• BMB Reports
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• v.43 no.5
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• pp.344-348
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• 2010

Messenger ribonucleoprotein particles (mRNPs) are used to transport mRNAs along neuronal dendrites to their site of translation. Staufen2 is an mRNA-binding protein expressed in the cell bodies and cellular processes of different brain cells. It is notably involved in the transport of dendritic mRNAs along microtubules. Its knockdown expression was shown to change spine morphology and impair synaptic functions. However, the identity of Staufen2-bound mRNAs in brain cells is still completely unknown. As a mean to identify these mRNAs, we immunoprecipitated Staufen2-containing mRNPs from embryonic rat brains and used a genome wide approach to identify Staufen2-associated mRNAs. The genome wide approach identified 1780 mRNAs in Staufen2-containing mRNPs that code for proteins involved in cellular processes such as post-translational protein modifications, RNA metabolism, intracellular transport and translation. These results represent an additional and important step in the characterization of Staufen2- mediated neuronal functions in rat brains.

• Journal of the Korean Applied Science and Technology
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• v.28 no.4
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• pp.379-385
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• 2011

One of main catalysts for De-NOx in SCR is a $V_2O_5/TiO_2$, and this work formulated powdery catalysts focusing ultimately on corrugate catalytic support. The prepared catalyst consisted of anatase $TiO_2$. Amount of the added vanadium oxide determined the viscosity of catalyst slurry, which is important for washcoat for a final corrugate type catalytic reactor. The test showed a proportional relation between adsorption amount of ammonia and specific surface area. De-NOx efficiency could be obtained up to 96.3 % at $400^{\circ}C$ with a spacial velocity of $4,000hr^{-1}$.

• Journal of Power System Engineering
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• v.20 no.4
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• pp.69-74
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• 2016

Recently, in order to meet the stricter emission regulations, the proportion of after-treatments for vehicle and vessel is increasing gradually. The purpose of this study is to investigate the de-$CH_4$ characteristics of NGOC in front of proposed combined system according to additive catalyst and support ratio. In the case of Pd addition, the de-$CH_4$ performance of 2Pt-2Pd-3MgO/$Al_2O_3$ NGOC was improved by approximately 10 to 20% for the HC components. The de-$CH_4$ performance of 2Pt-2Pd-3Cr-3MgO/$Al_2O_3$ NGOC was higher compared to five kinds of NGOC catalysts, because Cr particle was smaller and dispersion of Pd was increased. The NGOC(Zeolite:$Al_2O_3$(80%:20%)｝catalyst according to support ratio, was improved performance at low temperature region on CO and NO conversion rate.

• Journal of the Chungcheong Mathematical Society
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• v.23 no.4
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• pp.679-689
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• 2010

By developing and using certain operators like those initiated by Burchnall-Chaundy, the authors aim at investigating several decomposition formulas associated with the $Kamp{\acute{e}}$ de $F{\acute{e}}riet$ function $F_{2:0;0}^{0:3;3}$ [x, y]. For this purpose, many operator identities involving inverse pairs of symbolic operators are constructed. By employing their decomposition formulas, they also present a new group of integral representations of Eulerian type for the $Kamp{\acute{e}}$ de $F{\acute{e}}riet$ function $F_{2:0;0}^{0:3;3}$ [x, y], some of which include several hypergeometric functions such as $_2F_1$, $_3F_2$, an Appell function $F_3$, and the $Kamp{\acute{e}}$ de $F{\acute{e}}riet$ functions $F_{2:0;0}^{0:3;3}$ and $F_{1:0;1}^{0:2;3}$.

• Polymer(Korea)
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• v.32 no.5
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• pp.446-457
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• 2008

