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Growth Inhibition of Toxic Cyanobacterium Microcystis aeruginosa by Various SNPs (Silver Nanoparticles) (여러 가지 은나노 물질의 유해 남조 Microcystis aeruginosa 생장억제)

  • Park, Myung-Hwan;Kim, Keun-Hee;Lee, Huk-Hee;Kim, Jin-Seog;Hwang, Soon-Jin
    • Korean Journal of Ecology and Environment
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    • v.42 no.1
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    • pp.75-84
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    • 2009
  • The effect of various SNPs (silver nanoparticles) on the growth of Microcystis aeruginosa was investigated in laboratory and field experiment. Four SNPs, namely JS47N, JS47N-K2, JS47N/3-1 and JS47N/3-2 were used to this study. The Ag size, concentration and color of these solutions were about $20{\sim}40nm$, $200mg\;L^-1$ and brown, respectively. At 0.01 and $0.1mg\;L^-1$, SNPs inhibited the growth of unicellular M. aeruginosa by 99.4% and 99.9%, respectively. However, SNPs of $1mg\;L^-1$ inhibited the growth of colonial M. aeruginosa by 98.5%, whereas the other three concentrations (0.001, 0.01 and $0.1mg\;L^-1$) had little inhibitory effect. In experimental enclosures from eutrophic lake, cyanobacteria including M. aeruginosa were found to be more sensitive to the SNPs than green algae and diatoms. In conclusion, our study indicates that SNPs has a selective cyanocidal potential when used to M. aeruginosa. We believe that future studies need to test on various other organisms, and determine minimum concentration for field application.

The Environment-Friendly High Quality Pork Production by feeding the Fermentation Products of Natural Clay Mineral (천연의 점토광물질 발효산물 급여에 의한 친환경 고품질 원료돈육 생산)

  • Cho, Jin Kook;Yang, Seung Hak;Hwang, Seong Gu
    • Journal of Animal Environmental Science
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    • v.18 no.sup
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    • pp.55-64
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    • 2012
  • We investigated the effect of the clay mineral fortified with complex trace elements by specific fermentation microbes on growth performance and a quality of pork in this study. For the declared experimental animal, 80 heads of crossbred with Large White and Yorkshire were randomly splitted into a control group and a test group. Each group were assigned with 4 replicates and 10 heads of each replicate. When 0.3% of the fermentation product of the clay mineral (FCM) was added into feed stuff and fed for 9 weeks of experimental feeding period, the results of the feed intake, weight gains, pH of carcass, lightness of meat color, and brightness of the muscle semimembranosus were similar to those of the control group. However, dressing percentage, reddishness of carcass, and water holding capacity were improved, respectively, as much as 2.7%, 12%, and 10%, and reduced by 6% in cooking loss. In order to investigate the immune modulatory effect of fermented clay mineral, pheripheral blood mononuclear cells (PBMC) were isolated and Immune modulatory parameters were measured. The proliferation activity of PBMC from pigs fed the fermented clay mineral were significantly increased compared to control group pigs, and also those results were more clearly observed as activated with lipopolysaccharide and concanavaline A. The secretion of TNF-${\alpha}$ of the FCM group pigs showed an increasing tendency. Therefore it was suggested that the feeding of FCM which was high in cation metathesis and the value of infrared ray, activated the immune responses, and thus the production of the environment-friendly high quality pork without the use of antibiotics would be possible.

Feeding Effect of Citrus Byproduct on the Quality of Cross-bred Black Pig in Jeju Island (육성기 및 비육기에 급여한 감귤 부산물이 제주도 개량 흑돼지 고기의 품질특성에 미치는 영향)

