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Effect of bicarbonate and progesterone on plasma membrane integrity, acrosome reaction and proportion of fatty acids in boar sperm

  • Park, Choon-Keun;Lee, Sang-Hee
    • Journal of Animal Reproduction and Biotechnology
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    • v.37 no.3
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    • pp.202-208
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    • 2022
  • This study investigated the influence of sodium bicarbonate (NaHCO3) and progesterone on acrosome reaction and proportion of polyunsaturated fatty acid (PUFA) composition boar sperm. The sperm were diluted with semen extender and incubated with NaHCO3 and progesterone at 38℃, 5% CO2 for 6 h. Plasma membrane integrity and acrosome reaction were analyzed using SYBR14/propidium iodide (PI) and FITC-PNA/PI doubling staining method, and proportion of PUFA was analyzed using gas chromatography. In results, Plasma membrane integrity was significantly decreased in 50 mM NaHCO3 group and acrosome reaction was significantly increased by over the 100 mM NaHCO3 group compared to control group (p < 0.05). In addition, progesterone significantly increased decreased plasma membrane integrity at 100 mM progesterone and acrosome reaction at over the 5.0 µM progesterone (p < 0.05), but there was no difference among the 5.0 to 100 µM groups. PUFAs were significantly decreased in 100 mM NaHCO3 and 50 µM progesterone treatments compared to control group. In summary NaHCO3 and progesterone induce acrosome reaction and reduce PUFA composition in boar sperm, therefore, the results maybe help to understand basically knowledge for the acrosome reaction and PUFA composition in boar sperm.

Investigation of Ionospheric Earthquake Precursors Using US-TEC Data during the Solar Maximum of 2013-2015

  • Park, Jeongchan;Park, Sun Mie
    • Journal of Astronomy and Space Sciences
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    • v.37 no.1
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    • pp.61-68
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    • 2020
  • Recent studies have suggested that detectable ionospheric disturbances precede earthquakes. In the present study, variations in the vertical total electron content (TEC) for eight earthquakes with magnitudes of M ≥ 5.5 in the western United States were investigated during the solar maximum of 2013-2015 using United States total electron content (US-TEC) data provided by the National Oceanic and Atmospheric Administration. Analyses of 12 earthquakes with magnitudes of 5.0 ≤ M < 5.5 in the same region were also performed. The TEC variations were examined for 40 days, including the times when the earthquakes occurred. The results indicated a correlation between earthquakes with magnitudes of M ≥ 5.0 and ionospheric TEC anomalies. TEC anomalies occurred before 60% of the earthquakes. Additionally, they were more frequently observed for large earthquakes (75%, M ≥ 5.5) than for small earthquakes (50%, 5.5 > M ≥ 5.0). Anomalous increases in the TEC occurred 2-18 days before the earthquakes as an ionospheric precursor, whereas solar and geomagnetic activities were low or moderate.

Enzymatic Properties of the Membrane-bound NADH Oxidase System in the Aerobic Respiratory Chain of Bacillus cereus

  • Kim, Man-Suk;Kim, Young-Jae
    • BMB Reports
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    • v.37 no.6
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    • pp.753-756
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    • 2004
  • Membranes prepared from Bacillus cereus KCTC 3674, grown aerobically on a complex medium, oxidized NADH exclusively, whereas deamino-NADH was little oxidized. The respiratory chain-linked NADH oxidase exhibited an apparent $K_m$ value of approximately $65\;{\mu}m$ for NADH. The maximum activity of the NADH oxidase was obtained at about pH 8.5 in the presence of 0.1 M KCl (or NaCl). Respiratory chain inhibitor 2-heptyl-4-hydroxyquinoline-N-oxide (HQNO) inhibited the activity of the NADH oxidase by about 90% at a concentration of $40\;{\mu}m$. Interestingly, rotenone and capsaicin inhibited the activity of the NADH oxidase by about 60% at a concentration of $40\;{\mu}m$ and the activity was also highly sensitive to $Ag^+$.

