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Application of the CRISPR/Cas System for Point-of-care Diagnosis of Cattle Disease (현장에서 가축질병을 진단하기 위한 CRISPR/Cas 시스템의 활용)

  • Lee, Wonhee;Lee, Yoonseok
    • Journal of Life Science
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    • v.30 no.3
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    • pp.313-319
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    • 2020
  • Recently, cattle epidemic diseases are caused by a pathogen such as a virus or bacterium. Such diseases can spread through various pathways, such as feed intake, respiration, and contact between livestock. Diagnosis based on the ELISA (Enzyme-linked immunosorbent assay) and PCR (Polymerase chain reaction) methods has limitations because these traditional diagnostic methods are time consuming assays that require multiple steps and dedicated equipment. In this review, we propose the use of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) Cas system based on DNA and RNA levels for early point-of-care diagnosis in cattle. In the CRISPR/Cas system, Cas effectors are classified into two classes and six subtypes. The Cas effectors included in class 2 are typically Cas9 in type II, Cas12 in type V (Cas12a and Cas12b) and Cas13 in type VI (Cas13a and Cas13b). The CRISPR/Cas system uses reporter molecules that are attached to the ssDNA strands. When the Cas enzyme cuts the ssDNA, these reporters either fluoresce or change color, indicating the presence of a specific disease marker. There are several steps in the development of a CRISPR/Cas system. The first is to select the Cas enzyme depending on DNA or RNA from pathogens (viruses or bacteria). Based on that, the next step is to integrate the optimal amplification, transducing method, and signal reporter. The CRISPR/Cas system is a powerful diagnostic tool using a gene-editing method, which is faster, better, and cheaper than traditional methods. This system could be used for early diagnosis of epidemic cattle diseases and help to control their spread.

Development of the Balance Chair for Improving Postural Control Ability & Pelvic Correction (골반교정 및 자세균형능력 증진을 위한 균형의자 개발)

  • Oh, Seung-Yong;Shin, Sun-Hye;Kang, Seung-Rok;Hong, Chul-Un;Kwon, Tae-Kyu
    • Journal of rehabilitation welfare engineering & assistive technology
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    • v.11 no.3
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    • pp.271-277
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    • 2017
  • The purpose of this study was to develop a balance chair for improving pelvic correction and postural balance through postural balance training using tactile feedback by a vibration motor provided in real time according to the user's attitude. We built a body frame using mono cast(MC) Nylon, Touch thin film transistor(TFT) for user interface, a main control module using Arduino, a 9-axis acceleration sensor for user's posture determination, and a vibration module for tactile feedback. The prototype of the Balance Chair which surrounds the outside was made with cushion for sitting conformability. In order to verify the effectiveness of the postural balance training system using the built prototype, the muscle activity (% MVIC) of the left and right iliocostalis lumborum those are the main muscles of the spinal movement was measured with ten female subjects. And the balance ability before and after training was measured using Spine Balance 3D, a posture balance ability evaluation device. The muscular activities of the left and right iliocostalis lumborum showed the balance activation according to vibration feedback during exercise protocol and postural balance improved after balance exercise training using balance chair. This study could be apply to use the fundamental research for developing the various postural balance product.

Analysis of advancement model of 1st generation dairy smart farm based on Open API application (개방형 제어기반 1세대 낙농 스마트팜의 고도화 모델 적용 분석)

  • Yang, Kayoung;Kwon, Kyeong-Seok;Kim, Jung Kon;Kim, Jong Bok;Jang, Dong Hwa;Ko, miae
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.11
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    • pp.180-186
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    • 2020
  • ICT convergence using smart livestock is that in the first-generation dairy smart farm model, each device made by several manufacturers uses its own communication method, limiting the mutual operation of each device. This study uses a model based on open control technology to secure interoperability of existing ICT devices and to manage data efficiently. The open integrated control derived from this process is the software interface structure of Open API. It is an observer that serves as real-time data collection according to the communication method of ICT devices and sensors located at each end. It consists of a broker that connects and transmits to the upper integrated management server. As a result of the performance analysis through verification of two first-generation dairy smart farm model sites, the average daily milk production increased compared to the previous year (farm A 5.13%, farm B 1.33%, p<0.05). Cow days open (DO) was reduced by 17.5% on farm A and 13.3% for farm B(p<0.05). Cows require an adaptation period after the introduction of the ICT device, but if continuous effects are observed, the effect of production can be expected to increase gradually.