Three kinds of hydroxypropyl cellulose (HPC) derivatives: 6- (cholesteryloxycarbonyl) pentoxypropyl celluloses(CHPCs) with degree of esterification(DE) ranging from 0.6 to 3, 6-[4-{4'-(nitrophenylazo)phenoxycarbonyl}] pentoxypropyl celluloses (NHPCs) with DE ranging from 0.4 to 3, and fully 6-(cholesteryloxycarbonyl) pentanoated NHPCs (CNHPCs) were synthesized, and their thermotropic liquid crystalline properties were investigated. All the CHPCs and NHPCs with $DE{\leq}1.7$ formed enantiotropic cholesteric phases, whereas CNHPCs with 6-(cholesteryloxycarbonyl) pentanoyl DE(DEC) more than 1.6 exhibited monotropic cholesteric phases. On the other hand, NHPCs with $DE{\geq}2.4$ and CNHPCs with $DEC{\leq}1.3$ showed monotropic nematic phases. NHPCs with $DE{\leq}l$, as well as HPC, formed right-handed helices whose optical pitches (${{\lambda}_m}'s$) increase with temperature, while all the CHPCs formed left-handed helices whose ${{\lambda}_m}'s$ decrease with temperature. In contrast with these derivatives, NHPCs with $1.4{\leq}DE{\leq}1.7$ and CNHPCs with $DEC{\geq}1.6$ did not display reflection colors over the full cholesteric range, suggesting that the helical twisting power of the cellulose chain and the cholesteryl group highly depends on the chemical structure and DE of mesogenic group.

• Horticulture, Environment, and Biotechnology : HEB
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• v.59 no.6
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• pp.815-826
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• 2018

The intense drought affecting olive production in Northern Chile underscores the need to research non-traditional irrigation strategies to obtain the best crop performance. Accordingly, this study aimed to obtain preliminary data to guide future research on this topic. Different water replenishment levels on crop evapotranspiration ($ET_c$ ; 13.5, 27.0, 40.5, and 54%) were established in a young orchard, cv. Arbequina, from the end of fruit drop (EFD) to full bloom in the next season. We evaluated the influence of plant water status (${\Psi}_{stem}$ ) and crop load, considered as function of fruit number divided by trunk cross-sectional area, on reproductive and productive variables using multiple linear regressions. Our results show that crop load and ${\Psi}_{stem}$ measured from EFD to harvest affected yield components. Nevertheless, ${\Psi}_{stem}$ had the strongest influence on fruit size, pulp development, oil accumulation, and yield. Oil content and yield were reduced by 54% and 50% for each MPa, respectively, from ${\Psi}_{stem\;EFD-H}$ -1.8 MPa, an effect that intensified as crop load increased. During the period of flower development (September-November), the number of flowers per inflorescence and percentage of perfect flowers were reduced when ${\Psi}_{stem}$ was less than -2.0 MPa. These preliminary results showed that bud differentiation, inflorescence and flower formation are highly sensitive to water deficit.

• Journal of Veterinary Science
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• v.24 no.5
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• pp.54.1-54.13
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• 2023

Background: Porcine reproductive and respiratory syndrome virus (PRRSV) vaccines do not provide full cross-protection, mainly due to the virus genetic variability. Despite this, vaccines based on modified-live PRRSV (PRRSV-MLV) reduce the disease impact. Objectives: To assess the efficacy of two commercial vaccines-one based on PRRSV1 (PRRSV1-MLV) and another on PRRSV2 (PRRSV2-MLV)-against a Japanese PRRSV2 field strain. Methods: Two groups of three-week-old piglets were vaccinated (G1: PRRSV1-MLV; G2: PRRSV2-MLV) and two were kept as non-vaccinated (INF and CTRL). One month later, G1, G2, and INF were challenged with a PRRSV2 field strain. Results: After the challenge, clinical signs were only observed in INF. Moreover, the highest rectal temperatures and values for the area under the curve (AUC) were observed in INF. Regarding viral detection, both AUC and the proportion of positive samples in blood were higher in INF. In G1, viremic animals never reached 100%. At necropsy (21 d after the challenge), differences for titers among groups were only found in tonsils (G1 < G2 and INF). One animal (belonging to G1) was negative in all tissues. Regarding humoral responses, G1 and G2 seroconverted after vaccination, as detected in the corresponding enzyme-linked immunosorbent assay. Specific neutralizing antibodies (NA) against PRRSV1-MLV were already detected at 14 d after vaccination in G1, showing a significant booster after the challenge, while PRRSV2-MLV NA were detected in G2 at the end of the experiment. Conclusions: Despite genetic differences, PRRSV1-MLV has been demonstrated to confer partial protection against a Japanese PRRSV2 strain, at least as good as PRRSV2-MLV.