  • Yang, Seung-Joo;Koh, Seok-Min;Yang, Tae-Ik;Jung, In-Chul;Moon, Yoon-Hee
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.7
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    • pp.897-902
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    • 2006
  • This study was carried out to investigate the possibility for utilization of waste materials and the characteristics of pork by feeding citrus byproducts. The groups consisted of the pork without citrus byproduct (TB-0) and the pork fed with 6% and 10% citrus byproduct during growing and fattening period (TB-1). The contents of moisture, crude protein, crude fat, crude ash, vitamin $B_1$ and $B_2$, total amino acids, and caloric were not significantly different between TB-0 and TB-1 (p>0.05). The contents of cholesterol and the magnesium of TB-1 were lower than those of TB-0. Palmitic acid was the most abundant among saturated fatty acids while oleic acid was the most abundant among unsaturated fatty acids in both groups. In case of sensory score, the color and aroma of raw meat and taste, texture, juiciness and palatability of boiled meat were not influenced by feeding of citrus byproduct. But the flavor preference of boiled meat of TB-1 was higher than that of TB-0.

Characteristics of agronomy to vitamin A strengthening rice at large scale GMO field (대규모 GMO 포장에서 비타민 A 강화 벼의 농업특성 검정)

  • Lee, Hyun-Suk;Ryu, Tae-Hun;Jung, Hee-Young;Park, Soon-Ki;Park, Gyu-Hwan;Sohn, Jae-Keun;Kim, Kyung-Min
    • Korean Journal of Breeding Science
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    • v.42 no.1
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    • pp.56-60
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    • 2010
  • This study was carried out to develop of macro-protocol and the biosafety guide Golden Rice, modified vitamin A at large scale GMO field. Commercialization of genetically modified (GM) plants will be required the assessment of risks associated with the release of GM plants that should include a detailed risk assessment of their impacts in the environment and human health. Prior to GM plant release, applicants should provide the information on GM crops for approval. A total of $4,700m^2$ GM field, each of Golden Rice and 'Nakdong' were used for further analysis on agronomic traits and pathogenesis. Generally plant growth and panicle number in the transgenic lines were lower than in the wild type under field condition. The major agronomic traits such as plant growth, grain quality in Golden Rice were similar than in 'Nakdong'. The yield difference was due to reduced ripened rate and the 1,000 grain weight. The grain shape was similar donor plant, however, color of brown rice was different from segregation. Also the difference of pathogenesis reaction was not significant in the leaf blast and sheath blight between Golden Rice and 'Nakdong'. These results provided the agronomic data and pathogenesis for risk assessment analysis of Golden Rice and suggested that the macro-protocol could be useful to detect GM plants.

Changes in physical characteristics of white pan bread by addition of GABA rice bran and its extract (GABA 미강 및 미강추출물 첨가에 의한 식빵의 텍스처 및 저장성의 변화)

  • Oh, Su-Jin;Kwon, Young-Hoi;Shin, Hae-Hun;Kim, Hyun Soo;Choi, Hee-Don;Lim, Seung-Taik
    • Korean Journal of Food Science and Technology
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    • v.50 no.6
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    • pp.614-620
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    • 2018
  • A rice bran physically treated to increase the residual gamma amino butyric acid (GABA) content (200 mg/100 g) or its hot-water extract (200 mg/100 g) was added into a white pan bread, and changes in the physical properties including color, and volume and texture changes during storage at room temperature were examined. The addition of bran powders had negative effects on bread quality and storage stability whereas that of rice bran extract (RBE) improved the storage stability of bread. The lightness of bread crumbs decreased but the volume of bread slightly increased after addition of the RBE. The increase in crumb hardness during storage was retarded by the RBE addition. The residual concentration of GABA in bread was increased 38-fold when 20% of RBE was added. The addition of RBE to white pan bread improved the resistance to staling and health-promoting function because of GABA.

Absorption Spectroscopic Studies of Prodigiosin Extracted from Serratia Marcescens Strain (Serratia marcescens 균주로부터 추출한 Prodigiosin의 흡수분광학적 연구)

