This study was undertaken to investigate the effects of added phytate and pH on the solubility and in vitro digestibility of low-phytate rapeseed protein isolate. Phytate content of low-phytate rapeseed protein isolate was 1.5%, as a result of 66% removal from defatted rapeseed flour and the protein: phytate ratio was 58:1. Solubility of rapeseed protein isolate at pH 2.0 and pH 11.5 was much higher than near the isoelectric point, pH 5.0. It's solubility was lowered by adding an increased amount of phytate especially at pH 2.0. The inhibitory effect of phytate toward pepsin digestibility of rapeseed protein isolate decreased by the increasing amount of phytate added. It is suggested that the production of low-phytate rapeseed protein isolate is necessary to improve the functionality and nutritional value in order to utilize it as food material.
To investigate the comparison of dietary status and health behaviors according to obesity, 239 male workers were selected and classified as normal (18.5-22.9 27.2%), overweight (23-24.9, 37.7%), and obese (25-29.9, 35.2%) by body mass index ($kg/m^2$). The SAS (ver. 9.2) program was used and verified by the chi-square and f-value methods. Drinking frequency(2-3 times a week) was higher in normal males(45.3%), but not as high as in obese males (48.1%) (p<0.001). Smoking frequency and amount were the highest in overweight males, but not-quit-smoking was high in obese males(51.9%) (p<0.001). Exercise time was longer in normal males(108 minutes) than other groups(69 overweight males, and 82 obese males (p<0.01). Obese groups(73.8%) slept well (p<0.001), but overweight males(44.4%) showed less than 6 hours of sleep (p<0.01). Meal frequency differed by group(two meals a day 67.7% in normal males (p<0.001), no-snack 65.5% in obese males(p<0.001). The frequency of eating-out was once a day in normal males (38.5%), differed in the eating-out time (lunch(45.8%) in normal males, dinner in overweight males(52.1%) and obese males(59.5%) (p<0.01). Korean food (49.3%) was selected, but noodle differed by group(10.2% normal 21.5% obese (p<0.01). Self-perception of body differed from the body's actual condition(p<0.001). For weight control, exercise(56.4%) was practiced more than diet(18.6%). Nutrition knowledge was poor (correct answer rate was 36.7% in normal males, 41.7% in overweight males, and 46.7% in obese males). For eating attitudes, obese males answered more in "flexible to change eating habits", "supplemented when poor eating"(p<0.001), normal responded in "impact on nutrition to health", "try new food for health"(p<0.01). From these results, it is evident that male workers, especially overweight ones, must work to learn more about health and nutrition so as to combat chronic diseases.
Park, Chae-Hong;Park, Myung-Hwan;Choi, Dong-Ho;Choi, Hyung-Joo;Lee, Joon-Heon;Lee, Myung-Hoon;Hwang, Soon-Jin
Korean Journal of Ecology and Environment
/
v.46
no.1
/
pp.116-127
/
2013
At weekly intervals, we monitored continuous changes in water quality by constructed floating wetland equipped with the four different filter media (sponge, volcanic stone, activated carbon and magnesium hydroxide) in a eutrophic lake from March 2011 to May 2012. We also investigated phyto- and zooplankton communities both in the influent and the effluent water through the floating wetland. Over a 10-month time period, average turbidity (66%), suspended solids (79%) and chlorophyll-a (80%) concentrations were remarkably reduced in the effluent water compared to the influent (P<0.001). The average removal rates of $NO_2-N$ and $NH_3-N$ were 24% and 20%, respectively (P<0.05). The average removal rates of $NO_3-N$ and TN were less than 10% (P>0.05). On the other hand, the average removal rates of $PO_4-P$ and TP were more than 65% (P<0.01). Interestingly, the abundance of phytoplankton in the effluent was decreased about 2.6 times compared to that of the influent, whereas the abundance of zooplankton in the effluent was increased about 3.5 times compared to that of the influent. Overall, particulate matters (SS, Chl-a and TP) and dissolved nutrients ($NO_2-N$, $NH_3-N$ and $PO_4-P$) were particularly reduced at high rates. Therefore, application of our constructed floating wetland in a eutrophic lake improved the water quality and demonstrated a potential for algal bloom mitigation.
