• Asian-Australasian Journal of Animal Sciences
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• v.17 no.8
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• pp.1118-1122
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• 2004

The study was conducted to evaluate the effect of chromium nanoparticle (CrNano) on growth, carcass characteristics, pork quality, and tissue chromium in finishing pigs. A total of 100 Duroc$\times$Landrace$\times$Yorkshire barrows (average initial BW of 64.78$\pm$0.83 kg) were divided into 10 pens, 10 pigs per pen. 5 pens of pigs were fed with control diet (corn-soybean meal, 1.05% Lys) and others were fed with control diet addition with 200 $\mu$g/kg Chrome (Cr) from CrNano. During the trial, all pigs were given free access to feed and water. After 35 days trial, 10 pigs from each each treatment (weight similar to average group weight, 91.34$\pm$1.31 kg for control group, and 92.26$\times$0.56 kg for CrNano treated group) were sacrificed to measure carcass characteristics, pork quality, and to collect the samples of longissimus muscle, liver, kidney and heart. The results indicated that the addition of 200 $\mu$g/kg CrNano decreased feed: gain ratio by 3.56% (p<0.05) compared to the control group. Pigs fed CrNano had 14.06% higher (p<0.05) carcass lean percentage, 19.96% (p<0.05) larger longissimus muscle area and 25.53% lower (p<0.05) carcass fat percentage, 18.22% lower (p<0.05) backfat thickness. Drip loss in chops from pigs fed CrNano was decreased by 21.48% (p<0.05) and weights of longissimus muscle and semimembranosus were increased by 16.33% (p<0.05) and 14.87% (p<0.05) respectively. In addition, supplemental CrNano resulted in 184.11% (p<0.05), 144.99% (p<0.05), 88.13% (p<0.05) and 52.60% (p<0.05) increment of Cr concentration in longissimus muscle, liver, kidney and heart, respectively. These results suggest that supplemental CrNano has beneficial effects on carcass characteristics, pork quality and individual skeletal muscle weight, increase tissue chromium concentration in selected muscle and organs.

• Economic and Environmental Geology
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• v.53 no.5
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• pp.529-542
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• 2020

The cesium (Cs) sorption characteristics of a bead-type polysulfone carrier contained HNO₃-treated bamboo charcoal (3 - 5 mm in diameter) in water system were investigated and its Cs removal efficiency as an adsorbent from water was also identified by various laboratory experiments. From the results of batch sorption experiments, the bead-type polysulfone carrier with only 5% HNO₃-treated bamboo charcoal (P-5NBC) represented the high Cs removal efficiency of 57.8% for 1 hour sorption time. The Cs removal efficiency of P-5NBC in water after 24 hours reaction maintained > 69% at a wide range of pH and temperature conditions, attesting to its applicability under various water systems. Batch sorption experiments were repeated for P-5NBC coated with two cultivated microorganisms (Pseudomonas fluorescens and Bacillus drentensis), which were typical indigenous species inhabited in soil and groundwater. The Cs removal efficiency for two microorganisms coated polysulfone carrier (BP-5NBC) additionally increased by 19% and 18%, respectively, compared to that of only P-5NBC without microorganisms coated. The average Cs desorption rate of P-5NBC for 24 h was lower than 16%, showing the Cs was stably attached on HNO₃-treated bamboo charcoal in so much as its long-term use. The maximum Cs sorption capacity (q_m) of P-5NBC calculated from the Langmuir isotherm model study was 60.9 mg/g, which was much higher than those of other adsorbents from previous studies for 1 h sorption time. The results of continuous column experiments showed that the P-5NBC coated with microorganisms packed in the column maintained > 80% of the Cs removal efficiency during 100 pore volumes flushing. It suggested that only 14.7 g of P-5NBC (only 0.75 g of HNO₃ treated bamboo charcoal included) can successfully clean-up 7.2 L of Cs contaminated water (the initial Cs concentration: 1 mg/L; the effluent concentration: < 0.2 mg/L). The present results suggested that the Cs contaminated water can be successfully cleaned up by using a small amount of the polysulfone carrier with HNO₃-treated bamboo charcoal.