  • Park, Hee-Aurk
    • Journal of the Korean Applied Science and Technology
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    • v.36 no.1
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    • pp.355-361
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    • 2019
  • The red pigment extracted from Serratia marcescens 2354 (ATCC 25419) was prodigiosin (PG), which was dissolved in methanol and measured for ultraviolet and visible light absorption spectra. It was the typical absorption spectrum of PG in an acid solution with ${\lambda}_{max}=537nm$. When the concentration of PG was increased from $1.0{\times}10-5M$ to $9.0{\times}10-5M$ in the methanol solution, the absorption intensity at 537 nm was increased, the absorption intensity at 467 nm was decreased, and the isosbestic point at 500 nm was observed. This phenomenon can be regarded as a result of reversible acid-base equilibrium reaction considering 537 nm and 467 nm of PG absorption band in acid and base solution respectively and isosbestic point of 500 nm. On the other hand, when the concentration of PG was reduced from $6.0{\times}10-4$ to $1.0{\times}10-4M$ in acetic acid buffer solution at pH 4.75, a new absorption band with ${\lambda}$ max at 500 nm appeared. This absorption band appears only in the aqueous solution of pH 4.75 and does not appear in the pure methanol solution of the same pH. This is due to the conversion of the PG molecule from the ${\alpha}$-isomer to the ${\beta}$-isomer by $H_2O$. In other words, it was confirmed that the color change of the PG can be caused by the concentration of the solution and the characteristics of the solvent.

Study on Detection for Cochlodinium polykrikoides Red Tide using the GOCI image and Machine Learning Technique (GOCI 영상과 기계학습 기법을 이용한 Cochlodinium polykrikoides 적조 탐지 기법 연구)

  • Unuzaya, Enkhjargal;Bak, Su-Ho;Hwang, Do-Hyun;Jeong, Min-Ji;Kim, Na-Kyeong;Yoon, Hong-Joo
    • The Journal of the Korea institute of electronic communication sciences
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    • v.15 no.6
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    • pp.1089-1098
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    • 2020
  • In this study, we propose a method to detect red tide Cochlodinium Polykrikoide using by machine learning and geostationary marine satellite images. To learn the machine learning model, GOCI Level 2 data were used, and the red tide location data of the National Fisheries Research and Development Institute was used. The machine learning model used logistic regression model, decision tree model, and random forest model. As a result of the performance evaluation, compared to the traditional GOCI image-based red tide detection algorithm without machine learning (Son et al., 2012) (75%), it was confirmed that the accuracy was improved by about 13~22%p (88~98%). In addition, as a result of comparing and analyzing the detection performance between machine learning models, the random forest model (98%) showed the highest detection accuracy.It is believed that this machine learning-based red tide detection algorithm can be used to detect red tide early in the future and track and monitor its movement and spread.

Effects of Conjugated Linoleic Acid (CLA) Precursor and Produce Supplementation on Egg Production, Egg Quality and Blood Immunological Parameters in Laying Hens (Conjugated Linoleic Acid (CLA) 전구체 및 정제 부산물의 첨가가 산란계의 산란율, 계란 품질 및 면역 관련 혈액학적 지표에 미치는 영향)

  • Yoo, J.S.;Shin, S.O.;Cho, J.H.;Chen, Y.J.;Kim, H.J.;Huang, Y.;Kim, Y.J.;Hwang, K.Y.;Kim, J.W.;Kim, I.H.
    • Korean Journal of Poultry Science
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    • v.34 no.3
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    • pp.173-180
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    • 2007
  • This study was conducted to evaluate conjugated linoleic acid (CLA) precursor and produce supplementation on egg production, egg quality and blood immunological parameters in laying hens. The total of 252 (32-wk) Hy-line brown commercial hens were used for 5 weeks. Dietary treatments included 1) CON (basal diet + oat 1% + soybean oil 1%), 2) WOS (basal diet + whey 0.5% + oat 0.5% + soybean oil 1%), 3) WS (basal diet+ whey 1% + soybean oil 1%), 4) WOCLA (basal diet + whey 0.5% + oat 0.5% + CLA produce 1%), 5) WCLA (basal diet + whey 1% + CLA produce 1%) and 6) FOCLA (basal diet + Fish oil 0.5% + oat 1% + CLA produce 0.5%). For 4 weeks egg shell thickness was higher in WOS and FOCLA treatments than WOCLA and WCLA treatments (P<0.05). Egg weight was significantly increased (P<0.05) in WOS and FOCLA treatments compared to WS and WCLA treatments for 4 weeks. From 4 to 5 weeks yolk high, yolk color and haugh unit were significantly higher in FOCLA treatment than CON treatment (P<0.05). IgG concentration of blood was increased in WOS and FOCLA treatments compared to CON, WS and WOCLA treatments(P<0.05). In conclusion, fish oil, CLA produce and oat supplementation in laying hens diet improve egg shell thickness, yolk high, yolk color and haugh unit, also, CLA precursor supplementaion in laying hens diet improve IgG concentration of blood higher than CON treatment.

