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검색결과 431건 처리시간 0.031초

삼백초(三白草)가 복강(腹腔) 대식세포(大食細胞)로부터 Nitric Oxide(NO) 유리기전(遊離機轉)에 대한 연구(硏究) (Studies on the mechanism of Nitric oxide (NO) induction in the Peritoneal Macrophage by HERBA SAURUI (HS))

  • 전길환;신민교;송호준
    • 대한한의학회지
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    • 제19권2호
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    • pp.36-49
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    • 1998
  • HERBA SAURURI (HS) has been known to use antiinflammatory drug. To investigated the mechanism of HS-induced NO synthesis, I evaluated the ability of protein kinase C (PKC) inhibitors such as staurosporine (STSN) or polyymyxin B to block HS-induced effects. HS alone had only a small effect, whereas in combination with $rIFN-{\gamma}$, markedly increased NO synthesis in a dose dependent manner. STSN and polymyxin B decreased NO synthesis, which had been induced by $rIFN-{\gamma}$, plus HS. Furthermore, prolonged incubation of the cells with phorbol ester, which down-regulates PKC activity abolished synergistic cooperative effect of HS with $rIFN-{\gamma}$ on NO synthesis. STSN and Polymyxin B potently inhibited HS-induced $TNF-{\alpha}$ secretion by $rIFN-{\gamma}$ plus HS. However, $rIFN-{\gamma}$ plus $TNF-{\alpha}-induced$ NO synthesis was not blocked by STSN or polymyxin B. On the other hand, tyrosine kinase inhibitor, genistein, blocked the NO synthesis and $TNF-{\alpha}$ secretion by $rIFN-{\gamma}$ plus HS. In conlusion, the present results strongly suggest that the capacity of HS to increase NO synthesis from $rIFN-{\gamma}-primed$ macrophages is the result of HS-induced $TNF-{\alpha}$ secretion via the signal transduction pathway of PKC and tyrosine kinase.

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좌귀음가미방이 실험적 재생불량성빈혈에 미치는 영향 (Effects of Juakium-derivative on Experimental Aplastic Anemia)

  • 박신명;김영철;이장훈;우흥정
    • 대한한의학회지
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    • 제23권1호
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    • pp.133-144
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    • 2002
  • Objectives : Korean traditional medicinal herbs have been used to improve the function of the hematopoietic system. The purpose of this study is to examine the hematopoietic effects of Juakium-derivative on aplastic anemia. Methods : In vitro, after bone marrow cells of mice with aplastic anemia induced by benzene were cultured with Juakium-derivative, we measured the gene expressions of hematopoietic cytokines, the intracellular TPO and SCF expression and the colony number of CFU-GEMM and BFU-E. Results : The results are summarized as follows : 1. The gene expressions of hematopoietic cytokines (TPO, c-mpl, SCF, c-kit, IL-3, EPO, EpoR, GM-CSF) and the TPO and SCF expression of the group treated with Juakium-derivative increased significantly more than those of the control group. 2. The colony number of CFU-GEMM and BFU-E in the rEPO plus rIL-3 plus Juakium-derivative group increased significantly compared with the only rEPO plus rIL-3 group. Conclusion : It was acknowledged that Juakium-derivative has hemato-potentiating effects on aplastic anemia induced by benzene and it is expected that Juakium-derivative can be used clinically for the patient with hematopoietic system disorder. This study could also be used further as important research data for Korean oriental medicine about hemato-potentiating. The exact mechanism explaining our study would need to be studied further.

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Comparison of Ramosetron Plus Dexamethasone with Ramosetron Alone on Postoperative Nausea, Vomiting, Shivering and Pain after Thyroid Surgery

  • Lee, Myeong Jong;Lee, Kyu Chang;Kim, Hye Young;Lee, Won Sang;Seo, Won Jun;Lee, Cheol
    • The Korean Journal of Pain
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    • 제28권1호
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    • pp.39-44
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    • 2015
  • Background: Postoperative nausea and vomiting (PONV), postanesthetic shivering and pain are common postoperative patient complaints that can result in adverse physical and psychological outcomes. Some antiemetics are reported to be effective in the management of postoperative pain and shivering, as well as PONV. We evaluated the efficacy of dexamethasone added to ramosetron on PONV, shivering and pain after thyroid surgery. Methods: One hundred and eight patients scheduled for thyroid surgery were randomly allocated to three different groups: the control group (group C, n = 36), the ramosetron group (group R, n = 36), or the ramosetron plus dexamethasone group (group RD, n = 36). The patients were treated intravenously with 1 and 2 ml of 0.9% NaCl (group C); or 2 ml of 0.15 mg/ml ramosetron plus 1 ml of 0.9% NaCl (group R); or 2 ml of 0.15 mg/ml ramosetron plus 1 ml of 5 mg/ml dexamethasone (group RD) immediately after anesthesia. Results: Incidence of nausea and the need for rescue antiemetics, verbal rating scale (VRS) 1 hour pain value, ketorolac consumption, and incidence of shivering were significantly lower in group R and group RD, than in group C (P < 0.05). Moreover, these parameters were significantly lower in group RD than in group R (P < 0.05). Conclusions: Combination of ramosetron and dexamethasone significantly reduced not only the incidence of nausea and need for rescue antiemetics, but also the VRS 1 hour pain value, ketorolac consumption, and the incidence of shivering compared to ramosetron alone in patients undergoing thyroid surgery.

