarding the sibling relation of the subjects. We studied the correlation between the two measures, and the correlation between MMPI scales and the subjects' gender and age. Results : 1. The group without siblings had higher STY figures then the groups with siblings. 2. There was no significant differences in MMPI profiles among the only child group, first-born group, second-born group, and third-born group. 3. The married group showed higher Pd, Pt, Sc, BDL figures than the unmarried group. 4. As the number of family members currently living with increased, the BDL and STY figures decreased. 5. The male group had higher Hs, Hy, Pa, Ma, NAR, BDL, ANT, CPS, PAR, and SZD figures, while the female group had higher D, Pd, Mf, Pt, HST, PAG, and DEP figures. 6. As the subjects' age increased, the figure of Hs, D, Pt, Sc, Si, AVD, and SZD increased, while the HST figure decreased. Conclusions : By the above results, we can assume that the sibling relations and the order one is born in the family does not affect one's personality much in grown-ups; whereas the gender and age affect the personality.