Development of Marking Robot by using Arc Welding for Shipbuilding (조선 적용을 위한 문자마킹 자동용접장치 개발)

  • Park, Chul-Sung;Park, Jin-Whi;Ryu, Young-Soo;Lee, Jeong-Soo
    • Proceedings of the KWS Conference
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    • 2009.11a
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    • pp.3-3
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    • 2009
  • 선박의 건조과정에서 필수적으로 선체 외판에는 선박의 안전과 운항 및 정비 등에 필요한 정보를 나타내기 위해 다양한 종류의 마크 및 문자가 마킹되어진다. 하지만, 단순한 도장 작업만으로는 해상과 같은 부식 환경에서 마크 및 문자가 쉽게 지워지거나 손상되기 때문에 마크 및 문자를 용접 비드(welding bead)로 표시하거나 미리 절단된 강판(steel plate)을 수동으로 용접한 뒤 도장을 함으로써 마크 및 문자의 손상을 방지하고 있다. 이러한 문자마킹작업을 하기 위해서는 작업자가 수작업으로 기준선과 마크 및 문자의 위치를 먹줄 등을 이용하여 마킹을 하고, 해당 마크 및 문자의 템플렛(template)을 이용하여 펀칭을 실시한 후 수동으로 용접을 실시한다. 하지만, 수작업을 통한 선체외판 문자마킹 작업은 작업자의 기량에 따라 품질이 상이하여 품질 저하의 원인이 된다. 또한 대조립 및 탑재 단계에서 문자 마킹 작업시 수직자세의 용접을 요구함으로써 작업자가 안전사고에 노출되어 있으며, 선박의 각 단계별 주요 공정보다 작업시간이 길어져 전체 선박 건조공정을 지연시키는 문제점 등을 야기시킬 수 있다. 이러한 문제점들을 해결하기 위해 조선업계에서는 선체 외판의 마크 및 문자를 자동으로 용접할 수 있는 장치를 개발하기 위해 노력해왔으며, 몇몇 개발 사례가 보고되고 있다. 하지만, 그 실효성 부분에서는 아직까지 해결하지 못한 문제점들로 인해 현장 적용에는 어려움을 보이고 있다. 본 연구에서는 선박외판 문자 자동용접장치의 기능성뿐만 아니라 현업 적용성을 가장 우선적으로 고려하여 문자마킹장치(Marking Robot for Shipbuilding) 개발을 진행하였다. 우선, 적절한 용접 재료를 선정하기 위해서 솔리드 와이어(Solid Wire)와 플럭스 코어드 와이어(Flux Cored Wire)에 대한 비드온 용접(Bead-On Welding)을 아래보기자세와 수직자세에 대해서 실시하여 적절한 용접 조건을 설정하였다. 본 연구에서 개발된 문자마킹 자동용접장치는 3축으로 구성되어 있으며 각 축들을 분리할 수 있도록 개발하여 이동성을 향상시켰으며, 작업면과 용접토치간의 거리를 일정하게 유지시킬 수 있도록 용접전류 센서(Welding Current Sensor)를 이용하여 토치 높이(Wire Extension)를 제어함으로써 균일한 품질의 용접비드를 얻을 수 있었다. 또한 문자마킹 자동용접장치는 본체 구동부와 제어부(Touch Screen)가 쉽게 분리되고 장착이 가능한 구조로 개발되었으며, 용접시 각 용접자세별로 용접전압, 전류 그리고 용접속도 설정이 가능하여 아래보기 자세뿐만 아니라 어떠한 자세에서도 같은 모양의 비드형상을 가지는 문자마킹용접이 가능하도록 개발하였으며, 이는 실험과 현장적용을 통해 검증하였다.

