• 한국지역사회생활과학회지
• /
• 제21권1호
• /
• pp.13-18
• /
• 2010

This study was performed to investigate the antimicrobial effects of Euphorbia humifusa ethanol-extract against food-borne pathogens. The growth inhibitory effects of the extract at a concentration of 250, 500, 1,000 or 2,000 mg/L on food poisoning microorganism were determined against Salmonella typhimurium, Listeria monocytogenes, Yersinia enterocolitica, Escherichia coli O157:H7 and Staphylococcus aureus. The microorganisms growth was not affected by the extract at the concentration up to 250 mg/L, but was significantly (p<0.05) inhibited by the extract at a concentration higher than 1,000 mg/L. The extract of Euphorbia humifusa had strong antimicrobial activity against all test strains at a concentration of 2,000 mg/L. The results in the present study demonstrate antimicrobial effects of Euphorbia humifusa ethanol-extract against food-borne pathogens, suggesting that Euphorbia humifusa could be an effective natural antibacterial agent in food.

• Korean Journal of Acupuncture
• /
• 제19권1호
• /
• pp.67-75
• /
• 2002

목적 : 자성(雌性) 흰쥐에서 phenylhydrazine으로 유발된 용혈성 빈혈에 오미자 약침의 항빈혈효과를 알아보기 위해서 적혈구수, hematocrit ratio, hemoglobin, 혈청 철함유량 및 serum total iron binding capacity (TIBC)를 관찰하였다. 방법 : 실험동물은 정상군, 대조군, 250 mg/kg 오미자 투여군 및 500 mg/kg 오미자 투여군으로 총 4개의 군으로 분류하였다. 정상군의 흰쥐는 아무런 처치도 하지 않았고, 대조군은 phenylhydrazine으로 용혈성 빈혈을 유발하였다. 오미자 투여군은 용혈성 빈혈을 유발한 후 7일간 매일 250 mg/kg 및 500 mg/kg의 용량으로 중완혈에 자침하였다. 결과 : 적혈구수, hematocrit ratio 및 hemoglobin은 대조군에서 정상군에 비해 감소한 반면 오미자 투여군은 증가하였다. 혈청 철함유량과 TIBC는 대조군에서 증가하였으나, 오미자 투여군에서는 감소하였다. 이러한 결과로 중완혈에 오미자 자침은 phenylhydrazine으로 유발된 용혈성 빈혈에서 항빈혈효과가 있는 것으로 사려된다.

• 약학회지
• /
• 제46권6호
• /
• pp.411-415
• /
• 2002

We compared the hypoglycemic effects of Ginseng Radix Alba (GRA) and Mori Folium (MF) in multiple low dose (MLD) streptozotocin(STZ)-induced diabetic rats. In order to induce hyperglycemic state 25 mg/kg of STZ was injected intraperitoneally for 5 consecutive days. SD rats were randomly divided into diabetic control and treatment groups. Treatment groups were administered with either 500 mg/kg of GRA (G500), 500 mg/kg of MF (M500), or 250 mg/kg of GRA mixed with same dose of MF (GM250) for 3 weeks. Blood glucose level and body weight were measured every 5th day. G500 and M500 both significantly reduced blood glucose levels as compared to the diabetic control group (diabetic control, 458.3$\pm$25.4 mg/dl; G500, 275.0$\pm$12.0; M500, 278.0$\pm$15.4; GM250, 324.0$\pm$18.4). While body weight in diabetic control group was decreased slightly after 3 weeks, treatment groups showed gradual increases of body weight during 3 week-period. Plasma insulin level was increased by treatment with GRA, but those levels in M500 and GM250 groups were similar to the diabetic control (normal control, 32.0$\pm$13.9 $\mu$IU/mι; diabetic control, 12.4$\pm$1.9; G500, 17.5$\pm$3.4; M500, 11.1$\pm$3.2; GM250, 10.5$\pm$t3.7). Urine glucose levels were also remarkably reduced in all treatment groups (normal control, 0.0$\pm$0.0 g/day; diabetic control, 11.4$\pm$2.5; G500, 4.9$\pm$0.2; M500, 5.7$\pm$1.6 ; GM250, 8.2$\pm$0.2). At the second and third week of the treatment, food and water intakes were determined. At the third week, food and water intakes were significantly decreased in all treatment groups. Taken together, we may conclude that GRA and MF alone may prevent or delay the development of hyperglycemia, however, synergistic hypoglycemic activity was not be seen in group treated with mixed formula of GRA and MF when compared to GRA or MF alone.

