• 제목/요약/키워드: 2-probe method

검색결과 759건 처리시간 0.032초

Molecular Identification of the Toxic Alexandrium tamiyavanichii (Dinophyceae) by the Whole-cell FISH Method

  • Kim Choong-Jae;Yoshimatsu Sada-Akfi;Sako Yoshihiko;Kim Chang-Hoon
    • Fisheries and Aquatic Sciences
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    • 제7권4호
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    • pp.175-183
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    • 2004
  • The dinoflagellate Alexandrium tamiyavanichii Balech, a producer of toxins causing paralytic shellfish poisoning (PSP), has recently been considered as one of main organisms responsible for toxication of shellfish in Japan. In this study, A. tamiyavanichii was subjected to a molecular phylogenetic analysis inferred from 28S rDNA D1-D2 sequences and a species-specific LSU rRNA-targeted oligonucleotide DNA probe was designed to identify A. tamiyavanichii using the whole cell-FISH (fluorescence in situ hybridization). The sequences of the 28S rDNA D1-D2 region of A. tamiyavanichii showed no difference from A. cohorticular AF1746l4 (present name A. tamiyavanichii) and formed a distinct clade from the 'tamarensis species complex'. The probe, TAMID2, reacted specifically with A. tamiyavanichii cultured cells, without any cross-reaction with other species belonging to the same genus, including A. tamarense, A. catenella, A. affine, A. fraterculus, A. insuetum and A. pseudogonyaulax. In a test of cross-reactivity with a field sample, TAMID2 reacted consistently with only A. tamiyavanichii, indicating that the present protocol involving the TAMID2 probe might be useful for detecting toxic A. tamiyavanichii in a simple and rapid manner.

동축선로 프로브를 이용한 프린트 배선 회로용 기판 재료의 주파수 및 온도 변화에 따른 유전특성 연구 (A Study on Dielectric Properties of Printed Circuit Board(PCB) Materials with Variation of Frequency and Temperature using Coaxial Air Line Probe)

  • 박종성;김종헌
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 1998년도 추계학술대회 논문집
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    • pp.187-190
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    • 1998
  • In this paper a probe for the' measurement of dielectric properties of dielectric sheet materials is designed and implemented as a coaxial air line type. Using the broadband impedance method with this measurement probe the dielectric constant and loss tangent of the glass-epoxy and teflon are determined in the frequency range of 0.1 - l.O[GHz] with the temperature variation from $25[^{\circ}C]$ up to $65[^{\circ}C]$. A measured relative dielectric constant of the glass-epoxy is 4.42 and a loss tangents is 0.019 relatively, and the relative dielectric constants of teflon is 2.17 and a loss tangents is 0.002 relatively

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저 Reynolds 수 영역에서 Reynolds 수가 5공 프로우브의 보정에 미치는 영향 (The Effect of Reynolds Number on the Calibration of a Five-Hole Probe at Low Reynolds Numbers)

  • 이상우;전상배
    • 유체기계공업학회:학술대회논문집
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    • 유체기계공업학회 2000년도 유체기계 연구개발 발표회 논문집
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    • pp.193-199
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    • 2000
  • The effects of Reynolds number on the non-nulling calibration of a cone-type five-hole probe in low-speed flows have been investigated at the Reynolds numbers of $2.04{\times}10^3,\;4.09{\times}10^3$, and $6.13{\times}10^3$. The calibration is conducted at the pitch and yaw angles in ranges between -35 degrees and 35 degrees with an angle interval of 5 degrees. The result shows that each calibration coefficient, in general, is a function of the pitch and yaw angles, so that the pre-existing calibration data in a nulling mode are not enough in accounting for the full non-nulling calibration characteristics. Due to the interference of the probe stem, the calibration coefficients have more Reynolds number sensitivity at positive pitch angles than at negative ones.

