• Title/Summary/Keyword: 20M10

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Adsorptive Stripping Voltammetric Determination of Sex Hormones in Medicines (의약물 중 성호르몬의 흡착벗김 전압-전류법 정량)

  • Kim, Il Kwang;Chun, Hyun Ja;Jang, Kyung Seon
    • Analytical Science and Technology
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    • v.7 no.2
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    • pp.133-140
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    • 1994
  • The determination of sex hormones by adsorpive stripping voltammetry in $1.0{\times}10^{-2}M$ sodium hydroxide as supporting electrolyte has been investigated in the conditions as follows : 240sec. for deposition time, -0.80 volts deposition potential, medium size mercury drop, and 20mV/sec scan rate. Calibration curve has shown a linearlity in the range of $5.0{\times}10^{-9}M$ to $8.0{\times}10^{-7}M$ and the detection limits have been $8.0{\times}10^{-10}M$ for progesterone and $1.4{\times}10^{-9}M$ for testosterone propionate. This method has shown such a good sensitivity even in dilute solution that has been use full for analyzing sex hormones in medical supplies without interference of additives.

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Distribution of Biomass and Production of Robinia pseudoacacia Plantation in Korea (아까시나무조림지(造林地)의 물질생산량(物質生産量)에 관(關)한 연구(研究))

  • Kim, Kap Duk;Kim, Tae Wook;Lee, Kyong Jae;Kim, Joon Seon
    • Journal of Korean Society of Forest Science
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    • v.68 no.1
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    • pp.60-68
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    • 1985
  • To study the comparison of the aboveground biomass of Robinia pseudoacacia L. of 8-, 13- and 20-year-old plantations, the experimental plot of $100m^2$ in size located in Youngin-gun were selected. Seven sample trees at different stand ages selected taking account of DBH distribution were felled and the diagram of oven-dry weight distribution of stem, branch and leaf for each 1m segment was constructed. The dry weight of each part of plots was estimated by the method of basal area ratio. If the estimations are extended to a hectare area stand, it contains 36.72, 69.28 and 118.67 tons of the aboveground standing crops in 8-, 13- and 20- year-old stand respectively. The net production was estimated to be as much as 12.56, 13.23 and 16.78 tons per hectare per year and the net assimilation ratio 3.66, 4.13 and 2.50 kg/kg/yr in 8-, 13- and 20-year-old stand respectively. And the efficiency of leaves to produce stem was 1.69, 2.10 and 0.96 kg/kg/yr in same order.

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Effects of Planting Density on Plant Growth and Tuber Yield of Rehmannia glutinosa (지황(地黃) 재식밀도(栽植密度)가 생육(生育) 및 수량(收量)에 미치는 영향(影響))

  • Choi, In-Sik;Song, In-Kyu;Kim, Jun-Ho;Cho, Jin-Tae;Hong, Yu-Ki;Park, Song-Kyu;Park, Jong-Kooi
    • Korean Journal of Medicinal Crop Science
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    • v.1 no.1
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    • pp.70-73
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    • 1993
  • This experiment was carried out to investigate the effect of planting density on thegrowth and yield of Rehmannia glutinosa. A local variety was planted on the 100cm row, with different planting density of 20, 30 and 40 plants per $m^2$. Nitrogen, phosporus and potassium fertilizers were applied as 12, 12, and 16 kg /10a, respectively with compost of 1,000kg /10a. Plant height became shorter with increase in the planting density from 20 to 40 $plants\;/\;m^2$. But emergence date, leaf length and width were not significantly changed with planting density. As the planting density was increased, tuber diameter got slender and tuber number was decreased, but tuber length was not affected. The tuber yield was 793kg /10a at the planting density of 20 $plants\;/\;m^2$ and it increased 40% at 30 $plants\;/\;m^2$ and 45% at 40 $plants\;/\;m^2$ Regarding on the farmer's income, optimum planting density was estimated 30 $plants\;/\;m^2$.