• Genomics & Informatics
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• v.8 no.2
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• pp.76-80
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• 2010

Although autism spectrum disorder (ASD) has been thought to have a substantial genetic background, major contributing genes have yet to be identified or successfully replicated. Immunological dysfunction has been suggested to be associated with ASD, and T cell-mediated immunity was considered important for the development of ASD. In this study, we analyzed 163 ASD subjects and 97 normal controls by genomic quantitative PCR to evaluate the association between the copy number variation of the 7q34 locus, harboring the TCRB gene, and ASDs. As a result, there was no significant difference of the frequency distribution of TCRB copy numbers between ASD cases and normal controls. TCRB gene copy numbers ranged from 0 to 5 copies, and the frequency distribution of each copy number was similar between the two groups. The proportion of the individuals with <2 copies of TCRB was 52.8% (86/163) in ASD cases and 57.1% (52/91) in the control group (p=0.44). The proportion of individuals with >2 copies of TCRB was 11.7% (19/163) in ASD cases and 12.1% (11/91) in the control group (p=0.68). After the effects of sex were adjusted by logistic regression, ORs for individuals with <2 copies or >2 copies showed no significant difference compared with the diploid copy number as reference (n=2). Although we could not see the positive association, our results will be valuable information for mining ASD-associated genes and for exploring the role of T cell immunity further in the pathogenesis of ASD.

• PNF and Movement
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• v.11 no.2
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• pp.9-19
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• 2013

Purpose : Study on the effects of attention(working memory) for normal adults by applying transcranial Direct Current Stimulation(tDCS). Methods : There are two groups of 10 random people aged between 20 to 30, one is an experimental group(stimulation) and the other is a comparison group(simulate stimulation). For the 1st da y, pre-stimulation test is executed, and for the 2nd day to the 4th day, tDCS is applied stimulated both on the right prefrontal lobe and the left occipital lobe through the electrode. The stimulation lasts for 15 minutes with voltage power of 1mA for each day. The post-stimulation test is executed on the day of 5th. In this paper, a FAIR attention test is used for measuring the attention. Results : As the result of the experiment, there are significant gaps both on the value of P, the ability of selective attention, and the value of C, the consistent ability after the stimulation on right prefrontal and left occipital lobe. And there is no significant gap on the value Q, the quality(Control). However, all the P, Q, and C values are increased according to the average value between the pre-stimulation test and the post-stimulation. Conclusion : Finally, ability of selective attention and self-control, and consistent attention is progressed by applying the tDCS to the normal people.

• Journal of Microbiology and Biotechnology
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• v.15 no.5
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• pp.1047-1053
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• 2005

Cyclosporin A binding protein type-19 kDa peptidyl-prolyl cis/trans isomerase (PPIases, EC 5.2.1.8) of Euglena gracilis was purified and some of its biochemical characters were elucidated. Purification of the PPIase was achieved by employing a series of steps involving ammonium sulfate precipitation, Superdex G-75 gel filtration chromatography, Mono­Q anion and Mono-S cation exchange chromatographies, and Superdex S-200 gel filtration chromatography on FPLC. Purified PPIase had a specific activity of 8,250 units/mg, showing a 27-fold increase compared with that of cell-free extract of Euglena gracilis. The enzyme consisted of a single polypeptide chain with a molecular mass of 19 kDa. It showed high substrate specificity to succinyl-Ala-Ala-Pro-Phe-p-nitroanilide, and $k_{car}/K_{m}$, for this substrate was found to be $61.19{\times}10^5/sec$. The isomer distributions were investigated at an equilibrium of seven different peptide substrates, varying Xaa in Suc-Ala-Xaa-Pro-Phe-p-nitroanilide in dimethylsulfoxide. The cis/trans equilibrium constants were estimated to be from 0.14 (Ile) to 0.63 (Gly), which correspond to $12.00\%\;to\;38.52\%$ of the cis population, respectively, under experimental condition. The enzyme was highly sensitive to the immunosuppressive ligand cyclosporin A, but not to other immunosuppressants such as FK506 and rapamycin. Thus, it appears to belong to the class of cyclophilin.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• v.2 no.2
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• pp.149-158
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• 1998

