• Journal of Embryo Transfer
• /
• v.28 no.3
• /
• pp.273-280
• /
• 2013

The aim of this study was to establish a three dimensional (3D) culture system of endometrial cells and to examine the plasminogen activators (PAs) activity in porcine uterine. The 3D culture system in porcine endometrial cells was composed to mixture 3D gel, stromal cells and epithelial cells. The 3D culture system was used to identify normal structure search as uterine tissue and PAs expression in this study. In results, porcine endometrium epithelial cells forming a top monolayer and endometrium stromal cells developed as fibroblast-like within 3D matrix scaffold. Expression of urokinase-type PA (uPA) and tissue-type PA (tPA) were observed during the 3D culture using immunofluorescence. PA activity in 3D-cultured endometrial cells was no significant difference between the tissue type, but 2D culture system were significantly lower than in 3D-cultured endometrial cells (P<0.05). Therefore, basic system and functional aspect of 3D culture could be established with similar system of endometrium tissue. We suggest that this study was assumed applicable as baseline data to investigate mechanism between porcine uterus cells in vitro.

• Journal of Technologic Dentistry
• /
• v.44 no.2
• /
• pp.31-37
• /
• 2022

Purpose: The aim of this study was to evaluate the accuracy of provisional crowns manufactured using a milling machine and a digital light processing (DLP) printer. Methods: A full-contour crown was designed using computer-aided design software. Provisional crowns of this design were manufactured using a milling machine and using a DLP three-dimensional (3D) printer (N=20). The provisional crowns were digitized with an extraoral scanner, and 3D deviation analysis was applied to the scanned data to confirm their accuracy. An independent t-test was performed to detect the significant differences, and the Kolmogorov-Smirnov test was used for analysis (α=0.05). Results: No significant differences were found among the precision of marginal surface between the printed and milled crowns (p=0.181). The trueness of marginal and internal surfaces of the milled crowns were statistically higher than those of the printed crowns (p=0.024, p=0.001; respectively). Conclusion: The accuracy of provisional crowns manufactured using a milling machine and a 3D printer differed significantly except with regards to the precision of the internal surface. However, all the crowns were clinically acceptable, regardless of the manufacturing method used.

• Journal of Korean Ophthalmic Optics Society
• /
• v.17 no.2
• /
• pp.165-170
• /
• 2012

Purpose: The aim of this study was to measure and evaluate changes of visual responses in viewing a 2D and 3D (three-dimensional) image. Methods: The subjects were 44 college students aged 19 to 25 years with normal binocular vision. The visual responses measured were CA/C (convergence accommodation/convergence) ratio, convergence-induced PD(interpupillary distance), accommodative responses, perceived distance in viewing a 3D image. Results: Convergence and accommodative responses in viewing the 3D image were significantly larger (p<0.05) than in 2D. A moderate positive correction was found between CA/C ratio and accommodative response (r = 0.477, p = 0.001). It was indicated that smaller PD had larger depth perception. Convergence in viewing the 3D image was significantly larger (p<0.05) than that at cognitive distance. Conclusions: The visual fatigue may be more intense in larger CA/C ratio and smaller PD when viewing 3D images.

• The Journal of Korean Academy of Prosthodontics
• /
• v.36 no.3
• /
• pp.496-505
• /
• 1998

The purpose of this study was to evalute the effect of immersion disinfection on the dimensional stability of rubber impression materials. The metallic master model was made in order to simulate the intraoral arch form. Impressions were made from four impression materials (Exaflex, Extrude, Reprosil, Impregum-F) and immersed in three disinfectant solutions (Wydex, Vi-Pon, Potadine). Casts from the impressions were measured according to the interpreparation distance. The A-B and The B-D abutment distance were compared with the control group and disinfected groups. The results were as follows; 1. The measurements of the stone cast increased relative to the master model and there was a significant difference (P<0.05). 2. The relative dimensional change of the stone cast as compared with master model ranged from 0.10% to 0.56% in the A-B distance and ranged from 0.04% to 0.27% in the B-D distance. 3. The dimensional change of the disinfected groups as compared with the control group was significantly different in the three impression materials except for Impregum-F (P<0.05). 4. The relative dimensional change of the disinfected groups compared with the control group ranged from 0% to 0.20% in the A-B distance and ranged from -0.09% to 0.11% in the B-D distance. These results suggest that immersion disinfection of rubber impression materials by chemical disinfectants causes very small dimensional change and the change is clinically acceptable for prothesis fabrication

