한국임상수의학회지 (Journal of Veterinary Clinics)

  • 제28권3호
  • /
  • Pages.280-286
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  • 2011
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  • 1598-298X(pISSN)
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  • 2384-0749(eISSN)
한국임상수의학회 (The Korean Society of Veterinary Clinics)
권호 표지

고지방식이로 유발된 비만마우스에서 산마늘 잎추출물의 항비만효과

Allium victorialis Leaf Extract Prevents High Fat Diet Induced Obesity in Mice

  • Ku, Sae-Kwang (College of Oriental Medicine, Daegu Haany University) ;
  • Chung, In-Kwon (College of Oriental Medicine, Daegu Haany University) ;
  • Cheon, Woo-Hyun (College of Oriental Medicine, Daegu Haany University) ;
  • Kim, Joo-Wan (Glucan Corp., Research Institute, Marine Biotechnology center)
  • 심사 : 2011.06.13
  • 발행 : 2011.06.30
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초록

본 실험은 고지방식이로 유발된 비만마우스에서 산마늘 잎 메탄올 추출물의 항비만 효과를 알아보고자 체중변화, 사료섭취량변화, 렙틴과 아디포넥틴 수치변화, 난소주위 지방무게 변화 측정과 지방세포의 조직병리학적 검사를 실시하였으며, 메트포민을 체중당 250 mg 투여한 약물대조군과 비교평가 하였다. 비만유발을 위하여 고지방식이를 13주간 공급하였으며, 고지방식이 공급 7일째에 증류수(비만대조군), 메트포민(약물대조군), 산마늘 잎 메탄올 추출물을 투여하였다. 비만대조군은 확연한 비만이 유발되었으며, 산마늘 잎 메탄올 추출물(62.5, 125, 250 mg/kg) 투여군에서는 이러한 비만이 유의적으로 감소하였다. 결론적으로 산마늘 잎 메탄올 추출물은 비만유발 마우스에서 비만개선작용을 나타내었으며, 나아가 인체에서도 유의한 비만개선작용을 할 것으로 생각된다.

The antiobese effects of Allium victorialis (AV) leaf methanol extract were evaluated in a high fat diet (HFD) supplied mice. The changes on the body weight, food consumption, leptin and adiponectin levels as well as the periovarian fat weights and histopathology of adipocytes were examined. The effects were compared with those of a group given 250 mg/kg of metformin. After 91 days of a continuous HFD supply, the mice were significantly showed obesity. However, the obesity induced by the HFD was inhibited by the AV extract treatment at the three different doses (62.5, 125 and 250 mg/kg) respectively. The results suggest that the AV methanol extract is beneficial for improving the diet-induced obesity in humans.