• Fisheries and Aquatic Sciences
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• v.1 no.2
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• pp.255-259
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• 1998

This study was designed to evaluate the economic analysis of channel catfish production in 1998 based on fish value and total feed cost. Catfish received higher protein feeds with lesser amount based on the dietary protein levels, but received the constant total protein input for all treatments. Weight gain per pond for treatment 1 $(28\%\;protein,\;100\%\;of\;satiation)$ was higher (P<0.10) than for treatment 3 $(36\%\;protein,\;77.8\%\;of\;satiation)$, but not significantly higher than for treatment 2 $(32\%\;protein,\;87.5\%\;of\;satiation)$ at constant DE. At constant DE/P (treatments 4, 2 and 5), weight gain per pond for treatment 5 $(36\%\;protein,\;77.8\%\;of\;satiation)$ was lower (P<0.10) than for treatment 2, but not significantly lower than for treatment 4 $(28\%\;protein,\;100\%\;of\;satiation)$. At constant DE, feed conversion slightly improved as dietary protein level increased from $28\%\;to\;32\%$ and feed allowance decreased by $12.5\%$, but did not improve further as dietary protein level increased from $28\%\;to\;36\%$ and feed allowance decreased by $22.2\%$. At constant DE/P, feed conversion improved as dietary protein level increased from $28\%\;to\;32\%$ increased and feed allowance decreased by $12.5\%$, but did not improve as dietary protein level increased from $28\%\;to\;36\%$ and feed allowance decreased by $22.2\%$ Total feed cost for treatment 1 was slightly, but not significantly higher than for treatments 2 and 3 at constant DE. At constant DE/P, total feed cost for treatment 5 was higher (P<0.05) than for treatment 2, but not significantly higher than for treatment 4. Total value of fish ($ /ha) produced for treatment 1 was highest and lowest was for treatment 5. Return above feed cost was highest for treatment 1 and nearly the same as treatment 2. Return over feed cost for treatments 3 and 4 were slightly lower than for treatments 1 and 2. Economic analysis showed that feeding fish the diet containing $28\%$ protein and 3.08 kcal/g DE to satiation and the diet containing $32\%$ protein and 3.08 kcal/g DE to $87.5\%$ of satiation produced the highest profit to farmer.

• Plant Biotechnology Reports
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• v.5 no.2
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• pp.177-186
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• 2011

This study describes for the first time in Pinus genus a plant regeneration system via a combined pathway of somatic embryogenesis and organogenesis from immature seeds of radiata pine. Somatic embryos were obtained from embryogenic line 2162 of Pinus radiata D. Don on EDM basal medium containing $60{\mu}M$ ABA and 6% sucrose. The explants used for organogenesis experiments were either freshly collected somatic embryos or somatic embryos germinated for 1 week. Germination medium was half-strength LP medium, supplemented with 0.2% activated charcoal. Different induction periods and BA concentrations were assayed for shoot induction. After induction treatments, explants were elongated on the same medium used for germination stage. Rooting medium was quarter-strength LP medium supplemented with three different auxin treatments: $1.5mg\;L^{-1}$ 1-naphthalene acetic acid (NAA), $1.5mg\;L^{-1}$ indole-3-butyric acid (IBA) and $1mg\;L^{-1}$ IBA with $0.5mg\;L^{-1}$ NAA (MIX). The effect of the photon flux ($120mmol\;m^{-2}\;s^{-1}$ and darkness) in the first week of the explants in the rooting media was also tested. This methodology could offer an alternative to overcome some problems associated with somatic embryogenesis such as the seasonality of embryogenic tissue (ET) initiation or a low embryo production from the ET, a particularly important issue in the case of genetically transformed ETs.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.1
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• pp.72-81
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• 2009

Eight Black Belly rams (45.2 kg) fitted with permanent ruminal cannulae were used in a 2${\times}$2 factorial design to determine the effects of feeding frequency and regrowth age on intake and digestion. Rams were fed with 21- or 35-day old fresh pangola grass offered ad libitum two or four times a day. Irrespective of the regrowth age, there was a tendency for intake to be positively correlated with increase in meal frequency. Differences were not significant (p>0.25). Significant effects of meal frequency were observed in NDF and ADF total tract digestibility of the 35-day grass which decreased as the number of meals increased. Meal frequency had no visible effect on feeding behaviour. Total rumen content increased when animals were fed twice a day as opposed to four times a day. Similarly, an accumulation of small and very small particles was observed in the rumen of rams fed twice a day in comparison with those fed four times a day. These results suggest that studies of digestive dynamics performed at a steady state are not representative of the rumen loading observed in farm rams which have two important peaks of meal.