Biomass of non-living brown seaweed Sargassun fluitans pretreated with NaOH is capable of taking up more than $10\%$ $(q_{max}$ : 3.85 mmol/g for Al and 1.48 mmol/g for Cu) of its dry weight in the Al and Cu at pH of 4.5. However, the maximum Al and Cu uptakes calculated from Langmuir isotherm were 1.58 mmol/g for Al and 1.35 mmol/g for Cu at pH 3.5. Equilibrium batch sorption study was performed using two-metal system containing Al and Cu. The mathematical model of the two-metal sorption system enabled quantitative estimation of one-metal biosorption inhibition due to the influence of a second metal. NaOH-treated S. fluitans contained 2.19 mmol $(43\;wt.\%)$ carboxyl groups per gram of biomass. A modified form of Langmuir, which assumes binding of Cu as $Cu^{2+}$ and Al as $Al(OH)_2^+,$ was used to model the experimental data. This result agrees with the one of mono-valent sorption for Al in single-metal system. The modified Langmuir model gives the following affinity correlated coefficients: 0.196 for Cu and 6.820 for Ah at pH 4.5, and 2.904 for Cu and 3.131 for Al at pH 3.5. The interference of Al in Cu biosorptive uptake was assessed by `cutting' the three dimensional uptake isotherm surfaces at constant second-metal final concentrations. Equimolar final equilibrium concentrations of Cu and Al of 1 mM at pH 4.5 give Cu and hi uptakes reduced by $82.5\%\;and\;5.4\%,$ respectively. However, these values at pH 3.5 were $55\%\;(Cu)\;and\;31\%$ (Al).

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.8
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• pp.1065-1071
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• 2013

The swine leukocyte antigen class II molecules are possibly associated with the induction of protective immunity. The study described here was to investigate the relationship between polymorphisms in exon 2 of the swine DQA gene and piglet diarrhea. This study was carried out on 425 suckling piglets from three purebred pig strains (Large White, Landrace and Duroc). The genetic diversity of exon 2 in swine DQA was detected by PCR-SSCP and sequencing analysis, eight unique SSCP patterns (AB, BB, BC, CC, CD, BD, BE and DD) representing five specific allele (A to E) sequences were detected. Sequence analysis revealed 21 nucleotide variable sites and resulting in 12 amino acid substitutions in the populations. A moderate level polymorphism and significant deviations from Hardy-Weinberg equilibrium of the genotypes distribution were observed in the populations (p<0.01). The association analysis indicated that there was a statistically significant difference in the score of piglet diarrhea between different genotypes, individuals with genotype CC showed a lower diarrhea score than genotypes AB ($0.98{\pm}0.09$), BB ($0.85{\pm}0.77$) and BC ($1.25{\pm}0.23$) (p<0.05), and significantly low than genotype BE ($1.19{\pm}0.19$) (p<0.01), CC genotype may be a most resistance genotype for piglet diarrhea.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.19 no.1
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• pp.10-19
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• 1986

A study on the rapid fermentation of fish sauce has been carried out for effective utilization of sardine. The frozen sardine was thawed at room temperature, chopped, homogenized with equal amount of water and then hydrolyzed by addition of commercial proteolytic enzymes such as bromelain, papaya protease, ficin and a enzyme mixture under different conditions of hydrolysis. The effect of wheat gluten for masking fishy odor and color development during thermal treatment were also tested. The reaction mixture was heated for 30 minutes at $100^{\circ}C$ for enzyme inactivation, pasteurization and color development and then centrifuged for 20 minutes at 4,000 rpm. Finally, table salt and benzoic acid were added for bacteriostatic effect. The results were summarized as follows ; 1. The hydrolyzing temperature, time, pH and the concentration of enzymes based on the weight of whole sardine for optimal hydrolysis were as follows: autolysis, $52.5^{\circ}C$, 4 hours, pH 8.0: with $0.25\%$ bromelain, $52.5^{\circ}C$, 4 hours, pH 6.6 :with $0.25\%$ ficin, $52.5^{\circ}C$, 4 hours, pH 6.8: with $0.3\%$ papaya protease, $52.5^{\circ}C$, 4 hours, pH 6.6: with $6\%$ enzyme mixture, $52.5^{\circ}C$, 4 hours, pH 6.9, respectively. But pH control was not much beneficial in increasing yield. 2. The hydrolytic reaction of chopped sardine with proteolytic enzymes could be interpreted as a first order reaction that devided into 2 periods with different reaction rate constsnts. $Q_{10}$ values of the first period prior to 4 hours were 1.23 to 1.31, and those of post 4 hours were 1.25 to 1.55. The corresponding activation energies were $1.81{\times}10^4\;to\;2.34{\times}10^4\;kJ/kmol$ and $1.92{\times}10^4\;to\;3.77{\times}10^4\;kJ/kmol$, respectively. 3. The reasonable amount of $75\%$ vital wheat gluten for addition was $9\%$ of chopped sardine. 4. The dark brown color was mainly developed during the thermal treatment for 30 minutes at $100^{\circ}C$ and not changed during storage.