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참고문헌

  1. Ahn YM, Lim SJ, Han HK, Choi SS. Effects of Allium vegetable intake on levels of plasma glucose, lipid and minerals in streptozotocin induced diabetic rats. Korean J Nutrition 2006; 39: 433-443.
  2. Arita Y, Kihara S, Ouchi N, Takahashi M, Maeda K, Miyagawa J, Hotta K, Shimomura I, Nakamura T, Miyaoka K, Kuriyama H, Nishida M, Yamashita S, Okubo K, Matsubara K, Muraguchi M, Ohmoto Y, Funahashi T, Matsuzawa Y. Paradoxical decrease of an adipose-specific protein, adiponectin, in obesity. Biochem Biophys Res Commun 1999; 257: 79-83. https://doi.org/10.1006/bbrc.1999.0255
  3. Bjorbaek, C, Kahn, BB. Leptin signaling in the central nervous system and the periphery. Recent Prog Horm Res 2004; 59: 305-331. https://doi.org/10.1210/rp.59.1.305
  4. DiGirolamo M, Fine JB, Tagra K, Rossmanith R. Qualitative regional differences in adipose tissue growth and cellularity in male Wistar rats fed ad libitum. Am J Physiol 1998; 274: R1460-1467.
  5. Ebihara K, Ogawa Y, Masuzaki H, Shintani M, Miyanaga F, Aizawa-Abe M, Hayashi T, Hosoda K, Inoue G, Yoshimasa Y, Gavrilova O, Reitman ML, Nakao K. Transgenic overexpression of leptin rescues insulin resistance and diabetes in a mouse model of lipoatrophic diabetes. Diabetes 2001; 50: 1440-1448. https://doi.org/10.2337/diabetes.50.6.1440
  6. Fujita H, Fujishima H, Koshimura J, Hosoba M, Yoshioka N, Shimotomai T, Morii T, Narita T, Kakei M, Ito S. Effects of antidiabetic treatment with metformin and insulin on serum and adipose tissue adiponectin levels in db/db mice. Endocr J 2005; 52: 427-433. https://doi.org/10.1507/endocrj.52.427
  7. Hakansson ML, Hulting AL, Meister B. Expression of leptin receptor mRNA in the hypothalamic arcuate nucleus--relationship with NPY neurones. Neuroreport 1996; 7: 3087-3092. https://doi.org/10.1097/00001756-199611250-00059
  8. Inzucchi SE. Oral antihyperglycemic therapy for type 2 diabetes: scientific review. JAMA 2002; 287: 360-372. https://doi.org/10.1001/jama.287.3.360
  9. James PT, Leach R, Kalamara E, Shayeghi M. The worldwide obesity epidemic. Obes Res 2001; 9: 228S-233S. https://doi.org/10.1038/oby.2001.123
  10. Kim TG, Kim SH, Kang SY, Jung KK, Choi DH, Park YB, Ryu JH, Han HM. Antiatherogenic effect of the extract of Allium victorialis on the experimental atherosclerosis in the rabbit and transgenic mouse. Korean J Pharmacog 2000; 31: 149-156.
  11. Ku SK, Kim JW. Hepatoprotective and nephroprotective effects of Allium victorialis leaf extracts on the high fat diet supplied mice. J Vet Clin 2010; 27: 225-231
  12. Lee KT, Choi JH, Kim DH, Son KH, Kim WB, Kwon SH, Park HJ. Constituents and the antitumor principle of Allium victorialis var. platyphyllum. Arch Pharm Res 2001; 24: 44-50. https://doi.org/10.1007/BF02976492
  13. Maebuchi M, Machidori M, Urade R, Ogawa T, Moriyama T. Low resistin levels in adipose tissues and serum in high-fat fed mice and genetically obese mice: development of an ELISA system for quantification of resistin. Arch Biochem Biophys 2003; 416: 164-170. https://doi.org/10.1016/S0003-9861(03)00279-0
  14. Maeda K, Okubo K, Shimomura I, Funahashi T, Matsuzawa Y, Matsubara K. cDNA cloning and expression of a novel adipose specific collagen-like factor, apM1 (AdiPose Most abundant Gene transcript 1). Biochem Biophys Res Commun 1996; 221: 286-289. https://doi.org/10.1006/bbrc.1996.0587
