• Design & Manufacturing
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• v.15 no.2
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• pp.11-16
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• 2021

Recently, three-dimensional (3D) cell culture systems, which are superior to conventional two-dimensional (2D) vascular systems that mimic the in vivo environment, are being actively studied to reproduce drug responses and cell differentiation in organisms. Conventional two-dimensional cell culture methods (scaffold-based and non-scaffold-based) have a limited cell growth rate because the culture cannot supply the culture medium as consistently as microvessels. To solve this problem, we would like to propose a 3D culture system with an environment similar to living cells by continuously supplying the culture medium to the bottom of the 3D cell support. The 3D culture system is a structure in which microvascular structures are combined under a scaffold (agar, collagen, etc.) where cells can settle and grow. First, we have manufactured molds for the formation of four types of microvessel-mimicking chips: width / height ①100 ㎛ / 100 ㎛, ②100 ㎛ / 50 ㎛, ③ 150 ㎛ / 100 ㎛, and ④ 200 ㎛ / 100 ㎛. By injection molding, four types of microfluidic chips were made with GPPS (general purpose polystyrene), and a 100㎛-thick PDMS (polydimethylsiloxane) film was attached to the top of each microfluidic chip. As a result of observing the flow of the culture medium in the microchannel, it was confirmed that when the aspect ratio (height/width) of the microchannel is 1.5 or more, the fluid flows from the inlet to the outlet without a backflow phenomenon. In addition, the culture efficiency experiments of colorectal cancer cells (SW490) were performed in a 3D culture system in which PDMS films with different pore diameters (1/25/45 ㎛) were combined on a microfluidic chip. As a result, it was found that the cell growth rate increased up to 1.3 times and the cell death rate decreased by 71% as a result of the 3D culture system having a hole membrane with a diameter of 10 ㎛ or more compared to the conventional commercial. Based on the results of this study, it is possible to expand and build various 3D cell culture systems that can maximize cell culture efficiency by cell type by adjusting the shape of the microchannel, the size of the film hole, and the flow rate of the inlet.

• Proceedings of the IEEK Conference
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• 2005.11a
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• pp.797-800
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• 2005

In this paper, the designed 10-bit current steering data driving circuit consists of bias circuits, shift registers, data and line latches, level shifters, and 10-bit D/A converters. This data driving circuit can improve image quality, driving speed, and can reduce process error, DNL error, and glitch noise. To reduce current cells, the 10-bit D/A converter was designed 3+3+4 hybrid type. As a result 49 current cells are decreased. The transient analysis shows that currents flows a few of mA in data line and the currents have 1024 gray levels of current values. Total circuits are designed for 10 ${\mu}s$ speed. Thus the designed 10-bit current steering data driving circuit can be usable in HDTV/XGA AMOLED displays. These data driving circuits are designed for 0.35 ${\mu}m$ CMOS process at 3.3 V and 18 V supply voltage and simulated with HSPICE..

• Journal of the Society of Naval Architects of Korea
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• v.50 no.3
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• pp.160-166
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• 2013

Recently submerged objects moving at high speed such as a supercavitating torpedo have been studied for their practical advantage of the dramatic drag reduction. In this study, we are focusing our attention on supercavitating flows around axisymmetric cavitators; a numerical method based on inviscid flow is developed and predicted supercavities around several shapes of 2D and 3D cavitators are presented. The results are validated by comparison of existing theoretical and empirical results. In addition, characteristic features of supercavity shapes and drag forces acting on a real scale torpedo are evaluated according to practically appropriate operating conditions.

