• 제목/요약/키워드: 24-steps

검색결과 438건 처리시간 0.02초

재조합 E. coli로부터 발현되는 철단백질의 분리 및 정제

  • 박현규;이지원;김인호
    • 한국생물공학회:학술대회논문집
    • /
    • 한국생물공학회 2001년도 추계학술발표대회
    • /
    • pp.697-700
    • /
    • 2001
  • Iron is an essential nutrient for most organisms, which supplied to them in a protein-iron complex known as ferritin. Ferritins are multimeric proteins found in prokaryotes, plants and animals. They are consisted of spherical shell of 24 subunits defining a cavity of about 8nm in diameter, where an iron core is laid down. Expression of ferritin in recombinant E. coli at $37^{\circ}C$ led to the accumulation of recombinant ferritin. Insoluble form of ferritin was separated from disrupted cells, followed by various primary separation steps with two kinds of buffers. Collected samples from the primary steps were purified by DEAE-cellulose gels packed in a column. The fractions from the DEAE column were assayed to gain the amount and the purity of ferritin by using HPLC and SDS-PAGE.

  • PDF

교과기반평가를 위한 성과중심 교과목 평가설계모형 개발 (Development on the model of outcome-based course evaluation design for Course-Embedded Assessment)

  • 김혜경
    • 공학교육연구
    • /
    • 제18권6호
    • /
    • pp.24-31
    • /
    • 2015
  • The purpose of this study is to develop the model of course evaluation design for CEA(Course-Embedded Assessment), which is focused on course level for assessment reliability. This study was conducted by the process of model development and validation according to Richey & Klein(2007)'s model development methodology. The model of this study was comprised with the three steps of learning objectives based on course outcome, assessment design and goal achievement analysis of course, which were explained by specific design principles. Also, this model was validated by two steps, which one was on the inner validation and the other was the outer validation(Richey & Klein, 2007). Considerations for designing the model of course evaluation have been suggested and a few implications were discussed.

Formation pattern 연구를 통한 AGM 연축전지의 충전 효율 향상 (Improvement of charging efficiency of AGM lead acid battery through formation pattern research)

  • 김성준;손정훈;김봉구;정연길
    • 한국결정성장학회지
    • /
    • 제31권1호
    • /
    • pp.55-62
    • /
    • 2021
  • CO2 가스 발생 감소와 연비향상을 위해서 HEV 차량은 ISG 시스템을 채용하고 있다. 이 ISG 시스템은 배터리가 감당해야 하는 전기 부하를 증대시켰고, 시동 횟수도 급격히 늘어나게 하였다. 이를 위해 AGM 연축전지가 개발되어 사용되고 있으나, 종래의 연축전지에 비해서 formation 중 전해액량 조절이 더 높은 수준으로 유지해야 됨에 따라 충전시간이 약 3배 가량 길어지게 되었다. 본 연구에서는 formation pattern의 최적화를 통해서 충전효율을 증대시켜 충전시간을 단축하고자 하였다. formation pattern의 최적화를 위해서, 16개 multi step에 10개 충전 step과 6개의 방전 step을 적용하고, step별 충전 전류를 조절한 4가지 조건(21 hr, 24hr, 27 hr, 30 hr)으로 시험을 진행하였다. 그 결과 24 hr 시험 조건이 PbO2 변환율이 가장 높게 분석되었고, 용량 103.3 %, 저온시동성능 38 sec, 충전수입성 37.36 A로 나타났다. Multi-step과 방전 step을 적용한 충전 프로그램의 결과, 충전 중에 국부적으로 급격히 발생된 분극화를 제거하고 전류의 손실을 최소함으로써 충전효율을 증가시킬 수 있음을 검증하였다. 이렇게 충전효율을 증가시킴으로써 본 연구에서는 충전시간을 기존에 비해서 약 30 % 감소시키는 탁월한 결과를 얻을 수 있었다.

PAM 인버어터를 이용한 SVC의 출력전압 파형 개선에 관한 연구 (A novel method for improvement of the output voltage waveform of SVC using PAM inverter)

  • 서윤철;모창호;김영민;박현철;유철로
    • 전력전자학회:학술대회논문집
    • /
    • 전력전자학회 1996년도 창립기념 전력전자학술발표회 논문집
    • /
    • pp.67-70
    • /
    • 1996
  • This paper presents SVC which use PAM method and eliminate harmonics. Inverter is connected directly so that SVC improve output voltage waveform into 24 steps. Inverter output waveform THD is reduced to 6.89%. Leading control of reactive power generated in power system is possible. Snubber is added to reduce switching loss.

  • PDF

국내산 다이아스포아의 열분해에 관한 연구 (Study on Thermal Decomposition of Korean Diaspore)

  • 이헌수;손명모;박희찬
    • 한국세라믹학회지
    • /
    • 제24권4호
    • /
    • pp.307-312
    • /
    • 1987
  • The investigation has been performed to study thermal dehydration of Song-sug, Pyung-il-do and Chung-mu diapore in Korea. Thermal analysis of Korean diaspore showed two steps of dehydration by dispore and kaolinite. The activation energy of dehydration reation of each diaspore was calculated by kissinger's method, and the results obtained were 63.608, 37.867 and 54.885Kcal/mol, respectively.

