The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
권호 표지

Studies on the mechanism of Nitric oxide (NO) induction in the Peritoneal Macrophage by HERBA SAURUI (HS)

삼백초(三白草)가 복강(腹腔) 대식세포(大食細胞)로부터 Nitric Oxide(NO) 유리기전(遊離機轉)에 대한 연구(硏究)

  • Published : 1998.10.30
Download PDF
⟨ Previous Next ⟩

Abstract

HERBA SAURURI (HS) has been known to use antiinflammatory drug. To investigated the mechanism of HS-induced NO synthesis, I evaluated the ability of protein kinase C (PKC) inhibitors such as staurosporine (STSN) or polyymyxin B to block HS-induced effects. HS alone had only a small effect, whereas in combination with $rIFN-{\gamma}$, markedly increased NO synthesis in a dose dependent manner. STSN and polymyxin B decreased NO synthesis, which had been induced by $rIFN-{\gamma}$, plus HS. Furthermore, prolonged incubation of the cells with phorbol ester, which down-regulates PKC activity abolished synergistic cooperative effect of HS with $rIFN-{\gamma}$ on NO synthesis. STSN and Polymyxin B potently inhibited HS-induced $TNF-{\alpha}$ secretion by $rIFN-{\gamma}$ plus HS. However, $rIFN-{\gamma}$ plus $TNF-{\alpha}-induced$ NO synthesis was not blocked by STSN or polymyxin B. On the other hand, tyrosine kinase inhibitor, genistein, blocked the NO synthesis and $TNF-{\alpha}$ secretion by $rIFN-{\gamma}$ plus HS. In conlusion, the present results strongly suggest that the capacity of HS to increase NO synthesis from $rIFN-{\gamma}-primed$ macrophages is the result of HS-induced $TNF-{\alpha}$ secretion via the signal transduction pathway of PKC and tyrosine kinase.