• Journal of Environmental Health Sciences
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• v.34 no.4
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• pp.285-291
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• 2008

This study was performed to investigate the effects of Chitosan on the nickel poisoning in rats. In the study, 150 male Sprague-Dawley were used. The experimental groups were divided into four: A (30 mg/L nickel), B (30 mg/L nickel+0.2% Chitin, Chitosan and Dithiocarbamate Chitosan), C (30 mg/L nickel+0.4% Chitin, Chitosan and Dithiocarbamate Chitosan), D (30 mg/L nickel+0.8%Chitin, Chitosan and Dithiocarbamate Chitosan). The results were as flows; 1. The nickel concentration in the livers of the control group (A) was $0.153{\sim}0.186\;mg/kg$ but the nickel concentration in the livers of the experimental decreased during the experimental period (P<0.05). 2. Metallothionenin levels in rat liver were $2.77{\sim}3.25\;ug/g$ wet,wt in control group (A), but were $2.89{\sim}3.51\;ug/g$ wet,wt (B), $2.97{\sim}3.62\;ug/g$ wet,wt (C), $2.68{\sim}3.68\;ug/g$ wet,wt (D). Respectively in the experimental groups. The experimental groups were inclined to increase compare to the control group (P<0.05). In conclusion, this study revealed a preventive effect of Chitin, Chitosan and Dithiocarbamate Chitosan against nickel toxicity.

• 한국가시화정보학회:학술대회논문집
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• 2006.12a
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• pp.1-6
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• 2006

Single-Camera Stereoscopic Vision three-dimensional measurement system has been developed based upon 30-PTV algorithm. The system consists of one camera $(1k\times1k)$ and a host computer. To attain three-dimensional measurements a plate having stereo holes has been installed inside of the lens system. Three-dimensional measurements was successfully attained by adopting the conventional 30-PTV camera calibration methods. As applications of the constructed measurement system, a water droplet mixed with alcohol was constructed on a transparent plastic plate with the contacted fluid diameter 4mm, and the particles motions inside of the droplet have been investigated with the constructed measurement system. The measurement uncertainty of the constructed system was 0.04mm, 0.04mm and 0.09mm for X, Y and Z coordinates.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.8
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• pp.1124-1130
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• 2014

The objective of this study was to determine the responses in the digestibility of dry matter (DM) and amino acid (AA) composition of ileal endogenous flow (IEF) of pigs (initial body weight, $69.1{\pm}6.46$ kg) fed N-free diets (NFD) formulated with different ratios of corn starch to dextrose. Fifteen pigs fitted with a T-cannula at the distal ileum were fed 5 diets according to a triplicated $5{\times}2$ incomplete Latin-square design. Each period consisted of a 5-d adjustment period and 2 d of ileal digesta collection for 12 h on each of d 6 and 7 and between each period, there was a 5-d recovery period to avoid abnormal weight loss. The ratios of corn starch to dextrose investigated were 0:879, 293:586, 586:293, 779:100, and 879:0 for diet numbers 1, 2, 3, 4 and 5, respectively, and chromic oxide (5 g/kg) was used as an indigestible index. Ileal DM digestibility was greater in Diet 1 than that in Diet 4 (89.5% vs 87.3%, p<0.01) but they were not different from Diet 2, 3, or 5. The IEF for most of indispensable AA were not different among diets with the exception of Met, in which a lack of corn starch or dextrose gave lower (p = 0.028) IEF of Met than diets containing corn starch and dextrose. Likewise, the dispensable AA and total AA in the IEF did not differ among diets. The respective IEF of AA (mg/kg of dry matter intake) in pigs fed Diets 1, 2, 3, 4, or 5 were 301, 434, 377, 477,or 365 for Lys, 61, 89, 71, 87, or 61 for Met, and 477, 590, 472, 520, or 436 for Thr. Proline was the most abundant AA in the IEF followed by Gly, Glu, and Asp and together accounted for approximately 50% of the total ileal AA flows of pigs fed NFD. In conclusion, the variation in proportion of corn starch and dextrose in a NFD does not largely affect estimates of IEF of N and AA for growing-finishing pigs.