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Induction of Valiant of Cyrtomium caryoptideum var. coreanum Nakai by Chemical Mutagenesis In vitro and RAPD Analysis (기내에서 화학돌연변이원 처리에 의한 참쇠고비의 변이주 유기 및 RAPD 분석)

  • Jeong Jin-A;Lee Cheol-Hee
    • Korean Journal of Plant Resources
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    • v.19 no.2
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    • pp.374-380
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    • 2006
  • With the aim of inducing mutation in fern Cyrtomium caryoptideum var. coreanum, rhizome segments of In vitro-grown cultures were treated with chemical mutagens such as EMS, NMU and colchicine. Based on regeneration ratios, sensitivities for each treatments were assessed and also optimum treatment condition of each mutagens was explored. Optimum concentration for EMS treatment was considered to be 20 to 50mM and for NMU 5 to 10mM. NMU was found to be more effective in inducing chlorophyll and morphological variations than EMS. The RAPD were performed to check the genetic modification of phenotypical variants. As a result, polymorphic DNA band patterns between wild type and variants were observed by two 10-mer primers.

Particle Loss Reduction Technique Using Dielectrophoresis in Microfluidic Channel (유전영동을 이용한 미세유체채널 내부의 입자 손실 저감 기술)

  • Kang, Dong-Hyun;Kim, Min-Gu;Kim, Yong-Jun
    • Journal of Sensor Science and Technology
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    • v.20 no.5
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    • pp.357-362
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    • 2011
  • This paper demonstrates a novel electrodynamic technique to remove particles from the wall of microchannels. Dielectrohporesis(DEP) is generated by applying alternating electric potentials to the interdigitated electrodes integrated at the bottom of the micro-channel. The proposed technique is applied to a general microfluidic channel as a feasibility test. To examine the wall loss reduction efficiency, 10 ${\mu}m$ diameter Polystyrene latexes(PSL) were supplied to the inlet of the device. Then, the concentration of collected particles through devices was measured. In the experiment for 10 ${\mu}m$ diameter PSL particles, the concentration of the injected particles was $174.25{\times}10^4$ particles/ml. However, the concentration of collected particles at the outlet was $52.25{\times}10^4$ particles/ml. Only 30 % of particles had arrived at the outlet and 70 % of particles had adhered to the wall of the microfluidic channel. By applying alternating electric potentials from 0 to 20 $V_{pp}$ at 3 MHz, the concentration of injected particles was 135.00${\times}10^4$ particles/ml, the concentration of collected particles was increased as $105.25{\times}10^4$ particles/ml at 20 $V_{pp}$ at the outlet. When the electric potential was 20 $V_{pp}$, the particle loss was decreased by 39 % (initial loss: 70 %, loss at 20 Vpp: 31 %) with 10 ${\mu}m$ particle. The particle loss was decreased along to the incensement of electric potentials and the enlargement of the diameter of particles. According to these measured results, it was confirmed that the proposal of using DEP technique could be a good candidate for particle loss reduction in micro-particle processing chip application. Moreover, it is expected that the proposed technique could enhance performance of microfluidic and biochip devices.

Fruit Soft Rot of Sweet Persimmon Caused by Mucor piriformis in Korea

  • Kwon, Jin-Hyeuk;Ahn, Gwang-Hwan;Park, Chang-Seuk
    • Mycobiology
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    • v.32 no.2
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    • pp.98-101
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    • 2004
  • A fruit soft rot caused by Mucor piriformis occurred on sweet persimmon storages in Jinju, Changwon and Gimhae, Gyeongnam province, Korea, 2003. The disease infection usually started from wounding after cracking of fruits. At first, the lesions started with water soaked and rapidly softened and diseased lesion gradually expanded. Colonies on potato dextrose agar at $20^{\circ}C$ were whitish to olivaceous-buff Sporangia were globose, black and $96{\sim}153{\mu}m$ in size. Sporangiophores were $26{\sim}42{\mu}m$ in width. Sporangiospores were ellipsoid and $5.8{\sim}10.6{\times}4.3{\sim}7.6{\mu}m$ in size. Columella was obovoid, cylindrical-ellipsoidal, pyriform, subglobose and $80{\sim}125{\mu}m$ in size. Optimal temperature for mycelial growth was $20^{\circ}C$ on PDA. The causal organism was identified as M. piriformis. This is the first report of fruit soft rot on sweet persimmon caused by M. piriformis in Korea.

