• Toxicological Research
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• v.14 no.2
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• pp.123-127
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• 1998

BRCA1 is a tumor suppresser gene in familial cases of breast cancer. It has been controversial whether the subcellular localization of BRCA1 is located in nuclei or cytoplasm in normal human breast cells. We found that a p220 protein was expressed in Type II Normal human breast epithelial cells (NHBEC) but not in Type I NHBEC in Western blot analysis using the 17F8 (3A2) antibody. Immunostaining using the same antibody revealed positive staining in nuclei, cytoplasm and perinuclei of Type II cells and negative staining in Type I NHBEC. The p220 protein, however, was expressed in SV40 immortalized Type I NHBEC and tumorigenic cells derived from them after x-ray and neu oncogene treatment. The subcelluar localization was mostly cytoplasmic and punctate in the nuclei. The breast carcinoma cell lines, MCF-7 and T47D, also expressed the p220 protein. Using RT-PCR, we observed the expression of BRCA1 mRNA in both Type I and Type II NHBEC. This result indicated that there might be mechanisms involved in post-translational or translational regulation of BRCA1 gene. It is speculated that the absence of BRCA1 protein expression in Type I NHBEC might playa role in their susceptibility to neoplastic transformation.

• Clinical and Experimental Reproductive Medicine
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• v.19 no.2
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• pp.175-179
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• 1992

The factors involved in the initial neoplastic transformation and subsquent growth of uterine fibroid are poorly understood. The reduction in uterine fibroid volume associated with the chronic administration of the mechanisms mediating the decrease in fibroid volume in GnRH-a treated patients are poorly defined. The purpose of this study was to determine the proliferating cell nuclear antigen(PCNA) in fibroid from-women pretreated with GnRH analogue(GnRH-a) compared with controls. Tissue was obtained from 16 premenopausal women with uterine fibroid who received GnRH-a(D-Trp6-GnRH) intramusculary every 28 days for four injections. The mean proliferating index(PI) in patients with uterine fibroids was $2.25{\pm}0.9$, and in controls was $8.82{\pm}1.8$(P<0.001). The proliferating index was not corrleated with the reduction of fibroid volume. In this clinical study, although hypoestrogenism may be the main factor that reduce the volume of fibroid, other factors are also considered to be involved in that process. And the regrowth of uterine fibroid may be affected by increased production of PCNA after stopping GnRH-a.

• Journal of the Korean Institute of Intelligent Systems
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• v.7 no.2
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• pp.34-42
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• 1997

This paper deals with the recognition of closed planar shape using a three dimensional spectral feature vector which is derived from the FFT(Fast Fourier Transform) spectrum of contour sequence and fuzzy ARTMAP neural network classifier. Contour sequences obtained from 2-D planar images represent the Euclidean distance between the centroid and all boundary pixels of the shape, and are related to the overall shape of the images. The Fourier transform of contour sequence and spectrum analyzer are used as a means of feature selection and data reduction. The three dimensional spectral feature vectors are extracted by spectrum analyzer from the FFT spectrum. These spectral feature vectors are invariant to shape translation, rotation and scale transformation. The fuzzy ARTMAP neural network which is combined with two fuzzy ART modules is trained and tested with these feature vectors. The experiments including 4 aircrafts and 4 industrial parts recognition process are presented to illustrate the high performance of this proposed method in the recognition problems of noisy shapes.

• Journal of Environmental Health Sciences
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• v.36 no.4
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• pp.323-336
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• 2010

