• 접착 및 계면
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• 제11권3호
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• pp.100-105
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• 2010

본 연구에서는 수분산 폴리우레탄에 영향을 미치는 여러 가지 요인을 알아보기 위해 monomeric diol의 종류, polyol/monomeric diol 몰비 및 dimethylolpropionic acid (DMPA)의 함량에 따라 수분산 폴리우레탄의 물성변화를 알아보았다. Polyester type polyol, 4,4-dicyclohexylmethane diisocyanate ($H_{12}MDI$), DMPA 그리고 monomeric diol을 반응시켜 prepolymer를 제조한 후 이를 연속상에 분산시켜 수분산 폴리우레탄을 합성하였다. 열적 특성에서 monomeric diol의 분자량, monomeric diol 함량 및 DMPA 함량이 증가함에 따라 $T_g$가 미세하게 증가하였다. 기계적 물성을 평가한 결과, monomeric diol의 분자량과 polyol/monomeric diol에서 monomeric diol의 함량 및 DMPA 함량이 증가함에 따라 인장강도는 증가하였다. 접착강도는 polyol/monomeric diol의 몰비가 8 : 2 일 때 가장 높은 값을 나타내었다.

• 한국미생물·생명공학회지
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• 제27권2호
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• pp.124-128
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• 1999

TK6 was able to produce NAD+ dependent cyclohexanol dehydrogenase(CDH). The production of CDH was increased rapidly at the logarithmic phase and maintained constantly after that. In order to investigate the inductive production of CDH by various substrates, the bacteria were grown in the media containing alicyclic hydrocarbons and various alcohols as a sole crabon souce. CDH was induced most actively by cyclohexanol. Cyclohexanone and cyclohexane-1,2-diol also induced remarkable amount of CDH but it was induced weakly by 1-propanol, 1-butanol, 1-pentanol, 1-hexanol, 2-propanol, and 2-methyl-1-propanol. The dehydrogenase of the bacteria grown in the media containing cyclohexanol were weakly active for various alcohols, but the dehydrogenase activity for cyclohexane-1,2-diol was twice as much as that for cyclohexanol. Activity staining on PAGE of the cell free extract of Rhodococcus sp. TK6 grown in the media containing cyclohexanol reveals at least sever isozyme bands of CDH and we nominated the four major activity bands as CDH I, II, III, and IV. CDH I was strongly induced by cyclohexanol, cyclohexane-1,2-diok, but its activity was specific to cyclohexane-1,2-diol and 1-pentanol. CDH IV was strongly induced by cyclohexanol and cyclohexane-1,2-diol, and its activity was very specific to cyclohexane-1,2-diol.

• 한국응용과학기술학회지
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• 제39권4호
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• pp.488-498
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• 2022

본 연구에서는 계면활성제 용액에서 1,3-Butanediol, 1,2-Pentanediol, 1,2 Hexanediol 들이 cmc, 표면장력, 기포력 및 유화력에 미치는 영향을 연구 하였다. 계면활성제 수용액에 diol의 첨가는 cmc와 표면장력 저하를 초래 하였으며 기포력 및 유화력은 향상 되었다. 이러한 경향은 diol의 사슬길이가 길수록 현저 하였다. 이러한 특성은 diol의 알킬사슬과 계면활성제 분자간에 소수성 상호작용이 물분자간의 응집력을 감소시키고, 계면에서 계면활성제 headgroup간 상호작용을 증진시키기 때문이다. 또한 각 diol의 방부력을 평가하기 위하여 MIC 측정을 수행 하였으며 방부력은 1,2-HDO > 1,2-PDO > 1,3-BDO 순서였다. 본 연구의 결과들은 diol들이 화장품에서 보조 계면활성제와 방부제로 이용될 수 있음을 확인 시켜 주웠다.

