• Journal of Microbiology and Biotechnology
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• v.14 no.3
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• pp.584-592
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• 2004

A thermostable $\beta$-glycosidase gene, tfi $\beta$-gly, was cloned from the genomic library of Thermus filiformis Wai33 A1. ifi $\beta$-gly consists of 1,296 bp nucleotide sequence and encodes a polypeptide of 431 amino acids. It shares a strong amino acid sequence similarity with the $\beta$-glycosidases from other Thermus spp. belonging to the glycosyl hydrolase family 1. In the present study, the enzyme was overexpressed in Escherichia coli BL21 (DE3) using the pET21b(+) vector system. The recombinant enzyme was purified to homogeneity by heat treatment and a $Ni^{2+}$-affinity chromatography. Polyacrylamide gel electrophoresis (PAGE) showed that the recombinant Tfi $\beta$-glycosidase was a monomeric form with molecular mass of 49 kDa. The temperature and pH range for optimal activity of the purified enzyme were 80- $90^{\circ}C$ and 5.0-6.0, respectively. Ninety-three percent of the enzyme activity was remained at $70^{\circ}C$ after 12 h, and its half-life at $80^{\circ}C$ was 6 h, indicating that Tfi $\beta$-glycosidase is highly thermostable. Based on its K_m$, or $K_{cat}K_m$, ratio, Tfi $\beta$-glycosidase appeared to have higher affinity for $\beta$-D-glucoside than for $\beta$-D-galactoside, however, $K_{cat} for \beta$-D-galactoside was much higher than that for $\beta$-D-glucoside. The activity for lactose hydrolysis was proportionally increased at $70^{\circ}C$ and pH 7.0 without substrate inhibition until reaching 250 mM lactose concentration. The specific activity of Tfi TEX>$\beta$-glycosidase on 138 mM lactose at $70{^\circ}C$ and pH 7.0 was 134.9 U/mg. Consequently, this newly cloned enzyme appears to have a valuable advantage of conducting biotechnological processes at elevated temperature during milk pasteurization in the production of low-lactose milk.

• Korean Journal of Food Science and Technology
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• v.41 no.1
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• pp.27-31
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• 2009

Top spray-drying method is frequently utilized for flavor encapsulation, but the top spray-dried products frequently suffer from high losses of volatile flavor as the result of a high processing temperature (150-$300^{\circ}C$). In an effort to solve these problems, a low-temperature fluidized-bed granulating method was utilized to encapsulate the flavor. For the encapsulation of sesame oil, oil-in-water emulsions of sesame oil and a mixture of maltodextrin, modified starch, gum arabic, and gellan gum were bottom-sprayed at milder temperatures (70-$100^{\circ}C$) using a fluidized-bed granulator. Sesame oil extracts from microcapsules were obtained via a simultaneous distillation/extraction technique, and the retention of volatile flavor compounds was analyzed via a gas chromatography-mass spectrometry. The retention of volatile flavors of sesame oil per se, spray-dried and fluidized-bed granulated microcapsules after 3-day-storage at $37^{\circ}C$ were 0.8%, 37.2%, and 42.0%, respectively. In addition, the low-temperature fluidized-bed granulation showed higher encapsulation yield and sensory preferences for the application of commercial products (beef rice porridge), as compared to spray drying.

• The Journal of Engineering Geology
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• v.16 no.4 s.50
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• pp.337-349
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• 2006

Seven hundred and fifty one fractures of the rhyolitic tuffaceous rock masses were mapped using 6 scanlines placed on rock slope exposures that were within 8.02 km of Busan-Ulsan highway. These data were analyzed to find the number of fracture sets that exist in the rock slopes and the probability distributions of orientation, spacing, trace length and fracture size in 3-D for each of the fracture sets. All the fracture set orientation distributions exhibit high variability. The Fisher distributions were found to be unsuitable to represent the statistical distribution of orientation for most of the fracture sets. The probability distributions, gamma, exponential and lognormal were found to be highly suitable to represent the distribution of spacing and semi-trace length of fracture sets. In obtain-ing these distributions, corrections were applied for sampling biases associated with spacing and trace length. The generated fracture system in 3-D was used to make predictions of fracture traces for each fracture set on 2-D win-dows. Developed stochastic 3-D fracture network for the rock mass was validated by comparing statistical proper-ties of the observed fracture traces on scanlines with the predicted fracture traces on the scanlines. This exercise fumed out to be successful.