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Numerical Simulation of Three Dimensional Fluid Flow Phenomena in Cylindrical Submerged Flat Membrane Bioreactor for Aeration Rate (원통 침지형 평막 생물반응기 내 산기량에 따른 3차원 유동현상에 관한 수치모사)

  • Kim, Dae Chun;Chung, Kun Yong
    • Applied Chemistry for Engineering
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    • v.25 no.4
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    • pp.401-408
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    • 2014
  • In membrane bio-reactor (MBR), the aeration control is one of the important independent variables to decrease fouling and to save energy with shear stress change on the membrane surface. The paper was carried out for numerical simulation of 3-dimensional fluid flow phenomena of the cylindrical bioreactor with submerged flat membranes equipped in the center and supplied the air from the bottom by using the COMSOL program. The viscosity and temperature of solution were assumed to be constant, and the specific air demand based on permeate volume ($SAD_p$) defined as scouring air per permeate rates was used as a variable. The calculated CFD velocities were compared with those of the velocity meter measurement and video image analysis, respectively. The results were good agreement each other within 11% error. For fluid flow in the reactor the liquid velocity increased rapidly between the air diffuser and membrane module, but the velocity decreased during flowing of the membrane module. Also, the velocity increased as it was near from the reactor wall to the central axis. The calculated shear stress on the membrane surface showed the highest value at the center part of the module bottom side and increased as aeration rate increased. Especially, the wall shear stress increased dramatically as the aeration rate increased from 0.15 to 0.25 L/min.

Development of Anti-disaster System for Natural Gas Governor Station Using Wire and/or Wireless Communication ($\cdot$무선 데이터 통신을 이용한 천연가스 정압소의 안전방재 시스템 개발)

  • Yoo Hui Ryong;Park Dae Jin;Koo Sung Ja;Park Seoung Soo;Rho Yong Woo
    • Journal of the Korean Institute of Gas
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    • v.3 no.2 s.7
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    • pp.17-23
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    • 1999
  • The wire and/or wireless data communication system for anti-disaster system of natural gas governor station was developed. In oder to prevent accidents of governor station, the operator was replaced by RTU(Remote Terminal Unit) which gather and transmit safety situation of governor station. The database and MMI(Man Machine Interface) were also developed to analyze the situation of governor station. The data communication between server and RTU was designed to switch automatically from wire to wireless communication and vice versa when one of them failed communication. We also have developed the patrol car management system which was applied GPS(Global Position System)/GIS(Geometric Information System), and the earthquake detection/transmission system which was adopted three dimension acceleration sensor. When a earthquake may occur, the earthquake detection/transmission system monitors data such as PGA(Peak Ground Acceleration), Sl(Spectrum Intensity) and orders the emergency shutoff valve close immediately.

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Implementation on SVM based Step Detection Analyzer (SVM 기반의 걸음 검출 분석기의 구현)

  • An, Kyung Ho;Kim, En Tae;Ryu, Uk Jae;Chang, Yun Seok
    • Journal of Korea Multimedia Society
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    • v.16 no.10
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    • pp.1147-1155
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    • 2013
  • In this study, we designed and implemented a step detection analyzer that can compare and analyze the step detection rates and results among the step detection algorithms. The step detection analyzer converts 3-axes accelerometer data into continuous energy stream through SVM operation, shows the horizontal comparison among the step detection results for each step detection algorithms, and can make elemental detection analyses. For these processes, the step detection analyzer presents the continuous energy stream as energy waveform, checks the peak values and time location of the detected steps with step detection algorithms, and gives visual interface to get some possible causes in cases of step detection miss. It can also give the threshold graph for each algorithm to check the threshold value on missed cases directly and can help to get more appropriate threshold values or other adjustable parameters in step detection algorithm. This step detection analyzer can be applied efficiently on performance enhancement of step detection algorithm, on deciding an appropriate algorithm for a specific step counter system in the various step counter filed operations.

