DOI QR코드

DOI QR Code

Effects of Selected Inoculants on Chemical Compositions and Fermentation Indices of Rye Silage Harvested at Dough Stage

  • Lee, Seong Shin (Division of Applied Life Science (BK21Plus, Insti. of Agri. & Life Sci.), Gyeongsang National University) ;
  • Paradhipta, Dimas H.V. (Division of Applied Life Science (BK21Plus, Insti. of Agri. & Life Sci.), Gyeongsang National University) ;
  • Joo, Young Ho (Division of Applied Life Science (BK21Plus, Insti. of Agri. & Life Sci.), Gyeongsang National University) ;
  • Lee, Hyuk Jun (Division of Applied Life Science (BK21Plus, Insti. of Agri. & Life Sci.), Gyeongsang National University) ;
  • Kwak, Youn Sig (Department of Plant Medicine (Insti. of Agri. & Life Sci.), Gyeongsang National University) ;
  • Han, Ouk Kyu (Central Area Crop Breeding Division, National Institute of Crop Science, RDA) ;
  • Kim, Sam Churl (Division of Applied Life Science (BK21Plus, Insti. of Agri. & Life Sci.), Gyeongsang National University)
  • Received : 2018.02.13
  • Accepted : 2018.06.11
  • Published : 2018.06.30

Abstract

This study was carried out to estimate the effect of selected inoculants on chemical compositions and fermentation characteristics of rye silage. Rye was harvested at dough stage and divided into 5 treatments, following: No additives (CON); L. plantarum R48-27 (LP27); L. buchneri R4-26 (LB26); Mixture of LP27 and LB26 at 1:1 ratio (MIX); and L. buchneri (LB). The rye forage was ensiled into 10 L bucket silo for 100 days. The contents of NDF and ADF were lowest (P<0.05) in LB26. The pH in LB26, MIX, and LB were lower (P<0.05) than CON and LP27. Lactate content in LB was higher (P<0.05) than the others, while acetate content in LB26 and LB were higher (P<0.05) than that in CON and LP27. Lactate to acetate ratio was highest (P<0.05) in LB, but lowest in LB26. Lactic acid bacteria (LAB) count in LB was higher (P<0.05) than that in CON, while yeast count in CON was lower than in all silages applied inoculants. In conclusion, silages inoculated with LB26 could improve potentially the aerobic stability caused by increases of acetate and propionate concentrations.