• 약학회지
• /
• 제50권5호
• /
• pp.313-321
• /
• 2006

There are three kinds of liquid petroleum marker which is extracted by the basic or acidic, and both developer. Korean marker, Unimark 1494 DB (marker) have been spectrophotometrically analysed by the determination of absorbance at 582 nm after base extraction by Unimark 1494 DB Developer C-5 (developer). Some blue dyes which have same reactive radical of marker and can be changed deep blue color in base developer extraction (BDE), may be increased absorbance at 582 nm, but dyes or markers which can be increased the absorbance, were not unclear. In this experiment, effects of three dyes or marker such as Orimax Green 151 (the mixture of CI Solvent Yellow 16 and CI Solvent Blue 70), quinizarin and Orimax Blue 2N (CI Solvent Blue 35), and two solvent such as topasol (P-250) and lubricant (P-8) on the absorbance were studied by HITACHI Recording Spectrophotometer U-3300. It shows that all of them increased absorbance at 582 nm after BDE. Absorbance at 582 nm can be showed 0.0544 by Orimax Green 151 at the concentration of 3.96 mg/l, quinizarin at the concentration of 1.38 mg/l, and Orimax Blue 2N at the concentration of 2.73 mg/l in the artificial petroleum (normal diesel oil: topasol: lubricant=2 : 4: 4), respectively. Absorbance, 0.0544 indicates that concentration of marker is 1.64 mg/l in the reference curves, respectively. And also these results can be showed that the artificial petroleum have about 10% cheep fuel such as kerosene which have marker (16.0 mg/l). Absorbance of P-250 was 0.01361-0.22842 depending upon the purchasing date, and that of P-8 was 0.05644. pH of developer was 14.83, and so this result indicates that Unimark 1494 DB is a base extractable petroleum marker, phenylazophenol (US Patent No. 5,252,106). In the BDE, the slight color of Orimax Blue 2N, Orimax Green 151 and quinizarin in artificial petroleum changed to deep bright blue color, respectively. These result indicate that the absorbance at 582 nm by BDE may be increased not only by azo, diazo, amine and ketone (anthraquinone, coumarin) dyes or markers, but also the contaminants of P-250 and P-8 which have same as reactive radical of dyes or markers.

• 동의생리병리학회지
• /
• 제26권1호
• /
• pp.67-73
• /
• 2012

The exposure of gastric mucosa to ethanol produces acute ulcers mediated by inflammatory processes, hemorrhagic erosions and increase of reactive oxygen species. The purpose of this study was to assess the effects of Coptidis Rhizoma(CR) aqueous extracts on hydrochloride (HCl)/ethanol induced gastric ulcer in mice as compared with rebamipide (30 mg/kg) and ranitidine (100 mg/kg). Stomach ulcers were induced by oral ingestion of HCl/ethanol. CR extracts (125, 250 and 500 mg/kg) were orally administered, once a day for 7 continuous days, and 1 hr after last 7th treatment of CR extracts stomach ulcers were induced. Effects of CR extracts on HCl/ethanol-induced gastric ulcer were evaluated based on gross and microscopic observations with anti-oxidant activities. All three different dosages of CR extract significantly decreased HCl/ethanol-induced gastric ulcer compared with the HCl/ethanol control mice. CR extracts also strengthened the antioxidative defense systems - decreased the level of lipid peroxidation but increased the level of catalase, superoxide dismutase and nitrate/nitrite compared with the HCl/ethanol control. The effects of CR extract 500 mg/kg were similar to that of 30 mg/kg rebamipide, and CR extract 250 mg/kg showed similar anti-ulcer effects as compared with ranitidine 100 mg/kg. These results suggest that the gastroprotective effects of CR extracts on mice ulcer models can be attributed to its ameliorating effect on oxidative damages.