An, Hi-Soo;Kim, Ki-Hyoung;Park, Seung-Soo;Park, Kwang-Kyu;Kim, Young-Ho
Journal of Korean Society for Atmospheric Environment
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v.24
no.4
/
pp.415-422
/
2008
This study was carried out to investigate the effect of operating and design conditions of gas dispersion type of absorber on $SO_2$ removal efficiency. pH difference between upper and lower part of gas dispersing plate of absorber was 0.2, which was relatively low. This was supposed that recirculation capacity of absorbing liquid between froth zone and reaction zone of absorber be increased by oxidation air injection through liquid riser which acted as liquid pump. Test results showed that $SO_2$ removal efficiency was more sensitive than absorber ${\Delta}P$. High $SO_2$ removal even at lower pH resulted from very low concentration of $HSO_3^-$ ion in absorbing liquid because of direct supply of dissolved oxygen into froth zone. 96% of $SO_2$ removal efficiency was obtained under the condition of absorber pH 5.2, flue gas flow rate of $1,530\;Nm^3/hr$, inlet $SO_2$ concentration of 800 ppm, absorber ${\Delta}P$ of 250mmAq. The following equation by a multiple linear regression was obtained to describe the relationship between $SO_2$ removal and operating variables. $$f=1-{\exp}(-1.3939+1.060pH+0.0139{\Delta}P-0.00267G-0.000064SO_2Conc.),\;R^2=0.9719$$
Journal of The Korean Society of Grassland and Forage Science
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v.32
no.1
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pp.59-74
/
2012
Buffer solubility and protein fractionation were evaluated from the hays (timothy, alfalfa and klein) and straws (tall fescue and rice), and $In$$vitro$ trial was conducted to examine the effect of buffer extraction on fermentation characteristics, degradability and gas ($CO_2$ and $CH_4$) production. Buffer soluble protein (SP) content and A fraction in total protein were highest in alfalfa hay as 61% and 41.77%, respectively while lowest in rice straw (42.8% and 19.78%, respectively). No difference was observed in B1 fraction among forages but B2 fraction was slightly increased in klein hay (12.34%) and tall fescue straw (10.05%) compared with other forages (6.34~8.85%). B3 fraction of tall fescue was highest as 38.49% without difference among other forages while C fraction was highest in rice straw. pH in incubation solution was higher in all forages after extraction than before extraction at 3h (P<0.01) and 6h (P<0.05), and pH from hays of timothy and alfalfa was higher than the other forages at 6h (P<0.05) and 12h (P<0.001). Regardless of extraction, ammonia-N concentration from alfalfa hay was increased at all incubation times and extraction effect was appeared only at 3h incubation time (P<0.01). Total VFA concentration from alfalfa hay was highest up to 24h incubation while those from tall fescue straw and rice straw were lowest. Buffer extraction decreased (P<0.01~P<0.001) the total VFA concentration. Acetic acid proportion was increased (P<0.001) before extraction of forages but no difference was found between forages. Propionic acid($C_3$) proportion was also increased(P<0.001) before extraction in all forages than in straws at 3h, 24h and 48h incubations, and $C_3$ from hays were mostly higher (P<0.05) than from straws. Butyric acid proportion, however, was not affected by extraction at most incubation times. Parameter 'a' regarding to the dry matter (DM) degradation was increase (P<0.001) in all forages before extraction, and was decreased (P<0.05) in tall fescue straw and rice straw compared with hays. Parameter 'b' was also increased (P<0.001) before extraction but no difference was found between forages. Effective degradability of DM (EDDM) was higher (P<0.001) before extraction in most forages except for rice straw. Buffer extraction decreased (P<0.05) all parameters (a, b, and c) regrading to the crude protein (CP) degradation but no difference was found between forages. Effective degradation of CP (EDCP) was lower (P<0.05) in straws than in hays. Parameters 'a' and 'b' regarding to the NDF degradation (P<0.01) and effective degradability of NDF (EDNDF, P<0.001) were also higher in forages before extraction than after extraction but no difference was found between forages. Buffer extraction reduced (P<0.05~P<0.001) $CO_2$ production from all the forages uo to 24h incubation and its production was greater (P<0.05~P<0.01) from hays than straws. Methane ($CH_4$) production was also greater (P<0.01~P<0.001) in all forages at all incubation times, and its production was greater (P<0.05) from hays than from straws at most incubation times. Based on the results of the current study, it can be concluded that buffer solubility and CP fractionation might be closely related with $In$$vitro$ VFA concentration, degradability and gas ($CO_2$ and $CH_4$) production. Thus, measurement of buffer solubility and protein fractionation of forages might be useful to improve TMR availability in the ruminants.