Keywords

References

  1. Adesogan, A.T., Krueger, N., Salawu, M.B., Dean, D.B. and Staples, C.R. 2004. The influence of treatment with dual-purpose bacterial inoculants or soluble carbohydrates on the fermentation and aerobic stability of bermudagrass. Journal of Dairy Science. 87:3407-3416. https://doi.org/10.3168/jds.S0022-0302(04)73476-1
  2. AOAC. 1990. Official method of analysis, 15th ed. Association of Official Analytical Chemists, Arlington, Virginia, USA.
  3. Arasu, M.V., Ilavenil, S., Jane, M., Kim, D.H., Lee, K.D., Park, H.S. and Choi, K.C. 2014. Effect of addition of chlorella with Lactobacillus plantarum on quality, microbial contents and fermentation metabolites of barley and pea silages. Journal of Pure Applied Microbiology. 8:4017-4023.
  4. Baah, J., Addah, W., Okine, E.K. and McAllister, T.A. 2011. Effects of homolactic bacterial inoculant alone or combined with an anionic surfactant on fermentation, aerobic stability and in situ ruminal degradability of barley silage. Asian-Australasian Journal of Animal Sciences. 24:369-378. https://doi.org/10.5713/ajas.2011.10320
  5. Casler, M.D. and Vogel, K.P. 1999. Accomplishments and impact from breeding for increased forage nutritional value. Crop Science. 39:12-20. https://doi.org/10.2135/cropsci1999.0011183X003900010003x
  6. Chaney, A.L. and Marbach, E.P. 1962. Modified reagents for determination of urea and ammonia. Clinical Chemistry. 8:130-132.
  7. Cho, S.B., Kang, J.S., Cho, K.J., Lee, K.H., Kwon, C.H., Song, J.Y., Lee, K.H., Kim, S.Y. and Kim, E.J. 2014. Effect of homofermentative and heterofermentative lactic acid bacteria on the quality and aerobic stability of silage: Meta-Analysis. Journal of the Korean Society of Grassland and Forage Science. 34:247-253. https://doi.org/10.5333/KGFS.2014.34.4.247
  8. Choi, K.C., Ilavenil, S., Arasu, M.V., Park, H.S. and Kim, W.H. 2015. Effect of addition of lactic acid bacteria on fermentation quality of rye silage. Journal of the Korean Society of Grassland and Forage Science. 35:277-282. https://doi.org/10.5333/KGFS.2015.35.4.277
  9. Choi, K.C., Soundarranjan, I., Srisesharam, S., Park, H.S., Kim, J.H., Jung, J.S. and Kim, H.S. 2016. Potential effects of novel lactic acid bacteria on fermentation quality of rye haylage. Journal of the Korean Society of Grassland and Forage Science. 36:23-28. https://doi.org/10.5333/KGFS.2016.36.1.23
  10. Cleale, R.M.I.V. and Bull, L.S. 1986. Effect of forage maturity on ration digestibility and production by dairy cows. Journal of Dairy Science. 69:1587-1594. https://doi.org/10.3168/jds.S0022-0302(86)80575-6
  11. Courtin, M.G. and Spoelstra, S.F. 1990. A simulation model of the microbiological and chemical changes accompanying the initial stage of aerobic deterioration of silage. Grass and Forage Science. 45:153-165. https://doi.org/10.1111/j.1365-2494.1990.tb02196.x
  12. Demirci, U., Gulsen, N. and Keles, G. 2011. Effects of bacterial inoculants on fermentation and aerobic stability of baled triticale hungarian vetch silage and lamb performance. Kafkas Universitesi Veteriner Fakultesi Dergisi. 17:297-302.
  13. Elferink, S.J.O., Krooneman, J., Gottschal, J.C., Spoelstra, S.F., Faber, F. and Driehuis, F. 2001. Anaerobic conversion of lactic acid to acetic acid and 1, 2-propanediol by Lactobacillus buchneri. Applied and Environmental Microbiology. 67:125-132. https://doi.org/10.1128/AEM.67.1.125-132.2001
  14. Filya, I., Ashbell, G., Hen, Y. and Weinberg, Z.G. 2000 The effect of bacterial inoculants on the fermentation and aerobic stability of whole crop wheat silage. Animal Feed Science and Technology. 88:39-46. https://doi.org/10.1016/S0377-8401(00)00214-5
  15. Han, O.K., Hwang, J.J., Park, H.H., Kim, D.W., Oh, Y.J., Park, T.I., Ku, J.H., Kwon, Y.U., Kweon, S.J. and Park, K.G. 2015. A new high grain yielding forage rye cultivar, "Seedgreen". Journal of the Korean Society of Grassland and Forage Science. 35:105-111. https://doi.org/10.5333/KGFS.2015.35.2.105
  16. Hu, W., Schmidt, R.J., McDonell, E.E., Klingerman, C.M. and Kung, Jr.L. 2009. The effect of Lactobacillus buchneri 40788 or Lactobacillus plantarum MTD-1 on the fermentation and aerobic stability of corn silages ensiled at two dry matter contents. Journal of Dairy Science. 92:3907-3914. https://doi.org/10.3168/jds.2008-1788
  17. Ilavenil, S., Arasu, M. V., Vijayakumar, M., Jung, M.W., Park, H.S., Lim, Y.C. and Choi, K.C. 2014. Lactobacillus plantarum improves the nutritional quality of Italian Ryegrass with alfalfa mediated silage. Journal of the Korean Society of Grassland and Forage Science. 34:174-178. https://doi.org/10.5333/KGFS.2014.34.3.174
  18. Ju, J.I., Choi, H.G., Kang, Y.S., Lee, J.J., Park, K.H. and Lee, H.B. 2009. Changes of growth and forage yield at different cutting dates among five winter cereals for whole crop silage in middle region. Journal of the Korean Society of Grassland and Forage Science. 29:111-120. https://doi.org/10.5333/KGFS.2009.29.2.111
  19. Kaiser, A.G., Piltz, J.W., Burns, H.M. and Griffiths, N.W. 2004. Successful silage (2nd Ed). Dairy Australia and New South Wales Department of Primary Industries. Australia. pp. 27.