We evaluated the properties of a fixed-bed column reactor for high-concentration tetrachloroethylene (PCE) removal. The anaerobic bacterium Clostridium bifermentans DPH-1 was able to dechlorinate PCE to cis-1,2-dichloroethylene (cDCE) via trichloroethylene (TCE) at high rates in the monoculture biofilm of an upflow fixed-bed column reactor. The first-order reaction rate of C. bifermentans DPH-1 was relatively high at $0.006\;mg\;protein^{-1}{\cdot}l{\cdot}h^{-1}$, and comparable to rates obtained by others. When we gradually raised the influent PCE concentration from $30\;{\mu}M$ to $905\;{\mu}M$, the degree of PCE dechlorination rose to over 99% during the operation period of 2,000 h. In order to maintain efficiency of transformation of PCE in this reactor system, more than 6 h hydraulic retention time (HRT) is required. The maximum volumetric dechlorination rate of PCE was determined to be $1,100\;{\mu}mol{\cdot}d^{-1}l$ of reactor $volume^{-1}$, which is relatively high compared to rates reported previously. The results of this study indicate that the PCE removal performance of this fixed-bed reactor immobilized mono-culture is comparable to that of a fixed-bed reactor mixture culture system. Furthermore, our system has the major advantage of a rapid (5 days) start-up time for the reactor. The flow characteristics of this reactor are intermediate between those of the plug-flow and complete-mix systems. Biotransformation of PCE into innocuous compounds is desirable; however, unfortunately cDCE, which is itself toxic, was the main product of PCE dechlorination in this reactor system. In order to establish a system for complete detoxification of PCE, co-immobilization of C. bifermentans DPH-1 with other bacteria that degrade cDCE aerobically or anaerobically to ethene or ethane may be effective.

• Transactions on Electrical and Electronic Materials
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• v.16 no.1
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• pp.10-15
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• 2015

A thin-film transmission line (TFTL) employing a microstrip line/coplanar waveguide (ML/CPW) was fabricated on a silicon substrate for application to a miniaturized on-chip RF component, and the RF characteristics of the device with the proposed structure were investigated. The TFTL employing a ML/CPW composite structure exhibited a shorter wavelength than that of a conventional coplanar waveguide and that of a thin-film microstrip line. When the TFTL with the proposed structure was fabricated to have a length of ${\lambda}/8$, it showed a loss of less than 1.12 dB at up to 30 GHz. The improvement in the periodic capacitance of the TFTL caused for the propagation constant, ${\beta}$, and the effective permittivity, ${\varepsilon}_{eff}$, to have values higher than those of a device with only a conventional coplanar waveguide and a thin film microstrip line. The TFTL with the proposed structure showed a ${\beta}$ of 0.53~2.96 rad/mm and an ${\varepsilon}_{eff}$ of 22.3~25.3 when operating from 5 to 30 GHz. A highly miniaturized impedance transformer was fabricated on a silicon substrate using the proposed TFTL for application to a low-impedance transformation for broadband. The size of the impedance transformer was 0.01 mm2, which is only 1.04% of the size of a transformer fabricated using a conventional coplanar waveguide on a silicon substrate. The impedance transformer showed excellent RF performance for broadband.

• Journal of Korea Water Resources Association
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• v.35 no.5
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• pp.485-500
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• 2002

Hydraulic model was developed to analyze the complex flow due to channel structures, tide, and tributaries in the lower Han river and Imjin river. DWOPER-2K model which can automatically process the data transformation in the model was developed as the 1-D hydraulic routing model. Observed data in tidal zone and the recent channel geometry data were collected for hydraulic model. And the flow over the Jamsil and Singok submerged weir was analyzed properly and roughness coefficient was optimized to each regions and each discharges. By the results of verification of the model, the model developed in this study may contribute to improvement of the accuracy of flood forecasting and channel management because this model can efficiently and properly analyze the various kind of flow occurred in the region of the lower Han river and Imjin river.

• Journal of Biosystems Engineering
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• v.28 no.1
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• pp.65-76
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• 2003

The analysis of cow body parameters is important to provide some useful information fur cow management and cow evaluation. Present methods give many stresses to cows because they are invasive and constrain cow postures during measurement of body parameters. This study was conducted to develop the stereo vision system fur non-invasive analysis of cow body features. Body feature parameters of 16 heads at two farms(A, B) were measured using scales and nineteen stereo images of them with walking postures were captured under outdoor illumination. In this study, the camera calibration and inverse perspective transformation technique was established fer the stereo vision system. Two calibration results were presented for farm A and fm B, respectively because setup distances from camera to cow were 510 cm at farm A and 630cm at farm B. Calibration error values fer the stereo vision system were within 2 cm for farm A and less than 4.9 cm for farm B. Eleven feature points of cow body were extracted on stereo images interactively and five assistant points were determined by computer program. 3D world coordinates for these 15 points were calculated by computer program and also used for calculation of cow body parameters such as withers height. pelvic arch height. body length. slope body length. chest depth and chest width. Measured errors for body parameters were less than 10% for most cows. For a few cow. measured errors for slope body length and chest width were more than 10% due to searching errors fer their feature points at inside-body positions. Equation for chest girth estimated by chest depth and chest width was presented. Maximum of estimated error fur chest girth was within 10% of real values and mean value of estimated error was 8.2cm. The analysis of cow body parameters using stereo vision system were successful although body shape on the binocular stereo image was distorted due to cow movements.