• The Journal of Advanced Prosthodontics
• /
• v.11 no.3
• /
• pp.179-186
• /
• 2019

PURPOSE. The purpose of this study was to compare two novel impression methods and a conventional impression method for edentulous jaws using 3-dimensional (3D) analysis software. MATERIALS AND METHODS. Five edentulous patients (four men and one woman; mean age: 62.7 years) were included. Three impression techniques were used: conventional impression method (CI; control), simple modified closed-mouth impression method with a novel tray (SI), and digital impression method using an intraoral scanner (DI). Subsequently, a gypsum model was made, scanned, and superimposed using 3D analysis software. Mean area displacement was measured using CI method to evaluate differences in the impression surfaces as compared to those values obtained using SI and DI methods. The values were confirmed at two to five areas to determine the differences. CI and SI were compared at all areas, while CI and DI were compared at the supporting areas. Kruskal-Wallis test was performed for all data. Statistical significance was considered at P value <.05. RESULTS. In the comparison of the CI and SI methods, the greatest difference was observed in the mandibular vestibule without statistical significance (P>.05); the difference was < 0.14 mm in the maxilla. The difference in the edentulous supporting areas between the CI and DI methods was not significant (P>.05). CONCLUSION. The CI, SI, and DI methods were effective in making impressions of the supporting areas in edentulous patients. The SI method showed clinically applicability.

• Korean Journal of Radiology
• /
• v.22 no.3
• /
• pp.435-441
• /
• 2021

Objective: To evaluate the usefulness of the ventricular volume percentage quantified using three-dimensional (3D) brain computed tomography (CT) data for interpreting serial changes in hydrocephalus. Materials and Methods: Intracranial and ventricular volumes were quantified using the semiautomatic 3D threshold-based segmentation approach for 113 brain CT examinations (age at brain CT examination ≤ 18 years) in 38 patients with hydrocephalus. Changes in ventricular volume percentage were calculated using 75 serial brain CT pairs (time interval 173.6 ± 234.9 days) and compared with the conventional assessment of changes in hydrocephalus (increased, unchanged, or decreased). A cut-off value for the diagnosis of no change in hydrocephalus was calculated using receiver operating characteristic curve analysis. The reproducibility of the volumetric measurements was assessed using the intraclass correlation coefficient on a subset of 20 brain CT examinations. Results: Mean intracranial volume, ventricular volume, and ventricular volume percentage were 1284.6 ± 297.1 cm³, 249.0 ± 150.8 cm³, and 19.9 ± 12.8%, respectively. The volumetric measurements were highly reproducible (intraclass correlation coefficient = 1.0). Serial changes (0.8 ± 0.6%) in ventricular volume percentage in the unchanged group (n = 28) were significantly smaller than those in the increased and decreased groups (6.8 ± 4.3% and 5.6 ± 4.2%, respectively; p = 0.001 and p < 0.001, respectively; n = 11 and n = 36, respectively). The ventricular volume percentage was an excellent parameter for evaluating the degree of hydrocephalus (area under the receiver operating characteristic curve = 0.975; 95% confidence interval, 0.948-1.000; p < 0.001). With a cut-off value of 2.4%, the diagnosis of unchanged hydrocephalus could be made with 83.0% sensitivity and 100.0% specificity. Conclusion: The ventricular volume percentage quantified using 3D brain CT data is useful for interpreting serial changes in hydrocephalus.