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Coated conductor에서 magneto-optical image와 scanning hall probe를 이용한 flux profile의 측정 및 분석 (Measurement and Analysis of the Flux Profiles of the Coated Conductors using Magneto-optical Image and Scanning Hall Probe)

  • 이효연;곽기성;이준규;유재은;염도준
    • Progress in Superconductivity
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    • 제11권2호
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    • pp.128-134
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    • 2010
  • The magnetic flux profiles in SmBCO and YBCO coated conductors(CC) in the presence of the external field were comparatively investigated by magneto-optic image and scanning hall probe measurements. The current distributions calculated by using the inversion method from measured field profiles show that the decrease of current densities near the edges of SmBCO CC is more significant than those of YBCO CC. Through the comparison of the numerical analysis based on Kim's critical state model and the Brandt and Indenbom's solution, we found that this feature is related to their different field dependant properties of the critical current densities.

초정밀 안내면 레일의 평행도 및 진직도 동시측정 (Parallelism and Straightness Measurement of a Pair of Rails for Ultra Precision Guide-ways)

  • 황주호;박천흥;;김승우
    • 한국정밀공학회지
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    • 제24권3호
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    • pp.117-123
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    • 2007
  • This paper describes a three-probe system that can be used to measure the parallelism and straightness of a pair of rails simultaneously. The parallelism is measured using a modified reversal method, while the straightness is measured using a sequential two-point method. The measurement algorithms were analyzed numerically using a pair of functionally defined rails to validate the three-probe system. Tests were also performed on a pair of straightedge rails with a length of 250 mm and a maximum straightness deviation of $0.05{\mu}m$, as certified by the supplier. The experimental results demonstrated that the parallelism-measurement algorithm had a cancellation effect on the probe stage motion error. They also confirmed that the proposed system could measure the slope of a pair of rails about $0.06{\mu}rad$. Therefore, by combining this technique with a sequential differential method to measure the straightness of the rails simultaneously, the surface profiles could be determined accurately and eliminate the stage error. The measured straightness deviation of each straight edge was less than $0.05{\mu}m$, consistent with the certified value.

Scanning Hall probe를 이용한 coated conductor의 field profile 측정과 current profile 계산 (Measurements of the field profiles using scanning Hall probe and calculation of the current profiles of coated conductors)

  • 유재은;이상무;정예현;이재영;정용환;염도준;김호섭;하홍수;오상수
    • Progress in Superconductivity
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    • 제8권2호
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    • pp.169-174
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    • 2007
  • We measured the field profiles, H(x)'s of coated conductors by using scanning Hall probe method when various magnetic fields, $H{_\alpha}'s$ or currents, I's were applied. From the measured field profiles, we calculated the current profiles, J(x)'s by the inversion method. The calculated J(x)'s of coated conductors show some different properties from the standard critical state model. $J{_c}'s$ are inhomogeneous varying with the positions and are not constant when $H_{\alpha}$ or I changes. And when I decreases the features of current reversion are remarkably different from the model.

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Real-Time Plasma Process Monitoring with Impedance Analysis and Optical Emission Spectroscopy

  • Jang, Hae-Gyu;Kim, Dae-Kyoung;Kim, Hoon-Bae;Han, Sa-Rum;Chae, Hee-Yeop
    • 한국진공학회:학술대회논문집
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    • 한국진공학회 2009년도 제38회 동계학술대회 초록집
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    • pp.473-473
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    • 2010
  • Plasma is widely used in various commercial etchers and chemical vapor deposition. Unfortunately, real-time plasma process monitoring is still difficult. Some methods of plasma diagnosis is improved, however, it is possible for real-time plasma diagnosis to use non-intrusive probe only. In this research, the object is to investigate the suitability of using impedance analysis and optical emission spectroscopy (OES) for real-time plasma process monitoring. It is assumed that plasma system is a equivalent circuit. Therefore, V-I probe is used for measuring impedance, which can be a new non-intrusive probe for plasma diagnosis. From impedance data, we tried to analyse physical properties of plasma. And OES, the other method of plasma diagnosis, is a typical non-intrusive probe for analyzing chemical properties. The amount of the OES data is typically large, so this poses a difficulty in extracting relevant information. To solve this problem, principal component analysis (PCA) can be used. For fundamental information, Ar plasma and $O_2$ plasma are used in this experiment. This method can be applied to real-time endpoint and fault detections.