• Journal of the Institute of Convergence Signal Processing
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• v.8 no.4
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• pp.285-292
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• 2007

SoC which deploys software modules as well as hardware IPs on a single chip is a major revolution taking place in the implementation of a system design, and high-level synthesis is an important process of SoC design methodology. Recently, SPARK parallelizing high-level synthesis software tool has been developed. It takes a behavioral ANSI-C code as an input, schedules it using code motion and various code transformations, and then finally generates synthesizable RTL VHDL code. Although SPARK employs various loop transformation algorithms, the synthesis results generated by SPARK are not acceptable for basic signal and image processing algorithms with nested loop. In this paper we propose a SoC platform with an application-specific PLD targeting local operations which are feature of many loop algorithms used in signal and image processing, and demonstrate design process which maps behavioral specification with nested loops written in a high-level language (ANSI-C) onto 2D systolic array. Finally the derived systolic array is implemented on the proposed application-specific PLD of SoC platform.

• Korean Journal of Applied Biomechanics
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• v.16 no.1
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• pp.139-149
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• 2006

This study is to analyze the kinematic variables of inward $1{\frac{1}{2}}$ somersault in platform diver. For the manner, 3 people form the national diving team in the year 2000were chosen as the subjects and two S-VHS video cameras set in 60frames/sec were used for recording their motions. Coordinated raw positions data through digitizing are smoothing by butter-worth's low-pass filterin method at a cut off frequency 6.0Hz. and the direct linear transformation(DLT) method was employed to obtain 3-D position coordinates. The conclusions were as follows. However, horizontal distance which is the change of the COG, form the point of the jump to the point of Event 3 where the player is out of the board range completely, Subject B showed 105.1cm and 71.1cm of the vertical distance which are shorter horizontal distance and higher vertical distance, thus, took a great advantage of the position to prepare for the entry. Therefore, if a player takes higher position by speeding up the vertical velocity at the moment of the jumping off the board, and stays in the air longer, the player can have more time to show his skill. Because of the use of the characteristics of the inward somersault, keeping the safe distance form the board is important but in order to higher the completeness, it is ideal to keep the horizontal distance little over 100cm. Also, the angles of shoulder and elbow from Event 1 to 4, depending on swing of the arms, motions in the air, getting ready for the entry, showed some difference individual by individual, according to the velocity of the thigh and shank showed much difference while getting ready and take-off, and it's because of the individual's different bending and straightening for horizontal and vertical distance.

• Korean Journal of Applied Biomechanics
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• v.14 no.1
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• pp.13-26
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• 2004

The purpose of this study was to investigate the differences of the kinematical and the kinetical factors that calculated from preflight to preflight of salto forward straight 3/2 turn motion between skillers and less-skillers. four S-VHS video cameras operating at 60Hz were used to record the performances. Five elite male gymnasts were participated in this study as subjects. Three-dimensional coordinates of 21 body landmarks during each trial were collected using a Direct Linear Transformation method. The raw 3-D coordinates of the 21 body landmarks were smoothed using a second order lowpass, recursive Butterworth digital filter and a cutoff frequency of 10Hz. Load cells attached on the beneath of a board were used to attain the kinetic variables. It was found that the more angular momentum in the longitudinal axis, the less vertical velocity and these angular momentum effected the height of peak in the preflight. Also, it was revealed that the larger angular momentum in the medio-lateral axis was rather than it in the longitudinal axis to increase vertical height and rotation force of the body. For the reaction force of springboard, the vertical and the horizontal reaction force were 16.52BW and 3.45BW, respectively. It was found that the higher value of the vertical reaction force induced the faster vertical velocity and the higher an ar momentum. of the whole body center of gravity.