  • PDF

Individual Strategies for Problem Solving

  • Revathy Parameswaran
    • 한국수학교육학회지시리즈D:수학교육연구
    • /
    • 제9권1호
    • /
    • pp.11-24
    • /
    • 2005
  • Problem solving is an important aspect of learning mathematics and has been extensively researched into by mathematics educators. In this paper we analyze the difficulties students encounter in various steps involved in solving problems involving physical and geometrical applications of mathematical concepts. Our research shows that, generally students, in spite of possessing adequate theoretical knowledge, have difficulties in identifying the hidden data present in the problems which are crucial links to their successful resolutions. Our research also shows that students have difficulties in solving problems involving constructions and use of symmetry.

  • PDF

순환성 코드를 사용한 Majority logic 디코딩 (The majority-logic decoding for cyclic codes)

  • 강창언;정연호
    • 한국통신학회:학술대회논문집
    • /
    • 한국통신학회 1984년도 춘계학술발표회논문집
    • /
    • pp.22-24
    • /
    • 1984
  • In this paper, the (15,7) cylic codesused EG(2,2) were decoded by one step majority logic decoding. This decoding algorithm is based on the properties of finite geometries and can be simply implemented for moderate length n. especially one step majority logic decoding is attractive because the complexity and the cost of the majority logic decoder increase very rapidly with L, the number of decoding steps. The theorectical and experimental results show that the majority logic decoding presented in this paper is a relatively effective decoding scheme.

  • PDF

유연생산시스템의 최적구성 결정 (A Determination on Optimal Configuration of Flexible Manufacturing System)

  • 장진익;김원중
    • 산업경영시스템학회지
    • /
    • 제24권67호
    • /
    • pp.19-26
    • /
    • 2001
  • A design issue for a flexible manufacturing system is to find the number of each resource type which assures a given production ratio with a minimal cost. The FMS is modeled as a closed queueing network with a local buffer of limited capacity. An efficient method to determine this optical configuration is presented. The proposed method consists of three steps : 1) determine a lower configuration, 2) derive a heuristic solution, 3) find the optimal solution. The derived heuristic solutions are close to the optical solution.

  • PDF

정상인의 경사로 보행 시 경사각에 따른 시공간적 보행 특성 분석 (The Spatio-temporal Analysis of Gait Characteristics during Ramp Ascent and Descent at Different Inclinations)

  • 한진태;조정선;배성수
    • The Journal of Korean Physical Therapy
    • /
    • 제18권1호
    • /
    • pp.95-106
    • /
    • 2006
  • Purpose: The aim of this study was to investigate the kinematics of young adults during ramp climbing at different inclinations. Methods: Twenty-three subjects ascended and descended four steps at four different inclinations(level, $8^{\circ},\;16^{\circ},\;24^{\circ}$). Temporal-spatial parameters were measured by GaitRite system(standard mat). Groups difference was analysed with on-way ANOVA and Student-Newman-Keuls test. Results: The different kinematics of ramp ascent and descent were analysed and compared to level walking patterns. In ascending ramps, step length and stride length decreased with $24^{\circ}$ inclination (p<.000). Stance duration increased with $24^{\circ}$, but swing duration decreased with $24^{\circ}$ inclination (p<.000). Step time and velocity decreased with $16^{\circ}C,\;24^{\circ}$ inclination (p<.000). Cadence decreased with all inclination($8^{\circ},\;16^{\circ},\;24^{\circ}$)(p<.000). In descending ramps, step length and stride length, velocity decreased with all inclination($8^{\circ},\;16^{\circ},\;-24^{\circ}$)(p<.000). Stance duration increased with all inclination($8^{\circ},\;-16^{\circ},\;-24^{\circ}$) and swing duration decreased with all inclination($-8^{\circ},\;-16^{\circ},\;-24^{\circ}$)(p<.000). But Step time was not differentiated with different inclinations. Cadence decreased with only. $8^{\circ}$ inclination(p<.05). Conclusion: These results suggest that there is a certain inclination angle or angular range where subjects do switch between level walking and ramp walking gait pattern. This shows their motor control strategy between level and ramp walking. Further studies are necessary to confirm and detect the ascent and descent ramp gait patterns.

  • PDF

Convenient Synthesis of Optically Pure α-Mono and α,α-Disubstituted β-Amino Acids

  • Lee, Hyun-Soo;Park, Jung-Dae;Kim, Dong-H.
    • Bulletin of the Korean Chemical Society
    • /
    • 제24권4호
    • /
    • pp.467-472
    • /
    • 2003
  • Optically pure α-mono- and α, α-disubstituted β-amino acids were conveniently prepared in four steps and in 27-40% overall yields from the correspondingly substituted racemic β-hydroxy acids that can be readily obtained from diethyl malonate. In the synthesis, (S)-phenylethylamine has been used as a resolving agent and as a source of the amino group in the β-amino acids.