Physicochemical Composition of Buckwheat Microgreens Grown under Different Light Conditions (다른 광조건 하에서 재배된 메밀 새싹채소의 이화학적 특성)

  • Choi, Mi-Kyeong;Chang, Moon-Sik;Eom, Seok-Hyun;Min, Kwan-Sik;Kang, Myung-Hwa
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.5
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    • pp.709-715
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    • 2015
  • As consumers interest in microgreens is increasing worldwide, the production of leafy microgreens uisng different LED lights was investigated in this study. The experiment was carried out to evaluate the effects of different LED lights on the composition and vitamin C contents of buckwheat microgreens. Physicochemical properties of buckwheat microgreens grown under different lights (red, blue, and white) and control exposed to a dark room were investigated. Moisture contents of buckwheat microgreens were 95.65% under white light (WL), 95.75% under blue light (BL), 90.77% under red light (RL), and 89.71% under dark room (DR). Crude ash contents of buckwheat microgreens grown under WL, DR, RL, and BL were 0.39%, 0.39%, 0.31%, and 0.37%, respectively. Crude protein contents of buckwheat microgreens grown under DR, RL, WL, and BL were 7.12%, 7.81%, 1.60%, and 2.40%, respectively. Crude fat contents of buckwheat microgreens grown under DR, BL, RL, and WL were 1.12%, 0.54%, 0.35%, and 0.22%, respectively. $^{\circ}Brix$ was the highest in microgreens grown under BL and RL and the lowest in buds grown under DR. Vitamin C content was the highest in buds grown under WL and the lowest in buds grown under BL. Total chlorophyll content was the highest in microgreens grown under RL and the lowest in buds grown under WL. For mineral content measurement of buckwheat microgreens, Ca, K, Mg, and P contents were high whereas B, Cu, and Zn contents were not detected. The mineral contents of buckwheat microgreens according to each color of light showed significant differences. These results demonstrated that treatment of different colored LED lights during cultivation was able to increase vitamin C content up to affecting the nutritional value of buckwheat microgreens.

Studies on LED Wavelength to Enhance Growth and Bio-active Compounds of Carrots (당근의 성장과 생리활성물질 함량을 증진시키는 LED 파장에 관한 연구)

  • Kang, Suna;Kim, Min-Jung;Kim, Bong Soo;Park, Sunmin
    • Journal of Applied Biological Chemistry
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    • v.58 no.2
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    • pp.131-137
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    • 2015
  • Commercial greenhouse plant factories are highly efficient for controlling external factors such as floods, drought, insects, air pollution etc. However, they require substantial startup & maintenance investments and experimental research to optimize production. These facilities are especially useful for urban farming where high efficiency in small spaces is required. In this study, we investigated whether light emitting diode (LED) lights with mixed dominant wavelengths (650 nm : 550 nm : 445 nm=8:1:1, 650 nm : 445 nm=6:4) can increase the growth rate and bio-active compound content of carrots in comparison to that of fluorescent light (FL). LED with mixed wavelength (650 nm : 550 nm : 445 nm=8:1:1) increased the total weight and root circumference of carrots compared to FL. However, ${\beta}$-carotene contents were not significant in LED (650 nm : 550 nm : 445 nm=8:1:1). However, LED (650 nm : 445 nm=6:4) increased the ${\beta}$-carotene (FL: 7.27, LED: 10.48 mg/g ${\beta}$-carotene dried weight). These results suggested that using LED light at the ideal wavelength, at the antithesis color of the plant, might enhance plant growth and bio-active compound contents.