• 대한수의학회지
• /
• 제32권2호
• /
• pp.251-258
• /
• 1992

Stable plurilamellar vesicle(SPLV) 제작법을 이용한 liposomal cephalexin으로 젖소의 Staphylococcus aureus성 유방염에 대해 건유기 치료를 시도하였다. SPLV의 모양과 크기를 transmission electron microscopy로 관찰하였으며 SPLV의 포획율 및 안정성은 high performance liquid chromatography 및 liquid scintillation counting으로 조사한 바 SPLV의 크기는 대개 직경 $0.1{\sim}4.0{\mu}m$였고, cephalexin 포획율은 $25.8{\pm}3.1$ $(mean{\pm}SE)$였으며 $4^{\circ}C$ 보관시 0, 24, 48, 72 및 96 시간후의 유리농도백분율은 각각 1.1, 3.9, 6.5, 7.5 및 8.4%였다. S aureus에 감염된 45분방에 대하여 15분방씩 3군으로 나누어 각 분방당 250mg의 liposomal cephalexin(LC군), 250mg free cephalexin(FC)군 및 blank liposome을 첨가한 250mg cephalexin(BLC)군을 건유시 분방내 주입한 후 분만당일에 균분리 및 동정을 실시한 바 LC군, FC군 및 BLC군에서 각각 1, 8 및 7분방에서 상기와 동일한 균이 분리되어 LC군이 타군에 비해 유의한 치료효과를 나타내었다.

• 한국전자파학회논문지
• /
• 제28권4호
• /
• pp.279-285
• /
• 2017

본 연구는 인체 귓불 생체조직을 유전체로 하는 평행판 커패시터를 이용한 새로운 방식의 공진기를 제안하였으며, 비 침습 방식으로 준 마이크로파 신호를 이용하여 생체조직의 혈당변화를 관찰할 수 있는 가능성을 연구하였다. 공진기는 마이크로스트립 선로의 접지면을 활용하여 용량성 슬롯(inductive slot)과 연결된 평행판 커패시터로 구성된다. 커패시터 도체판은 인체조직을 가상한 팬텀 박스(phantom box)를 포함한다. 시뮬레이션을 토대로 제안된 공진기를 설계하여 제작하였다. 혈당 농도 수준변화를 3단계(0, 250, 500 mg/dL)에서 실험하였고, 반사계수($S_{11}$)가 측정되었다. 100 이상의 높은 Q 값을 갖는 공진기를 통해, 900 MHz 대역 부근에서 250 mg/dL의 농도 수준변화 대비 약 9 MHz의 주파수 천이가 관찰되어 혈당 센서에 적용 가능성을 입증하였다.

• 한국임상수의학회지
• /
• 제28권3호
• /
• pp.280-286
• /
• 2011

본 실험은 고지방식이로 유발된 비만마우스에서 산마늘 잎 메탄올 추출물의 항비만 효과를 알아보고자 체중변화, 사료섭취량변화, 렙틴과 아디포넥틴 수치변화, 난소주위 지방무게 변화 측정과 지방세포의 조직병리학적 검사를 실시하였으며, 메트포민을 체중당 250 mg 투여한 약물대조군과 비교평가 하였다. 비만유발을 위하여 고지방식이를 13주간 공급하였으며, 고지방식이 공급 7일째에 증류수(비만대조군), 메트포민(약물대조군), 산마늘 잎 메탄올 추출물을 투여하였다. 비만대조군은 확연한 비만이 유발되었으며, 산마늘 잎 메탄올 추출물(62.5, 125, 250 mg/kg) 투여군에서는 이러한 비만이 유의적으로 감소하였다. 결론적으로 산마늘 잎 메탄올 추출물은 비만유발 마우스에서 비만개선작용을 나타내었으며, 나아가 인체에서도 유의한 비만개선작용을 할 것으로 생각된다.