• Journal of Korean Society of Steel Construction
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• v.10 no.1 s.34
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• pp.47-61
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• 1998

The behavior of horizontally curved I-girder bridges is complex because the flexural and torsional behavior of curved girders are coupled due to their initial curvature. Also, the behavior is affected by cross beams. To investigate the behavior of horizontally curved I-girder bridges, it is necessary to consider curved girders with cross beams. In order to perform free vibration analyses of horizontally curved I-girder bridges, a finite element formulation is presented here and a finite element analysis program is developed. The formulation that is presented here consists of curved and straight beam elements, including the warping degree of freedom. Based on the theory of thin-walled curved beams, the shape functions of the curved beam elements are derived from homogeneous solutions of the static equilibrium equations. Third-order hermits polynomials are used to form the shape functions of the straight beam elements. In the finite element analysis program, global stiffness and mass matrix are composed, based on the Cartesian coordinate system. The Gupta method is used to efficiently solve the eigenvalue problem. Comparing the results of several examples here with those of previous studies, the formulation presented is verified. The validity of the program developed is shown by comparing results with those analyzed by the shell element.

• Journal of the Korea Convergence Society
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• v.12 no.12
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• pp.467-477
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• 2021

Purposes of this study were to identify the relationship among involvement in COVID-19 (IiC), attitude toward advertisement (AtA), and behavioral Intention to social distancing (BItSD), and to investigate the mediating effect of AtA in a loss versus gain advertising context. The participants were 379 adults who have been consecutively living in Korea since January 2020. The participants were randomly assigned to one of two advertisements using an online survey system. We found that IiC affects BItSD since Korean adults recognize the risks and seriousness of COVID-19 infection or transmission through IiC. Induced positive attitudes or emotions through involvement in the COVID-19 preventive behaviors including social distancing and vaccination are helpful to increase the BItSD. Thus, health care and information providers should give an accurate message, which induces a positive attitude, to recognize that our world is a risk of the COVID-19.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.6
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• pp.800-807
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• 2019

Objective: The aim was to evaluate backgrounding beef steers on oat + ryegrass pastures mixed with vetch and/or using energy supplementation. Methods: A randomized block design with three treatments and three replications was used. The treatments were: grass + supplement (oat + ryegrass + supplementation), legume + supplement (oat + ryegrass + vetch + supplementation) and grass + legume (oat + ryegrass + vetch). A continuous grazing system with a variable stocking rate was used. Twenty-seven intact crossbred steers (1/4 Marchigiana, 1/4 Aberdeen Angus and 2/4 Nellore) aged 7 months old and average weight of 190 kg were used. Steers were supplemented at 1% of the body weight of ground corn. The experiment lasted 84 days, between May and August 2014. Behavioral assessments were performed two times per experimental period, for 24 hours. Results: The forage mass was different between treatments, being greater for steers fed without legume. The accumulation rate, forage allowance, and stocking rate did not differ between treatments due to the adequate adjustment of forage allowance. The final weight of animals, as well as the dry matter intake (kg/d), did not differ between treatments. However, forage intake was higher for non-supplemented animals in relation to supplemented steers. Supplement intake did not alter the total digestible nutrient intake due to pasture quality. Animals fed grass + supplement had higher live weight gain per area than those fed grass + legume. Animals without supplementation spent more time in grazing. Conclusion: Feeding behavior was not altered by mixing with vetch or supplementation. Non-supplemented animals started the grazing peak earlier and spent more time in grazing than those supplemented; however, the average daily gain was similar between treatments. The live weight gain per hectare was 47% higher in pastures in which the animals received supplementation compared with those mixed with vetch, a consequence of the substitutive effect.

• The Journal of the Acoustical Society of Korea
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• v.43 no.1
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• pp.1-8
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• 2024

For measuring the performance of passive sonars, we usually consider the maximum Detection Range (DR) under the environment and system parameters in operation. In shallow water, where sound waves inevitably interacts with sea surface or bottom, detection generally maintains up to the maximum range. In deep water, however, sound waves may not interact with sea surface or/and bottom, and thus there may exist shadow zones where sound waves can hardly reach. In this situation, DR alone may not completely define the performance of each sonar. For complete description of sonar performance, we employ the concept 'Robustness Of Detection (ROD)'. In the coastal region of the East Sea, the spatial variations of water masses have close relations with DR and ROD, where the two parameters show reverse spatial variations in general. The spatial and temporal analysis of the temperature by employing the Empirical Orthogonal Function (EOF) shows that the 1-st mode represents typical pattern of seasonal variation and the 2-nd mode represents strength variations of mixed layers and currents. The two modes are estimated to explain about 92 % of the variations. Assuming two types of targets located at the depths of 5 m (shallow) and 100 m (deep), the passive sonar performance (DR) gives high negative correlations (about -0.9) with the first two modes. Most of temporal variations of temperature occur from the surface up to 200 m in the water column so that when we assume a target at 100 m, we can expect detection performance of little seasonal variations with passive sonars below 100 m.