Agricultural Radial Collector Wells in South Korea and Sustainability (한국의 농업용 방사상 집수정 현황 및 지속가능성)

  • Hong, Soun-Ouk;Song, Sung-Ho;An, Jung-Gi;Kim, Jin-Sung
    • The Journal of Engineering Geology
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    • v.26 no.3
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    • pp.331-337
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    • 2016
  • Radial collector wells (RCWs) have been managed by Korea Rural Community Corporation (KRC) since 1983, installing 98 wells for agriculture in rural area over the country. Among them, 20 wells were installed upstream of 5 subsurface dams and the remaining were installed regardless of the subsurface dam. Most of RCWs have been developed in 1980s and 1990s, and 83 wells have been passed more than 20 years after construction. The number of horizontal arms for RCWs varies from 9 to 28, with length and diameter being 10~30 m and 65 mm, respectively. The central caisson with an inner diameter of 3.5 m was commonly constructed to a depth of 10 m. The maximum pumping rates in RCWs, which are located at distances of 10 to 1,200 m from the river, are 2,000~10,000 m3/day. RCW has a fundamental problem that reduced pumping capacity and degraded well efficiency, due to the physical and chemical clogging. From the feasibility test for improving RCW performance, specific capacity increased to 67% after rehabilitation. TV logging for RCW horizontal arm shows that near the caisson is more severe clogging. From the results of this study, KRC has established the guidebook for monitoring and improving well efficiency through physical/chemical treatment, well logging, and hydraulic tests and managed RCWs periodically with its rehabilitation methods.

Note on the New Record of Mitrula paludosa (Geoglossaceae) in Korea (콩나물버섯과 미기록종 Mitrula paludosa (습지등불버섯 : 신칭)의 보고)

  • Park, Yong-Woo;Koo, Chang-Duck;Hong, Dae-Eui;Kim, Tae-Heon
    • The Korean Journal of Mycology
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    • v.37 no.1
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    • pp.104-107
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    • 2009
  • Mitrula paludosa (Geoglossaccae, Helotiales, Ascomycetes) is reported about morphological and ecological characteristics for the first time in Korea. The mushroom fruited on rotten pine leaves or fine twigs in ditches with flowing or standing water in Sogri Mountain National Park from May to June. The apothecium of M. paludosa is 20 to 50 mm tall. The cap is bright yellow to orange, cylindrical to wrinkled ovoid and $5{\sim}10\;{\times}\;10{\sim}20$ mm. The stem is whitish and cylindrical distinctly set off from the cap. The ascospore is hyaline, smooth long elliptical fusoid with round ends and $13{\sim}17\;{\times}\;1.5{\sim}2.0{\mu}m$. The ascus is unitunicate, $90{\sim}110\;{\times}\;5{\sim}6{\mu}m$, and has eight spores biseriately lined to the inoperculate apex. Paraphyses are septate filiform, $95{\sim}120\;{\times}\;1.0{\sim}1.5{\mu}m$, $7{\sim}10{\mu}m$ longer than the asci.