분자선 에피성장법으로 성장된 ZnSe/GaAs의 특성 (Property of molecular beam epitaxy-grown ZnSe/GaAs)

  • 김은도;손영호;조성진;황도원
    • 한국결정성장학회지
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    • 제17권2호
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    • pp.52-56
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    • 2007
  • 본 연구에서는 초고진공(UHV, ultra high vacuum) 분자선 에피성장(MBE, molecular beam epitaxy) 시스템을 제작하여, ZnSe/GaAs[001]을 증착하였고, 증착된 박막의 특성을 SEM(scanning electron microscopy), AFM(atomic force microscopy)으로 조사하여, 분자층 단위의 조밀하고 균일한 표면특성을 보이고 있음을 확인할 수 있었다 XRD(x-ray diffractometer)를 이용하여, GaAs[001]기판의 XRD peak 위치와 ZnSe 박막의 XRD peak 위치가 각자 일치함을 확인할 수 있었다. PL(photoluminescence)로는 대략 437nm에서 발광하는 것이 관측되었으며, 2인치 ZnSe 박막의 PL mapping을 측정하였다.

백굴채가 대식세포의 NO 및 $TNF-{\alpha}$ 생성에 미치는 영향 (The Effects of Chelidonium majus on NO and $TNF-{\alpha}$ Production in Macrophages)

  • 김홍준;문석재;김동웅;문구;원경숙;윤준철;김유경;원진희
    • 대한한의학회지
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    • 제24권2호
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    • pp.138-147
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    • 2003
  • Objectives : In this study, we investigated the mechanism by which Chelidonium majus (CM) regulates nitric oxide (NO) production. Methods : Using mouse peritoneal macrophages, the mechanism by which CM regulates NO or tumor necrosis $factor-{\alpha}(TNF-{\alpha})$ production was examined. NO release was measured by the Griess method. $TNF-{\alpha}$ production was measured by the ELISA method. The protein extracts were prepared and samples were analyzed for the inducible NOS(iNOS) expression and nuclear factor kappa $B(NF-{\kappa}B)$ activation by Western blotting. Results : When CM was used in combination with recombinant $interferon-{\gamma}{\;}(rIFN-{\gamma})$, there was a marked cooperative induction of NO production. CM had an effect on NO production by itself. The expression of the iNOS gene was increased in $rIFN-{\gamma}$ plus CM-stimulated peritoneal macrophages and almost completely inhibited by pre-treatment with pyrrolidine dithiocarbamate (PDTC), an inhibitor of $NF-{\kappa}B$. The $NF-{\kappa}B$ activation was increased in rIFN-{\gamma} plus CM-induced peritoneal macrophages. The increased production of NO from $rIFN-{\gamma}$ plus CM-stimulated peritoneal rnacrophages was decreased by the treatment with $N^{G}-monomethyl-{_L}-arginine{\;}(N^{G}MMA){\;}N^{\alpha}-Tosyl-Phe$ chloromethyl ketone (TPCK) , and was almost completely inhibited by pre-treatment with PDTC. Furthermore, treatment with CM alone or rIFN-{\gamma} plus CM in peritoneal macrophages caused a significant increase in $TNF-{\alpha}$ production. PDTC decreased CM-induced $TNF-{\alpha}$ production significantly. After CM treatment in HT-29 or AGS cells, cell viability decreased. Conclusions : These findings demonstrate that CM increases the production of NO and $TNF-{\alpha}{\;}by{\;}rIFN-{\gamma}-primed$ macrophages and suggest that NF-B plays a critical role in mediating these effects of CM.

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Glucagon-like peptide-1 and glucagon-like peptide-1 receptor agonists in the treatment of type 2 diabetes

  • Lee, Seungah;Lee, Dong Yun
    • Annals of Pediatric Endocrinology and Metabolism
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    • 제22권1호
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    • pp.15-26
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    • 2017
  • The prevalence of type 2 diabetes (T2D) is increasing worldwide. Patients with T2D suffer from various diabetes-related complications. Since there are many patients with T2D that cannot be controlled by previously developed drugs, it has been necessary to develop new drugs, one of which is a glucagon-like peptide-1 (GLP-1) based therapy. GLP-1 has been shown to ameliorate diabetes-related conditions by augmenting pancreatic ${\beta}-cell$ insulin secretion and having the low risk of causing hypoglycemia. Because of a very short half-life of GLP-1, many researches have been focused on the development of GLP-1 receptor (GLP-1R) agonists with long half-lives such as exenatide and dulaglutide. Now GLP-1R agonists have a variety of dosing-cycle forms to meet the needs of various patients. In this article, we review the physiological features of GLP-1, the effects of GLP-1 on T2D, the features of several GLP-1R agonists, and the therapeutic effect on T2D.