• Journal of Ginseng Research
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• 제9권1호
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• pp.86-94
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• 1985

Attempts were made if diol and triol fractions of ginseng saponin affect on glutamine transport into rat renal cortical mitochondria, swelling, phosphate dependent glutaminase activity, and consumption of oxygen. The following results were obtained. When mitochondrial preparation from rat renal cortex was incubated in medium containing 14C-glutamine and either triol or diol fractions, radioactivity was shown to increase at both 10-6% and 10-5% triol fractions of ginseng saponin, but reduce in case of diol fraction. The remarkable acceleration of the rate of swelling of renal cortical mitochondria was observed in the presence of 10-1% trios and diol fractions but no accerelation at lower concentrations. The activity of phosphate dependent glutaminase from renal cortical mitochondria was slightly activated at 10-2% of triol fraction. However, there was no effect in case of diol fraction. Oxygen consumption by mitochondria from renal cortex was remarkably increased at concentrations of 10-5% and 10-6% triol fractions, but reduced in the case of diol fractions. On the basis of these observations it was concluded that triol fraction of ginseng saponin might increase the transport of glutamine into mitochondria by accelerating the respiratory chain and supplying additional energy to mitochondria, and physiological role of triol fraction was entirely different from that of diol fraction of ginseng saponin.

• Journal of Applied Biological Chemistry
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• 제43권1호
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• pp.44-48
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• 2000

Burkholderia cepacia strain G4 (pHG-2) containing toluene 2-monooxygenase and toluene dioxygenase, was able to grow on toluene and accumulate cis-3-methyl-3,5-cyclohexadien-1,2-diol (cis-toluene dihydrodiol) in the liquid culture. The cis-toluene dihydrodiol produced was identical to the authentic compound, as judged through mass spectrometry and nuclear magnetic resonance analysis. Our results indicate that pHG-2 provides an economical means to produce chemically-important chiral synthons while growing on toluene.

• 한국식품과학회지
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• 제22권5호
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• pp.549-554
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• 1990

비이온성 수지인 Amberlite XAD-2 흡착 및 메탄올 용출법에 의해 살구로부터 배당체분획을 얻은 다음 almond ${\beta}-glucosidase$ 및 산가수분해(pH3.0) 또는 pH 3.0에서 simultaneous distillation-extraction(SDE)에 의해 얻어진 가수분해물로부터 GC 및 GC-MS에 의해 40종의 휘발성 성분을 확인하였다. 3종의 가수분해물로부터 이미 배당체로서 살구에 존재하는 것으로 알려져 있는 linalool oxide, linalool, ${\alpha}-terpineol$, nerol, geraniol, benzyl alcohol 및 2-phenylethyl 이외에도 2,6-dimethyl-3,7-octadiene-2,6-diol, 3,7-dimethyl-1,5-octadiene-3,7-diol,(E)-및 (Z)-2,6-dimethyl-2,7-octadiene-1,6-diol, $3-4-dihydro-{\beta}-ionol$ $3-oxo-{\alpha}-ionol$, $3-hydroxy-7,8-dihydro{\beta}-ionol$, $3-oxo-7,8-dihydro-{\alpha}-ionol$, $3-hydroxy-{\beta}-ionone$, eugenol, 3-hydroxyethylphenyl acetate 및 2,3-dihydrobenzofuran이 살구에서 처음으로 확인되었다.

• Journal of Microbiology and Biotechnology
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• 제12권1호
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• pp.39-45
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• 2002

Activity staining on the native polyacrylamide gel electrophoresis (PAGE) of a cell-free extract of Rhodococcus sp. TK6, grown in media containing alcohols as the carbon source, revealed at least seven isozyme bands, which were identified as alcohol dehydrogenases that oxidize cyclohexanol to cyclohexanone. Among the alcohol dehydrogenases, cyclohexanol dehydrogenase II (CDH II), which is the major enzyme involved in the oxidation of cyclohexanol, was purified to homogeneity. The molecular mass of the CDH II was determined to be 60 kDa by gel filtration, while the molecular mass of each subunit was estimated to be 28 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The CDH II was unstable in acidic and basic pHs, and rapidly inactivated at temperatures above $40^{\circ}C$ . The CDH II activity was enhanced by the addition of divalent metal ions, like $Ba^2+\;and\;Mg^{2+}$. The purified enzyme catalyzed the oxidation of a broad range of alcohols, including cyclohexanol, trans-cyclohexane-1,2-diol, trans-cyclopentane-l,2-diol, cyclopentanol, and hexane-1,2-diol. The $K_m$ values of the CDH II for cyclohexanol, trans-cyclohexane-l,2-diol, cyclopentanol, trans-cyclopentane-l,2-diol, and hexane-l,2-diol were 1.7, 2.8, 14.2, 13.7, and 13.5 mM, respectively. The CDH II would appear to be a major alcohol dehydrogenase for the oxidation of cyclohexanol. The N-terminal sequence of the CDH II was determined to be TVAHVTGAARGIGRA. Furthermore, based on a comparison of the determined sequence with other short chain alcohol dehydrogenases, the purified CDH II was suggested to be a new enzyme.