  20. Kang, T.W., Adesogan, A.T., Kim, S.C. and Lee, S.S. 2009. Effects of an esterase-producing inoculant on fermentation, aerobic stability, and neutral detergent fiber digestibility of corn silage. Journal of Dairy Science. 92:732-738. https://doi.org/10.3168/jds.2007-0780
  21. Keady, T.W.J., Steen, R.W.J., Kilpatrick, D.J. and Mayne, C.S. 1994. Effects of inoculant treatment on silage fermentation, digestibility and intake by growing cattle. Grass and Forage Science. 49:284-294. https://doi.org/10.1111/j.1365-2494.1994.tb02003.x
  22. Kim, D.A., Sung, K.I. and Kwon, C.H. 1986. Effects of sowing time and seeding rate on growth characteristics, winter survival and dry matter yield of forage rye. Journal of the Korean Society of Grassland and Forage Science. 6:164-168.
  23. Kim, H.S., Han, O.K., Kim, S.C., Kim, M.J., and Kwak, Y.S. 2017. Screening and investigation Lactobacillius spp. to improve Secale cereale silage quality. Animal Science Journal. 88:1538-1546. https://doi.org/10.1111/asj.12781
  24. Kim, J.G., Chung, E.S., Seo, S., Ham, J.S., Kang, W.S. and Kim, D.A. 2001. Effects of maturity at harvest and wilting days on quality of round baled rye silage. Asian-Australasian Journal of Animal Sciences. 14:1233-1237. https://doi.org/10.5713/ajas.2001.1233
  25. Kim, K.M., Lee, B.J. and Cho, Y.S. 2012. Differences of soil carbon by green manure crops in rotated cropping system. Korean Journal of Soil Science and Fertilizer. 45:1027-1031. https://doi.org/10.7745/KJSSF.2012.45.6.1027
  26. Ko, Y.D., Kang, W.S., Kang, H.S., Kim, D.J., Kim, D.H., Kim, J.H., Nam, G.H., Ryu, Y.M., Shin, J.N. and Yoo, S.O. 1999. Manufacture strategy of silage for ruminants. Sunjin Printing. Seoul. pp. 5-41.
  27. Lee, H.I., Choi, Y.J., Mamuad, L., Kim, E.J., Oh, Y.K., Park, K.K. and Lee, S.S. 2014. Effect of heterofermentative lactic acid bacteria on the quality of Italian ryegrass and whole-crop barley silage. Journal of the Korean Society of Grassland and Forage Science. 34:269-276. https://doi.org/10.5333/KGFS.2014.34.4.269
  28. McDonald, P., Henderson, A.R. and Heron, S.J.E. 1991. The biochemistry of silage, 2nd Ed. Chalcombe Publications, Bucks, UK.
  29. Morris, H.D. and Gardner, F.P. 1985. The effect of nitrogen fertilization and duration of clipping period on forage and grain yield of oats, wheat, and rye. Agronomy Journal. 50:454-457.
  30. Nsereko, V.L., Smiley, B.K., Rutherford, W.M., Spielbauer, A., Forrester, K.J., Hettinger, G.H., Harman, E.K. and Harman, B.R. 2008. Influence of inoculating forage with lactic acid bacterial strains that produce ferulate esterase on ensilage and ruminal degradation of fiber. Animal Feed Science and Technology. 145:122-135. https://doi.org/10.1016/j.anifeedsci.2007.06.039
  31. Oba, M. and Allen, M.S. 1999. Evaluation of the importance of the digestibility of neutral detergent fiber from forage: Effects on dry matter intake and milk yield of dairy cows. Journal of Dairy Science. 82:589-596. https://doi.org/10.3168/jds.S0022-0302(99)75271-9
  32. Oh, M.G., Jo, I.H. and Hwangbo, S. 2014. Effect of mixed-sowing of legume and applying of cattle manure on the productivity, feed values and organic Hanwoo carrying capacity of rye (Secale cereale L.). Korean Journal of Organic Agriculture. 22:457-468. https://doi.org/10.11625/KJOA.2014.22.3.457
  33. Park, C.M., Chun, W.B. and Myung, G.H. 1979. Studies on the utilization of some soiling grasses. Journal of Animal Science and Technology. 21:289-298.
  34. SAS User's Guide, Version 8 Edition. 2002. SAS Inst., Inc., Cary, NC. USA.
  35. Seale, D.R. 1986. Bacterial inoculants as silage additives. Journal of Applied Bacteriology. 61(Suppl.):9S-26S.
  36. Song, B.H., Lee, K.A., Jeon, W.T., Kim, M.T., Cho, H.S., Oh, I.S., Kim, C.G. and Kang, U.G. 2010. Effects of green manure crops of legume and gramineae on growth responses and yields in rice cultivation with respect to environment friendly agriculture. Korean Journal of Crop Science. 55:144-150.
  37. Song, T.H., Han, O.K., Yun, S.K., Park, T.I., Seo, J.H., Kim, K.H. and Park, K.H. 2009. Changes in quantity and quality of winter cereal crops for forage at different growing stages. Journal of the Korean Society of Grassland and Forage Science. 29:129-136. https://doi.org/10.5333/KGFS.2009.29.2.129
  38. Tilley, J.M.A. and Terry, R.A. 1963. A two-stage technique for the in vitro digestion of forage crops. Grass and Forage Science. 18:104-111. https://doi.org/10.1111/j.1365-2494.1963.tb00335.x
  39. Van Soest P.J., Robertson, J.B. and Lewis, B.A. 1991. Methods for dietary fiber, neutral detergent fiber and non-starch poly-saccharides in relation to animal nutrition. Journal of Dairy Science. 74:3568-3597.
  40. Vetter, R.L. and Von Glan, K.N. 1978. Abnomal silages and silage related disease problems. National Feed Ingredients Association. Des Mlines. IA. pp. 291-293.
  41. Zahiroddini, H., Baah, J., Absalom, W. and McAllister, T.A. 2004. Effect of an inoculant and hydrolytic enzymes on fermentation and nutritive value of whole crop barley silage. Animal Feed Science and Technology. 117:317-330. https://doi.org/10.1016/j.anifeedsci.2004.08.013