• Korean Circulation Journal
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• v.52 no.6
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• pp.429-440
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• 2022

Background and Objectives: Endovascular therapy (EVT) first strategy has been widely adopted for the treatment of chronic limb threatening ischemia (CLTI) patients in real-world practice. This study aimed to investigate long-term outcomes of CLTI patients who underwent EVT and identify prognostic factors. Methods: From the retrospective cohorts of a Korean multicenter endovascular therapy registry, 1,036 patients with CLTI (792 men, 68.8 ± 9.5 years) were included. The primary endpoint was amputation-free survival (AFS) defined as the absence of major amputation or death. Secondary endpoints were major adverse limb events (MALE; a composite of major amputation, minor amputation, and reintervention). Results: Five-year AFS and freedom from MALE were 69.8% and 61%, respectively. After multivariate analysis, age (hazard ratio [HR], 1.476; p<0.001), end-stage renal disease (ESRD; HR, 2.340; p<0.001), Rutherford category (RC) 6 (HR, 1.456; p=0.036), and suboptimal EVT (HR, 1.798; p=0.005) were identified as predictors of major amputation or death, whereas smoking (HR, 0.594; p=0.007) was protective. Low body mass index (HR, 1.505; p=0.046), ESRD (HR, 1.648; p=0.001), femoropopliteal lesion (HR, 1.877; p=0.004), RC-6 (HR, 1.471; p=0.008), and suboptimal EVT (HR, 1.847; p=0.001) were predictors of MALE. The highest hazard rates were observed during the first 6 months for both major amputation or death and MALE. After that, the hazard rate decreased and rose again after 3-4 years. Conclusions: In CLTI patients, long-term outcomes of EVT were acceptable. ESRD, RC-6, and suboptimal EVT were common predictors for poor clinical outcomes.

• Korean Journal of Agricultural and Forest Meteorology
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• v.17 no.3
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• pp.217-226
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• 2015

To investigate the distributions of BVOCs (Biogenic Volatile Organic Compounds) from mountain near mega city and their role in forest atmospheric, BVOCs and their oxidized species were measured at a 41 m tower in Mt. Taehwa during May, June and August 2013. A proton transfer reaction-mass spectrometer (PTR-MS) was used to quantify isoprene and monoterpenes. In conjunction with BVOCs, $O_3$, meteorological parameters, PAR (Photosynthetically Active Radiation) and LAI (Leaf Area Index) were measured. The average concentrations of isoprene and monoterpenes were 0.71 ppbv and 0.17 ppbv, respectively. BVOCs showed higher concentrations in the early summer (June) compared to the late summer (August). Isoprene started increasing at 2 PM and reached the maximum concentration around 5 PM. In contrast, monoterpenes concentrations began to increase 4 PM and stayed high at night. The $O_3$ maximum was generally found at 3 PM and remained high until 5 PM or later, which was concurrent with the enhancement of $O_3$. The concentrations of BVOCs were higher below canopy (18 m) than above canopy, which indicated these species were produced by trees. At night, monoterpenes concentrations were negatively correlated with these of $O_3$ below canopy. Using MEGAN (Model of Emissions of Gases and Aerosols from Nature), the emissions of isoprene and monoterpenes were estimated at 1.1 ton/year and 0.9 ton/year, respectively at Mt. Taehwa.

• Korean Journal of Ichthyology
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• v.24 no.3
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• pp.183-190
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• 2012

The spawning site characters in the natural environment of bull-head torrent catfish, Liobagrus obesus, were investigated at the part of the Gosan stream in Korea from April to October 2010 and June 2011. The sex ratio of female to male was 1 : 1.02. Spawning season was from June to July with water temperature in approximately $23^{\circ}C$. The spawning sites were covered by the boulder used upper plate and were composed of gravel and sand. One male lies with the egg mass and guards the developing embryos in the hollow below the boulder. The environmental conditions of the spawning sites were $61.4{\pm}11.97$ (50~85) cm in depth, 0.58${\pm}0.067$ (0.48~0.72) m/sec in surface water velocity, $0.46{\pm}0.098$ (0.27~0.61) m/sec in middle water velocity, $0.27{\pm}0.083$ (0.14~0.41) m/sec in bottom water velocity. The boulder width as spawning sites was $26.2{\pm}5.32$ (20~38) cm in long axis, $20.5{\pm}2.97$ (16~25) cm in short axis and $11.1{\pm}4.02$ (5~19) cm in height. The hollow underneath the boulder was $9.8{\pm}2.32$ (6~14) cm in diameter and $2.8{\pm}1.10$ (1.5~5) cm in depth. The average number of eggs in ovary was $124{\pm}27.7$ (92~180). The matured egg size was $3.40{\pm}0.078$ (3.21~3.56) mm. The average number of spawning eggs in the spawning site was $99{\pm}12.9$ (81~122).