• 한국해양학회지:바다
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• 제22권2호
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• pp.45-55
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• 2017

Long chain alkyl diols (LCDs)은 다양한 해양 환경 퇴적물에서 관측되고 있다. Rampen et al. (2012)은 해양 표층 퇴적물에서 분석된 LCDs 중 $C_{30}$ 1,15-diol, $C_{28}$ 1,13-diol, $C_{30}$ 1,13-diol를 이용하여 Long chain Diol Index (LDI)라는 고수온 프록시를 제시하였다. 일반적으로 LCDs의 정성 및 정량 분석은 CP-Sil5CB와 DB-5ms 컬럼을 사용해 GC-MS를 주 기반으로 한다. 본 연구에서는 서로 다른 해양환경(동해 및 서북극해)에서 획득한 해양퇴적물을 활용하여 특성이 다른 세가지 GC 컬럼(CP-Sil5CB, HP-5ms, DB-5)이 LCDs의 정량 분석에 미치는 영향을 검토하였다. 본 연구를 통해 일반적으로 CP-Sil5CB로 분석된 농도 결과가 HP-5ms와 DB-5로 분석된 농도 결과와 통계적으로 유의한 차이가 있는 것으로 확인되었다. 하지만 LDI로 복원된 표층수온의 컬럼 간 편차는 동해 퇴적물의 경우 $0.1-0.2^{\circ}C$, 서북극해 퇴적물의 경우 $0.2-0.7^{\circ}C$로 LDI의 calibration error 범위(${\pm}1{\sigma}$) 보다 작았다. 결론적으로 본 연구는 컬럼에 따라 LCDs의 정량 결과는 현저한 차이를 보일 수 있지만, LDI 프록시 값에 미치는 영향은 상대적으로 미비함을 보여 주었다. 따라서 LDI 프록시를 활용한 동해 및 서북극 해양 퇴적물의 고수온 복원에 특성이 다른 컬럼을 사용 할 수 있음을 시사하였다.

• 한국자기공명학회논문지
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• 제10권1호
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• pp.89-95
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• 2006

The methanolic extract of the marine sponge Raspilia hirsute collected from Keomun Island resulted in three types of sterols: a mixture of (24S)-Poriferasta-5, 25-diene-$3\beta$, 24-diol and (24R)-Stigmasta-5, 25-diene-$3\beta$, 24 -diol (1), 25,26,27-Trinorcholest-5-en-$3\beta$,24-diol (2), and Pregn-5-en-20-on-$3\beta$-ol (3). The isolation and structural determination of these sterols are reported here. Compound 1 showed moderate cytotoxicity against human Leukemia cell line K562.

• 한국동물학회지
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• 제31권2호
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• pp.142-146
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• 1988

PAH화합물의 하나인 benzo(a)pyrene의 쥐 태반 microsomes에 의한 대사활성화를 조사하였다. B(a)P대사물은 C18- $\mu$ Bondapak칼럼을 사용하여 고압액체크로마토그래피로 분석하였다. 그 결과 B(a)P를 투여한 쥐의 태반 microsomes에 의한 주 대사산물은 발암성이 강한 7,8-diol B(a)P였으며, 또한 적은량의 4,5-diol B(a)P와 quinone류가 검출되었다. 3-methyl-cholanthrene을 투여한 경우 hydroxy B(a)P 와 quinone화합물이 주 대사산물이었으며 Phenobarbital을 전처리했을 경우 7,8-diol B(a)P이 주 대사산물인 것으로 나타났다.