Journal of Applied Biological Chemistry
- Volume 43 Issue 1
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- Pages.44-48
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- 2000
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- 1976-0442(pISSN)
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- 2234-7941(eISSN)
Burkholderia cepacia Strain G4 (pHG-2) Accumulates cis-3-Methyl-3,5-cyclohexadien-1,2-diol While Growing on Toluene
- Hur, Hor-Gil (Department of Soil, Water and Climate, University of Minnesota)
- Received : 2000.02.18
- Published : 2000.03.31
Abstract
Burkholderia cepacia strain G4 (pHG-2) containing toluene 2-monooxygenase and toluene dioxygenase, was able to grow on toluene and accumulate cis-3-methyl-3,5-cyclohexadien-1,2-diol (cis-toluene dihydrodiol) in the liquid culture. The cis-toluene dihydrodiol produced was identical to the authentic compound, as judged through mass spectrometry and nuclear magnetic resonance analysis. Our results indicate that pHG-2 provides an economical means to produce chemically-important chiral synthons while growing on toluene.
Keywords
- Burkholderia cepacia G4;
- cis-toluene dihydrodiol;
- synthon;
- toluene dioxygenase;
- cis-3-methyl-3,5-cyclohexadien-1,2-diol