• 셀메드
• /
• 제9권1호
• /
• pp.2.1-2.6
• /
• 2019

Decoction of Cocos nucifera husk fibre is used indigenously in Nigeria for malaria treatment. Polyphenols have been identified as the phytochemicals responsible for the antimalarial activity of Cocos nucifera husk fibre, though their toxicity has not been evaluated. The polyphenols of Cocos nucifera husk fibre were therefore evaluated for their effects on selected kidney function indices in mice. Fifty mice were randomly divided into five groups (A-E) of ten mice each. Mice in group A were orally administered 5% DMSO solution while those in groups B, C, D and E were orally administered 31.25, 62.5, 125 and 250 mg/Kg body weight of the polyphenols respectively for seven days. Serum urea, creatinine and uric acid concentrations were determined. Serum levels of sodium, potassium, chloride and calcium ions and kidney alkaline phosphatase (ALP), glutamate dehydrogenase (GDH) and gamma-glutamyltransferase (GGT) activities were also determined. The results showed that the polyphenols significantly reduced (p<0.05) urea concentration at 250 mg/Kg body weight and creatinine concentration at all doses compared to controls. The polyphenols caused no significant alteration (p>0.05) in serum uric acid concentration and kidney ALP, GGT and GDH activities compared to controls. There was significant increase (p<0.05) in serum sodium ion concentration at 31.25, 125 and 250 mg/Kg body weight of polyphenols whereas significant increase (p<0.05) in serum potassium and chloride ions was observed at 62.5 and 250 mg/Kg body weight compared to controls. Thus, polyphenols of Cocos nucifera husk fibre may adversely affect some osmoregulatory functions of the kidney, especially at higher concentrations.

• 생물환경조절학회지
• /
• 제19권4호
• /
• pp.397-402
• /
• 2010

자생 산수국의 대량번식을 위하여 삽목에 적합한 생장조절물질의 종류 및 농도와 침지시간이 삽목에 미치는 영향을 조사하였다. 자생 산수국의 숙지 삽목시 생장조절물질인 IBA와 NAA를 저농도($10{\sim}250\;mg{\cdot}L^{-1}$) 및 고농도($500{\sim}3,000mg{\cdot}L^{-1}$)로 침지시간을 각각 다르게 처리하여 삽목 한 후 42일(6주)째 발근율, 발근수, 근장, 엽장, 엽폭를 조사하였다. IBA처리의 경우 발근수, 근장, 엽장, 엽폭은 저농도($50{\sim}250\;mg{\cdot}L^{-1}$)일때 우수하였다. 그 중 IBA $50mg{\cdot}L^{-1}$ 3시간 침지처리구에서 근수가 90.55개로 가장 좋았으며, IBA $10mg{\cdot}L^{-1}$일때 는 매우 저조하였으므로 산수국의 대량 번식에는 효과를 주지 못하는 것으로 판단되었다. 처리에서는 NAA $1000mg{\cdot}L^{-1}$ 5초 침지, $2000mg{\cdot}L^{-1}$ 3초 침지, $3000mg{\cdot}L^{-1}$ 10초 침지 일때 발근율 100%를 보였으나 발근율에 비해 발근수는 오히려 감소되는 것을 볼 수 있었으며, NAA는 저농도($10{\sim}250\;mg{\cdot}L^{-1}$)보다 고농도($1,000{\sim}3000mg{\cdot}L^{-1}$)일때 효과가 높았으며 고농도 중 NAA $2000mg{\cdot}L^{-1}$ 5초 침지일 때는 발근수가 105.45개로 가장 많은 발근수가 나타났다. NAA의 고농도처리에서는 농도가 높고, 순간침지는 짧을수록 효과가 가장 높은 것을 볼수 있었다. 따라서 자생 산수국의 대량번식을 위한 삽목번식에 적합한 생장조절물질은 NAA보다 IBA 저농도($50{\sim}250\;mg{\cdot}L^{-1}$)일 때 발근율 및 효과가 높았다. 이와 같이 자생 산수국의 대량번식에 적합한 생장조절 물질은 IBA가 NAA에 비해 발근율 및 발근수 등이 좋았으며, IBA $50mg{\cdot}L^{-1}$ 3시간이 삽목에 가장 효과적이었다.