• Korean Journal of Agricultural Science
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• v.37 no.3
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• pp.481-490
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• 2010

Baekgog reservoir is located in Jincheon county, Chungbuk province, of which full water levels will be heightened from EL. 100.1 m to EL. 102.1 m, and total storages from 21.75 $Mm^3$ to 26.67 $Mm^3$. The simulation for reservoir inflow was conducted by DAWAST model. The annual average irrigation water was estimated to 33.19 $Mm^3$ supplied to 2,975 ha and the instream flows could be allocated with 0.14 mm/d from October to April with annual average of 2.52 $Mm^3$. The operation rule curve was drawn using inflow, irrigation, and instream flow requirements data. The reservoir water storage was simulated on a daily basis in case of both normal and withdrawal limit operation. In case of normal operation, the annual average irrigation water supply increased from 31.95 $Mm^3$ to 33.32 $Mm^3$, the instream water supply from 2.40 $Mm^3$ to 2.44 $Mm^3$, the water storages from 15.74 $Mm^3$ to 19.88 $Mm^3$, and the water supply reliability from 77.3 % to 81.6 %. In case of operation with withdrawal limit, the amount of instream water supply was 2.52 $Mm^3$ from reservoir regardless of the condition while the water storage increased from 16.77 $Mm^3$ to 20.65 $Mm^3$. The irrigation water supply capacity was appropriate for the case of normal operation with 2 m heightened condition. The present instream water supply capacity was 35,000 $m^3$/d (6.86 $Mm^3$/y) while 42,000 $m^3$/d (8.36 $Mm^3$/y) in 2 m heightened condition in case of withdrawal limit operation.

• Journal of Magnetics
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• v.15 no.3
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• pp.128-131
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• 2010

In this paper, we propose a new design of the permanent magnet reluctance wheel which will make it possible to attach the robot to a vertical plane and move it. In the newly suggested design, a permanent magnet is utilized to enhance the adhesive force during attachment, and an electromagnet is produced to weaken the magnetic field of the permanent magnet and reduce the adhesive force for easier detachment of wheels from steel plates. To characterize the performance of this new wheel design, a 3-D finite element analysis is executed using a commercial FE program. The results show that the adhesive force is reduced effectively by the electromagnet which flows in the reverse direction of the magnetic loop of the permanent magnet when the current is supplied to the coil.

• Proceedings of the KSME Conference
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• 2002.08a
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• pp.189-192
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• 2002

In this paper, the submarine model, called DARPA SUBOFF model, has been numerically analyzed to investigate the aerodynamic forces variation in terms of angle of attacks and yaw angles. The SUBOFF model is consisted of the three parts : axisymmetric body, fairwater, and four symmetric stern appendages. Three dimensional unsteady incompressible Wavier-Stokes equation was used on curvilinear multi-block grid system. To validate the present code, the SUBOFF tare hull and an ellipsoid at angle of attacks of $10^{\circ}\;and\;30^{\circ}$ were simulated and a good agreement with experiments was obtained. After the code validation, the flows over SUBOFF model were simulated at three different angle of attacks and yaw angles. The variation of aerodynamic forces in terms of angle of attack and yaw angle were calculated. Also, to understand the flow features around a submarine with variation of yaw and attack angle, the pressure contours and streamlines were plotted.

• Journal of computational fluids engineering
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• v.3 no.2
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• pp.17-26
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• 1998

The three-dimensional incompressible Navier-Stokes equations have been solved by a node-centered finite volume method with unstructured hybrid grids. The pressure-velocity coupling is handled by the artificial compressibility algorithm and convective fluxes are obtained by Roe's flux difference splitting scheme with linear reconstruction of the solutions. Euler implicit method with Jacobi matrix solver is used for the time-integration. The viscous terms are discretised in a manner to handle any kind of grids such as tetragedra, prisms, pyramids, hexahedra, or mixed-element grid. Inviscid bump flow is solved to check the accuracy of high order convective flux discretisation. And viscous flows around a circular cylinder and a sphere are studied to show the efficiency and accuracy of the solver.