Journal of the Korea Academia-Industrial cooperation Society
/
v.17
no.2
/
pp.179-187
/
2016
This study was conducted to identify the factors that affect the nursing performance. These factors are general characteristics, mentoring function and organizational citizenship behavior. A survey was conducted on 163 nurses working for general hospitals in South Korea from December 2 - 30, 2015. The data was analyzed using the SPSS WIN 21.0 program to analyze the descriptive statistics, independent T-test, ANOVA, Scheffe test, Pearson's correlation, and multiple regression. The factors affecting the subject's nursing performance were altruism (${\beta}=.46$, p<.001), courtesy (${\beta}=.12$, p=.047), belonging to the sub areas of the organizational citizenship behaviors, role modeling function (${\beta}=.31$, p=.001), career develop functions (${\beta}=-.22$, p=.018), belonging to the sub areas of the mentoring function, and position (${\beta}=.24$, p<.001). The results of this study are expected to provide basic data to improve the nursing performance.
The objective of the current study was to determine the effect of pre-slaughter stress, season and breed on the activity of plasma creatine kinase (CK) and the quality of mutton. One hundred and seventy-three (173) castrated sheep from Dormer (DM), South African Mutton Merino (SAMM), Dorper (DP) and Blackhead Persian (BP) sheep breeds were used in the study. The animals were grouped according to age-groups as follows: Group 1 (6 to 8 months), Group 2 (9 to 12 months) and Group 3 (13 to 16 months). Blood samples were collected during exsanguinations using disposable vacutainer tubes for CK analysis. Representative samples of the Muscularis longissimuss thoracis et. lumborum (LTL) were collected from 84 castrated sheep, of different breeds (28 per breed) 24 h after slaughter. The following physico-chemical characteristics of mutton were determined; meat pH ($pH_{24}$), color ($L^*$, $a^*$ and $b^*$), thawing and cooking losses and Warner Braztler Shear Force (WBSF). The activity of plasma CK was significantly higher (p<0.001) in summer ($1,026.3{\pm}105.06$) and lower in winter ($723.3{\pm}77.75$). There were higher values for $L^*$ ($33.7{\pm}0.94$), $b^*$ ($11.5{\pm}0.48$) and WBSF ($29.5{\pm}1.46$) in summer season than in winter season; $L^*$ ($29.4{\pm}0.64$), $b^*$ ($10.2{\pm}0.33$) and WBSF ($21.2{\pm}0.99$). The activity of plasma CK was influenced by the type of breed with Dormer having the highest (p>0.001) levels ($1,358.6{\pm}191.08$) of CK. South African Mutton Merino had higher values for $pH_{24}$ ($5.9{\pm}0.06$), $L^*$ ($34.2{\pm}0.97$), $b^*$ ($12.2{\pm}0.50$) and WBSF ($26.8{\pm}1.51$) and Blackhead Persian had higher values ($35.5{\pm}2.17$) for cooking loss (CL%) than the other breeds. Computed Principal Component Analyses (PCA) on the activity of plasma CK and physico-chemical characteristics of mutton revealed no correlations between these variables. However, positive correlations were observed between $pH_{24}$, $L^*$, $a^*$, $b^*$, CL% and WBSF. Relationships between pre-slaughter stress, CK activity and physico-chemical characteristics of mutton were also observed. It was therefore concluded that although mutton quality and creatine kinase were not related, pre-slaughter stress, season and breed affected the activity of creatine kinase and mutton quality.