Comparative Study of Tritium Analysis Method with High-Volume Counting Vial

  • Yoon, Yoon Yeol;Kim, Yongcheol
    • Journal of Radiation Protection and Research
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    • v.45 no.3
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    • pp.142-146
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    • 2020
  • Background: Tritium (3H) analysis in groundwater was difficult because of its low activity. Therefore, the electrolytic enrichment method was used. To improve the detection limit and for performing simple analysis, a high-volume counting vial with the available liquid scintillation counter (LSC) was investigated. Further, it was compared with a conventional 20-mL counting vial. Materials and Methods: The LSC with the electrolytic enrichment method was used 3H analysis in groundwater. A high-volume 145-mL counting vial was compared with a conventional 20-mL counting vial to determine the counting characteristics of different LSCs. Results and Discussion: When a Quantulus LSC was used, the counting window between channels 35 and 250 was used. The background count was approximately 1.86 cpm, and the counting efficiency increased from 8% to 40% depending on the mixing ratio of the volume of sample and cocktail solution. For LSC-LB7, the optimum counting window was between 1 and 4.9 keV, which was selected by the factory (Hitachi Aloka Medical Ltd., Japan) by considering quenching using a standard external gamma source. The background count of LSC-LB7 was approximately 3.60 ± 0.29 cpm when the 145-mL vial was used and 2.22 ± 0.17 cpm when the 20-mL vial was used. The minimum detectable activity (MDA) of the 20-mL vial was greater for LSC-LB7 than for Quantulus. The MDA with the 145-mL vial was improved to 0.3 Bq/L when compared with the value of 1.6 Bq/L for the 20-mL vial. Conclusion: The counting efficiency when using the 145-mL vial was 27%, whereas it was 18% when using the 20-mL vial. This difference can be attributed to the vial volume. The figure of merit (FOM) of the 145-mL vial was four times greater than that of the 20-mL vial because the volume of the former vial is approximately seven times greater than that of the latter. Further, the MDA for 3H decreased from 1.6 to 0.3 Bq/L. The counting efficiency and FOM of LSC-LB7 was slightly less than those of Quantulus when the 20-mL vial was used. The background counting rate of the Quantulus was lower than that of the LSC-LB7.

Comparison of Central and Peripheral Refraction in Myopic Eyes after Corneal Refractive Surgery and Emmetropes (굴절교정수술을 받은 근시안과 정시안에서 중심부 및 주변부의 굴절력 비교)

  • Kim, Jeong-Mee;Lee, Koon-Ja
    • Journal of Korean Ophthalmic Optics Society
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    • v.20 no.2
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    • pp.157-165
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    • 2015
  • Purpose: To evaluate changes in central and peripheral refraction along the horizontal visual fields in myopic corneal refractive surgery group compared with emmetropes. Methods: One hundred twenty eyes of 60 subjects ($23.56{\pm}2.54$ years, range: 20 to 29) who underwent myopic refractive surgery and 40 eyes of 20 emmetropes ($22.50{\pm}1.74$ years, range: 20 to 25) were enrolled. The central and peripheral refractions were measured along the horizontal meridianat $5^{\circ}$, $10^{\circ}$, $15^{\circ}$, $20^{\circ}$, $25^{\circ}$ in the nasal and temporal areas using an open-field autorefractor. For analysis of post-op group, the group was classified by pre-op spherical equivalents of < -6.00 D and ${\geq}-6.00D$ as two post-op groups. Results: Pre-op spherical equivalent was $-4.56{\pm}0.92D$ (rang: -2.50 to -5.58 D) in post-op group 1, and $-7.09{\pm}0.96D$ (rang: -6.00 to -9.00 D) in post-op group 2. Spherical equivalent (M) in the emmetropes ranged from $-0.20{\pm}0.22D$ at center to $-0.64{\pm}0.83D$ at $25^{\circ}$ in the temporal visual field and to $-0.20{\pm}0.67D$ at $25^{\circ}$ in the nasal visual field; M in post-op group 1 ranged from $-0.16{\pm}0.29D$ at center to $-5.29{\pm}1.82D$ at $25^{\circ}$ in the temporal visual field and to $-4.48{\pm}1.88D$ at $25^{\circ}$ in the nasal visual field; M in post-op group 2 ranged from $-0.20{\pm}0.32D$ at center to $-7.98{\pm}2.08D$ at $25^{\circ}$ in the temporal visual field and to $-7.90{\pm}2.26D$ at $25^{\circ}$ in the nasal visual field. Among the three groups, there was no significant difference in M at central visual field (p=0.600) and at $5^{\circ}$ in the temporal visual field (p=0.647), whereas, there was significant difference in M at paracentral and peripheral visual field (p=0.000). Conclusions: Emmetropes had relatively constant refractive errors throughout the central and peripheral visual field and showed myopic peripheral defocus along the horizontal visual field. On the other hand, in myopic corneal refractive surgery group, there were significant differences in refractive errors between the central and peripheral visual field compared with differences in the central and peripheral refraction patterns of emmetropes.