Selenium 과 Vitamin E 투여가 한우 종모우의 정액성상, 혈액성분 및 호르몬 변화에 미치는 효과 II. Selenium, Vitamin E 및 rBST 투여가 한우 종모우의 혈중내 Selenium 과 Vitamin E 의 농도 변화에 미치는 효과 (Effects of Selenium and Vitamin E Administration on the Semen Characteristics, Blood Chemical Values and Hormone in Hanwoo Sires II. Changes of Selenium and Vitamin E Concentrations of Blood in Hanwoo Sires after Selenium, Vitamin E and rBST Administration)

  • 박동헌;양부근;김종복;전기준;정희태;박춘근;이성수;박노형;원유석
    • 한국가축번식학회지
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    • 제23권3호
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    • pp.205-212
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    • 1999
  • 본 연구는 rBST, Vit. E 및 Se의 투여가 한우 종모우의 혈청내 Se과 vitamin E의 농도 변화에 미치는 영양을 검토하였다. 1. rBST, Vit. E 및 Se 투여가 한우 종모우의 혈청 내 Se농도 변화에 미치는 영향을 조사한 결과, 혈청내 Se 농도는 Vit. E와 Se 혼합투여구가74.09ppb/ml로서 대조구(64.55ppb/ml)와 rBST투여구(65.50ppb/ml) 및 vit. E 투여구(68.15ppb/m1)보다 높은 농도를 나타냈으나(P<0.05), selenium 투여구(73.11ppb/ml)와는 통계적 유의차가 없었다(P>0.05). 2. rBST, Vit. E 및 Se 투여가 한우 종모우의 혈청 내 Vit. E 농도 변화에 미치는 영향을 조사한 결과, 혈청내 Vit. E농도는 Vit. E투여구와 혼합투여구가 각각 2.80 및 2.75ppm/ml로서 여타구(대조구, 2.27 ; rBST투여구, 2.32 및 Se투여구, 2.58ppm/rnl)보다 다소 높게 나타났지만 통계적 유의차는 인정되지 않았다(P>0.05). 3. Vt. E 투여구, Se 투여구 및 혼합투여구에서 투여 횟수에 따른 혈청내 Se과 Vit. E의 농도 변화는 모든 투여구에서 투여 횟수가 증가함에 따라 혈청내 농도가 증가하였다.

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플러스 사이즈 여성용 신발 라스트 개발을 위한 발 특성 연구 (A Study on the Foot Characteristics for the Development of the Last Footwear for Plus Size Women)

  • 김남순;도월희
    • 한국의류산업학회지
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    • 제22권3호
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    • pp.378-385
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    • 2020
  • The purpose of this study was to present basic data for development of shoe last suitable for women of plus size (BMI 25 kg/㎡ or higher) by type and to analyze the characteristics of each type of foot type. The results of the study are as follows. A comparison of the feet of a plus-size woman and a normal-weight woman showed that the feet of a plussize woman were thicker and wider in the toes, cheeks and feet than those of a normal-weight woman, while the ankle area was thicker. As a result of the cluster analysis by type of foot type of plus-size woman, type 1 was classified into four types, type 1 was 49 (19.5%), type 2 was 43 (17.1%), type 3 was 53 (21.1%), and type 4 was distributed among 106 (42.2%). Type 1 appeared to be a long, low heel, thick foot and wide H-type, and Type 2 appeared to be a D-type with a low heel and a thinner and narrow foot compared to other types, but with wide outer width of the foot. Type 3 showed short feet, low heels, narrow feet, but slanted sides, and Type 4 showed type A with long feet, high heels, thick and wide feet or a combination of toes.

Effects of Selected Inoculants on Chemical Compositions and Fermentation Indices of Rye Silage Harvested at Dough Stage

  • Lee, Seong Shin;Paradhipta, Dimas H.V.;Joo, Young Ho;Lee, Hyuk Jun;Kwak, Youn Sig;Han, Ouk Kyu;Kim, Sam Churl
    • 한국초지조사료학회지
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    • 제38권2호
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    • pp.99-105
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    • 2018
  • This study was carried out to estimate the effect of selected inoculants on chemical compositions and fermentation characteristics of rye silage. Rye was harvested at dough stage and divided into 5 treatments, following: No additives (CON); L. plantarum R48-27 (LP27); L. buchneri R4-26 (LB26); Mixture of LP27 and LB26 at 1:1 ratio (MIX); and L. buchneri (LB). The rye forage was ensiled into 10 L bucket silo for 100 days. The contents of NDF and ADF were lowest (P<0.05) in LB26. The pH in LB26, MIX, and LB were lower (P<0.05) than CON and LP27. Lactate content in LB was higher (P<0.05) than the others, while acetate content in LB26 and LB were higher (P<0.05) than that in CON and LP27. Lactate to acetate ratio was highest (P<0.05) in LB, but lowest in LB26. Lactic acid bacteria (LAB) count in LB was higher (P<0.05) than that in CON, while yeast count in CON was lower than in all silages applied inoculants. In conclusion, silages inoculated with LB26 could improve potentially the aerobic stability caused by increases of acetate and propionate concentrations.