Development of Gait Analysis Algorithm for Hemiplegic Patients based on Accelerometry (가속도계를 이용한 편마비 환자의 보행 분석 알고리즘 개발)

  • 이재영;이경중;김영호;이성호;박시운
    • Journal of the Institute of Electronics Engineers of Korea SC
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    • v.41 no.4
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    • pp.55-62
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    • 2004
  • In this paper, we have developed a portable acceleration measurement system to measure acceleration signals during walking and a gait analysis algorithm which can evaluate gait regularity and symmetry and estimate gait parameters automatically. Portable acceleration measurement system consists of a biaxial accelerometer, amplifiers, lowpass filter with cut-off frequency of 16Hz, one-chip microcontroller, EEPROM and RF(TX/RX) module. The algerian includes FFT analysis, filter processing and detection of main peaks. In order to develop the algorithm, eight hemiplegic patients for training set and the other eight hemiplegic patients for test set are participated in the experiment. Acceleration signals during 10m walking were measured at 60 samples/sec from a biaxial accelerometer mounted between L3 and L4 intervertebral area. The algorithm, detected foot contacts and classified right/left steps, and then calculated gait parameters based on these informations. Compared with video data and analysis by manual, algorithm showed good performance in detection of foot contacts and classification of right/left steps in test set perfectly. In the future, with improving the reliability and ability of the algerian so that calculate more gait Parameters accurately, this system and algerian could be used to evaluate improvement of walking ability in hemiplegic patients in clinical practice.

Design of a Compact GPS/MEMS IMU Integrated Navigation Receiver Module for High Dynamic Environment (고기동 환경에 적용 가능한 소형 GPS/MEMS IMU 통합항법 수신모듈 설계)

  • Jeong, Koo-yong;Park, Dae-young;Kim, Seong-min;Lee, Jong-hyuk
    • Journal of Advanced Navigation Technology
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    • v.25 no.1
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    • pp.68-77
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    • 2021
  • In this paper, a GPS/MEMS IMU integrated navigation receiver module capable of operating in a high dynamic environment is designed and fabricated, and the results is confirmed. The designed module is composed of RF receiver unit, inertial measurement unit, signal processing unit, correlator, and navigation S/W. The RF receiver performs the functions of low noise amplification, frequency conversion, filtering, and automatic gain control. The inertial measurement unit collects measurement data from a MEMS class IMU applied with a 3-axis gyroscope, accelerometer, and geomagnetic sensor. In addition, it provides an interface to transmit to the navigation S/W. The signal processing unit and the correlator is implemented with FPGA logic to perform filtering and corrrelation value calculation. Navigation S/W is implemented using the internal CPU of the FPGA. The size of the manufactured module is 95.0×85.0×.12.5mm, the weight is 110g, and the navigation accuracy performance within the specification is confirmed in an environment of 1200m/s and acceleration of 10g.

Development of an Intelligent Legged Walking Rehabilitation Robot (지능적 족형 보행 재활 보조 로봇의 개발)

  • Kim, Hyun;Kim, Jung-Yup
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.41 no.9
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    • pp.825-837
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    • 2017
  • This paper describes a novel type of a walking rehabilitation robot that applies robot technologies to crutches used by patients with walking difficulties in the lower body. The primary features of the developed robot are divided into three parts. First, the developed robot is worn on the patient's chest, as opposed to the conventional elbow crutch that is attached to the forearm; hence, it can effectively disperse the patient's weight throughout the width of the chest, and eliminate the concentrated load at the elbow. Furthermore, it allows free arm motion during walking. Second, the developed robot can recognize the walking intention of the patient from the magnitude and direction of the ground reactive forces. This is done using three-axis force sensors attached to the feet of the robot. Third, the robot can perform a stair walking function, which can change vertical movement trajectories in order to step up and down a single stair according to the floor height. Consequently, we experimentally showed that the developed robot can effectively perform walking rehabilitation assistance by perceiving the walking intention of the patient. Moreover we quantitatively verified muscle power assistance by measuring the electromyography (EMG) signals of the muscles of the lower limb.