• Magazine of the Korean Society of Agricultural Engineers
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• v.23 no.3
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• pp.78-87
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• 1981

This study was attempted to get dimensionless unit hydrograph by linear model which can be used to the estimation of flood for the development of Agricultural water resources and laid emphasis on the application of dimensionless unit hydrographs for the ungaged watersheds by applying linear model. The results summarized through this study are as follows. 1.Peak discharge is found to be Qp= CAR (C =0. 895A-o.145) having high significance between peak discharge, Qp and effective rainfall, R within the range of small watershed area, 84 to 470km2. consequently, linearity was acknowledged between rainfall and runoff. Reasonability is confirmed for the derivation of dimensionless unit hydrograph by linear model. 2.Through mathematical analysis, formula for the derivation of dimensionless unit hydrograph was derived. qp--p=(tp--t)n-1[e-(n-1)](tp--t-1) 3.Moment method was used for the evaluation of storage constant, K and shape parameter, n for the derivation of dimensionless unit hydrograph. Storage constant, K is more closely related with the such watershed characteristics as length of main stream and slopes. On the other hand, the shape parameter, n was derived with such watershed characteristics as watershed area, river length, centroid distance of the basin and slopes. 4.Time to peak discharge, Tp could be expressed as Tp=1. 25 (√s/L)0.76 having a high significance. 5.Dimensionless unit hydrographs by linear model stood more closely to the observe dimensionless unit hydrographs On the contrary, dimensionless unit hydrographs by S.C. S. method has much difference in comparison with linear model at the falling limb of hydrographs. 6.Relative errors in the q/qp at the point of 0.8 and 1.2 for the dimensionles ratio by linear model and S. C. S. method showed to be 2.41, 1.57 and 4.0, 3.19 percent respectively to the q/qp of observed dimensionless unit hydrographs. 7.Derivation of dimensionless unit hydrograph by linear model can be accomplished by linking the two empirical formulars for storage constant, K, and shape parameter, n with derivation formular for dimensionless unit hydrograph for the ungaged small watersheds.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.4
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• pp.1397-1401
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• 2015

Background: To determine the expressions of Tbx3, a member of subgroup belonging to T-box family, and its prognostic value in pancreatic carcinoma. Materials and Methods: We determined the expression levels of Tbx3 on both mRNA and protein levels in 30 pairs of fresh tumor tissues and paratumor tissues by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting, respectively. In addition, protein level of Tbx3 were identified using immunochemistry in 80 pairs of paraffin-embedded specimen. The correlations between Tbx3 expression and various clinicopathological parameters as well as overall survival were evaluated. Results: Tbx3 mRNA and protein levels in tumor tissues were significantly higher than in the paratumor tissues by qRT-PCR ($0.05{\pm}0.007$ vs. $0.087{\pm}0.001$, p<0.001) and western blotting ($1.134{\pm}0.043$ vs. $0.287{\pm}0.017$, p<0.001). The statistical analysis based on immunohistochemical evaluation suggested that Tbx3 aberrant expression was significantly associated with several conventional clinicopathological variables, such as gender, age, tumor position, preoperative CA19-9 level, pathological T staging and N staging. Univariate and multivariate analyses revealed that Tbx3 expression was an independent prognostic factor for overall survival (<0.001). Conclusions: Our results suggest that overexpression of Tbx3 is associated with poor prognosis of pancreatic cancer patients. However, additional clinical trials are needed to accurately validate this observation.