• Journal of Technologic Dentistry
• /
• v.38 no.3
• /
• pp.185-191
• /
• 2016

Purpose: The purpose of this study were to evaluate the quality of dental prostheses printed by 3-dimensional printing system. Methods: Mater model was prepared and ten study models were fabricated. Ten single crowns were printed by 3D-printing system(Resin group) and another ten single crowns using casting method were manufactured(Metal group). The marginal adaptation of single crowns were measured using by silicone replica technique. Silicone replicas were sectioned four times. The marginal adaptations were evaluated using by digital microscope. Statistical analyses were performed with Mann-Whitney test(${\alpha}=0.05$). Results: $Mean{\pm}standard$ deviations of all marginal adaptations were $92.1(20.0){\mu}m$ for Metal group and $69.7(12.3){\mu}m$ for Resin group. Two groups were no statistically significant differences(p>0.05). Conclusion: Marginal adaptation of single crowns printed by 3D-printing system were ranged within the clinical recommendation.

• Imaging Science in Dentistry
• /
• v.48 no.2
• /
• pp.111-119
• /
• 2018

Purpose: This study was conducted to evaluate the accuracy of linear measurements of 3-dimensional (3D) images generated by cone-beam computed tomography (CBCT) and facial scanning systems, and to assess the effect of scanning parameters, such as CBCT exposure settings, on image quality. Materials and Methods: CBCT and facial scanning images of an anthropomorphic phantom showing 13 soft-tissue anatomical landmarks were used in the study. The distances between the anatomical landmarks on the phantom were measured to obtain a reference for evaluating the accuracy of the 3D facial soft-tissue images. The distances between the 3D image landmarks were measured using a 3D distance measurement tool. The effect of scanning parameters on CBCT image quality was evaluated by visually comparing images acquired under different exposure conditions, but at a constant threshold. Results: Comparison of the repeated direct phantom and image-based measurements revealed good reproducibility. There were no significant differences between the direct phantom and image-based measurements of the CBCT surface volume-rendered images. Five of the 15 measurements of the 3D facial scans were found to be significantly different from their corresponding direct phantom measurements(P<.05). The quality of the CBCT surface volume-rendered images acquired at a constant threshold varied across different exposure conditions. Conclusion: These results proved that existing 3D imaging techniques were satisfactorily accurate for clinical applications, and that optimizing the variables that affected image quality, such as the exposure parameters, was critical for image acquisition.

• Proceedings of the Korean Vacuum Society Conference
• /
• 2016.02a
• /
• pp.291.2-291.2
• /
• 2016

3D semiconductor material of silicon that is used throughout the semiconductor industry currently faces a physical limitation of the development of semiconductor process technology. The research into the next generation of nano-semiconductor materials such as semiconductor properties superior to replace silicon in order to overcome the physical limitations, such as the 2-dimensional graphene material in 2D transition-metal dichalcogenide (TMD) has been researched. In particular, 2D TMD doping without severely damage of crystal structure is required different conventional methods such as ion implantation in 3D semiconductor device. Here, we study a p-type doping technique on tungsten diselenide (WSe2) for p-channel 2D transistors by adjusting the concentration of hydrochloric acid through Raman spectroscopy and electrical/optical measurements. Where the performance parameters of WSe2 - based electronic device can be properly designed or optimized. (on currents increasing and threshold voltage positive shift.) We expect that our p-doping method will make it possible to successfully integrate future layered semiconductor devices.

• Korean Chemical Engineering Research
• /
• v.55 no.5
• /
• pp.698-703
• /
• 2017

Bubble flow characteristics in fluidized beds were analyzed by CPFD simulation. A fluidized bed, which had the size of $0.3m-ID{\times}2.4m-high$, was modeled by commercial CPFD $Barracuda^{(R)}$. Properties of bed material were $d_p=150{\mu}m$, ${\rho}_p=2,330kg/m^3$, and $U_{mf}=0.02m/s$. Gas was uniformly distributed and the range of superficial gas velocity was 0.07 to 0.16 m/s. Two other geometries were modeled. The first was a three-dimensional model, and the other was a two-dimensional model of $0.01m{\times}0.3m{\times}2.4m$. Bubble size and rising velocity were simulated by axial and radial position according to superficial gas velocity. In the case of three-dimensional model, simulated bubble rising velocity was different from correlations, because there was zigzag motion in bubble flow, and bubble detection was duplicated. To exclude zigzag motion of bubble flow, bubble rising velocity was simulated in the two-dimensional model and compared to the result from three-dimensional model.