TRAO를 이용한 ORION A의 $^{12}CO$, $^{13}CO$ 관측

  • Kim, Yeong-Sik;Kim, Gwang-Tae
    • The Bulletin of The Korean Astronomical Society
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    • v.37 no.1
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    • pp.78.2-78.2
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    • 2012
  • Orion A 분자운은 별탄생이 활발하게 일어나는 영역이면서 태양계에 비교적 가깝다. 그렇기 때문에 낮은 분해능으로도 자세한 관측이 가능하다. Orion A 분자운까지의 거리가 450 pc 이므로 대덕전파안테나 1' beam으로 0.13 pc 가 된다. 이곳에는 필라멘트 구조가 있는데 FCRAO를 통한 다파장관측을 통해서 필라멘트 구조가 확인되었다.(Melnick et al. 2011). 필라멘트는 길이 4.8 pc, 너비 1.5 pc 로 대덕전파망원경의 1' beam으로 자세한 관측이 가능하였다. 2010년 11월-2011년 5월까지 Orion A 분자운을 대덕전파망원경을 이용하여 $^{12}CO$, $^{13}CO$(J=1-0) 분자선 관측을 하였으며, 관측영역은 적경: 5h 32m - 5h 37m, 적위: $-5^{\circ}$ 14' - $-5^{\circ}$ 37'으로 ($1^{\circ}{\times}1^{\circ}$) 영역을 관측하였다. 그 결과 필라멘트구조를 확인할 수 있었으며 일자 형태로 분포되어 있다는 구조적 결과 얻었다. 관측된 필라멘트 덩어리의 전형적인 크기는 약 0.7 pc, 밀도는 약 $10^4cm^{-3}$, 질량은 약 500 $M_{\odot}$이다. 매우 밀한 곳은 1000 M${\bigodot}$이상의 질량분포도 나타내고 있다. 이것은 이 지역이 일반적인 분자운과 비교했을 때 고밀도 영역임을 나타내고 있다. 더욱 자세한 밀도구조와 질량분포를 밝혀보고 별탄생과의 관련성을 연구하고자 한다.

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Study on Shipboard Fumigation of the Imported Logs (수입원목의 본선훈증소독에 관한 연구)

  • Yu K.Y.;Chung Y.W.;Lee M.H.;Jae J.W.
    • Korean journal of applied entomology
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    • v.23 no.1 s.58
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    • pp.37-41
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    • 1984
  • The $100\%$ of insect pests on the imported logs and Sitophilus oryzae(L.) will be killed both when the imported logs are fumigated on shipboard with 25g methyl $bromide/m^3$ for 24hours at $10\~20^{\circ}C$ and when they are fumigated on shipboard with 37.5g methyl $bromide/m^3$ caculated by DT products for 16 hours at $10\~20^{\circ}C$ using 2 fans of 1800rpm over and ID 30cm. Without using fans, it takes $4\~6$ hours to make gas concentration even in hold. But using 2 fans per hold, the period reduces to $1\~3$ hours.

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A Study on the Marginal Fit of Dental Prosthesis According to the W/P Ratio of Investment (매몰재의 혼수비가 치관 보철물 변연의 적합성에 미치는 영향에 관한 연구)

  • Hwang, Kyung-Sook
    • Journal of Technologic Dentistry
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    • v.18 no.1
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    • pp.67-72
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    • 1996
  • This test is verifying for influence of W/P ratio of investment upon dental prosthesis Firstly we made 40 MOD die and divide 4 groups, each group take 0.2 different w/p ratio grade such as 0.35, 0.37, 0.39 and 0.41. The method of data analysis applied were by ANOVA, Tukey test and Mann-Whiney and Kruskal Wallis test. The finding of this study were as follows : 1. The 0.37 w/p ratio group showed most accurte margin due to $15.8{\mu}m$ gap between margin and die which keep up with manufacture's instruction and 0.35 w/p ratio group was followed as second accuracy group o 새 $48.3{\mu}m$ gap between margin and die, 0.39 w/p ratio group showed $101{\mu}m$ gap and 0.41 w/p ratio group showed $129.8{\mu}m$ gap. 2. As to the relationship of the margin accuracy between 4 different grade of w/p ratio groups, each group was statistically significant(P<0.01). Also the all groups were statistically significant except between 0.35 and 0.37 groups between 0.39 and 0.41 groups, and between 0.35 and 0.39 groups. 3. A consequence of Mann-Whitney & Kruskal-Wallis test for marginal accuracy between 4 different grade of w/p ratio was statistically significant as same as above ANOVA test result. Also Tukey test for verifying similarity from each group showed same as above.

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A Comparison of the Differences, Relationships and Impacts between the Self-Concept and Career Development for Elementary Schoolers by gender (초등학생의 성에 따른 자아개념과 진로발달 간 차이, 관계 및 영향력 비교)

  • Kim, Ji Young;Suh, Young Sook
    • Korean Journal of Childcare and Education
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    • v.7 no.1
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    • pp.1-22
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    • 2011
  • The purpose of this study was a comparative of the differences, relationships and impacts between the self-concept and career development for elementary schoolers by gender. The subjects of this study were 259(133 boys and 126 girls) elementary schoolers. The instrument used in this study was Jung Jong Jin's(1996) self-concept Inventory and Ahn Chang gyu & Ahn Hyeon eui's(2006) Holland career development Inventory. The collected data were analyzed with a SPSS 12.0 program. The major findings of the study were as follows: (1) the mean score of boy(M=54.857, SD=7.593) was higher than girl(M=52.302, SD=8.908) in 'emotional-self'. (2) (1) the mean score of girl(M=42.200, SD=5.918) was higher than boy(M=37.372, SD=7.600) in 'gender-related prejudice about occupation'. (2) the mean score of boy(M=36.544, SD=5.544) was higher than girl(M=35.008, SD=5.118) in 'autonomy' (3) the revealed that self-concept was highly correlated with boy's(r=.175~.761) and girl's(r=.199~.667) career development. (4) (1) the 'academic-self' had an impact of the boy's(5.6%~56.3%) and girl's(8.4%~48.7%) career development. (2) the 'social-self' had an impact of the boy's(2.6%~5.9%) and girl's(2.7%~4.3%) career development. (3) the 'emotional-self' had an impact of the boy's(1.9%~37.0%) and girl's(11.2%) career development. (4) the 'physical-self' no had an impact of the boy's and girl's career development. The findings of the study illustrated that the self-concept for elementary schoolers had an impact career development.