  15. Maeda N, Shimomura I, Kishida K, Nishizawa H, Matsuda M, Nagaretani H, Furuyama N, Kondo H, Takahashi M, Arita Y, Komuro R, Ouchi N, Kihara S, Tochino Y, Okutomi K, Horie M, Takeda S, Aoyama T, Funahashi T, Matsuzawa Y. Diet-induced insulin resistance in mice lacking adiponectin/ACRP30. Nat Med 2002; 8: 731-737. https://doi.org/10.1038/nm724
  16. Mitchell M, Armstrong DT, Robker RL, Norman RJ. Adipokines: implications for female fertility and obesity. Reproduction 2005; 130: 583-597. https://doi.org/10.1530/rep.1.00521
  17. Morange PE, Lijnen HR, Alessi MC, Kopp F, Collen D, Juhan-Vague I. Influence of PAI-1 on adipose tissue growth and metabolic parameters in a murine model of diet-induced obesity. Arterioscler Thromb Vasc Biol 2000; 20: 1150-1154. https://doi.org/10.1161/01.ATV.20.4.1150
  18. Park SH, Ko SK, Chung SH. Euonymus alatus prevents the hyperglycemia and hyperlipidemia induced by high-fat diet in ICR mice. J Ethnopharmacol 2005; 102: 326-335. https://doi.org/10.1016/j.jep.2005.06.041
  19. Sahai A, Malladi P, Pan X, Paul R, Melin-Aldana H, Green RM, Whitington PF. Obese and diabetic db/db mice develop marked liver fibrosis in a model of nonalcoholic steatohepatitis: role of short-form leptin receptors and osteopontin. Am J Physiol Gastrointest Liver Physiol 2004; 287: G1035-1043. https://doi.org/10.1152/ajpgi.00199.2004
  20. Sakaue H, Nishizawa A, Ogawa W, Teshigawara K, Mori T, Takashima Y, Noda T, Kasuga M. Requirement for 3-phosphoinositide-kependent dinase-1 (PDK-1) in insulin-induced glucose uptake in immortalized brown adipocytes. J Biol Chem 2003; 278: 38870-38874. https://doi.org/10.1074/jbc.M306151200
  21. Senthil D, Faulkner JL, Choudhury GG, Abboud HE, Kasinath BS. Angiotensin II inhibits insulin-stimulated phosphorylation of eukaryotic initiation factor 4E-binding protein-1 in proximal tubular epithelial cells. Biochem J 2001; 360: 87-95. https://doi.org/10.1042/0264-6021:3600087
  22. Seufert J, Lubben G, Dietrich K, Bates PC. A comparison of the effects of thiazolidinediones and metformin on metabolic control in patients with type 2 diabetes mellitus. Clin Ther 2004; 26: 805-818. https://doi.org/10.1016/S0149-2918(04)90125-7
  23. Shirataki Y, Motohashi N, Tani S, Sunaga K, Sakagami H, Satoh K, Nakashima H, Kanamoto T, Wolfard K, Molnar J. Antioxidative activity of Allium victorialis L. extracts. Anticancer Res 2001; 21: 3331-3339.
  24. Surwit RS, Kuhn CM, Cochrane C, McCubbin JA, Feinglos MN. Diet-induced type II diabetes in C57BL/6J mice. Diabetes 1988; 37: 1163-1167. https://doi.org/10.2337/diabetes.37.9.1163
  25. Thupari JN, Kim EK, Moran TH, Ronnett GV, Kuhajda FP. Chronic C75 treatment of diet-induced obese mice increases fat oxidation and reduces food intake to reduce adipose mass. Am J Physiol Endocrinol Metab 2004; 287: E97-104. https://doi.org/10.1152/ajpendo.00261.2003
  26. Wolf G, Chen S, Han DC, Ziyadeh FN. Leptin and renal disease. Am J Kidney Dis 2002; 39: 1-11. https://doi.org/10.1053/ajkd.2002.29865
  27. Yamauchi T, Kamon J, Waki H, Imai Y, Shimozawa N, Hioki K, Uchida S, Ito Y, Takakuwa K, Matsui J, Takata M, Eto K, Terauchi Y, Komeda K, Tsunoda M, Murakami K, Ohnishi Y, Naitoh T, Yamamura K, Ueyama Y, Froguel P, Kimura S, Nagai R, Kadowaki T. Globular adiponectin protected ob/ob mice from diabetes and ApoE-deficient mice from atherosclerosis. J Biol Chem 2003; 278: 2461-2468. https://doi.org/10.1074/jbc.M209033200
  28. Yun SN, Moon SJ, Ko SK, Im BO, Chung SH. Wild ginseng prevents the onset of high-fat diet induced hyperglycemia and obesity in ICR mice. Arch Pharm Res 2004; 27: 790-796. https://doi.org/10.1007/BF02980150
  29. Zimmet P. The burden of type 2 diabetes: are we doing enough? Diabetes Metab 2003; 29: S9-18.