Keywords

References

  1. 大韓植物圖鑑 李昌福
  2. 貴州中藥名錄刪 貴州省中醫硏究所
  3. 中草藥彩色圖譜與驗方 舒普榮
  4. 七00－治癌中藥及其處方 葉銘洪
  5. 中國常用中草藥 沈保安
  6. 福建省中藥炮制規範 王岳寶(外 4名)
  7. 抗癌中藥方選 王冰
  8. 本草綱目 李時珍
  9. 本草藥材圖鑑
  10. 中國藥物大全（中藥卷） 中國藥物大全編委會
  11. 中藥志（第四冊） 中國醫學科學院藥物硏究所
  12. 中華人民共和國藥典 中華人民共和國衛生部藥典委員會
  13. 中華人民共和國藥典 中華人民共和國衛生部藥典委員會
  14. 河南省中藥材炮制規範 河南省衛生廳
  15. 本草學 全國韓醫科大學 本草學 敎授(共編)：
  16. 中藥大辭典 江蘇新醫學院
  17. 原色天然藥物大辭典 (下卷） 金在吉
  18. 雲南中藥志 (第一卷） 黎光南
  19. 中藥大辭典 第二卷 上海科學技術出版社
  20. 中草學 成都中醫學院
  21. 原色實用臺灣靑草藥 吳盛義
  22. 圖解鄕藥（生藥）大辭典(植物篇） 辛民敎;鄭普燮
  23. 中國本草圖錄 卷四 中國本草圖錄 編寫委員會
  24. 中國方藥學 戴新民
  25. 治癌中草藥 林秀英
  26. 中國草藥手冊 中國生草藥硏究發展中心
  27. 藥用植物鍾子手冊 孫昌高
  28. 藥用植物學 丁景和
  29. 草木便方 劉善述
  30. 植物名實圖攷長編(校印) 吳其濬
  31. 唐 · 新修本草(輯復本） 蘇敬;李勣
  32. 臨床本草學（改訂增補版） 辛民敎
  33. 新編中醫學槪要 廣東中醫學院
  34. 福建藥物志 福建省醫學硏究所
  35. 臺灣藥用植物誌（第一卷） 甘偉松
  36. 新 藥品植物學 藥品植物學硏究會
  37. 本草綱目通釋 陳貴廷
  38. 中華藥海 冉善德
  39. 常用中藥成分與藥理手冊 黃泰康
  40. 現代中藥藥理學 王本祥
  41. 抗癌中草藥大辭典 劉春安;彭明
  42. Pharmacol. Rev. v.43 Nitric oxide: physiology, pathophysiology, and Pharmacology. Moncada, S.;R. M. J. Palmer;E. A. Higgs.
  43. Cell v.70 Nitric oxide, a novel biological messenger. Lowenstein, C. J.;S. H. Snyder.
  44. J. Biol. Chem. v.266 Purification of nitric oxide synthase from rat macrophages. Yui,.Y.;R. Hattori, ;K. Kosuga;H. Eizawa;K. Hiki;C. Kawai
  45. Anal. Biochem. v.126 Analysis of nitrite, nitrate, and [$^{23}$N] nitrate in biological fluids. Green, L. C.;D. A. Wanger;J. Glogowski;P. L. Skipper;J. S. Wishnok;S. R. Tannenbaum.
  46. J. Biol. Chem. v.261 Down regulation of phorbol diester: receptors by proteolytic degration of protein kinase C in cultured cell line of fetal rat skin keratinocytes. Chida, K.;N. Kato;T. Kuroli.
  47. Lancet v.2 Rasied serum levels of tumor necrosis factor in Parasitic infections. Scuderi, P.;R. E. Sterling;K. S. Lam;P. R. Finley;K J. Ryan;C. G. Ray;E, Petersen; D. J. Slymen;S. E. Salmon.
  48. J. Biol. Chem. v.266 Membrane interactions of amphilicpolypeptides mastoparan, melittin, polymyxin B, and cardiotoxin: differential inhibition of Protein kinase C, Ca$^{2+}/calmodulin-dependent protein kinase Ⅱ and synaptosomal membrane Na. K-ATPase, and Na$^{+} pump and differentiation of HL60 cells. Raynor, R. L,;B. Zheng;J. F. Kuo.
  49. J. Biol. Chem. v.261 Down regulation of phorbol diester receptors by proteolytic degration of protein kinase C in cultured cell line of fetal rat skin keratinocytes. Chida, K.;N. Kato;T. Kuroki.
  50. Annu. Rev. Immunol. v.7 The biology of cachectin/TNF-${\alpha}$ primary mediator of the host response. Beutler B;Cerami A.
  51. Nature v.326 Tumor necrosis factor : polypeptide mediator network. Old, L. J
  52. J. Clin. Invest. v.79 Secretory products of macrophages. Nathan, C. F.
  53. J. Immunol. v.144 Tumor necrosis factor signal transduction: cell type-specific activation and translocation of protein kinase C. Schutze, S.;S. Nottrott;K Pfizenmaier;M. Kronke.
  54. J. Immunol. v.153 Synergistic cooperation between phorbol ester and interferon- ${\gamma}$ for induction of nitric oxide synthesis in murine peritoneal macrophages. Jun, C. D.;B. M. Choi;H. Ryu;J. Y. Um;H. J. Kwak;B. S. Lee;S. G. Paik;H. M.
  55. J. Exp. MEd. v.177 Lipopolysaccharideinduced selective pr-iming effect on tumor necrosis factor-${\alpha}$ and nitric oxide production in mouse peritoneal macrophages. Zhang X.;Morrison D. C.
  56. J. Exp. Med. v.177 Lipopolysaccharide-induced selective priming effects on tumor necrosis factor-${\alpha}$ and nitric oxide production in mouse peritoneal macrophages. Zhang X.;Morrison D. C.
  57. Trends Biochem. Sci. v.19 Signaling by the cytokine receptor superfamily: JAKs and STATs. Ihle J. N.;Witthuhn B. A.;Quelle F. W.(et. at.)