Wavefront Compensation Using a Silicon Carbide Deformable Mirror with 37 Actuators for Adaptive Optics (적응광학계용 37채널 SiC 변형거울을 이용한 파면 보상)

  • Ahn, Kyohoon;Rhee, Hyug-Gyo;Lee, Ho-Jae;Lee, Jun-Ho;Yang, Ho-Soon;Kihm, Hagyong
    • Korean Journal of Optics and Photonics
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    • v.27 no.3
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    • pp.106-113
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    • 2016
  • In this paper, we deal with the wavefront compensation capability of a silicon carbide (SiC) deformable mirror (DM) with 37 actuators for adaptive optics. The wavefront compensation capability of the SiC DM is predicted by computer simulation and examined by actual experiments with a closed-loop adaptive optics system consistsing of a light source, a phase plate, a SiC DM, a high speed Shack-Hartmann sensor, and a control computer. Distortion of wavefront is caused by the phase plate in the closed-loop adaptive optics system. The distorted wavefront has a peak-to-valley (PV) wavefront error of $0.3{\mu}m{\sim}0.9{\mu}m$ and root-mean-square (RMS) error of $0.06{\mu}m{\sim}0.25{\mu}m$. The high-speed Shack-Hartmann sensor measures the wavefront error of the distortion caused by the phase plate, and the SiC DM compensates for the distorted wavefront. The compensated wavefront has residual errors lower than $0.1{\mu}m$ PV and $0.03{\mu}m$ RMS. Consequently, we conclude that we can compensate for the distorted wavefront using the SiC DM in the closed-loop adaptive optics system with an operating frequency speed of 500 Hz.

Simultaneous diagnosis and differentiation of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis infections by multiplex PCR (Mycoplasma hyopneumoniae와 Mycoplasma hyorhinis 동시 감별진단을 위한 다중진단 중합효소반응)

  • Hong, Sunhwa;Lee, Hyun-A;Kim, Dong-Woo;Kim, Tae-Wan;Kim, Okjin
    • Korean Journal of Veterinary Service
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    • v.37 no.4
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    • pp.247-252
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    • 2014
  • The economic impact of swine mycoplasma infection is high. An accurate diagnosis is often difficult and time consuming. We report the development and validation of an effective multiplex polymerase chain reaction (PCR) assay that detects Mycoplasma (M.) hyopneumoniae and M. hyorhinis. The multi detection of M. hyopneumoniae and M. hyorhinis primer set were employed to detect mycoplasma species and typing of the species was performed on the basis of sequence analysis of the PCR product. The target nucleic acid fragments were specifically amplified by M. hyopneumoniae and M. hyorhinis PCR with 16S ribosomal DNA primers. Single and mixed Mycoplasma species DNA templates were used to evaluate the specificity of the multiplex assay. The corresponding specific DNA products were amplified for each pathogen. The multiplex PCR assay provides a novel tool for simultaneous detection and differentiation of M. hyopneumoniae and M. hyorhinis.

A Comparative Analysis for the Performance of 200 N-class Gaseous Methane-Liquid Oxygen Small Rocket Engine According to the Characteristic Length Variation (특성길이 변화에 따른 200 N급 기체메탄-액체산소 소형로켓엔진의 성능 비교 분석)

  • Kang, Yun Hyeong;Ahn, Hyun Jong;Kim, Jeong Soo
    • Journal of the Korean Society of Propulsion Engineers
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    • v.24 no.6
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    • pp.85-92
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    • 2020
  • Ground hot-firing tests were conducted to analyze the combustion performance according to the characteristic lengths 1.37 m, 1.71 m, and 2.06 m of the combustion chamber in 200 N-class GCH4-LOx small rocket engine. Thrust, specific impulse, and characteristic velocity at the steady-state could be obtained as the key performance parameters of the rocket engine. The performance characteristics acquired through the test were compared and analyzed with the theoretical performance calculated from CEA analysis. Observation of the influence of characteristic length on the combustion performance indicates that an optimal characteristic length shall remain between 1.71 m and 2.06 m.