Lee, Seok Hee;Ha, Se Eun;Lee, Jun Kyoung;Park, Jong Kun
Journal of Life Science
/
v.24
no.2
/
pp.112-117
/
2014
We investigated the effects of extracts from Rubus coreanus (RC) and Artemisia princeps var. orientalis (AP) on DNA damage response in ultraviolet B (UVB)-exposed HaCaT cells. Cell activity upon treatment for 24 h with RC or AP alone was similar to or greater than that of the nontreated control. When UVB-exposed cells were postincubated for 24 h in medium containing RC or AP, cell activity increased in a concentration-dependent manner. Nuclear fragmentation analysis showed that postincubation with RC or AP decreased UVB-induced apoptosis by about 20% and 15%, respectively, of that in cells postincubated with growth medium. When UVB-exposed cells were postincubated for 24 h in medium containing RC or AP, the level of cyclobutane pyrimidine dimer decreased in a concentration- dependent manner. Western blot analysis showed that treatment of cells not exposed to UVB with RC or AP alone did not significantly change the levels of phospho-p53 and GADD45 protein. Interestingly, when UVB-exposed cells were postincubated for 24 h in medium containing RC or AP, phospho-p53 and GADD45 levels decreased in a concentration dependent manner. Our results suggest that RC and AP extract assist the survival of UVB-exposed cells in parallel with a decrease in levels of UVB-induced DNA damage and damage-response proteins, such as p53 and GADD45.
Kim, Sung-Il;Chun, Suk-Keun;Park, Du-Goan;Park, Keun-Ho;Park, So-Jin;Kim, Chul-Ung
Journal of the Korean Applied Science and Technology
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v.24
no.2
/
pp.140-148
/
2007
As an additional high purification method of p-dioxanone monomer for a high molecular weight polymer, the sweating operation of crystalline layer obtained by layered melt crystallization from p-dioxanone-diethylene glycol system was studied. Purity and yield of p-dioxanone crystal depended mainly on the sweating temperature and sweating time. Increasing sweating time and sweating temperature, the purity of p-dioxanone crystal increase, whereas the yield of that decrease, respectively. Through the optimization of sweating operation, p-dioxanone crystal can be upgraded to very high purity over 99.9 % suited to monomer for polymerization.
Hwang, Jung Hye;An, Sang Mi;Park, Da Hye;Kang, Deok Gyeong;Kim, Tae Wan;Park, Hwa Chun;Ha, Jeongim;Kim, Chul Wook
Journal of the Korean Society of International Agriculture
/
v.30
no.4
/
pp.277-284
/
2018
Meat quality has always been one of the most important factors that controls the choice of pork consumers and is of great interest in the pig industry. In this study, we identified a single nucleotide polymorphism (SNP) in the enoyl-CoA delta isomerase 2 (ECI2) gene in Berkshire pigs (n = 430) by analyzed the association between the SNP and meat quality traits. The non-synonymous SNP in the ECI2 gene is located at c.608 C > G and resulted in an amino acid change from threonine to serine. Significant associations between the SNP and meat quality traits, such as redness (CIE a), and the $pH_{24hr}$, were revealed in both the dominant and co-dominant models, whereas carcass weight, drip loss, and fat content and moisture content were significantly associated only with the dominant model. In barrow, the SNP was significantly associated with CIE a, drip loss, and $pH_{24hr}$, whereas in gilt, only a significant relationship with moisture content was observed. GG genotype pigs had a higher $pH_{24hr}$ and lower drip loss. Because $pH_{24hr}$ and drip loss are considered the most important meat quality traits, GG genotype pork is high-quality meat compared to that from other genotypes. In conclusion, the SNP in the ECI2 gene is significantly associated with several meat quality traits. These traits and could be as genetic markers in molecular breeding programs for improving meat quality.
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