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상완혈 자침시 복부 장기의 안전성 확보를 위한 초음파시스템의 신뢰도 측정 (Reliability Measurement of the Ultrasound for Safety of the Abdominal Organs on Acupuncturing Sangwan (CV13))

  • 김지혜;백태현
    • 대한한의진단학회지
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    • 제15권2호
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    • pp.203-214
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    • 2011
  • Objectives: The objective of the current study is to determine whether an ultrasound device system is adequate for measuring distance from the abdominal surface of Sangwan (CV13) to peritoneum in front of anterior surface of liver. Methods: We recruited 3 healthy young male subjects and 2 sonographers. The each sonographer measured vertical shortest distance from the abdominal surface of Sangwan (CV13) to peritoneum in front of anterior surface of liver with a ultrasound device with three methods of 3.5 MHz convex probe and gel, 9 MHz linear probe and gel, and 9 MHz linear probe and solid gel pad, three times in random order. Because the total variation could be divided into repeatability, reproducibility and subject-to-subject variation in Gage R&R method, we compared the sources of variation associated with the measurement system with an analysis of variance model. Results & Conclusions: Number of distinct categories is calculated on the basis of standard deviation of subject-to-subject divided by standard deviation of total Gage R&R. If the number of categories is five or more, the measurement system may be acceptable for the analysis of the process. The number of distinct categories of the ultrasound device system for measuring distance from the abdominal surface of Sangwan (CV13) to peritoneum in front of anterior surface of liver were 9.20 (9 MHz linear probe and gel), 14.70 (9 MHz linear probe and solid gel pad). So we concluded that repeatabilities and reproducibilities of the ultrasound device system for measuring distance from the abdominal surface of Sangwan (CV13) to peritoneum in front of anterior surface of liver with the methods of 9 MHz linear probe and gel, and 9 MHz linear probe and solid gel pad were acceptable.

원자간격 현미경의 캔틸레버의 정량적 특성평가를 위한 계측 시스템 개발 (Development of Measurement System for Quantitative Measurement of Cantilever in Atomic Force Microscopy)

  • 권현규;남기호
    • 한국기계가공학회지
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    • 제6권2호
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    • pp.22-27
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    • 2007
  • In this study, the two methods of stiffness measurement(Spring constant) of cantilever were proposed for quantitative measurement in Atomic Force Microscopy(AFM). As the 1st method for the measurement of stiffness, the probe method, which is used in the measurement of the semiconductor mechanical and electrical properties, was applied to the measurement of the cantilever. Experiments by the probe method were performed finding the resistance value of cantilever. As the results, the resistance was measured differently along with the dimension and the thickness of cantilever that determined the stiffness(spring constant) of the lever. As the 2nd method, the vibration characteristics(Dunkerley expression) is used to obtain the stiffness of the complex structure which is combined by AFM cantilever and the standard cantilever. We measured the resonant frequency from the complex structure using the micro stages and stereo microscope. As the results, we confirmed that the vibration characteristics(Dunkerley expression) is effected the micro complex structure of AFM cantilever.

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원호검사법을 이용한 진원도 측정 방법의 개선에 관한 연구 (A Study on the Improvement of Circularity Measurement Using Circular Test Method)

  • 이승수;김민주;전언찬
    • 한국공작기계학회논문집
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    • 제11권2호
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    • pp.24-29
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    • 2002
  • A study for the circularity of machine tool is classified into two ways. One is to measure the circularity of machine tool by a existing measuring device. The other is to measure the circularity by remodeling the existing measuring device. We will improve on measurement of circularity using circular test method by a two-dimensional probe and a master ring for machining tools and investigate influence of trigger method, acceleration data sampling.